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The map label for this gene is sbp [H]

Identifier: 84625142

GI number: 84625142

Start: 3950531

End: 3951628

Strand: Reverse

Name: sbp [H]

Synonym: XOO_3485

Alternate gene names: 84625142

Gene position: 3951628-3950531 (Counterclockwise)

Preceding gene: 84625145

Following gene: 84625141

Centisome position: 79.99

GC content: 63.66

Gene sequence:

>1098_bases
ATGTCATTTCACCGCCTGCGTGACGCTGCGCAGCATGGCATGCTTGCTGTTCAGCTGTGCTCAACCGGCCAGGAGATTGT
CGTGACATACCCCCGTATCCGTCTTGCTTGCCTTCTTTGCACCCTGCTGGTCCTGGCCGGCCCGGCAGGCGCACGCGAGA
TCGGGCTGCTTAACGTGAGCTACGACCCCACCCGCGAGTTCTACCGCGACTACAACACCGCTTTTGCCGCGCACTGGAAA
CAGCAGCACCCGCAGGACGCCGTCACCGTGGAAACCTCGCACGGCGGCTCGGGCAAGCAGGCGCGCGCGGTGATCGATGG
CATCGAGGCCGACGTGGTGACGTTGGCGCTGGCCTACGACGTGGATGCCATCGCGCAAAAGGCCAAGCTGATCGATACCG
ATTGGGAAGCGCGCCTGCCCGACAACAGTGCGCCGTACACCTCGACCATTGTGTTTCTGGTGCGCAAGGGCAACCCCAAG
AACATCCATGACTGGCCGGACCTGCTGCGCTCGGGCGTGTCGGTGGTGACCCCGAATCCGAAGACCTCCGGTGGCGCGCG
CTGGAACTATCTGGCCGCCTGGGCGTACGCCGACCATATCTTCAAGGGCGACCGCGAGCGCACCCTGCGCTACATGCAGG
CCTTGTTCCGCAATGTGCCGGTGCTGGATACCGGCGCGCGCGGCGCAACCACCACGTTCGTGCAGCGTGGGATCGGTGAT
GTGCTGCTGGCCTGGGAAAACGAGGCGCTGCTGGCGCGCGAGGAATTGGGCAAGGACAAGTTCGAGATCGTGGTACCGAA
GTTGTCGATCCTGGCCGAGCCATCGGTGGCGCTGGTCGACAAGAACGTCGACAAGCATGGCACCCGCGAGGTGGCCGAAG
CCTACCTGCGCTATCTGTACGCGCCGGAAGGCCAGAAGCTGGCAGCCAAGCATTTCTATCGCCCGCGGCATCCGGAATTT
GCCGACCCCGCCGACATGGCGCGTTTCCCGGATATCCAGCTGGTGACCATCCAGCAGGCGTTTGGCTCTTGGGACAAGGC
GCAACAGGAGCACTTCGCCGACGGTGGTGTGTTCGACCAGATCCAGGCCAACAAGTAG

Upstream 100 bases:

>100_bases
CGGTGGTCATGGCGGTCGCAGTGGCAGAAAGAAGCGCAATGTTAACGATCACGGTGGCATACCACTACGTCATACAAAAT
CGGCATAAGCGGCGGCGCTG

Downstream 100 bases:

>100_bases
GCGATGGCGATGTCGGTTGCACCCCACTCAGCGCCGTCATCGCGCCGACGCGTGATGCCGGGGCTGGGCCTGAGTTTGGG
TATCACCCTGACCTGGCTTG

Product: ABC transporter sulfate binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSFHRLRDAAQHGMLAVQLCSTGQEIVVTYPRIRLACLLCTLLVLAGPAGAREIGLLNVSYDPTREFYRDYNTAFAAHWK
QQHPQDAVTVETSHGGSGKQARAVIDGIEADVVTLALAYDVDAIAQKAKLIDTDWEARLPDNSAPYTSTIVFLVRKGNPK
NIHDWPDLLRSGVSVVTPNPKTSGGARWNYLAAWAYADHIFKGDRERTLRYMQALFRNVPVLDTGARGATTTFVQRGIGD
VLLAWENEALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLYAPEGQKLAAKHFYRPRHPEF
ADPADMARFPDIQLVTIQQAFGSWDKAQQEHFADGGVFDQIQANK

Sequences:

>Translated_365_residues
MSFHRLRDAAQHGMLAVQLCSTGQEIVVTYPRIRLACLLCTLLVLAGPAGAREIGLLNVSYDPTREFYRDYNTAFAAHWK
QQHPQDAVTVETSHGGSGKQARAVIDGIEADVVTLALAYDVDAIAQKAKLIDTDWEARLPDNSAPYTSTIVFLVRKGNPK
NIHDWPDLLRSGVSVVTPNPKTSGGARWNYLAAWAYADHIFKGDRERTLRYMQALFRNVPVLDTGARGATTTFVQRGIGD
VLLAWENEALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLYAPEGQKLAAKHFYRPRHPEF
ADPADMARFPDIQLVTIQQAFGSWDKAQQEHFADGGVFDQIQANK
>Mature_364_residues
SFHRLRDAAQHGMLAVQLCSTGQEIVVTYPRIRLACLLCTLLVLAGPAGAREIGLLNVSYDPTREFYRDYNTAFAAHWKQ
QHPQDAVTVETSHGGSGKQARAVIDGIEADVVTLALAYDVDAIAQKAKLIDTDWEARLPDNSAPYTSTIVFLVRKGNPKN
IHDWPDLLRSGVSVVTPNPKTSGGARWNYLAAWAYADHIFKGDRERTLRYMQALFRNVPVLDTGARGATTTFVQRGIGDV
LLAWENEALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLYAPEGQKLAAKHFYRPRHPEFA
DPADMARFPDIQLVTIQQAFGSWDKAQQEHFADGGVFDQIQANK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=322, Percent_Identity=67.3913043478261, Blast_Score=448, Evalue=1e-127,
Organism=Escherichia coli, GI1788765, Length=308, Percent_Identity=48.0519480519481, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40626; Mature: 40495

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFHRLRDAAQHGMLAVQLCSTGQEIVVTYPRIRLACLLCTLLVLAGPAGAREIGLLNVS
CCHHHHHHHHHCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YDPTREFYRDYNTAFAAHWKQQHPQDAVTVETSHGGSGKQARAVIDGIEADVVTLALAYD
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHEEEHHHHC
VDAIAQKAKLIDTDWEARLPDNSAPYTSTIVFLVRKGNPKNIHDWPDLLRSGVSVVTPNP
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCC
KTSGGARWNYLAAWAYADHIFKGDRERTLRYMQALFRNVPVLDTGARGATTTFVQRGIGD
CCCCCCCCHHEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCC
VLLAWENEALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLY
EEEEECCCHHHHHHHCCCCCEEEEECCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHC
APEGQKLAAKHFYRPRHPEFADPADMARFPDIQLVTIQQAFGSWDKAQQEHFADGGVFDQ
CCCCCHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEHHHHCCHHHHHHHHCCCCCCCCC
IQANK
CCCCC
>Mature Secondary Structure 
SFHRLRDAAQHGMLAVQLCSTGQEIVVTYPRIRLACLLCTLLVLAGPAGAREIGLLNVS
CHHHHHHHHHCCEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHCCCCCCCEEEEEECC
YDPTREFYRDYNTAFAAHWKQQHPQDAVTVETSHGGSGKQARAVIDGIEADVVTLALAYD
CCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCCHHHEEEHHHHC
VDAIAQKAKLIDTDWEARLPDNSAPYTSTIVFLVRKGNPKNIHDWPDLLRSGVSVVTPNP
HHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCHHHHHHHCCCEEECCCC
KTSGGARWNYLAAWAYADHIFKGDRERTLRYMQALFRNVPVLDTGARGATTTFVQRGIGD
CCCCCCCCHHEEHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCC
VLLAWENEALLAREELGKDKFEIVVPKLSILAEPSVALVDKNVDKHGTREVAEAYLRYLY
EEEEECCCHHHHHHHCCCCCEEEEECCHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHC
APEGQKLAAKHFYRPRHPEFADPADMARFPDIQLVTIQQAFGSWDKAQQEHFADGGVFDQ
CCCCCHHHHHHCCCCCCCCCCCCHHHHHCCCEEEEEEHHHHCCHHHHHHHHCCCCCCCCC
IQANK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]