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The map label for this gene is hisF

Identifier: 84488862

GI number: 84488862

Start: 34542

End: 35366

Strand: Direct

Name: hisF

Synonym: Msp_0032

Alternate gene names: 84488862

Gene position: 34542-35366 (Clockwise)

Preceding gene: 84488861

Following gene: 84488863

Centisome position: 1.95

GC content: 33.7

Gene sequence:

>825_bases
ATGTTATCAAAAAGAATCATACCATGTTTAGACTGTGATCTACAAGTACCTGAGGGTAGAGTAGTTAAAGGAGTAGAATT
TAAACAAATACGTTATGCTGGAAATCCAGTTGAATTAGCAACAAAATACTATGAACAAGGGGCAGATGAAATAGTATTTT
TAGATATAACCGCTTCCCATGAACGTAGATCTACAATGGCTGACGTAATTGAAAAAACAGTTGAAAATGTATTCACACCA
ATCTGTGTAGGTGGAGGAATACGAGAAGTAAAAGACTATGTGGCAATGCTTAAAGCAGGAGCAGATAAATGTTCAACAAA
TACTGCTGCAATAAAAGATCCATCCCTTATAAACAGAGCATCTGAGCATGTTGGAAGTCAAGCATGTGTAATTGGTATTG
ATGCTAAAAGAAGATATGTTGAAAATCCATCAGAATCTGATGAACATTATATTGTAGAAACAAATGATGGATATTGTTGG
TTTGACTGTAGTATTTATGGTGGACGTGAATTTACTGGAATTGATGCAGTTAAATGGGCAATAGAATGTGAAGAAAGAGG
TGCTGGAGAAATACTCTTAACAAGTATGGATAGGGATGGTACAAAGATAGGTTATGATTTAGAACTGACAAAAACAATAA
GTGAAAATGTTTCCATACCTGTAATAGCATCTGGTGGTGTGGGAAATCCTGAACATATCTATGATTCATTTTCTAAAGGA
AAAGCTGATGCAGCATTAGCAGCAAGTATATTTCACTTTGATGAATATCCAATACCTCAAGTAAAAAACTACTTAAAAGA
TAAAAATATTCCAATTAGAATATAA

Upstream 100 bases:

>100_bases
ATCATATTTTTAAAGAATAAAAGAATTTACCTTCCCATAAAATCTTTTATTAATCATCATCCAAAGTTATATATTTATTT
TTTAATTATGAAGTGATAAT

Downstream 100 bases:

>100_bases
TTTAATATTTTTACTTTCTACTTTTTTTAAGAATATTTAATATCACTTCTAACATATTAATAATTAAATAAATAGAATAC
TTATGGTTATCATGACATTT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MLSKRIIPCLDCDLQVPEGRVVKGVEFKQIRYAGNPVELATKYYEQGADEIVFLDITASHERRSTMADVIEKTVENVFTP
ICVGGGIREVKDYVAMLKAGADKCSTNTAAIKDPSLINRASEHVGSQACVIGIDAKRRYVENPSESDEHYIVETNDGYCW
FDCSIYGGREFTGIDAVKWAIECEERGAGEILLTSMDRDGTKIGYDLELTKTISENVSIPVIASGGVGNPEHIYDSFSKG
KADAALAASIFHFDEYPIPQVKNYLKDKNIPIRI

Sequences:

>Translated_274_residues
MLSKRIIPCLDCDLQVPEGRVVKGVEFKQIRYAGNPVELATKYYEQGADEIVFLDITASHERRSTMADVIEKTVENVFTP
ICVGGGIREVKDYVAMLKAGADKCSTNTAAIKDPSLINRASEHVGSQACVIGIDAKRRYVENPSESDEHYIVETNDGYCW
FDCSIYGGREFTGIDAVKWAIECEERGAGEILLTSMDRDGTKIGYDLELTKTISENVSIPVIASGGVGNPEHIYDSFSKG
KADAALAASIFHFDEYPIPQVKNYLKDKNIPIRI
>Mature_274_residues
MLSKRIIPCLDCDLQVPEGRVVKGVEFKQIRYAGNPVELATKYYEQGADEIVFLDITASHERRSTMADVIEKTVENVFTP
ICVGGGIREVKDYVAMLKAGADKCSTNTAAIKDPSLINRASEHVGSQACVIGIDAKRRYVENPSESDEHYIVETNDGYCW
FDCSIYGGREFTGIDAVKWAIECEERGAGEILLTSMDRDGTKIGYDLELTKTISENVSIPVIASGGVGNPEHIYDSFSKG
KADAALAASIFHFDEYPIPQVKNYLKDKNIPIRI

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=278, Percent_Identity=41.0071942446043, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI87082028, Length=232, Percent_Identity=24.5689655172414, Blast_Score=69, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319725, Length=314, Percent_Identity=38.8535031847134, Blast_Score=196, Evalue=2e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_METST (Q2NI84)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447094.1
- ProteinModelPortal:   Q2NI84
- SMR:   Q2NI84
- STRING:   Q2NI84
- GeneID:   3855040
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0032
- NMPDR:   fig|339860.6.peg.31
- eggNOG:   arNOG04606
- HOGENOM:   HBG541613
- OMA:   RVVKGTN
- PhylomeDB:   Q2NI84
- ProtClustDB:   PRK02083
- BioCyc:   MSTA339860:MSP_0032-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 30243; Mature: 30243

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSKRIIPCLDCDLQVPEGRVVKGVEFKQIRYAGNPVELATKYYEQGADEIVFLDITASH
CCCCCCCCEECCCCCCCCCCEEECCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
ERRSTMADVIEKTVENVFTPICVGGGIREVKDYVAMLKAGADKCSTNTAAIKDPSLINRA
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
SEHVGSQACVIGIDAKRRYVENPSESDEHYIVETNDGYCWFDCSIYGGREFTGIDAVKWA
HHHCCCCEEEEEECCHHHHCCCCCCCCCEEEEECCCCEEEEEEEEECCCEECCCHHEEEE
IECEERGAGEILLTSMDRDGTKIGYDLELTKTISENVSIPVIASGGVGNPEHIYDSFSKG
EEECCCCCCEEEEEECCCCCCEEEEEEEEEEHHHCCCCEEEEECCCCCCHHHHHHHHHCC
KADAALAASIFHFDEYPIPQVKNYLKDKNIPIRI
CCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEC
>Mature Secondary Structure
MLSKRIIPCLDCDLQVPEGRVVKGVEFKQIRYAGNPVELATKYYEQGADEIVFLDITASH
CCCCCCCCEECCCCCCCCCCEEECCEEEEEEECCCHHHHHHHHHHCCCCEEEEEEEECCC
ERRSTMADVIEKTVENVFTPICVGGGIREVKDYVAMLKAGADKCSTNTAAIKDPSLINRA
HHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHH
SEHVGSQACVIGIDAKRRYVENPSESDEHYIVETNDGYCWFDCSIYGGREFTGIDAVKWA
HHHCCCCEEEEEECCHHHHCCCCCCCCCEEEEECCCCEEEEEEEEECCCEECCCHHEEEE
IECEERGAGEILLTSMDRDGTKIGYDLELTKTISENVSIPVIASGGVGNPEHIYDSFSKG
EEECCCCCCEEEEEECCCCCCEEEEEEEEEEHHHCCCCEEEEECCCCCCHHHHHHHHHCC
KADAALAASIFHFDEYPIPQVKNYLKDKNIPIRI
CCCHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA