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The map label for this gene is ubiH [H]

Identifier: 84625188

GI number: 84625188

Start: 3992286

End: 3993494

Strand: Reverse

Name: ubiH [H]

Synonym: XOO_3531

Alternate gene names: 84625188

Gene position: 3993494-3992286 (Counterclockwise)

Preceding gene: 84625198

Following gene: 84625187

Centisome position: 80.84

GC content: 69.4

Gene sequence:

>1209_bases
ATGACACATCCCCATGACGTTCTGATCGTAGGCGGCGGCCTGGTCGGCTCTAGCCTTGCGATTGCGCTGGACCGCCTCGG
CCTGGATGTCGGGCTGGTGGAGGCCACCCCCGCCGGCTCGCCGCCGGCGGTGTTCGACCAACGCAATCTCAGCTTTGCGG
CGGCCACCGTGAACGCGCTCGGCGTGCTCGGGGTGATGGCCAAGCTGCGCAGCCCGCCCGGGCCGATCCGGCGCATCCAT
GTCAGCCGCGCCGGCGACTTCGGCCGCGTGCAACTGGACGCGGCCGATTACGGCCGCGACGCCTTCGGCCAGGTCGTGGT
GGCGCGCGACTTCGGCGATGCCTTGCAGGCGCGTCTGGACGCATTGACCCATCTGCGCCGTTATCGCCCTGCACGGTGCA
TCGGCGTGGAACCGGTGCAAGACGGCCTGCGCGCGGTGCGCCTGACGACCGAGTCTGGCGAGCAGCGTGTGCGCGCCAAA
CTGGTGGTCGGCGCCGATGGCAGCCATAGCGCGGTACGCGAATTGCTGCATATCGGTACCGACGCCCACGATTTCCTGCA
AACCCTGTTCGTGGCGCGCGTGCGTGCCTCGCGACCGCCCGATGGCACGGCCTGGGAGCGGTTTGGCGAACATGGGCCAA
CCGCCCTGTTGCCGCGCGGCGACCGCCATTACGGTGCGATCCATTGCGTGGCCCGTGCCGAGGCCGGGGCAGTGGCTGCA
CTCGATGACGCTGGCTGGCTGGCCCGCCTGCAGCGTGCCGCCGGCTGGCGCGCCGGGCGCTTCATCGCCACCGGCGAGCG
CAGTGCTTACCCGCTCGTGCAAGTGCTGGCCAACAGCATCATCGCCGAGCGCGTGGTGCTGCTGGGCAATGCCGCGCAGA
CGCTACATCCGATCGGCGCGCAAGGCTTCAACCTGGGCCTACGCGACGCGCTGACGCTTGCCGAATTGATTGAACACGAT
CACAGCGATGCCGGTGCCGGCGCGTTGCTGGCCGACTACTTGGCGCAGCGCCGGGTGGACCGCGAGCACACCATCGGGTT
TTCCAGCGGTCTGGCCCGGCTCACCAGCAACCCGGCACCCCTGCTGCGCCCGTTGCGCAGCCTCGGTTTGTTCGCGACCT
CACAGGCAGCGCCGTTGCAGTCGATGCTGGTCGGCGGCGCCATGGGTTTGCGTGGCGACATACCGCAGCTTTGCCGGGGC
GTCGCATGA

Upstream 100 bases:

>100_bases
CGGTGCGTCCAAGCCATGAAGGCGCAGGCGACAGCGGGCGCGGCAAGATGTCCCGCCCTGCGGCGGCAACGCGCAGGGGG
CGAAGGCTTATCATGTGGGC

Downstream 100 bases:

>100_bases
GCCGACGCAGCACCCGTGATGCCGTGATCGTCGGCGGTGGCGTTGTCGGTGCCGCCTGCGCGCTGGCGCTGACCGATGCC
GGCCTGAGCGTTGCCTTGGT

Product: 2-octaprenyl-6-methoxyphenyl hydroxylase

Products: H2O; 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MTHPHDVLIVGGGLVGSSLAIALDRLGLDVGLVEATPAGSPPAVFDQRNLSFAAATVNALGVLGVMAKLRSPPGPIRRIH
VSRAGDFGRVQLDAADYGRDAFGQVVVARDFGDALQARLDALTHLRRYRPARCIGVEPVQDGLRAVRLTTESGEQRVRAK
LVVGADGSHSAVRELLHIGTDAHDFLQTLFVARVRASRPPDGTAWERFGEHGPTALLPRGDRHYGAIHCVARAEAGAVAA
LDDAGWLARLQRAAGWRAGRFIATGERSAYPLVQVLANSIIAERVVLLGNAAQTLHPIGAQGFNLGLRDALTLAELIEHD
HSDAGAGALLADYLAQRRVDREHTIGFSSGLARLTSNPAPLLRPLRSLGLFATSQAAPLQSMLVGGAMGLRGDIPQLCRG
VA

Sequences:

>Translated_402_residues
MTHPHDVLIVGGGLVGSSLAIALDRLGLDVGLVEATPAGSPPAVFDQRNLSFAAATVNALGVLGVMAKLRSPPGPIRRIH
VSRAGDFGRVQLDAADYGRDAFGQVVVARDFGDALQARLDALTHLRRYRPARCIGVEPVQDGLRAVRLTTESGEQRVRAK
LVVGADGSHSAVRELLHIGTDAHDFLQTLFVARVRASRPPDGTAWERFGEHGPTALLPRGDRHYGAIHCVARAEAGAVAA
LDDAGWLARLQRAAGWRAGRFIATGERSAYPLVQVLANSIIAERVVLLGNAAQTLHPIGAQGFNLGLRDALTLAELIEHD
HSDAGAGALLADYLAQRRVDREHTIGFSSGLARLTSNPAPLLRPLRSLGLFATSQAAPLQSMLVGGAMGLRGDIPQLCRG
VA
>Mature_401_residues
THPHDVLIVGGGLVGSSLAIALDRLGLDVGLVEATPAGSPPAVFDQRNLSFAAATVNALGVLGVMAKLRSPPGPIRRIHV
SRAGDFGRVQLDAADYGRDAFGQVVVARDFGDALQARLDALTHLRRYRPARCIGVEPVQDGLRAVRLTTESGEQRVRAKL
VVGADGSHSAVRELLHIGTDAHDFLQTLFVARVRASRPPDGTAWERFGEHGPTALLPRGDRHYGAIHCVARAEAGAVAAL
DDAGWLARLQRAAGWRAGRFIATGERSAYPLVQVLANSIIAERVVLLGNAAQTLHPIGAQGFNLGLRDALTLAELIEHDH
SDAGAGALLADYLAQRRVDREHTIGFSSGLARLTSNPAPLLRPLRSLGLFATSQAAPLQSMLVGGAMGLRGDIPQLCRGV
A

Specific function: Oxygenase that introduces the hydroxyl group at carbon four of 2-octaprenyl-6-methoxyphenol resulting in the formation of 2-octaprenyl-6-methoxy-1,4-benzoquinone [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=355, Percent_Identity=27.0422535211268, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI32967307, Length=321, Percent_Identity=27.7258566978193, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1789274, Length=391, Percent_Identity=37.3401534526854, Blast_Score=208, Evalue=5e-55,
Organism=Escherichia coli, GI1786883, Length=401, Percent_Identity=30.1745635910224, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1789273, Length=407, Percent_Identity=28.992628992629, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971
- InterPro:   IPR011295 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 42494; Mature: 42363

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHPHDVLIVGGGLVGSSLAIALDRLGLDVGLVEATPAGSPPAVFDQRNLSFAAATVNAL
CCCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
GVLGVMAKLRSPPGPIRRIHVSRAGDFGRVQLDAADYGRDAFGQVVVARDFGDALQARLD
HHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECHHHCCCCCCCCEEEEECHHHHHHHHHH
ALTHLRRYRPARCIGVEPVQDGLRAVRLTTESGEQRVRAKLVVGADGSHSAVRELLHIGT
HHHHHHHCCCCCEECCCHHHHCHHEEEEECCCCHHEEEEEEEEECCCCHHHHHHHHHCCC
DAHDFLQTLFVARVRASRPPDGTAWERFGEHGPTALLPRGDRHYGAIHCVARAEAGAVAA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCEEE
LDDAGWLARLQRAAGWRAGRFIATGERSAYPLVQVLANSIIAERVVLLGNAAQTLHPIGA
ECCHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
QGFNLGLRDALTLAELIEHDHSDAGAGALLADYLAQRRVDREHTIGFSSGLARLTSNPAP
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCCHH
LLRPLRSLGLFATSQAAPLQSMLVGGAMGLRGDIPQLCRGVA
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCC
>Mature Secondary Structure 
THPHDVLIVGGGLVGSSLAIALDRLGLDVGLVEATPAGSPPAVFDQRNLSFAAATVNAL
CCCCEEEEECCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHH
GVLGVMAKLRSPPGPIRRIHVSRAGDFGRVQLDAADYGRDAFGQVVVARDFGDALQARLD
HHHHHHHHHCCCCCCCEEEEEECCCCCCEEEECHHHCCCCCCCCEEEEECHHHHHHHHHH
ALTHLRRYRPARCIGVEPVQDGLRAVRLTTESGEQRVRAKLVVGADGSHSAVRELLHIGT
HHHHHHHCCCCCEECCCHHHHCHHEEEEECCCCHHEEEEEEEEECCCCHHHHHHHHHCCC
DAHDFLQTLFVARVRASRPPDGTAWERFGEHGPTALLPRGDRHYGAIHCVARAEAGAVAA
CHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEECCCCCCCCCCEEEEEECCCCCEEE
LDDAGWLARLQRAAGWRAGRFIATGERSAYPLVQVLANSIIAERVVLLGNAAQTLHPIGA
ECCHHHHHHHHHHCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCC
QGFNLGLRDALTLAELIEHDHSDAGAGALLADYLAQRRVDREHTIGFSSGLARLTSNPAP
CCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCHHHCCCHHHHHHHHCCCCHH
LLRPLRSLGLFATSQAAPLQSMLVGGAMGLRGDIPQLCRGVA
HHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-octaprenyl-6-methoxyphenol; O2 [C]

Specific reaction: 2-octaprenyl-6-methoxyphenol + O2 = H2O + 2-octaprenyl-6-methoxy-1,4-benzoquinone [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1339425; 9278503 [H]