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The map label for this gene is pyrB [H]

Identifier: 84622968

GI number: 84622968

Start: 1431339

End: 1432286

Strand: Direct

Name: pyrB [H]

Synonym: XOO_1311

Alternate gene names: 84622968

Gene position: 1431339-1432286 (Clockwise)

Preceding gene: 84622967

Following gene: 84622969

Centisome position: 28.97

GC content: 66.77

Gene sequence:

>948_bases
ATGACCACCATGCAACTCGATTCGGACGGACGCCTGCGCCACCTTCTCACCCTGGAAGGAGTGCCGCGTACCACGCTGCT
GCAATTGCTCGACCGCGCCGGCCAGATCCGCGATGCGGCGGTGGGGCGCGTGGGCAAGCGCAGCGTGCTGGCGGGCACCG
CGGTGTGCACGCTGTTCTTCGAACCCTCCACGCGCACGCGCAGTTCCTTCCATCTGGCGGCGCAGCGACTGGGTGCGGAC
GTGCTCAATTTCGATGCCTCCACCTCATCCACCCGCAAGGGCGAAACCGCGCGCGACACGCTGAAGAATCTCGAAGCGAT
GGGTGTGCGCGGCTTTGTGGTGCGTCACCCCGACGACGGCGCGGTGAAAGCGCTGGCCGCCGCAGCAGGCGAGGGCACCG
CGTTGATCAATGCCGGCGACGGACGCAGCGCGCATCCTACCCAGGGCCTGCTCGACATGCTCACCCTGCGCCAGGCCAAG
GGAACCGATTTTTCCAAGCTCAAGGTCGTCATCGTCGGCGACGTCAAGCACTCACGGGTGGCGCGCTCGGACCTGCATGC
CCTGCGCACGCTGGGCGCTGGCGAAATCCGCGTCTGCGGCCCGGCCAGCCTGCTACCTGACGACGGCATACTGGAGGGCT
GCGTAGTCGGTCAGGATTTCGATGCCATGCTCGAAGGCGCCGACGCGTTGATGATGCTGCGCCTGCAGCGCGAGCGTATG
GAAGAAGGCTTGGTGCCGTCGCTGGAGCAATATCACACCGAATACGGCCTGACCCGCGAGCGTCTTGCGCGCGCCGGCCA
TGACGCTGCGGTGCTGCATCCGGGCCCGATCAACCGCGGCGTGGAAATCACTGATGAAGTGGCCGACGGCGCGCAATCCT
GCGTGCTGCGGCAGGTCGCCAACGGCGTCGCCGTGCGCATGGCGGTACTGGAAACGCTGCTGGGCTAA

Upstream 100 bases:

>100_bases
GATGCCGAAGCGCTCGATGCCATGGCCGCTGCGGTGATCGTCGAACGCTGGTTGGCCGCGCCCGACCAAGCCACCCTTCT
TCCTTGACTCCCGACCTGCG

Downstream 100 bases:

>100_bases
GCGCAACTAACAACAACTGCGCTCACCGCCAGGCGGGCGCGGCCGGTGCTGGAATCGGCATGTGCCACTCGTACACTGCG
GCTTCTCCGCGTCGTCGGCA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MTTMQLDSDGRLRHLLTLEGVPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVKALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTRERLARAGHDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Sequences:

>Translated_315_residues
MTTMQLDSDGRLRHLLTLEGVPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGAD
VLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVKALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAK
GTDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
EEGLVPSLEQYHTEYGLTRERLARAGHDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG
>Mature_314_residues
TTMQLDSDGRLRHLLTLEGVPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFFEPSTRTRSSFHLAAQRLGADV
LNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDGAVKALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKG
TDFSKLKVVIVGDVKHSRVARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERME
EGLVPSLEQYHTEYGLTRERLARAGHDAAVLHPGPINRGVEITDEVADGAQSCVLRQVANGVAVRMAVLETLLG

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=310, Percent_Identity=34.5161290322581, Blast_Score=146, Evalue=2e-35,
Organism=Escherichia coli, GI2367364, Length=312, Percent_Identity=31.0897435897436, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786469, Length=340, Percent_Identity=25.8823529411765, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=293, Percent_Identity=32.7645051194539, Blast_Score=108, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6322331, Length=319, Percent_Identity=33.8557993730408, Blast_Score=142, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24642586, Length=277, Percent_Identity=36.4620938628159, Blast_Score=143, Evalue=2e-34,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082 [H]

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N [H]

EC number: =2.1.3.2 [H]

Molecular weight: Translated: 33603; Mature: 33472

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTMQLDSDGRLRHLLTLEGVPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CCEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVKALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTRERLARAGHDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC
>Mature Secondary Structure 
TTMQLDSDGRLRHLLTLEGVPRTTLLQLLDRAGQIRDAAVGRVGKRSVLAGTAVCTLFF
CEEEECCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHEEEEEEEE
EPSTRTRSSFHLAAQRLGADVLNFDASTSSTRKGETARDTLKNLEAMGVRGFVVRHPDDG
CCCCCCHHHHHHHHHHHCCHHEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCH
AVKALAAAAGEGTALINAGDGRSAHPTQGLLDMLTLRQAKGTDFSKLKVVIVGDVKHSRV
HHHHHHHHCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEEEECCHHHHH
ARSDLHALRTLGAGEIRVCGPASLLPDDGILEGCVVGQDFDAMLEGADALMMLRLQRERM
HHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCEEECCCHHHHHHCHHHHHHHHHHHHHH
EEGLVPSLEQYHTEYGLTRERLARAGHDAAVLHPGPINRGVEITDEVADGAQSCVLRQVA
HCCCCCCHHHHHHHHCCCHHHHHHCCCCCEEECCCCCCCCCEEHHHHHHHHHHHHHHHHH
NGVAVRMAVLETLLG
HHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA