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The map label for this gene is argF

Identifier: 84488861

GI number: 84488861

Start: 33553

End: 34461

Strand: Direct

Name: argF

Synonym: Msp_0031

Alternate gene names: 84488861

Gene position: 33553-34461 (Clockwise)

Preceding gene: 84488860

Following gene: 84488862

Centisome position: 1.9

GC content: 29.7

Gene sequence:

>909_bases
ATGAAAAACTTATTATCAATGTCTGATGCAAAAAATGAAGTAAATGCAATATTAGAAATAGCATCAGGTTTAAAATCAGG
TGAAATAACTGAAAAACCACTAACAAACAAATATATTGGAATGATTTTTGAAAAATCATCAACAAGAACAAGAGTATCAT
TTGAAGTTGGAATTCATCAACTAGGTGGAACTCCATTGTATCTTTCAAGTAAAGACCTACAATTAAATAGGGGAGAACCA
ATAGAAGATACTGCAAGGGTATTATCACGTTTTCTTGATGGAATAATGATAAGAGCAAAAAAACATGAAAATGTAGAAGA
ATTAGTACAATACTCCACAATACCTATTATAAGTGGATTAACAGATAAAGAACATCCATGTCAAATTTTTGCTGATTTAT
TAACAATAAAAGAATATAAAGGCGATTTTAACGATAAAAAAATCGTGTTTATAGGTGATGGAAACAATGTATGTAACTCT
TTATTACTAGCAGCAGCCTATGTTGGAATGGATATGACAGTAGCTTGTCCTGAAGGCTATGAACCAAATGAAAAAATATA
TACACTTGCAAAACAAGAAGCTCAAAAAACTAATTCAACAATAAAAATTGAAAACAATGTAAATGAAGCAGTAAAAGATG
CAGATGTATTATATACTGATGTATGGGTAAGTATGGGTGATGAAGATGAACAAGATGAAAGAGAAAGAATCTTCAAACCA
TATCAAATTAACAATGAATTATTATCACAAGCAAAAGATGATGCAATAGTAATGCACTGTTTACCTGCAATAAGAGGTCA
AGAAATAACAGCAGATGTAATGGTATCACCTCAATCAGCAATATGGGATCAAGCTGAAAATAGATTACATGCACAAAAAG
CAATACTATATCATATTTTTAAAGAATAA

Upstream 100 bases:

>100_bases
TCTATTTTTAAGTTACTTATTTTAAGAACTAGTAACATAATAATATATTATTTATTATTAAAATTATGAATTAATAATTT
TTTTAGGGAAGGATTCGAAA

Downstream 100 bases:

>100_bases
AAGAATTTACCTTCCCATAAAATCTTTTATTAATCATCATCCAAAGTTATATATTTATTTTTTAATTATGAAGTGATAAT
ATGTTATCAAAAAGAATCAT

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MKNLLSMSDAKNEVNAILEIASGLKSGEITEKPLTNKYIGMIFEKSSTRTRVSFEVGIHQLGGTPLYLSSKDLQLNRGEP
IEDTARVLSRFLDGIMIRAKKHENVEELVQYSTIPIISGLTDKEHPCQIFADLLTIKEYKGDFNDKKIVFIGDGNNVCNS
LLLAAAYVGMDMTVACPEGYEPNEKIYTLAKQEAQKTNSTIKIENNVNEAVKDADVLYTDVWVSMGDEDEQDERERIFKP
YQINNELLSQAKDDAIVMHCLPAIRGQEITADVMVSPQSAIWDQAENRLHAQKAILYHIFKE

Sequences:

>Translated_302_residues
MKNLLSMSDAKNEVNAILEIASGLKSGEITEKPLTNKYIGMIFEKSSTRTRVSFEVGIHQLGGTPLYLSSKDLQLNRGEP
IEDTARVLSRFLDGIMIRAKKHENVEELVQYSTIPIISGLTDKEHPCQIFADLLTIKEYKGDFNDKKIVFIGDGNNVCNS
LLLAAAYVGMDMTVACPEGYEPNEKIYTLAKQEAQKTNSTIKIENNVNEAVKDADVLYTDVWVSMGDEDEQDERERIFKP
YQINNELLSQAKDDAIVMHCLPAIRGQEITADVMVSPQSAIWDQAENRLHAQKAILYHIFKE
>Mature_302_residues
MKNLLSMSDAKNEVNAILEIASGLKSGEITEKPLTNKYIGMIFEKSSTRTRVSFEVGIHQLGGTPLYLSSKDLQLNRGEP
IEDTARVLSRFLDGIMIRAKKHENVEELVQYSTIPIISGLTDKEHPCQIFADLLTIKEYKGDFNDKKIVFIGDGNNVCNS
LLLAAAYVGMDMTVACPEGYEPNEKIYTLAKQEAQKTNSTIKIENNVNEAVKDADVLYTDVWVSMGDEDEQDERERIFKP
YQINNELLSQAKDDAIVMHCLPAIRGQEITADVMVSPQSAIWDQAENRLHAQKAILYHIFKE

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=255, Percent_Identity=40.7843137254902, Blast_Score=218, Evalue=4e-57,
Organism=Homo sapiens, GI18105007, Length=272, Percent_Identity=27.9411764705882, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1790703, Length=322, Percent_Identity=37.888198757764, Blast_Score=215, Evalue=3e-57,
Organism=Escherichia coli, GI1786469, Length=328, Percent_Identity=37.1951219512195, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=26.9736842105263, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994908, Length=309, Percent_Identity=25.5663430420712, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI193204318, Length=320, Percent_Identity=27.1875, Blast_Score=88, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6322373, Length=267, Percent_Identity=36.7041198501873, Blast_Score=179, Evalue=6e-46,
Organism=Saccharomyces cerevisiae, GI6322331, Length=303, Percent_Identity=28.0528052805281, Blast_Score=96, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24642586, Length=293, Percent_Identity=26.2798634812287, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_METST (Q2NI83)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447093.1
- HSSP:   P96108
- ProteinModelPortal:   Q2NI83
- SMR:   Q2NI83
- STRING:   Q2NI83
- GeneID:   3855039
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0031
- NMPDR:   fig|339860.6.peg.30
- eggNOG:   arNOG04256
- HOGENOM:   HBG579429
- OMA:   CVVTDTW
- PhylomeDB:   Q2NI83
- ProtClustDB:   PRK00779
- BioCyc:   MSTA339860:MSP_0031-MONOMER
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 33947; Mature: 33947

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 98-98 BINDING 125-125

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNLLSMSDAKNEVNAILEIASGLKSGEITEKPLTNKYIGMIFEKSSTRTRVSFEVGIHQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEECHHH
LGGTPLYLSSKDLQLNRGEPIEDTARVLSRFLDGIMIRAKKHENVEELVQYSTIPIISGL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCHHCCCC
TDKEHPCQIFADLLTIKEYKGDFNDKKIVFIGDGNNVCNSLLLAAAYVGMDMTVACPEGY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCC
EPNEKIYTLAKQEAQKTNSTIKIENNVNEAVKDADVLYTDVWVSMGDEDEQDERERIFKP
CCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHCCCC
YQINNELLSQAKDDAIVMHCLPAIRGQEITADVMVSPQSAIWDQAENRLHAQKAILYHIF
EECCHHHHHHCCCCEEEEHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KE
CC
>Mature Secondary Structure
MKNLLSMSDAKNEVNAILEIASGLKSGEITEKPLTNKYIGMIFEKSSTRTRVSFEVGIHQ
CCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHEEEEEECCCCCEEEEEEECHHH
LGGTPLYLSSKDLQLNRGEPIEDTARVLSRFLDGIMIRAKKHENVEELVQYSTIPIISGL
CCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHEEEECCCCCHHHHHHHHCCHHCCCC
TDKEHPCQIFADLLTIKEYKGDFNDKKIVFIGDGNNVCNSLLLAAAYVGMDMTVACPEGY
CCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHHHHHHHHCCCEEEECCCCC
EPNEKIYTLAKQEAQKTNSTIKIENNVNEAVKDADVLYTDVWVSMGDEDEQDERERIFKP
CCCHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHEEEEEEEECCCCCCHHHHHHCCCC
YQINNELLSQAKDDAIVMHCLPAIRGQEITADVMVSPQSAIWDQAENRLHAQKAILYHIF
EECCHHHHHHCCCCEEEEHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHH
KE
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA