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The map label for this gene is hrcU [H]

Identifier: 84621748

GI number: 84621748

Start: 96337

End: 97416

Strand: Direct

Name: hrcU [H]

Synonym: XOO_0091

Alternate gene names: 84621748

Gene position: 96337-97416 (Clockwise)

Preceding gene: 84621738

Following gene: 84621749

Centisome position: 1.95

GC content: 58.89

Gene sequence:

>1080_bases
ATGTCGGAAGAAAAAACCGAGAAGCCGACCGAGAAGAAACTGCGGGATGCGCGCAAGGACGGGGAAGTTCCGGTCAGCCC
GGACGTCACTGCAGCCGCGGTGCTCTTCGGCGCGTTATTGGTGATGAAATCGGCCGGCGATTACTTCGTCGATCACATGC
GCGCATTGACGAGGATCGGCTTCGATTTTTCGGAAAACACACGCGATGCAACGGCGATAAACCGTGCACTCGCCCATATC
GGCATCCAGGGATTGCTGTTGATGCTGCCGTTCCTGGCTGCGTGCCTGGTGGCTGGCTTGGTAGGCGGTGCGTTTCAGAC
GGGCCTGAACGCCTCGCTCAAGCCGGTGTCGCCCAAGTTCGATTCGCTCAATCCCGCAAACGGGGTCAAGAAACTTTTTT
CGTTGAGAAGTCTCATCAATCTACTGAAGTTGATCATCAAGGCCATCCTGATTGGCGTGGTGATGTGGGTCGGTATCCGC
ACCCTCATGCCGATGATCATTGGGCTTGCCTACGAGACACCGCTTGACATTTCCCAAATTGCCTGGCGAACCCTGAGCAT
GCTCTTCGCACTGGGCGTGCTGCTGTTCATACTGGTCGGCGCTGCAGACTGGAGCGTGCAGCACTGGCTGTTCATCCGCG
ACAAGCGAATGAGCAAGGACGAGCAGAAGCGCGAATACAAGGAAAGCGAGGGCGACCCCGAAATCAAGGGCAAACGCAAG
GAATTCGCCAAGGAACTGGTCTTCGGCGACCCCCGCCAACGCGTCGCCAAGGCCAAGGTGATGGTGGTCAACCCGACCCA
TTACGCGGTGGCATTGGCCTACGAGCCCGATGACTTCGGCTTGCCGCAGGTCGTTGCCAAGGGCGTAGACGATGGCGCGC
TGGAACTGCGCGCCTTTGCGCATAACCAGGGCATTCCGATCGTTGCCAATCCGCCACTGGCACGTGCGCTGTATCAGGTG
GAATTGGGCGACGCAATCCCTGAGCAATTGTTCGAAACGGTTGCGGTGGTGTTGCGCTGGGTCGATGGACTCGGTCGCGA
CCACGGCGACGGCGACGGCAATGGAGCCCTGCCATGCTAG

Upstream 100 bases:

>100_bases
TCCGGCTTCGCCAGGCCATCCACACATTCGCCTGGAAAACGAATCGCTCGACCACAGCTCCCCACAATCGGGGGCGCTGC
GATCGCGGAAGGATAAGGCA

Downstream 100 bases:

>100_bases
GAGATCGCGTGCGAGCGACAAGGTATTTCGCTTATAGCGGCGAAGTGGCGATCGCCGCCTTGGTGGTGGCGGTCATCGGG
CTGATGATCCTGCCCCTGCC

Product: type III secretion system protein HrcU

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 359; Mature: 358

Protein sequence:

>359_residues
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFVDHMRALTRIGFDFSENTRDATAINRALAHI
GIQGLLLMLPFLAACLVAGLVGGAFQTGLNASLKPVSPKFDSLNPANGVKKLFSLRSLINLLKLIIKAILIGVVMWVGIR
TLMPMIIGLAYETPLDISQIAWRTLSMLFALGVLLFILVGAADWSVQHWLFIRDKRMSKDEQKREYKESEGDPEIKGKRK
EFAKELVFGDPRQRVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQV
ELGDAIPEQLFETVAVVLRWVDGLGRDHGDGDGNGALPC

Sequences:

>Translated_359_residues
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFVDHMRALTRIGFDFSENTRDATAINRALAHI
GIQGLLLMLPFLAACLVAGLVGGAFQTGLNASLKPVSPKFDSLNPANGVKKLFSLRSLINLLKLIIKAILIGVVMWVGIR
TLMPMIIGLAYETPLDISQIAWRTLSMLFALGVLLFILVGAADWSVQHWLFIRDKRMSKDEQKREYKESEGDPEIKGKRK
EFAKELVFGDPRQRVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQV
ELGDAIPEQLFETVAVVLRWVDGLGRDHGDGDGNGALPC
>Mature_358_residues
SEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFVDHMRALTRIGFDFSENTRDATAINRALAHIG
IQGLLLMLPFLAACLVAGLVGGAFQTGLNASLKPVSPKFDSLNPANGVKKLFSLRSLINLLKLIIKAILIGVVMWVGIRT
LMPMIIGLAYETPLDISQIAWRTLSMLFALGVLLFILVGAADWSVQHWLFIRDKRMSKDEQKREYKESEGDPEIKGKRKE
FAKELVFGDPRQRVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFAHNQGIPIVANPPLARALYQVE
LGDAIPEQLFETVAVVLRWVDGLGRDHGDGDGNGALPC

Specific function: Involved in the secretion of popA, a proteinaceous elicitor of the hypersensitivity response in plants [H]

COG id: COG1377

COG function: function code NU; Flagellar biosynthesis pathway, component FlhB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type III secretion exporter family [H]

Homologues:

Organism=Escherichia coli, GI1788188, Length=347, Percent_Identity=33.4293948126801, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006135
- InterPro:   IPR006307 [H]

Pfam domain/function: PF01312 Bac_export_2 [H]

EC number: NA

Molecular weight: Translated: 39252; Mature: 39121

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFVDHMRALTRIG
CCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
FDFSENTRDATAINRALAHIGIQGLLLMLPFLAACLVAGLVGGAFQTGLNASLKPVSPKF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DSLNPANGVKKLFSLRSLINLLKLIIKAILIGVVMWVGIRTLMPMIIGLAYETPLDISQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AWRTLSMLFALGVLLFILVGAADWSVQHWLFIRDKRMSKDEQKREYKESEGDPEIKGKRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHHCCCHHHHHHHHHCCCCCCCCCHHH
EFAKELVFGDPRQRVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFA
HHHHHHHCCCHHHHHHHCEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHH
HNQGIPIVANPPLARALYQVELGDAIPEQLFETVAVVLRWVDGLGRDHGDGDGNGALPC
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SEEKTEKPTEKKLRDARKDGEVPVSPDVTAAAVLFGALLVMKSAGDYFVDHMRALTRIG
CCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHC
FDFSENTRDATAINRALAHIGIQGLLLMLPFLAACLVAGLVGGAFQTGLNASLKPVSPKF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DSLNPANGVKKLFSLRSLINLLKLIIKAILIGVVMWVGIRTLMPMIIGLAYETPLDISQI
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
AWRTLSMLFALGVLLFILVGAADWSVQHWLFIRDKRMSKDEQKREYKESEGDPEIKGKRK
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHEEEEHHHCCCHHHHHHHHHCCCCCCCCCHHH
EFAKELVFGDPRQRVAKAKVMVVNPTHYAVALAYEPDDFGLPQVVAKGVDDGALELRAFA
HHHHHHHCCCHHHHHHHCEEEEECCCCEEEEEEECCCCCCCHHHHHCCCCCCHHHHHHHH
HNQGIPIVANPPLARALYQVELGDAIPEQLFETVAVVLRWVDGLGRDHGDGDGNGALPC
CCCCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8316211; 11823852 [H]