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The map label for this gene is dctA [H]

Identifier: 84622671

GI number: 84622671

Start: 1110451

End: 1111824

Strand: Direct

Name: dctA [H]

Synonym: XOO_1014

Alternate gene names: 84622671

Gene position: 1110451-1111824 (Clockwise)

Preceding gene: 84622666

Following gene: 84622672

Centisome position: 22.48

GC content: 63.68

Gene sequence:

>1374_bases
ATGCACATCAGCAAACCTGCCGGCCCCCTGCCGGCATCTGTCCCCTTCTATCGCCAGCTGTATTTCCAGGTGGTGGTGGC
GATCATCCTGGGTGCCCTGCTGGGCCGTTTCGAACCCGCCTTTGCCGAAAGCCTCAAGCCGCTGGGCGACGCCTTCATCA
AGCTGGTGAAGATGATCATCGCCCCGGTGATCTTCCTGACCATCGTGACCGGCATCGCCGGCATGACCCACCTAAAAACG
GTGGGCCGGGTGTTCGGCAAGGCGATGGTCTACTTTCTGTTCTTCTCCACCTTGGCCCTGGTGGTCGGTCTGGTCGTTGC
CCATGTGGTGCAGCCCGGCGCCGGCATGAACATCAACCCGGCCGATCTGGATCAGAGCGCGGTCAAGAGCTATGTCGAGA
AGTCGCACGACCTGACCCTGGTCGGCTTCCTGATGGACATCATCCCCAACTCGCTGATCGGCGCCTTCACCGGCGACCAG
GTGGTCAACGGCAAGCTGACCGGGCCGAACATCTTGCAGGTGCTGTTCGTGGCGGTGCTGTTCGGTGTGTCGCTGGCGTT
GGTGGGCGAGCGCGGCAAGCCGGTGCTGAACCTGCTGGAAGCGCTGATCGCCCCGGTGTTCAAGCTGGTGCACATCCTGA
TGCGTGCTGCGCCGATCGGTGCCTTCGGTGCGATCGCCTTCACCATCGGCAAGTACGGCGTCGAGTCGTTGGTCAATCTG
GCCTGGCTGGTGGGGTCGTTTTACCTGACCTCCTTGTTGTTCGTGCTGGTGATCCTGGGGGTGGTGTGTCGGCTGTGCGG
GTTTTCGGTACTCAAGCTGATTCGCTATCTGAAAGCCGAACTGCTGCTGGTGCTGGGCACGTCCTCGTCGGAGTCGGCAT
TGCCGTCGCTGATGGAAAAGATGGAAAAGGCCGGTTGCGAGAAGTCGGTGGTGGGCCTGGTGGTGCCGACCGGGTACTCG
TTCAATCTGGACGGCACCAACATCTATATGACGCTGGCGGCGCTGTTCATCGCCCAGGCGACCAATACCGAGCTGACCCT
GGGCCACCAGATCGCACTGCTGGCCGTGGCAATGCTCAGCTCCAAGGGCGCAGCCGGCGTCACCGGTGCGGGCTTCATCA
CCCTGGCCGCCACGCTGGCGGTGGTGCCTGAGGTGCCGGTGGCCGGCATGGCGCTGATCCTGGGCGTGGACCGTTTCATG
AGCGAATGCCGCTCGTTGACCAACTTCATCGGCAATGCGGTGGCCACCGTGGTGGTGTCGCGCTGGGAAAATGCATTGGA
CCGCGACCGCCTCACGCTGGTCCTGGACGGCGGCGAACCGCCGCTGCTGGCCCCGGTCGGCGAGCCCGGCGTGGCGCCTG
CCGCCCTGCGTTGA

Upstream 100 bases:

>100_bases
GTAGTACCAATGGGCTATCGGCCAGGGCTGTGACGTACGGCCAGAATGCGTGCAACGGGTACCTGCTCAGGCACCCCCTC
TCGTCCCTCGGAGTGGTGTC

Downstream 100 bases:

>100_bases
TGCTTCCTGCGGCCCTGTCTTCCGTCGGTATTGCCGGCGGGATGCAGGGCCGTTTTCGCGTTGAGCCACACTCCGCGCCG
CGCACGCCGCGTTGCAGCAT

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIILGALLGRFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMVYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGVVCRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLTLVLDGGEPPLLAPVGEPGVAPAALR

Sequences:

>Translated_457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIILGALLGRFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMVYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGVVCRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLTLVLDGGEPPLLAPVGEPGVAPAALR
>Mature_457_residues
MHISKPAGPLPASVPFYRQLYFQVVVAIILGALLGRFEPAFAESLKPLGDAFIKLVKMIIAPVIFLTIVTGIAGMTHLKT
VGRVFGKAMVYFLFFSTLALVVGLVVAHVVQPGAGMNINPADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQ
VVNGKLTGPNILQVLFVAVLFGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
AWLVGSFYLTSLLFVLVILGVVCRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEKMEKAGCEKSVVGLVVPTGYS
FNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLSSKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFM
SECRSLTNFIGNAVATVVVSRWENALDRDRLTLVLDGGEPPLLAPVGEPGVAPAALR

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI4827012, Length=448, Percent_Identity=25.6696428571429, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI169790839, Length=455, Percent_Identity=26.8131868131868, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI194239697, Length=458, Percent_Identity=25.5458515283843, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI21314632, Length=397, Percent_Identity=26.9521410579345, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI40254478, Length=448, Percent_Identity=25.8928571428571, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI66773030, Length=396, Percent_Identity=23.989898989899, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI5032093, Length=320, Percent_Identity=28.75, Blast_Score=96, Evalue=9e-20,
Organism=Homo sapiens, GI262359914, Length=398, Percent_Identity=25.6281407035176, Blast_Score=94, Evalue=3e-19,
Organism=Homo sapiens, GI223468566, Length=283, Percent_Identity=30.7420494699647, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI223468564, Length=267, Percent_Identity=30.7116104868914, Blast_Score=90, Evalue=4e-18,
Organism=Homo sapiens, GI301601644, Length=168, Percent_Identity=35.7142857142857, Blast_Score=81, Evalue=3e-15,
Organism=Escherichia coli, GI1789947, Length=424, Percent_Identity=57.7830188679245, Blast_Score=501, Evalue=1e-143,
Organism=Escherichia coli, GI1790514, Length=424, Percent_Identity=39.3867924528302, Blast_Score=301, Evalue=7e-83,
Organism=Escherichia coli, GI1788024, Length=398, Percent_Identity=24.6231155778894, Blast_Score=80, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17537407, Length=422, Percent_Identity=28.1990521327014, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71996953, Length=448, Percent_Identity=28.3482142857143, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983099, Length=458, Percent_Identity=26.6375545851528, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983106, Length=458, Percent_Identity=26.6375545851528, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI193206505, Length=417, Percent_Identity=23.5011990407674, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI193206654, Length=476, Percent_Identity=22.2689075630252, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17541374, Length=399, Percent_Identity=22.8070175438596, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24583025, Length=441, Percent_Identity=24.2630385487528, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17137668, Length=441, Percent_Identity=24.2630385487528, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI24583023, Length=441, Percent_Identity=24.2630385487528, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI281360483, Length=456, Percent_Identity=24.1228070175439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137666, Length=456, Percent_Identity=24.1228070175439, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360481, Length=456, Percent_Identity=24.1228070175439, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 48129; Mature: 48129

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHISKPAGPLPASVPFYRQLYFQVVVAIILGALLGRFEPAFAESLKPLGDAFIKLVKMII
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
APVIFLTIVTGIAGMTHLKTVGRVFGKAMVYFLFFSTLALVVGLVVAHVVQPGAGMNINP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQVVNGKLTGPNILQVLFVAVL
HHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHH
FGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AWLVGSFYLTSLLFVLVILGVVCRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
MEKAGCEKSVVGLVVPTGYSFNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLS
HHHCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
SKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFMSECRSLTNFIGNAVATVVVS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWENALDRDRLTLVLDGGEPPLLAPVGEPGVAPAALR
HHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure
MHISKPAGPLPASVPFYRQLYFQVVVAIILGALLGRFEPAFAESLKPLGDAFIKLVKMII
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
APVIFLTIVTGIAGMTHLKTVGRVFGKAMVYFLFFSTLALVVGLVVAHVVQPGAGMNINP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
ADLDQSAVKSYVEKSHDLTLVGFLMDIIPNSLIGAFTGDQVVNGKLTGPNILQVLFVAVL
HHCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHHHHHHHH
FGVSLALVGERGKPVLNLLEALIAPVFKLVHILMRAAPIGAFGAIAFTIGKYGVESLVNL
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AWLVGSFYLTSLLFVLVILGVVCRLCGFSVLKLIRYLKAELLLVLGTSSSESALPSLMEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHHH
MEKAGCEKSVVGLVVPTGYSFNLDGTNIYMTLAALFIAQATNTELTLGHQIALLAVAMLS
HHHCCCCCCEEEEEEECCEEECCCCCHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHH
SKGAAGVTGAGFITLAATLAVVPEVPVAGMALILGVDRFMSECRSLTNFIGNAVATVVVS
CCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWENALDRDRLTLVLDGGEPPLLAPVGEPGVAPAALR
HHHHHHCCCCEEEEEECCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]