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The map label for this gene is dhfrIII [H]

Identifier: 84625198

GI number: 84625198

Start: 4003331

End: 4003825

Strand: Reverse

Name: dhfrIII [H]

Synonym: XOO_3541

Alternate gene names: 84625198

Gene position: 4003825-4003331 (Counterclockwise)

Preceding gene: 84625199

Following gene: 84625188

Centisome position: 81.05

GC content: 63.43

Gene sequence:

>495_bases
ATGAAGGTGATCTTGATCGTCGCCTTCGACCGCAACAACGCCATCGGCCGTGATAACGATCTGCCGTGGAAACTGCCCGA
TGATCTCAAGCGCTTCAAAGCGCTGACGCTGGGCAAGCCGATCCTGATGGGGCGCAAGACGGCGCAGTCTCTCAGGCGTG
CGTTGCCGGGGCGGTTGAATCTGGTGCTGACGCGTTCGGGGCTGGTGCCGTTCGACGGCATGCAGGCGGTGGCATCGGTG
GCGCAGGCGGTCGAACGCGCCGTGCAAGACGGTGCGCAGGAGCTGTGCGTGATCGGTGGCGGGGAGGTATATCGCCTGAC
CATGGAACGTGCCGATCTGCTGGCCGTGACCGAAGTGGACACGGCGGTAGACGATGCCGACACGCATTTTCCGCCGATCG
ACCCGGCGGCGTGGTCGCCTGTGAGGCGTCAGTTACATCCTGCCGACGATCGGCACGCCTATGCGTTTTCGTTCGTGGAT
TACGAACGCCGCTGA

Upstream 100 bases:

>100_bases
CGCTTGGCGGCATTTTTGTGTGCTATGTGGCCGTTTTTTTGTTCGGAATGCTGGGCCGGAAGTCTGCAATGAAAATCTAT
AACGCGCTCTATCCAGACGC

Downstream 100 bases:

>100_bases
GTTATCCGCTATCGAATGCCCGTCATCGCGATGACGACGGGCGCGCCATGTACTCAGTCTTGTGGCGTATCGACTGGCGG
CTTCGCCGGCACCGCAGTGT

Product: dihydrofolate reductase type III

Products: NA

Alternate protein names: Dihydrofolate reductase type III [H]

Number of amino acids: Translated: 164; Mature: 164

Protein sequence:

>164_residues
MKVILIVAFDRNNAIGRDNDLPWKLPDDLKRFKALTLGKPILMGRKTAQSLRRALPGRLNLVLTRSGLVPFDGMQAVASV
AQAVERAVQDGAQELCVIGGGEVYRLTMERADLLAVTEVDTAVDDADTHFPPIDPAAWSPVRRQLHPADDRHAYAFSFVD
YERR

Sequences:

>Translated_164_residues
MKVILIVAFDRNNAIGRDNDLPWKLPDDLKRFKALTLGKPILMGRKTAQSLRRALPGRLNLVLTRSGLVPFDGMQAVASV
AQAVERAVQDGAQELCVIGGGEVYRLTMERADLLAVTEVDTAVDDADTHFPPIDPAAWSPVRRQLHPADDRHAYAFSFVD
YERR
>Mature_164_residues
MKVILIVAFDRNNAIGRDNDLPWKLPDDLKRFKALTLGKPILMGRKTAQSLRRALPGRLNLVLTRSGLVPFDGMQAVASV
AQAVERAVQDGAQELCVIGGGEVYRLTMERADLLAVTEVDTAVDDADTHFPPIDPAAWSPVRRQLHPADDRHAYAFSFVD
YERR

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Escherichia coli, GI1786233, Length=162, Percent_Identity=39.5061728395062, Blast_Score=113, Evalue=7e-27,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18221; Mature: 18221

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVILIVAFDRNNAIGRDNDLPWKLPDDLKRFKALTLGKPILMGRKTAQSLRRALPGRLN
CEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCEE
LVLTRSGLVPFDGMQAVASVAQAVERAVQDGAQELCVIGGGEVYRLTMERADLLAVTEVD
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCEEEEEEECCCCEEEEEHHH
TAVDDADTHFPPIDPAAWSPVRRQLHPADDRHAYAFSFVDYERR
HHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCC
>Mature Secondary Structure
MKVILIVAFDRNNAIGRDNDLPWKLPDDLKRFKALTLGKPILMGRKTAQSLRRALPGRLN
CEEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHCCHHHHHHHHHCCCCEE
LVLTRSGLVPFDGMQAVASVAQAVERAVQDGAQELCVIGGGEVYRLTMERADLLAVTEVD
EEEEECCCCCCCHHHHHHHHHHHHHHHHHCCHHEEEEECCCEEEEEEECCCCEEEEEHHH
TAVDDADTHFPPIDPAAWSPVRRQLHPADDRHAYAFSFVDYERR
HHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840679; 6371010 [H]