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The map label for this gene is ntrB [H]

Identifier: 84625882

GI number: 84625882

Start: 4776503

End: 4777558

Strand: Reverse

Name: ntrB [H]

Synonym: XOO_4225

Alternate gene names: 84625882

Gene position: 4777558-4776503 (Counterclockwise)

Preceding gene: 84625883

Following gene: 84625881

Centisome position: 96.71

GC content: 68.28

Gene sequence:

>1056_bases
ATGTTGACATCCTCCTCCATGCCATCGCTGGACAGTCTGGGCACGCCGGTGGCCTGGGCCGACCGCGAGGGTCGCCTGCT
GGGCGTCAACCCGGCGTTCGCGCGCTGGCTCGGGGTCGGTGCCCGGCGGTTGGTCGAACAACCCCTGGCCGCGCTGGAAG
TGCAGGGCGATGCGCTTGCACGCTTTCTGCTCAACGACGAGCGTGATGTGTTGCGTCTGCACCGGCTGGCGCTGGGCCTG
CCCAACGACGCGCCACGCTTTGCCGAAGGCTGGCTGTCGCGCCTGGACGACGGCGGCTGGTTGCTGGAAACCCACCCGGT
CGACGAATTCCCCGCGCTGGATCCCACCCACGCCTTGCCCAATGCCTTGAGCGCCGCGCTCAAGGGGCTGGCGCACGAGT
TACGCAACCCATTGGCCGGACTGAAGGGCGCCGCCCAGCTACTCGCACGCCGCTCGGCGGGACGCGACGAAGACGAGCGC
GAGCTGCTCGAGCTGATCGGCACCGAAATCGAACGCCTCAACACCTTGCTCGAACGGCTGCTCTCGCCAACCCCGGCGCG
GCCGCATGACCGCCTCAACATCCATGTGGTCCTTGAACGTGTGCTGCGCCTGGCCGAAGCCGAGGGCGGCTGGTCGGTGC
AGGTGCAGCGCGACTACGACCCCAGCATTCCCGACATTCTGGGCGATGGCGACCGCCTGACCCAGGCGGTGTGGAATCTG
GTACGCAACGCCTTCCAGGCTGGCGCCACCCGTGTGACCCTGCGCACCCGCGTCGAACATGGCCAACGCATCCGCGACCG
CGTGCACGCGATGTCGCTGCGGCTGGAAATCATCGACGACGGCGGCGGCGTGCCGGAAGAACTGGCCGAGCACCTGTTCC
TGCCGCTGGTCAGCGGCCGTGCCGAAGGCAGCGGATTGGGTCTGGCGCTGGCCCAGCAGGTCTCGCGCCAACACCACGGC
ACGCTGACCTACCGCTCGCGTCCGGGCCATACCGTTTTCACCCTGGTGCTGCCCGAGCTGGCCGGCAATGACGACAAGGA
GCATCTGCATGGCTGA

Upstream 100 bases:

>100_bases
TCGGTGCGACCCGCCGCTGTCCGGCCCCTATGTTGTTTACGGACGCTTCGCTTTGCGCGACATTGATGCACGGCGCTGCC
GTTTGCACTAAATTGGTGCA

Downstream 100 bases:

>100_bases
GGCCGCCGCCGTATCCCACATCTGGGTGGTCGACGATGACCGGTCGGTCCGTTTCGTGCTGTCCACCGCCTTGCGCGATG
CCGGTTACGCGGTGGATGGT

Product: two-component system sensor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLTSSSMPSLDSLGTPVAWADREGRLLGVNPAFARWLGVGARRLVEQPLAALEVQGDALARFLLNDERDVLRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRSAGRDEDER
ELLELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSRQHHG
TLTYRSRPGHTVFTLVLPELAGNDDKEHLHG

Sequences:

>Translated_351_residues
MLTSSSMPSLDSLGTPVAWADREGRLLGVNPAFARWLGVGARRLVEQPLAALEVQGDALARFLLNDERDVLRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRSAGRDEDER
ELLELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSRQHHG
TLTYRSRPGHTVFTLVLPELAGNDDKEHLHG
>Mature_351_residues
MLTSSSMPSLDSLGTPVAWADREGRLLGVNPAFARWLGVGARRLVEQPLAALEVQGDALARFLLNDERDVLRLHRLALGL
PNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALPNALSAALKGLAHELRNPLAGLKGAAQLLARRSAGRDEDER
ELLELIGTEIERLNTLLERLLSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGRAEGSGLGLALAQQVSRQHHG
TLTYRSRPGHTVFTLVLPELAGNDDKEHLHG

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=347, Percent_Identity=35.7348703170029, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1788549, Length=224, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790436, Length=216, Percent_Identity=31.9444444444444, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789808, Length=203, Percent_Identity=26.1083743842365, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994928, Length=369, Percent_Identity=23.5772357723577, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1786600, Length=246, Percent_Identity=28.8617886178862, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790346, Length=216, Percent_Identity=28.2407407407407, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38593; Mature: 38593

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTSSSMPSLDSLGTPVAWADREGRLLGVNPAFARWLGVGARRLVEQPLAALEVQGDALA
CCCCCCCCCHHHCCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFLLNDERDVLRLHRLALGLPNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALP
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHH
NALSAALKGLAHELRNPLAGLKGAAQLLARRSAGRDEDERELLELIGTEIERLNTLLERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGR
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCC
AEGSGLGLALAQQVSRQHHGTLTYRSRPGHTVFTLVLPELAGNDDKEHLHG
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEHHHCCCCCHHHCCC
>Mature Secondary Structure
MLTSSSMPSLDSLGTPVAWADREGRLLGVNPAFARWLGVGARRLVEQPLAALEVQGDALA
CCCCCCCCCHHHCCCCCEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHH
RFLLNDERDVLRLHRLALGLPNDAPRFAEGWLSRLDDGGWLLETHPVDEFPALDPTHALP
HHHHCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCHHHHH
NALSAALKGLAHELRNPLAGLKGAAQLLARRSAGRDEDERELLELIGTEIERLNTLLERL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
LSPTPARPHDRLNIHVVLERVLRLAEAEGGWSVQVQRDYDPSIPDILGDGDRLTQAVWNL
CCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHCCCHHHHHHHHHH
VRNAFQAGATRVTLRTRVEHGQRIRDRVHAMSLRLEIIDDGGGVPEELAEHLFLPLVSGR
HHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHHCCC
AEGSGLGLALAQQVSRQHHGTLTYRSRPGHTVFTLVLPELAGNDDKEHLHG
CCCCCHHHHHHHHHHHHHCCEEEEECCCCCEEEEEEEHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]