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The map label for this gene is bah [H]

Identifier: 83720118

GI number: 83720118

Start: 2656540

End: 2657460

Strand: Reverse

Name: bah [H]

Synonym: BTH_I2358

Alternate gene names: 83720118

Gene position: 2657460-2656540 (Counterclockwise)

Preceding gene: 83720445

Following gene: 83721366

Centisome position: 69.76

GC content: 62.11

Gene sequence:

>921_bases
ATGATCGTGAGTTGGCAAAACTGGTGCTTTTCCTGGCTATCGCGTAGAACGACCAAGTCCCTGACCAGAAAACCGGCGCT
GTCCGCTCACGACATGCGAGCGCACAGCGCCAAATGGTCCAGCCGTGGCGTGAAACCTCCCCGGGGCTGGCGCGTGCGCG
AAATGACCGCGCGGCCGCTATGTGGCGAATGGATCGAACCGGCGGACCCGGTTCGCCGCGACTCGCTTCGCCGCATCGTC
ATTTATTTGCATGGCGGAGGATATTGCTTTTGCTCTCCCCGCACGCATCGAACGATTGCCTGTGCGTTGGCCAAAGGGGC
GGACGCGCGCACCTTTTCATTGGATTACCGGCTTGCTCCGGAACATCCGTTTCCCGCCGCGGTGGAGGATGCATTGGCCT
CCTATCGGCAACTTCTCGCCGATGGCGCATCTTCAGACCGGATCGTCATCGGTGGGGATTCCTCCGGCGGGGGGCTCGCG
CTTGCTCTGTTGCTGTCGTTGCGCGATGCCGGTGATCCGATGCCGGCTGGCGCGGTATTGTTTTCTCCGTGGACGGATCT
GGCCGCGACCGGCGCGTCCCTGAAGGCGAATGACGAAGCCGATGTCATGCTGACCGCAGCCGCCGTAGCGAATTTTTCCC
AGTATTATCTGGGCGGCACAGCTGCTGATCACCCTATTGCTTCACCTCTCTATGGAGAATATGCGGGTTTGCCGCCCTTG
TTCGTCCAGGCGAGCGACACGGAGGTATTGCTGGACGACGCCGCGCGGGTCGCGGAAAAGGCCCGCGTTGCCGACGTCGC
GGTGGACTTCAAGGTCTGGCATCGGCTTCCGCACGCCTGGCCCACGCTGACGCCTTATCTGCCCGAAGCGAAGGCGGCCG
TAAAAGAGGCCACCGACTTTATTCGGAGGGTGGTGCCGTGA

Upstream 100 bases:

>100_bases
CATCGTCGCGCATCGAAAGCTGGTCATTCCTTGATGCGTCGGAGAGTCCATTCGGACGACAACTTTTCGAGCGACCGATT
TTTCTTCATCTACCGGGAAC

Downstream 100 bases:

>100_bases
CCTTCGAATCGACGGAGAAAGCCGGCGCCGGCCGCTGGATCGAGCCGTCACCGTCGGCCGTGAACGCCGTGCAGCGGTTG
TTCTGCATCCCTTATGCGGG

Product: lipase/esterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MIVSWQNWCFSWLSRRTTKSLTRKPALSAHDMRAHSAKWSSRGVKPPRGWRVREMTARPLCGEWIEPADPVRRDSLRRIV
IYLHGGGYCFCSPRTHRTIACALAKGADARTFSLDYRLAPEHPFPAAVEDALASYRQLLADGASSDRIVIGGDSSGGGLA
LALLLSLRDAGDPMPAGAVLFSPWTDLAATGASLKANDEADVMLTAAAVANFSQYYLGGTAADHPIASPLYGEYAGLPPL
FVQASDTEVLLDDAARVAEKARVADVAVDFKVWHRLPHAWPTLTPYLPEAKAAVKEATDFIRRVVP

Sequences:

>Translated_306_residues
MIVSWQNWCFSWLSRRTTKSLTRKPALSAHDMRAHSAKWSSRGVKPPRGWRVREMTARPLCGEWIEPADPVRRDSLRRIV
IYLHGGGYCFCSPRTHRTIACALAKGADARTFSLDYRLAPEHPFPAAVEDALASYRQLLADGASSDRIVIGGDSSGGGLA
LALLLSLRDAGDPMPAGAVLFSPWTDLAATGASLKANDEADVMLTAAAVANFSQYYLGGTAADHPIASPLYGEYAGLPPL
FVQASDTEVLLDDAARVAEKARVADVAVDFKVWHRLPHAWPTLTPYLPEAKAAVKEATDFIRRVVP
>Mature_306_residues
MIVSWQNWCFSWLSRRTTKSLTRKPALSAHDMRAHSAKWSSRGVKPPRGWRVREMTARPLCGEWIEPADPVRRDSLRRIV
IYLHGGGYCFCSPRTHRTIACALAKGADARTFSLDYRLAPEHPFPAAVEDALASYRQLLADGASSDRIVIGGDSSGGGLA
LALLLSLRDAGDPMPAGAVLFSPWTDLAATGASLKANDEADVMLTAAAVANFSQYYLGGTAADHPIASPLYGEYAGLPPL
FVQASDTEVLLDDAARVAEKARVADVAVDFKVWHRLPHAWPTLTPYLPEAKAAVKEATDFIRRVVP

Specific function: This protein removes the N-acetyl group from bialaphos as one of the final steps of the bialaphos biosynthetic pathway [H]

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=222, Percent_Identity=29.7297297297297, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI21328446, Length=152, Percent_Identity=28.9473684210526, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1786682, Length=234, Percent_Identity=28.2051282051282, Blast_Score=71, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17567059, Length=289, Percent_Identity=24.9134948096886, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533410, Length=95, Percent_Identity=35.7894736842105, Blast_Score=67, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI115533412, Length=114, Percent_Identity=33.3333333333333, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24656084, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI24656076, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=7e-11,
Organism=Drosophila melanogaster, GI20130169, Length=106, Percent_Identity=33.9622641509434, Blast_Score=65, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 33265; Mature: 33265

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS01173 LIPASE_GDXG_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIVSWQNWCFSWLSRRTTKSLTRKPALSAHDMRAHSAKWSSRGVKPPRGWRVREMTARPL
CEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCCCCCEEHHHCCCCC
CGEWIEPADPVRRDSLRRIVIYLHGGGYCFCSPRTHRTIACALAKGADARTFSLDYRLAP
CCCCCCCCCHHHHHCCEEEEEEEECCCEEEECCCCCCEEEEEECCCCCCEEEEEEEEECC
EHPFPAAVEDALASYRQLLADGASSDRIVIGGDSSGGGLALALLLSLRDAGDPMPAGAVL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
FSPWTDLAATGASLKANDEADVMLTAAAVANFSQYYLGGTAADHPIASPLYGEYAGLPPL
ECCCHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCE
FVQASDTEVLLDDAARVAEKARVADVAVDFKVWHRLPHAWPTLTPYLPEAKAAVKEATDF
EEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IRRVVP
HHHHCC
>Mature Secondary Structure
MIVSWQNWCFSWLSRRTTKSLTRKPALSAHDMRAHSAKWSSRGVKPPRGWRVREMTARPL
CEECCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHCCCCCCCCCEEHHHCCCCC
CGEWIEPADPVRRDSLRRIVIYLHGGGYCFCSPRTHRTIACALAKGADARTFSLDYRLAP
CCCCCCCCCHHHHHCCEEEEEEEECCCEEEECCCCCCEEEEEECCCCCCEEEEEEEEECC
EHPFPAAVEDALASYRQLLADGASSDRIVIGGDSSGGGLALALLLSLRDAGDPMPAGAVL
CCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCCCCEEE
FSPWTDLAATGASLKANDEADVMLTAAAVANFSQYYLGGTAADHPIASPLYGEYAGLPPL
ECCCHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCE
FVQASDTEVLLDDAARVAEKARVADVAVDFKVWHRLPHAWPTLTPYLPEAKAAVKEATDF
EEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHH
IRRVVP
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2066341 [H]