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The map label for this gene is amtB [H]

Identifier: 84625883

GI number: 84625883

Start: 4777829

End: 4779304

Strand: Reverse

Name: amtB [H]

Synonym: XOO_4226

Alternate gene names: 84625883

Gene position: 4779304-4777829 (Counterclockwise)

Preceding gene: 84625884

Following gene: 84625882

Centisome position: 96.74

GC content: 66.33

Gene sequence:

>1476_bases
ATGCGCTCTAACCCCACCAGCCTTGGAGTTCGTATGAAGACAGCTCTTTTTGCCGGGTGGAAAGCCCGGTTCTACAGCGC
GTGCATGCTGATGCTGTTCAGCGCGCTGATCGTCGGTGGCATGGGCAGCGCGCAGGCGCAGTCCACTCCCCAGATCACCC
CACTGCCGACCGCGCCGGTCACCACCGAACCGCTGCCGTCGGCGCAACCGGCCGCCCCGCCGGCCGCTGCCGTCGAGGCC
GCACCGGTGGTGGAGAAGGGCGACGTCGCCTGGATGCTGACTTCCACCTTGCTGGTGCTGCTCATGGTGGTGCCGGGCCT
GGCGCTGTTCTACGGCGGCATGGTGCGCGCCAAGAACGTGTTGTCGGTGCTGATCCAGGTCGCTGTGGTGTTCTCGCTGC
TGATCATCCTGTGGGTGCTGTACGGCTACAGCCTGGCGTTCTCCGGTGGAGGTGCGTGGATCGGCAATCTGGACAAGGCG
CTGCTCAAGGGCGTCACCATCGAAACCCTGGCGGCCACCTTCACCAAGGGCGTGAGCCTGCCGGAATACGTGTTTGTGGT
GTTCCAGGCGACCTTCGCCGGCATTACCGGCGCGCTGATCGTCGGCGCCTTTGCCGAACGCGCCAAGTTCGGCGCGGTGC
TGTTGTTCTCGGCGATCTGGTTCACCTTCGGCTACCTGCCGCTGGCGCACATGGTCTGGGCCACCGGTGGGTATCTGTTC
GGGTTGGGCGCGCTGGATTTCGCAGGCGGTACCGTGGTGCATATCAACGCCGGTGTCGCCGGTCTGGTTGGCGCCTACTT
CGTCGGCAAGCGTGCCGGGTTGGGACGTGAGGCGATCAAGCCGCACAACGTGACCCTGACCTTCATCGGTGCCTCGCTGC
TGTGGGTGGGCTGGTTCGGTTTCAACGCCGGTTCCAATCTGGAAGCCAATGCCGGTGCGGCGCTGGCCTTCCTCAACACC
TTGCTGGCGACCGCTGCCGCCATCCTGGGCTGGTCGCTGACCGAGAAGATCATCAAGGGCAAGTCGTCCGCCCTGGGTGT
GGCCTCGGGCATGGTGGCCGGCCTGGTCGGCATCACCCCGGCCTGCGGTACGGTGGGCCCGTTCGGCGCGATCGTCATTG
GCCTGGCTGCCGGCGTGCTCTGCGTGTGGGGCGTCACCGGTCTGAAGCGCCTGCTCGGTGCCGACGACAGCCTGGACGTG
TTCGGCGTGCATGGCCTGGGCGGCATCATCGGCGCGATCCTGACCGGCGTGTTCTCGGCTGCCTCGCTGGGCGGCCAGAA
GGGCGGCGATTACGACATCGTTCATCAGGTCGGCATCCAGGCGCTGGGCGTCGGAATCACGGTCGGCTGGATCGGCGTGG
TGTCGGTGGTGGGGTTTGTGGTCGCCAAGCTGGTATTCGGCCTGCGCGTGAGCGAAGAAGCCGAGCGCGAGGGCCTGGAC
ATCACCTCGCACGGCGAAGCCGCGTACGAGTCCTGA

Upstream 100 bases:

>100_bases
ATCCAGAACGCCGCCGGCACCGGCAAGATCGGCGACGGCAAGATCTTCGTCACCGCGATCGAGCAGGTGGTGCGCATCCG
CACCGGCGAGATCGGCGCCG

Downstream 100 bases:

>100_bases
GCATGCACACCCGCGGCCATGCTGCTGCGCTGCGGGTGGCGCAACGACCCGGCCGGGAATGCCCAGCCGGGTCCGTTTTC
CCCCTTGCCCCGTTGGGCGC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MRSNPTSLGVRMKTALFAGWKARFYSACMLMLFSALIVGGMGSAQAQSTPQITPLPTAPVTTEPLPSAQPAAPPAAAVEA
APVVEKGDVAWMLTSTLLVLLMVVPGLALFYGGMVRAKNVLSVLIQVAVVFSLLIILWVLYGYSLAFSGGGAWIGNLDKA
LLKGVTIETLAATFTKGVSLPEYVFVVFQATFAGITGALIVGAFAERAKFGAVLLFSAIWFTFGYLPLAHMVWATGGYLF
GLGALDFAGGTVVHINAGVAGLVGAYFVGKRAGLGREAIKPHNVTLTFIGASLLWVGWFGFNAGSNLEANAGAALAFLNT
LLATAAAILGWSLTEKIIKGKSSALGVASGMVAGLVGITPACGTVGPFGAIVIGLAAGVLCVWGVTGLKRLLGADDSLDV
FGVHGLGGIIGAILTGVFSAASLGGQKGGDYDIVHQVGIQALGVGITVGWIGVVSVVGFVVAKLVFGLRVSEEAEREGLD
ITSHGEAAYES

Sequences:

>Translated_491_residues
MRSNPTSLGVRMKTALFAGWKARFYSACMLMLFSALIVGGMGSAQAQSTPQITPLPTAPVTTEPLPSAQPAAPPAAAVEA
APVVEKGDVAWMLTSTLLVLLMVVPGLALFYGGMVRAKNVLSVLIQVAVVFSLLIILWVLYGYSLAFSGGGAWIGNLDKA
LLKGVTIETLAATFTKGVSLPEYVFVVFQATFAGITGALIVGAFAERAKFGAVLLFSAIWFTFGYLPLAHMVWATGGYLF
GLGALDFAGGTVVHINAGVAGLVGAYFVGKRAGLGREAIKPHNVTLTFIGASLLWVGWFGFNAGSNLEANAGAALAFLNT
LLATAAAILGWSLTEKIIKGKSSALGVASGMVAGLVGITPACGTVGPFGAIVIGLAAGVLCVWGVTGLKRLLGADDSLDV
FGVHGLGGIIGAILTGVFSAASLGGQKGGDYDIVHQVGIQALGVGITVGWIGVVSVVGFVVAKLVFGLRVSEEAEREGLD
ITSHGEAAYES
>Mature_491_residues
MRSNPTSLGVRMKTALFAGWKARFYSACMLMLFSALIVGGMGSAQAQSTPQITPLPTAPVTTEPLPSAQPAAPPAAAVEA
APVVEKGDVAWMLTSTLLVLLMVVPGLALFYGGMVRAKNVLSVLIQVAVVFSLLIILWVLYGYSLAFSGGGAWIGNLDKA
LLKGVTIETLAATFTKGVSLPEYVFVVFQATFAGITGALIVGAFAERAKFGAVLLFSAIWFTFGYLPLAHMVWATGGYLF
GLGALDFAGGTVVHINAGVAGLVGAYFVGKRAGLGREAIKPHNVTLTFIGASLLWVGWFGFNAGSNLEANAGAALAFLNT
LLATAAAILGWSLTEKIIKGKSSALGVASGMVAGLVGITPACGTVGPFGAIVIGLAAGVLCVWGVTGLKRLLGADDSLDV
FGVHGLGGIIGAILTGVFSAASLGGQKGGDYDIVHQVGIQALGVGITVGWIGVVSVVGFVVAKLVFGLRVSEEAEREGLD
ITSHGEAAYES

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=425, Percent_Identity=54.5882352941177, Blast_Score=437, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=29.1954022988506, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17550180, Length=432, Percent_Identity=28.7037037037037, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=436, Percent_Identity=27.0642201834862, Blast_Score=115, Evalue=6e-26,
Organism=Caenorhabditis elegans, GI133931100, Length=450, Percent_Identity=24, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6325396, Length=419, Percent_Identity=36.0381861575179, Blast_Score=236, Evalue=5e-63,
Organism=Saccharomyces cerevisiae, GI6324187, Length=409, Percent_Identity=37.6528117359413, Blast_Score=227, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6321559, Length=418, Percent_Identity=34.9282296650718, Blast_Score=223, Evalue=6e-59,
Organism=Drosophila melanogaster, GI161078314, Length=419, Percent_Identity=28.1622911694511, Blast_Score=106, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 50182; Mature: 50182

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCC
>Mature Secondary Structure
MRSNPTSLGVRMKTALFAGWKARFYSACMLMLFSALIVGGMGSAQAQSTPQITPLPTAPV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
TTEPLPSAQPAAPPAAAVEAAPVVEKGDVAWMLTSTLLVLLMVVPGLALFYGGMVRAKNV
CCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LSVLIQVAVVFSLLIILWVLYGYSLAFSGGGAWIGNLDKALLKGVTIETLAATFTKGVSL
HHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCC
PEYVFVVFQATFAGITGALIVGAFAERAKFGAVLLFSAIWFTFGYLPLAHMVWATGGYLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHE
GLGALDFAGGTVVHINAGVAGLVGAYFVGKRAGLGREAIKPHNVTLTFIGASLLWVGWFG
ECCHHHHCCCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEHHHHHHHHHHC
FNAGSNLEANAGAALAFLNTLLATAAAILGWSLTEKIIKGKSSALGVASGMVAGLVGITP
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCC
ACGTVGPFGAIVIGLAAGVLCVWGVTGLKRLLGADDSLDVFGVHGLGGIIGAILTGVFSA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHH
ASLGGQKGGDYDIVHQVGIQALGVGITVGWIGVVSVVGFVVAKLVFGLRVSEEAEREGLD
HHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCC
ITSHGEAAYES
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]