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The map label for this gene is lgrE [H]

Identifier: 83721366

GI number: 83721366

Start: 2655707

End: 2656543

Strand: Reverse

Name: lgrE [H]

Synonym: BTH_I2357

Alternate gene names: 83721366

Gene position: 2656543-2655707 (Counterclockwise)

Preceding gene: 83720118

Following gene: 83720639

Centisome position: 69.74

GC content: 65.23

Gene sequence:

>837_bases
GTGACCTTCGAATCGACGGAGAAAGCCGGCGCCGGCCGCTGGATCGAGCCGTCACCGTCGGCCGTGAACGCCGTGCAGCG
GTTGTTCTGCATCCCTTATGCGGGCGGCGGCGCATCCGTGTTCCGCGGCTGGCCGGCACGGCTTGGGGCGACGGTCGACG
TGCGTCCGCTGCATCCGCCCGGGCGAGGCCGCCGTTTCCGCGATGCGTTGCTATATCGGGTGGACCGGATCGCCGACGAG
GTGACGGAGGCGATCGCGCCGCTGCTCGACCGGCCCTATACCCTGTTCGGCCACAGCATGGGCGCCAGCGTGGCGTTCGA
GGTGGCGTCACGCATCCAGGCCCGCCGCCTGCGGGAGCCGACGTGTTTGATCGTTTCCGGCCGCGGCGCTCCGCATCTGC
CTACGCAGCGGCGGCCGATACACGATTTGCCGGACGATCAATTTCTGGACGAGGTGATACGCATGAACGGCACGCCGCCT
GAAATTCTCGCGCATCCCGAAGTGATCACATTGCTACTCCCGATTCTTCGTGCCGATTTCAAGGCGGTCGAGACTTACCG
GGCGGAACCGCATCCCCCTCTGAACTGCCGGATCGTTGCACTGGGGGGCAGTGGCGAGCCGGACAGCGCGGAGGCGGTGG
AGTCGTGGCGCACTTACACGACCGGCGGCTTTCGCGTCGAGATCCTTCCCGGCGATCACTTTTTTATACATGGCCATGCT
GAGCGGCTGTTTTCAGTTCTCGGCGAGGAATTGACGGATGCGCTGCGTTGCGCCGCAACCCCGTTGGCAACACCGGTGCA
TCAGCCATCCGCAGATTCATGTGATGCGCCGAAGTGA

Upstream 100 bases:

>100_bases
GGCATCGGCTTCCGCACGCCTGGCCCACGCTGACGCCTTATCTGCCCGAAGCGAAGGCGGCCGTAAAAGAGGCCACCGAC
TTTATTCGGAGGGTGGTGCC

Downstream 100 bases:

>100_bases
TCGCGCCAAGACTCGGAACGCAACACCGCCAGATATCCGCATCATGTCAAAGCCATTCCTGAAGGTCCGCTTTCCTGTGC
GGCAGCGAAGTCCGGTCTTT

Product: thioesterase type II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTFESTEKAGAGRWIEPSPSAVNAVQRLFCIPYAGGGASVFRGWPARLGATVDVRPLHPPGRGRRFRDALLYRVDRIADE
VTEAIAPLLDRPYTLFGHSMGASVAFEVASRIQARRLREPTCLIVSGRGAPHLPTQRRPIHDLPDDQFLDEVIRMNGTPP
EILAHPEVITLLLPILRADFKAVETYRAEPHPPLNCRIVALGGSGEPDSAEAVESWRTYTTGGFRVEILPGDHFFIHGHA
ERLFSVLGEELTDALRCAATPLATPVHQPSADSCDAPK

Sequences:

>Translated_278_residues
MTFESTEKAGAGRWIEPSPSAVNAVQRLFCIPYAGGGASVFRGWPARLGATVDVRPLHPPGRGRRFRDALLYRVDRIADE
VTEAIAPLLDRPYTLFGHSMGASVAFEVASRIQARRLREPTCLIVSGRGAPHLPTQRRPIHDLPDDQFLDEVIRMNGTPP
EILAHPEVITLLLPILRADFKAVETYRAEPHPPLNCRIVALGGSGEPDSAEAVESWRTYTTGGFRVEILPGDHFFIHGHA
ERLFSVLGEELTDALRCAATPLATPVHQPSADSCDAPK
>Mature_277_residues
TFESTEKAGAGRWIEPSPSAVNAVQRLFCIPYAGGGASVFRGWPARLGATVDVRPLHPPGRGRRFRDALLYRVDRIADEV
TEAIAPLLDRPYTLFGHSMGASVAFEVASRIQARRLREPTCLIVSGRGAPHLPTQRRPIHDLPDDQFLDEVIRMNGTPPE
ILAHPEVITLLLPILRADFKAVETYRAEPHPPLNCRIVALGGSGEPDSAEAVESWRTYTTGGFRVEILPGDHFFIHGHAE
RLFSVLGEELTDALRCAATPLATPVHQPSADSCDAPK

Specific function: In the final step of gramicidin biosynthesis, reduces the pentadecapeptide-aldehyde intermediate, that is released from the terminal module of the non-ribosomal peptide synthetase LgrD, to the final product ethanolamine-containing gramicidin [H]

COG id: COG3208

COG function: function code Q; Predicted thioesterase involved in non-ribosomal peptide biosynthesis

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase family [H]

Homologues:

Organism=Homo sapiens, GI89257335, Length=222, Percent_Identity=30.6306306306306, Blast_Score=112, Evalue=3e-25,
Organism=Homo sapiens, GI8922871, Length=192, Percent_Identity=30.2083333333333, Blast_Score=92, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012223
- InterPro:   IPR001031 [H]

Pfam domain/function: PF00975 Thioesterase [H]

EC number: NA

Molecular weight: Translated: 30432; Mature: 30301

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFESTEKAGAGRWIEPSPSAVNAVQRLFCIPYAGGGASVFRGWPARLGATVDVRPLHPP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCEEEEEECCCC
GRGRRFRDALLYRVDRIADEVTEAIAPLLDRPYTLFGHSMGASVAFEVASRIQARRLREP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCC
TCLIVSGRGAPHLPTQRRPIHDLPDDQFLDEVIRMNGTPPEILAHPEVITLLLPILRADF
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
KAVETYRAEPHPPLNCRIVALGGSGEPDSAEAVESWRTYTTGGFRVEILPGDHFFIHGHA
HHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCH
ERLFSVLGEELTDALRCAATPLATPVHQPSADSCDAPK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TFESTEKAGAGRWIEPSPSAVNAVQRLFCIPYAGGGASVFRGWPARLGATVDVRPLHPP
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCHHHHCCCHHHHCCEEEEEECCCC
GRGRRFRDALLYRVDRIADEVTEAIAPLLDRPYTLFGHSMGASVAFEVASRIQARRLREP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCC
TCLIVSGRGAPHLPTQRRPIHDLPDDQFLDEVIRMNGTPPEILAHPEVITLLLPILRADF
CEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHHHHH
KAVETYRAEPHPPLNCRIVALGGSGEPDSAEAVESWRTYTTGGFRVEILPGDHFFIHGHA
HHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCH
ERLFSVLGEELTDALRCAATPLATPVHQPSADSCDAPK
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA