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The map label for this gene is ogt [H]

Identifier: 83721435

GI number: 83721435

Start: 834715

End: 835182

Strand: Direct

Name: ogt [H]

Synonym: BTH_I0725

Alternate gene names: 83721435

Gene position: 834715-835182 (Clockwise)

Preceding gene: 83719067

Following gene: 83720485

Centisome position: 21.91

GC content: 64.96

Gene sequence:

>468_bases
ATGTTCAACGCAGTAATCGATGCGCCATTCGGCAAGATCGGCATCCGCACCGACGCGTCGGTCGTGCGCGAGATCGTCTA
CTTGCCCGAATCGATGAAGAGCGTCGCGCCGGACACGACGCTCGCGATGCGGGTGGCGCGTCAGATCGAGCGTTATTTCG
AGCGCGCATCCGCGCATTTCGACTTGCCGCTCGCCGAAGTCGGCACGCCGTTCCAGCATCGCGTGTGGAACGCGATCTGC
GCGATTCCGCCCGGCGTCGTGCTGACCTACGGGCAGATCGCGAAACAGATCGGCAGCGCGCCGCGCGCGGTCGGCCAGGC
CTGCGGGGCGAACTATTTTCCGCTCGTGATTCCGTGCCATCGCGTCGTCGCGTCCGGCGGGATCGGCGGCTTCGCGAACC
ACGACGACGAAGGCTATTTCCTGAAGGTCAAGCGCTGGTTGCTCGCGCATGAGGGCGTGCGGTTCTGA

Upstream 100 bases:

>100_bases
CACGTCGAGTGGGCGCTGCGCGCCGCCTGAAGGCGGCGTAAAGTGGCGGTTTGGCGGGCGGCCCGCGCATTGCGCGGGCG
CGTCCTTTCAGGAGGGAACG

Downstream 100 bases:

>100_bases
TGGCGCGCGCAGCACGCATTCCCGCGCCCGGCCCGGCGAGCGGCGGCGCCGGCCGGGGGGCCGCGCCGTCGGCCGATCGC
CCGGCCGACGAGTTGGCCGC

Product: methylated-DNA-protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 155; Mature: 155

Protein sequence:

>155_residues
MFNAVIDAPFGKIGIRTDASVVREIVYLPESMKSVAPDTTLAMRVARQIERYFERASAHFDLPLAEVGTPFQHRVWNAIC
AIPPGVVLTYGQIAKQIGSAPRAVGQACGANYFPLVIPCHRVVASGGIGGFANHDDEGYFLKVKRWLLAHEGVRF

Sequences:

>Translated_155_residues
MFNAVIDAPFGKIGIRTDASVVREIVYLPESMKSVAPDTTLAMRVARQIERYFERASAHFDLPLAEVGTPFQHRVWNAIC
AIPPGVVLTYGQIAKQIGSAPRAVGQACGANYFPLVIPCHRVVASGGIGGFANHDDEGYFLKVKRWLLAHEGVRF
>Mature_155_residues
MFNAVIDAPFGKIGIRTDASVVREIVYLPESMKSVAPDTTLAMRVARQIERYFERASAHFDLPLAEVGTPFQHRVWNAIC
AIPPGVVLTYGQIAKQIGSAPRAVGQACGANYFPLVIPCHRVVASGGIGGFANHDDEGYFLKVKRWLLAHEGVRF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=176, Percent_Identity=31.25, Blast_Score=69, Evalue=2e-12,
Organism=Escherichia coli, GI1787596, Length=112, Percent_Identity=41.9642857142857, Blast_Score=81, Evalue=3e-17,
Organism=Escherichia coli, GI1788542, Length=117, Percent_Identity=35.042735042735, Blast_Score=70, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320001, Length=88, Percent_Identity=45.4545454545455, Blast_Score=75, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 16968; Mature: 16968

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNAVIDAPFGKIGIRTDASVVREIVYLPESMKSVAPDTTLAMRVARQIERYFERASAHF
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
DLPLAEVGTPFQHRVWNAICAIPPGVVLTYGQIAKQIGSAPRAVGQACGANYFPLVIPCH
CCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVASGGIGGFANHDDEGYFLKVKRWLLAHEGVRF
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFNAVIDAPFGKIGIRTDASVVREIVYLPESMKSVAPDTTLAMRVARQIERYFERASAHF
CCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
DLPLAEVGTPFQHRVWNAICAIPPGVVLTYGQIAKQIGSAPRAVGQACGANYFPLVIPCH
CCCHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHH
RVVASGGIGGFANHDDEGYFLKVKRWLLAHEGVRF
HHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]