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The map label for this gene is insH10 [H]

Identifier: 83720639

GI number: 83720639

Start: 2646265

End: 2646903

Strand: Reverse

Name: insH10 [H]

Synonym: BTH_I2350

Alternate gene names: 83720639

Gene position: 2646903-2646265 (Counterclockwise)

Preceding gene: 83721366

Following gene: 83720568

Centisome position: 69.49

GC content: 58.22

Gene sequence:

>639_bases
ATGAAACAACTGACACCGGCGATGGCGGTCGATCAATGCGCGGGATTCAGAGCACAGCGCAAACCTACGCGACGTGAGGC
TTTTCTCGATGAGATGAACAGGCTCGTGCCGTGGGCCGAACTGTGCGTGGTGGTCGAGCCGTATTACCCGAAACGCGGCA
ACGGTCGGCCGCCAATCGCGCTGGAGAGCATGTTGCGAATTCATTTTGTGCAGCACTGGTTCAACCTTGCCGACCTTGCG
TGTGAAGAGGCGCTGTACGACAGCCTCAGCCTGCGCCGTTTTGTGGGCATTGACCTGGGCAGCGAAACCGTTCCGGACGC
GACGACGCTGCTCAAATTCCGGCACCTGCTGGAAGAGCACAAACTGGGCGAGCGAATCTTCGCAGAGGTCGGCCGGGTGT
TGCAGGCACGCGGAGCAAAGCTCAAGAGCGGCACGATTGTGGATGCCACGCTGATCGGCGCGCCTTCTTCGACGAAGAAC
AGGGAGAAAGCGCGGGACCCTGAAATGCATCAGACGCGTAAAGGCCAGCAGTGGTATTTCGGCATGAAGATTCATGTCGG
CGTGGATATACGATTCGACGCACCTCGGGCGGATAGGCAAAGAAGGGAATCACCTGTTCCGAGCCTGTCAGATTTTTAG

Upstream 100 bases:

>100_bases
GTCAACGTGAGCTATTTCCGTTAAGGCAATGCTGATCAAGTCCACCACTTATGGCTGTCATGCGTTATAGCGTGGACATG
CGACGGTAAAAGCAAAAACG

Downstream 100 bases:

>100_bases
TGTGCTGAGGTCATGAGACGATAACGGAACTAACGTCCTATGACCGATGCAACAGTGAGCAAGAAGAGCAAGAATCCGAA
GGCACCAAAGCTGTTTCCCG

Product: ISPsy10, transposase, truncation

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MKQLTPAMAVDQCAGFRAQRKPTRREAFLDEMNRLVPWAELCVVVEPYYPKRGNGRPPIALESMLRIHFVQHWFNLADLA
CEEALYDSLSLRRFVGIDLGSETVPDATTLLKFRHLLEEHKLGERIFAEVGRVLQARGAKLKSGTIVDATLIGAPSSTKN
REKARDPEMHQTRKGQQWYFGMKIHVGVDIRFDAPRADRQRRESPVPSLSDF

Sequences:

>Translated_212_residues
MKQLTPAMAVDQCAGFRAQRKPTRREAFLDEMNRLVPWAELCVVVEPYYPKRGNGRPPIALESMLRIHFVQHWFNLADLA
CEEALYDSLSLRRFVGIDLGSETVPDATTLLKFRHLLEEHKLGERIFAEVGRVLQARGAKLKSGTIVDATLIGAPSSTKN
REKARDPEMHQTRKGQQWYFGMKIHVGVDIRFDAPRADRQRRESPVPSLSDF
>Mature_212_residues
MKQLTPAMAVDQCAGFRAQRKPTRREAFLDEMNRLVPWAELCVVVEPYYPKRGNGRPPIALESMLRIHFVQHWFNLADLA
CEEALYDSLSLRRFVGIDLGSETVPDATTLLKFRHLLEEHKLGERIFAEVGRVLQARGAKLKSGTIVDATLIGAPSSTKN
REKARDPEMHQTRKGQQWYFGMKIHVGVDIRFDAPRADRQRRESPVPSLSDF

Specific function: Involved in the transposition of the insertion sequence IS5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 11 family [H]

Homologues:

Organism=Escherichia coli, GI1787634, Length=176, Percent_Identity=53.9772727272727, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI1789921, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1789612, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1789355, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1788518, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1788342, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1787592, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1786877, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1786764, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1786453, Length=176, Percent_Identity=53.9772727272727, Blast_Score=215, Evalue=2e-57,
Organism=Escherichia coli, GI1788303, Length=176, Percent_Identity=53.9772727272727, Blast_Score=214, Evalue=2e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002559
- InterPro:   IPR008490 [H]

Pfam domain/function: PF05598 DUF772; PF01609 Transposase_11 [H]

EC number: NA

Molecular weight: Translated: 24199; Mature: 24199

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQLTPAMAVDQCAGFRAQRKPTRREAFLDEMNRLVPWAELCVVVEPYYPKRGNGRPPIA
CCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCHH
LESMLRIHFVQHWFNLADLACEEALYDSLSLRRFVGIDLGSETVPDATTLLKFRHLLEEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
KLGERIFAEVGRVLQARGAKLKSGTIVDATLIGAPSSTKNREKARDPEMHQTRKGQQWYF
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHCCCCCEEE
GMKIHVGVDIRFDAPRADRQRRESPVPSLSDF
EEEEEEEEEEEECCCCHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKQLTPAMAVDQCAGFRAQRKPTRREAFLDEMNRLVPWAELCVVVEPYYPKRGNGRPPIA
CCCCCHHHHHHHHHCCCHHCCCHHHHHHHHHHHHHCCHHHHHEEECCCCCCCCCCCCCHH
LESMLRIHFVQHWFNLADLACEEALYDSLSLRRFVGIDLGSETVPDATTLLKFRHLLEEH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHH
KLGERIFAEVGRVLQARGAKLKSGTIVDATLIGAPSSTKNREKARDPEMHQTRKGQQWYF
HHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCCHHHHCCCHHHHHCCCCCEEE
GMKIHVGVDIRFDAPRADRQRRESPVPSLSDF
EEEEEEEEEEEECCCCHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9097039; 9097040; 9278503 [H]