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The map label for this gene is hisH [H]

Identifier: 84623779

GI number: 84623779

Start: 2348887

End: 2349489

Strand: Direct

Name: hisH [H]

Synonym: XOO_2122

Alternate gene names: 84623779

Gene position: 2348887-2349489 (Clockwise)

Preceding gene: 84623778

Following gene: 84623780

Centisome position: 47.55

GC content: 64.18

Gene sequence:

>603_bases
ATGACCGATGTTGCGCTGATCGATGCCGGTGGCGCCAATCTGGGCTCGGTGCGCTATGCATTGGAGCGACTGGGCGTTGA
GGCACGCGTGGTGCGCGATGCAGCCGGCTTGCAGGGCGCGCAGCGGGTGATACTGCCTGGTGTTGGCGCTGCGCCAGAGG
TGATGTCGCGTTTGCGCGCGCAGGGCTTGGTCGCGCCATTGCGCGAGCTGCAGGTGCCGTTGATCGGTATCTGCTTAGGG
ATGCAGTTGCTGTTCGAACATTCCGAAGAAGGCGATGTCGAGTGCCTGGGTCTGCTGCCGGGCATCGTGCGGCATATGAC
GCCTGCGCTTGGCATTCGTGTGCCGCATATGGGCTGGAATCGGCTGGTGCCGATGCGCGAATCCGCGTTGCTGGCCGGTT
TGCCGGAGCGCGCCAGTGCGTATTTCGTGCATGGTTACGCGGCGCCGGTCACGGCCGACACCGTGGCTGCCTGCGATCAC
GGCGGCCTGTTCACTGCCATCGTGCAGAACGGCTTGCGCTGCGGCGCGCAGTTTCACCCTGAGCGGTCGGCCGACACCGG
TGCACGCATCCTGCACAATTTTCTTGAGATGAGCTTCCCATGA

Upstream 100 bases:

>100_bases
ATCACCACAAGGTCGAGGCCTGCTTCAAGGCGTTGGCGCGTGCATTGCGTCAGGCGATTCGCCGTGAGGGCAGCGCATTG
CCCACCACCAAGGGCGCGCT

Downstream 100 bases:

>100_bases
GTTTCACCGTTTACCCGGCGCTGGATATTCGCAATGGCCGCGTGGTGCGCTTGCTGCAGGGCGATTACGCACGCGAAACG
CACTACGGCAACGACGTGCT

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 200; Mature: 199

Protein sequence:

>200_residues
MTDVALIDAGGANLGSVRYALERLGVEARVVRDAAGLQGAQRVILPGVGAAPEVMSRLRAQGLVAPLRELQVPLIGICLG
MQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRVPHMGWNRLVPMRESALLAGLPERASAYFVHGYAAPVTADTVAACDH
GGLFTAIVQNGLRCGAQFHPERSADTGARILHNFLEMSFP

Sequences:

>Translated_200_residues
MTDVALIDAGGANLGSVRYALERLGVEARVVRDAAGLQGAQRVILPGVGAAPEVMSRLRAQGLVAPLRELQVPLIGICLG
MQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRVPHMGWNRLVPMRESALLAGLPERASAYFVHGYAAPVTADTVAACDH
GGLFTAIVQNGLRCGAQFHPERSADTGARILHNFLEMSFP
>Mature_199_residues
TDVALIDAGGANLGSVRYALERLGVEARVVRDAAGLQGAQRVILPGVGAAPEVMSRLRAQGLVAPLRELQVPLIGICLGM
QLLFEHSEEGDVECLGLLPGIVRHMTPALGIRVPHMGWNRLVPMRESALLAGLPERASAYFVHGYAAPVTADTVAACDHG
GLFTAIVQNGLRCGAQFHPERSADTGARILHNFLEMSFP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=196, Percent_Identity=45.4081632653061, Blast_Score=164, Evalue=3e-42,
Organism=Saccharomyces cerevisiae, GI6319725, Length=214, Percent_Identity=30.8411214953271, Blast_Score=98, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 21249; Mature: 21118

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVALIDAGGANLGSVRYALERLGVEARVVRDAAGLQGAQRVILPGVGAAPEVMSRLRA
CCCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
QGLVAPLRELQVPLIGICLGMQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRVPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHCEECCCCCCC
RLVPMRESALLAGLPERASAYFVHGYAAPVTADTVAACDHGGLFTAIVQNGLRCGAQFHP
CCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
ERSADTGARILHNFLEMSFP
CCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDVALIDAGGANLGSVRYALERLGVEARVVRDAAGLQGAQRVILPGVGAAPEVMSRLRA
CCEEEEECCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEECCCCCCCHHHHHHHHH
QGLVAPLRELQVPLIGICLGMQLLFEHSEEGDVECLGLLPGIVRHMTPALGIRVPHMGWN
HHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCHHHCEECCCCCCC
RLVPMRESALLAGLPERASAYFVHGYAAPVTADTVAACDHGGLFTAIVQNGLRCGAQFHP
CCCCCCHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCCCCC
ERSADTGARILHNFLEMSFP
CCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]