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The map label for this gene is purD

Identifier: 84488860

GI number: 84488860

Start: 32023

End: 33339

Strand: Direct

Name: purD

Synonym: Msp_0030

Alternate gene names: 84488860

Gene position: 32023-33339 (Clockwise)

Preceding gene: 84488857

Following gene: 84488861

Centisome position: 1.81

GC content: 30.68

Gene sequence:

>1317_bases
ATGAAAATATTGATGGTAGGTAGCGGTGCACGTGAACATGCAATTGCTAAGGCATTAAATAAAACAGCAGATGTTTATAC
ATATATGGGAAGAAAAAATCCTGGATTGGCAAGAATATCTAAAAATTATACAGTAAATGATGAATCTAATTTTAATGAAA
TTATTAAATTTGCACAAGAAAATAATGTGGAATTAGCATTTATTGGACCGGAAGCTCCACTTGAAATGGGAATAGTTGAT
GAACTTGAAAAAGAAGGAATACATTCTGTGGGTCCAACTAAAAGTGCAGCACAAATAGAAACAAACAAAGCATTCATGAG
AAAACTCTTTGAAGATTATGATATTCCAGGATCAATTAAATATGGAACATTTTATGATTTAGATGAAGCTTATGAATTTA
TTGATAACTTTGATGAACCAGTAGTAGTAAAACCAATAGGATTAACTGGTGGAAAAGGAGTTAAAATTGTAGGGGATCAG
TTAGCTGATAATGATGAAGCAAAAGAATATGTTAAAGAAATTTTCCATCAGAAAATGGGTGGATTTGAAGGAGTAGTAAT
TGAAGAATTGTTACTTGGAGAAGAATACACAATACAAGCTTTTGTTGATGGAACACATCTTATACCAATGCCTGCTGCAC
AAGATCATCCACATGCATTTGTAGGAAATAAAGGACCAATTACTGGGGGAATGGGTTCATATTCAGATAAAAATCATTTA
CTACCATTTTTAACACAAGAAGATTATGATAAATCAGTAGAAATAATGGAAAAAACAATAAAAGCAATAGCAAAAGAACA
AGAACCATATAAAGGAATATTATATGGTCAATTCATGTTATGTAAAGATGGTCCAAAAGTAATAGAATACAATGCAAGAT
TTGGAGATCCGGAATCTATGAATGTATTATCAGTAATAGATGATGATTTAGCAAAAATATCTAAACAAATAGTAGATGGA
ACATTAGATTCAGTGAATTTCCTTAATAAATCCTCAGTATGTAAGTATGTGGTACCTGATAAATATCCAAATACCCACGT
TGCAGATACTGTTGTTGATGTGGATGAAGATAAAATCAATGAATTAGGAGCACAAGTATTTTATGCAGCAGTTTATCAGG
ATATGGATGATTCTATTAAATTAACTTCATCAAGAGCATTAGGAGTACTTGCAGTAAAAGATTCTATTAAAGAAGCAGAA
AAAATCTGTGAAGAAGCTATAAAATATGTTAAAGGTGAAGTATACCATAGAAATGATGTTGCTACTGAAGAATTATTAAA
TGAAAAAATCAAACACATGGAAGAAGTTAGATTGTAA

Upstream 100 bases:

>100_bases
AATAAAATATTACGATAATAATTTTTTTTTTATTAAGTACAAATACAGTACAAAAACAACAAGTACAAAAAATACCTTAT
TAACAGGACGTGATTATATA

Downstream 100 bases:

>100_bases
TCTAACAACCTACTTTTTTTATTATCAAAATTAAATAAAAATCCATATAATTCTACACTACATTTTTAATAACTTAAATT
TTTACTACAAATACGAGTTA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKILMVGSGAREHAIAKALNKTADVYTYMGRKNPGLARISKNYTVNDESNFNEIIKFAQENNVELAFIGPEAPLEMGIVD
ELEKEGIHSVGPTKSAAQIETNKAFMRKLFEDYDIPGSIKYGTFYDLDEAYEFIDNFDEPVVVKPIGLTGGKGVKIVGDQ
LADNDEAKEYVKEIFHQKMGGFEGVVIEELLLGEEYTIQAFVDGTHLIPMPAAQDHPHAFVGNKGPITGGMGSYSDKNHL
LPFLTQEDYDKSVEIMEKTIKAIAKEQEPYKGILYGQFMLCKDGPKVIEYNARFGDPESMNVLSVIDDDLAKISKQIVDG
TLDSVNFLNKSSVCKYVVPDKYPNTHVADTVVDVDEDKINELGAQVFYAAVYQDMDDSIKLTSSRALGVLAVKDSIKEAE
KICEEAIKYVKGEVYHRNDVATEELLNEKIKHMEEVRL

Sequences:

>Translated_438_residues
MKILMVGSGAREHAIAKALNKTADVYTYMGRKNPGLARISKNYTVNDESNFNEIIKFAQENNVELAFIGPEAPLEMGIVD
ELEKEGIHSVGPTKSAAQIETNKAFMRKLFEDYDIPGSIKYGTFYDLDEAYEFIDNFDEPVVVKPIGLTGGKGVKIVGDQ
LADNDEAKEYVKEIFHQKMGGFEGVVIEELLLGEEYTIQAFVDGTHLIPMPAAQDHPHAFVGNKGPITGGMGSYSDKNHL
LPFLTQEDYDKSVEIMEKTIKAIAKEQEPYKGILYGQFMLCKDGPKVIEYNARFGDPESMNVLSVIDDDLAKISKQIVDG
TLDSVNFLNKSSVCKYVVPDKYPNTHVADTVVDVDEDKINELGAQVFYAAVYQDMDDSIKLTSSRALGVLAVKDSIKEAE
KICEEAIKYVKGEVYHRNDVATEELLNEKIKHMEEVRL
>Mature_438_residues
MKILMVGSGAREHAIAKALNKTADVYTYMGRKNPGLARISKNYTVNDESNFNEIIKFAQENNVELAFIGPEAPLEMGIVD
ELEKEGIHSVGPTKSAAQIETNKAFMRKLFEDYDIPGSIKYGTFYDLDEAYEFIDNFDEPVVVKPIGLTGGKGVKIVGDQ
LADNDEAKEYVKEIFHQKMGGFEGVVIEELLLGEEYTIQAFVDGTHLIPMPAAQDHPHAFVGNKGPITGGMGSYSDKNHL
LPFLTQEDYDKSVEIMEKTIKAIAKEQEPYKGILYGQFMLCKDGPKVIEYNARFGDPESMNVLSVIDDDLAKISKQIVDG
TLDSVNFLNKSSVCKYVVPDKYPNTHVADTVVDVDEDKINELGAQVFYAAVYQDMDDSIKLTSSRALGVLAVKDSIKEAE
KICEEAIKYVKGEVYHRNDVATEELLNEKIKHMEEVRL

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI28416899, Length=429, Percent_Identity=33.5664335664336, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI4503915, Length=429, Percent_Identity=33.5664335664336, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI209869995, Length=429, Percent_Identity=33.5664335664336, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI209869993, Length=429, Percent_Identity=33.5664335664336, Blast_Score=223, Evalue=2e-58,
Organism=Escherichia coli, GI1790438, Length=411, Percent_Identity=32.360097323601, Blast_Score=202, Evalue=4e-53,
Organism=Caenorhabditis elegans, GI17567511, Length=414, Percent_Identity=31.4009661835749, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6321203, Length=452, Percent_Identity=32.9646017699115, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI62471619, Length=437, Percent_Identity=31.5789473684211, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24582400, Length=439, Percent_Identity=31.4350797266515, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METST (Q2NI86)

Other databases:

- EMBL:   CP000102
- RefSeq:   YP_447092.1
- ProteinModelPortal:   Q2NI86
- SMR:   Q2NI86
- STRING:   Q2NI86
- GeneID:   3855038
- GenomeReviews:   CP000102_GR
- KEGG:   mst:Msp_0030
- NMPDR:   fig|339860.6.peg.29
- eggNOG:   arNOG04186
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- ProtClustDB:   PRK00885
- BioCyc:   MSTA339860:MSP_0030-MONOMER
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 48795; Mature: 48795

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILMVGSGAREHAIAKALNKTADVYTYMGRKNPGLARISKNYTVNDESNFNEIIKFAQE
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHC
NNVELAFIGPEAPLEMGIVDELEKEGIHSVGPTKSAAQIETNKAFMRKLFEDYDIPGSIK
CCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
YGTFYDLDEAYEFIDNFDEPVVVKPIGLTGGKGVKIVGDQLADNDEAKEYVKEIFHQKMG
CCCEECHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHC
GFEGVVIEELLLGEEYTIQAFVDGTHLIPMPAAQDHPHAFVGNKGPITGGMGSYSDKNHL
CCCCHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
LPFLTQEDYDKSVEIMEKTIKAIAKEQEPYKGILYGQFMLCKDGPKVIEYNARFGDPESM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCC
NVLSVIDDDLAKISKQIVDGTLDSVNFLNKSSVCKYVVPDKYPNTHVADTVVDVDEDKIN
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCHHHHEEECCHHHHH
ELGAQVFYAAVYQDMDDSIKLTSSRALGVLAVKDSIKEAEKICEEAIKYVKGEVYHRNDV
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCH
ATEELLNEKIKHMEEVRL
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKILMVGSGAREHAIAKALNKTADVYTYMGRKNPGLARISKNYTVNDESNFNEIIKFAQE
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCEEECCCCCHHHHHHHHHC
NNVELAFIGPEAPLEMGIVDELEKEGIHSVGPTKSAAQIETNKAFMRKLFEDYDIPGSIK
CCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
YGTFYDLDEAYEFIDNFDEPVVVKPIGLTGGKGVKIVGDQLADNDEAKEYVKEIFHQKMG
CCCEECHHHHHHHHHCCCCCEEEEEECCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHC
GFEGVVIEELLLGEEYTIQAFVDGTHLIPMPAAQDHPHAFVGNKGPITGGMGSYSDKNHL
CCCCHHHHHHHCCCCEEEEEEECCCEEECCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
LPFLTQEDYDKSVEIMEKTIKAIAKEQEPYKGILYGQFMLCKDGPKVIEYNARFGDPESM
CCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEECCCCCEEEECCCCCCCCCC
NVLSVIDDDLAKISKQIVDGTLDSVNFLNKSSVCKYVVPDKYPNTHVADTVVDVDEDKIN
CHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCEEEECCCCCCCCCHHHHEEECCHHHHH
ELGAQVFYAAVYQDMDDSIKLTSSRALGVLAVKDSIKEAEKICEEAIKYVKGEVYHRNDV
HHHHHHHHHHHHHCCCCCEEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHCCCEEECCCH
ATEELLNEKIKHMEEVRL
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA