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The map label for this gene is glnG [H]

Identifier: 84625881

GI number: 84625881

Start: 4775083

End: 4776510

Strand: Reverse

Name: glnG [H]

Synonym: XOO_4224

Alternate gene names: 84625881

Gene position: 4776510-4775083 (Counterclockwise)

Preceding gene: 84625882

Following gene: 84625880

Centisome position: 96.69

GC content: 66.81

Gene sequence:

>1428_bases
ATGGCTGAGGCCGCCGCCGTATCCCACATCTGGGTGGTCGACGATGACCGGTCGGTCCGTTTCGTGCTGTCCACCGCCTT
GCGCGATGCCGGTTACGCGGTGGATGGTTTCGACAGCGCTGCCGCCGCGCTGCAGGCGCTCGCCATGCGGCCCACGCCGG
ACCTGCTGTTCACCGACGTGCGCATGCCCGGCGAAGACGGCCTGACCCTGCTCGACAAGCTCAAAGCCAAACACCCGCAA
CTGCCGGTGATCGTGATGTCGGCCTATACCGACGTTGCCAGCACCGCCGGCGCCTTCCGCGGCGGCGCGCATGAGTTCCT
GTCCAAGCCCTTCGACCTGGACGATGCGGTGGCGCTGGCCGCACGCGGGCTGCCGGATGCCGATGCGGGTGCCGACGAAA
TCTTCGCCACCCGGCTGGCCGAAGGCTCGGCCTCGTTGATCGGCGACACGCCCGCTATGCAGGCTTTGTTTCGCGCGATC
GGACGTCTGGCGCAGGCGCCGCTGTCGGTCTTGATCAACGGCGAAACCGGCACCGGTAAAGAGCTGGTCGCCCACGCGCT
GCACAACGAATCGCCGCGCGCGCGCAAACCCTTCGTCGCGCTCAATACCGCCGCCATTCCGGCCGAATTGCTGGAAAGCG
AGTTGTTCGGCCACGAAACCGGCGCCTTTACCGGCGCGACCAAACGGCATATCGGCCGTTTCGAACAAGCCGATGGCGGC
ACGCTGTTTCTGGACGAAATCGGCGACATGCCGTTGCCGCTGCAGACACGCCTGTTGCGCGTGCTGGCAGAGAACGAATT
CTTCCGCGTCGGTGGGCGCGAATTGATCCGCGTCGATGTGCGTGTGATCGCCGCGACCCACCAGGATCTGGAAGCCTTGG
TCCAACAAGGCCGCTTCCGCGCCGATTTGCTACATCGCCTGGATGTGGTGCGCCTGCAATTGCCGCCGCTGCGCGAACGC
CGTGGCGACATCGCGCAACTGGCCGAGAACTTCCTGACCATGGCCGGGCGCAAGCTCGACATGTTGCCCAAGCGGCTGTC
GTCGGCCGCGTTGGAAGACCTGCGTCAATACGACTGGCCCGGCAACGTCCGCGAACTGGAAAACGTGTGCTGGCGTCTGG
CCGCGCTGGCCACCGCCGACATCATCGACGTGGTGGATGTGGAAGCCGCACTGGCACGTGGTGGACGTCGTCACCGCAGC
GGCCGCAGCGACGGCCAGTGGGACGACATGCTCTCCAGCTGGGCCGCACAACGCCTGAGCGAAGGCGCCCAGGGCCTGCA
CGCCGAAGCGCGCGAACGCCTGGACAAAACCCTGCTCGAAGCCGCACTGCAACTCACCCAGGGCCGCCGCGCCGAAGCTG
CCGCCCGCCTGGGCCTGGGCCGTAACACCGTCACCCGCAAACTCGGTCCGGGGCGCAAACCCCGTTAA

Upstream 100 bases:

>100_bases
CAACACCACGGCACGCTGACCTACCGCTCGCGTCCGGGCCATACCGTTTTCACCCTGGTGCTGCCCGAGCTGGCCGGCAA
TGACGACAAGGAGCATCTGC

Downstream 100 bases:

>100_bases
GGTTGCCCGAGGCCTCTCCCGTTGGAAGGTCTCGTGCTTGAAAAAGAATCGACGCCTAAGCGATACACGCATATCGCAAT
CGCGCATCAGACCACGCGCG

Product: two-component system regulatory protein

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 475; Mature: 474

Protein sequence:

>475_residues
MAEAAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALAMRPTPDLLFTDVRMPGEDGLTLLDKLKAKHPQ
LPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARGLPDADAGADEIFATRLAEGSASLIGDTPAMQALFRAI
GRLAQAPLSVLINGETGTGKELVAHALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVQQGRFRADLLHRLDVVRLQLPPLRER
RGDIAQLAENFLTMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATADIIDVVDVEAALARGGRRHRS
GRSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDKTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKPR

Sequences:

>Translated_475_residues
MAEAAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALAMRPTPDLLFTDVRMPGEDGLTLLDKLKAKHPQ
LPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARGLPDADAGADEIFATRLAEGSASLIGDTPAMQALFRAI
GRLAQAPLSVLINGETGTGKELVAHALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVQQGRFRADLLHRLDVVRLQLPPLRER
RGDIAQLAENFLTMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATADIIDVVDVEAALARGGRRHRS
GRSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDKTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKPR
>Mature_474_residues
AEAAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALAMRPTPDLLFTDVRMPGEDGLTLLDKLKAKHPQL
PVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALAARGLPDADAGADEIFATRLAEGSASLIGDTPAMQALFRAIG
RLAQAPLSVLINGETGTGKELVAHALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGGT
LFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVQQGRFRADLLHRLDVVRLQLPPLRERR
GDIAQLAENFLTMAGRKLDMLPKRLSSAALEDLRQYDWPGNVRELENVCWRLAALATADIIDVVDVEAALARGGRRHRSG
RSDGQWDDMLSSWAAQRLSEGAQGLHAEARERLDKTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKPR

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=460, Percent_Identity=52.8260869565217, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1790437, Length=463, Percent_Identity=42.7645788336933, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1788550, Length=468, Percent_Identity=38.4615384615385, Blast_Score=315, Evalue=3e-87,
Organism=Escherichia coli, GI1788905, Length=469, Percent_Identity=37.953091684435, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI87082117, Length=327, Percent_Identity=46.177370030581, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1789087, Length=239, Percent_Identity=50.6276150627615, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789233, Length=325, Percent_Identity=40.6153846153846, Blast_Score=231, Evalue=8e-62,
Organism=Escherichia coli, GI1786524, Length=251, Percent_Identity=51.792828685259, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI87082152, Length=234, Percent_Identity=52.991452991453, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI87081872, Length=335, Percent_Identity=41.7910447761194, Blast_Score=221, Evalue=6e-59,
Organism=Escherichia coli, GI1787583, Length=229, Percent_Identity=44.1048034934498, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI87081858, Length=337, Percent_Identity=34.4213649851632, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1789828, Length=256, Percent_Identity=34.375, Blast_Score=118, Evalue=9e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 51571; Mature: 51440

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEAAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALAMRPTPDLLFTDV
CCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
RMPGEDGLTLLDKLKAKHPQLPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALA
CCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
ARGLPDADAGADEIFATRLAEGSASLIGDTPAMQALFRAIGRLAQAPLSVLINGETGTGK
HCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
ELVAHALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
HHHHHHHHCCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCC
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVQQGRFR
EEEHHHCCCCCCCHHHHHHHHHHCCCCEECCCHHEEHHHHHHHHHHHHHHHHHHHCCCHH
ADLLHRLDVVRLQLPPLRERRGDIAQLAENFLTMAGRKLDMLPKRLSSAALEDLRQYDWP
HHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GNVRELENVCWRLAALATADIIDVVDVEAALARGGRRHRSGRSDGQWDDMLSSWAAQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHH
EGAQGLHAEARERLDKTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKPR
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure 
AEAAAVSHIWVVDDDRSVRFVLSTALRDAGYAVDGFDSAAAALQALAMRPTPDLLFTDV
CCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEC
RMPGEDGLTLLDKLKAKHPQLPVIVMSAYTDVASTAGAFRGGAHEFLSKPFDLDDAVALA
CCCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCCHHHHHCCCCCCHHHHHHH
ARGLPDADAGADEIFATRLAEGSASLIGDTPAMQALFRAIGRLAQAPLSVLINGETGTGK
HCCCCCCCCCHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCHH
ELVAHALHNESPRARKPFVALNTAAIPAELLESELFGHETGAFTGATKRHIGRFEQADGG
HHHHHHHHCCCCCCCCCEEEEECHHCCHHHHHHHHHCCCCCCCCCHHHHHHCCHHCCCCC
TLFLDEIGDMPLPLQTRLLRVLAENEFFRVGGRELIRVDVRVIAATHQDLEALVQQGRFR
EEEHHHCCCCCCCHHHHHHHHHHCCCCEECCCHHEEHHHHHHHHHHHHHHHHHHHCCCHH
ADLLHRLDVVRLQLPPLRERRGDIAQLAENFLTMAGRKLDMLPKRLSSAALEDLRQYDWP
HHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
GNVRELENVCWRLAALATADIIDVVDVEAALARGGRRHRSGRSDGQWDDMLSSWAAQRLS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHH
EGAQGLHAEARERLDKTLLEAALQLTQGRRAEAAARLGLGRNTVTRKLGPGRKPR
HHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]