Click here to switch to the map view.

The map label for this gene is dnaN [H]

Identifier: 83720585

GI number: 83720585

Start: 3692120

End: 3693223

Strand: Direct

Name: dnaN [H]

Synonym: BTH_I3240

Alternate gene names: 83720585

Gene position: 3692120-3693223 (Clockwise)

Preceding gene: 83719949

Following gene: 161723181

Centisome position: 96.93

GC content: 63.41

Gene sequence:

>1104_bases
ATGCAACTGGTCAAGACCGAACGAGACACCCTCCTTAGGCCGCTGCAAACCGTGAGCGGTATCGTCGAACGCCGCCACAC
GTTGCCGATCCTCGCCAATCTGTTGATCACGAAGAACGGCCCGGACGTGTCGTTCCTGTCGACCGACCTCGAGCTCCAGA
TCACGACGCGCGCCGATTTCGGCGTCGGCGGCGACCAGGTCGCGACGACCGTCGCGGCCCGCAAGCTCCTCGACATCCTG
CGTGCGATGCCCGACGGCCAGGTCACGCTGTCGCTCGCCGACAAGCGCCTGACGGTCCAGTCCGGCAAGAGCCGCTTCGC
CCTGCAAACGCTCGCCGCCGACGAGTTCCCGACCGTCGCGCAATCGAAAGACTTCGGCGCGAGCCTCGCCGTTCCGCAAA
AGGCGTTCCGCCAGTTGCTCGGCATGGTGTACTTCGCGATGGCGCAGCAGGATATCCGCTACTACCTGAACGGGATGCTG
CTCGTCGTGGACGGCGACCGCCTGATGGCTGTCGCGACCGACGGCCACCGCCTCGCGTTCTCGTCGATGAAGATCGAAGG
CTCGTTCAGCCGCCAGGAAGTGATCGTGCCGCGCAAGACGATTCTCGAGCTGCAGCGCCTGCTCGAGGACATCGACGACA
CGGTCAAGATCGACATCGCGCAGACTCAGGCGAAGTTCACGTTCGGCCAGGTCGAGCTCGTATCGAAGCTCGTCGAGGGC
AAGTTCCCGGACTTCCAGCGCGTGATTCCGAAGGCGCACAAGAACACGTTCGAAATCGGCCGCGAAGAGCTGCAGCGCTC
GCTGCAGCGAGCGGCGATCCTCACGTCCGACAAGTTCAAGGGCGTGCGCTGCATCATCGCGCCCGGCCAGTTGAAGATCA
TGTCGACGAACGCCGACCAGGAAGAGGCGCAGGAAGAACTGGAAATCGCCTACCAGGGCGACACCGTCGACATCGGCTTC
AACGTCACGTATCTGCTCGACGTGCTCGCGAACCTGAAGGTCGACACCGTTCAGGTGAGCCTCGGCGACGCGAGCTCGAG
CGCCCTCATCACCGTGCCCGAGAACGACGAATTCAAGTACGTGGTGATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
GGCACAATACTGGTTTGACCGCCCGGCCGGCGGCGGGCCGAGGGCCCGCCCGGCCAGGCGCTGCGAGGCTTGCAGGCCGT
TAATTGAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGCACGACTCGCCGGACACACCAAGGGGCGCCACGCCCCTTTGGCGTTTTTATGGCGATTCGCACGCTTGAGCGCCGG
CGCGCGACGCCGGCCCCCGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFSRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Sequences:

>Translated_367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFSRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI
>Mature_367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFSRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=364, Percent_Identity=43.956043956044, Blast_Score=303, Evalue=1e-83,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40710; Mature: 40710

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCCC
QSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGMLLVVDGDRLMAVATDGHRLAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIEGSFSRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
EEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEHHHHHHHHHHHCC
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCH
EEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKY
HHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC
>Mature Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCCC
QSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGMLLVVDGDRLMAVATDGHRLAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIEGSFSRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
EEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEHHHHHHHHHHHCC
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCH
EEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKY
HHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]