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The map label for this gene is thiH [H]

Identifier: 85058114

GI number: 85058114

Start: 226321

End: 227157

Strand: Reverse

Name: thiH [H]

Synonym: SG0136

Alternate gene names: 85058114

Gene position: 227157-226321 (Counterclockwise)

Preceding gene: 85058115

Following gene: 85058104

Centisome position: 5.45

GC content: 60.22

Gene sequence:

>837_bases
ATGACCAGCTTTACCCCGCACTGGCAGCGGATTCAATGGGACGACGTCGGCATGCGTATCCACAGCCAGACGGCCCGCGA
CGTCGAACGGGCGCTGGCGGCGGAAAACCCCGGACCGGCGGAAATGATGGCGCTACTGTCGCCGGCGGCACTGCCCTATC
TGGAGCCGCTGGCTCAGCGAGCGCAGCGGTTGACCCGCCAACGCTTTGGCAACGCCATCAATTTTTACTTGCCCCTTTAC
CTGTCAAATCTCTGCGCCAACGACTGTACTTACTGCGGCTTTTCCATGAGCAACCGCATCCGCCGCAGGACGCTGGATGA
ACAGAAAATCCTCAGCGAGTGCCGGGCTATCCGCGCCAAGGGCATTGACAACGTGCTGCTGGTTACCGGCGAGCATAAAA
GTAAAGTGGGCATCGACTATTTTCGCCGTTACGTGCCGCTGGTACGCCAGGAATTCAGCTCGCTGATGATGGAGGTGCAA
CCGCTATCACAGGCGAAATACGCCGAGCTGAAAGGCCTGGGGCTGGACGGCGTGCTGGTGTATCAGGAAACCTATCATCC
GCCCACCTACCGGCACCATCATTTGCGCGGGCAGAAACAGGATTTTGTCTGGCGGCTTGAAACGCCGGATCGTCTGGCGC
GGGCCGGGATCGACAAGATCGGCCTCGGCGTGCTAATCGGCCTTTCTGATAACTGGCGCGCCGACTGCTATATGGTGGCC
CGTCACCTGCTGTATATGCGCCACACCTACTGGCAGAGCCGCTATTCGCTGTCGTTCCCCCGGCTGCGTCCCTGTACCGG
CGGCGTAATGCCGGCCTCTTTAATGGACGAAGCCTAG

Upstream 100 bases:

>100_bases
CTCGCTTTCCGGGTCGCGCTGGAAGCCGGCGAATTGGCCCGTCAGGCCAGCGCCACCAGCCCGCTGACCGGCTTTCTAAC
CCCCCGGCGGAGGCTCAAGC

Downstream 100 bases:

>100_bases
CTGTTGCAGGTCATGTGCGCTTTCCGCCTGCTGGCGCCGGAGGTGGAAATTTCCCTATCCACCCGCGAATCCGCCCATTT
TCGCAATCATGCGGTCCCTA

Product: putative thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTSFTPHWQRIQWDDVGMRIHSQTARDVERALAAENPGPAEMMALLSPAALPYLEPLAQRAQRLTRQRFGNAINFYLPLY
LSNLCANDCTYCGFSMSNRIRRRTLDEQKILSECRAIRAKGIDNVLLVTGEHKSKVGIDYFRRYVPLVRQEFSSLMMEVQ
PLSQAKYAELKGLGLDGVLVYQETYHPPTYRHHHLRGQKQDFVWRLETPDRLARAGIDKIGLGVLIGLSDNWRADCYMVA
RHLLYMRHTYWQSRYSLSFPRLRPCTGGVMPASLMDEA

Sequences:

>Translated_278_residues
MTSFTPHWQRIQWDDVGMRIHSQTARDVERALAAENPGPAEMMALLSPAALPYLEPLAQRAQRLTRQRFGNAINFYLPLY
LSNLCANDCTYCGFSMSNRIRRRTLDEQKILSECRAIRAKGIDNVLLVTGEHKSKVGIDYFRRYVPLVRQEFSSLMMEVQ
PLSQAKYAELKGLGLDGVLVYQETYHPPTYRHHHLRGQKQDFVWRLETPDRLARAGIDKIGLGVLIGLSDNWRADCYMVA
RHLLYMRHTYWQSRYSLSFPRLRPCTGGVMPASLMDEA
>Mature_277_residues
TSFTPHWQRIQWDDVGMRIHSQTARDVERALAAENPGPAEMMALLSPAALPYLEPLAQRAQRLTRQRFGNAINFYLPLYL
SNLCANDCTYCGFSMSNRIRRRTLDEQKILSECRAIRAKGIDNVLLVTGEHKSKVGIDYFRRYVPLVRQEFSSLMMEVQP
LSQAKYAELKGLGLDGVLVYQETYHPPTYRHHHLRGQKQDFVWRLETPDRLARAGIDKIGLGVLIGLSDNWRADCYMVAR
HLLYMRHTYWQSRYSLSFPRLRPCTGGVMPASLMDEA

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=277, Percent_Identity=70.3971119133574, Blast_Score=423, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 32066; Mature: 31935

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSFTPHWQRIQWDDVGMRIHSQTARDVERALAAENPGPAEMMALLSPAALPYLEPLAQR
CCCCCCCCCEEEEHHHCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNAINFYLPLYLSNLCANDCTYCGFSMSNRIRRRTLDEQKILSECRAIRAK
HHHHHHHHHCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
GIDNVLLVTGEHKSKVGIDYFRRYVPLVRQEFSSLMMEVQPLSQAKYAELKGLGLDGVLV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
YQETYHPPTYRHHHLRGQKQDFVWRLETPDRLARAGIDKIGLGVLIGLSDNWRADCYMVA
EEECCCCCCCCHHHCCCCCCCCEEEECCHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHH
RHLLYMRHTYWQSRYSLSFPRLRPCTGGVMPASLMDEA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCC
>Mature Secondary Structure 
TSFTPHWQRIQWDDVGMRIHSQTARDVERALAAENPGPAEMMALLSPAALPYLEPLAQR
CCCCCCCCEEEEHHHCCEEHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
AQRLTRQRFGNAINFYLPLYLSNLCANDCTYCGFSMSNRIRRRTLDEQKILSECRAIRAK
HHHHHHHHHCCEEEHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHC
GIDNVLLVTGEHKSKVGIDYFRRYVPLVRQEFSSLMMEVQPLSQAKYAELKGLGLDGVLV
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEE
YQETYHPPTYRHHHLRGQKQDFVWRLETPDRLARAGIDKIGLGVLIGLSDNWRADCYMVA
EEECCCCCCCCHHHCCCCCCCCEEEECCHHHHHHCCCHHCCCEEEEECCCCCCHHHHHHH
RHLLYMRHTYWQSRYSLSFPRLRPCTGGVMPASLMDEA
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609 [H]