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The map label for this gene is glpK [H]

Identifier: 83719921

GI number: 83719921

Start: 682381

End: 683883

Strand: Reverse

Name: glpK [H]

Synonym: BTH_I0599

Alternate gene names: 83719921

Gene position: 683883-682381 (Counterclockwise)

Preceding gene: 83720139

Following gene: 83719155

Centisome position: 17.95

GC content: 66.53

Gene sequence:

>1503_bases
ATGCAGGATCAATACATCCTCGCGCTCGACCAGGGCACGACCAGCTCCCGCGCCATGCTGTTCGATCGACAAGGCAACAT
CGTGTCGATCGCGCAGAAGGAATTCGAGCAAATTTATCCGCGGCCGGGCTGGGTCGAGCACGATCCGCAGGAGATCTGGT
CGACGCAGGCGGGCGTCGCGGCCGAGGCCGTCACGCGCGTCGGCCTGAACGGCACGTCGATCGCCGCGATCGGCATCACG
AACCAGCGCGAGACGACGATCGTCTGGGATCGCGAGACGGGCCATCCAATCTACAACGCAATCGTCTGGCAGGATCGCCG
CACCGCCGATTTCTGCGACCAGTTGAAAGCGCAGGGCCTGAGCGAGAAGGTGCGCGCGAAGACGGGGCTGCCGATCGATT
CGTACTTCTCGGCCACCAAGATCCGCTGGATTCTCGACAACGTCGAAGGCGCGCGCGAGAAGGCGCGCCAGGGCAAGCTC
GCGTTCGGCACGGTCGACAGCTGGCTCGTCTGGAACTTCACGAAGCACGAGCTGCACGTGACCGACGTGACGAACGCGTC
GCGCACGATGCTCTTCAACATCCACACGCTCGACTGGGACGACGAACTGCTCGACGCGCTCGAGATCCCGCGCAGCATGC
TGCCGCAAGTGCGCGCGTCGTCGGAGATCTACGGGCCGACGAAGACCACCGTGTTCGCCTCGAAGATTCCGCTCGCCGGC
ATCGCGGGCGACCAGCAGGCCGCCCTCTTCGGCCAGATGTGCACGAGCTCCGGCATGGTGAAGAACACCTACGGCACCGG
CTGCTTCCTGATGATGAACACCGGCGAGACGCCGATCGAATCACAGAACAACCTCGTCACGACGATCGCATGGCAGGTCG
ACGGCAAGGTCAACTACGCGCTCGAAGGCAGCATCTTCATCGCGGGCGCGGTCGTGCAATGGCTGCGCGACGGGCTCGGG
ATCATCAAGAGCGCGTCGGAAATCGAAGCGCTCGCGGCGGGCGTGCCTCACACGGACGGCGTCTATCTCGTGCCCGCGTT
CGCCGGCCTCGGCGCGCCGCACTGGAACGCGCGCGCGCGCGGATCGCTCTTCGGCGTCACGCGCGGCACGACGTCCGCGC
ACCTCGCGCGCGCGGCGCTCGATTCGATCGCGTATCAGTCGCTCGACGTGCTGAAGGCGATGGAAGCCGACTCGGGCATC
CGGATCGGCGAGCTGCGCGTGGACGGCGGCGCAAGCGCGAACAATCTGCTGATGCAGTTCCAGGCGGATCTGCTCGGCGT
CGACGCGGTGCGCCCGCGCGTCACCGAGACGACTGCGCTCGGCGCCGCGTATCTCGCGGGCCTTGCGATCGGCTACTGGA
AGAACGTCGACGAGCTGCACAGCCAATGGCAGCTCGAGCGCCGCTTCGCGCCGTCGATGCAGCGCGAGCAGGTGAGCTCG
TGCCTCTGCGGCTGGCAGCGCGCGGTGCGCGCGGCGAAGGCATGGGCAGACGACACGCAGTAA

Upstream 100 bases:

>100_bases
GCGCACGACGCCGGCAACGACGAACGACGACAGGGAAACATCAGGAATGCGCCGCGCCCGCGGCGCCAATCACAACTACG
TACGGATGGAGATGAGACAG

Downstream 100 bases:

>100_bases
GCCCGTTCGTTCGGCATCCGTATCGATCTCATTTCAACGACACCATTCACAACAATCAGGCGGGCCGCGCGACGCAAGCC
GCGAGCCCGCCATACCGGTA

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPRPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTSIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESQNNLVTTIAWQVDGKVNYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRVTETTALGAAYLAGLAIGYWKNVDELHSQWQLERRFAPSMQREQVSS
CLCGWQRAVRAAKAWADDTQ

Sequences:

>Translated_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPRPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTSIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESQNNLVTTIAWQVDGKVNYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRVTETTALGAAYLAGLAIGYWKNVDELHSQWQLERRFAPSMQREQVSS
CLCGWQRAVRAAKAWADDTQ
>Mature_500_residues
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPRPGWVEHDPQEIWSTQAGVAAEAVTRVGLNGTSIAAIGIT
NQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGLSEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKL
AFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESQNNLVTTIAWQVDGKVNYALEGSIFIAGAVVQWLRDGLG
IIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARARGSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGI
RIGELRVDGGASANNLLMQFQADLLGVDAVRPRVTETTALGAAYLAGLAIGYWKNVDELHSQWQLERRFAPSMQREQVSS
CLCGWQRAVRAAKAWADDTQ

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=45.6175298804781, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI4504007, Length=501, Percent_Identity=45.7085828343313, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.1676528599605, Blast_Score=439, Evalue=1e-123,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.8207171314741, Blast_Score=437, Evalue=1e-123,
Organism=Homo sapiens, GI88196792, Length=511, Percent_Identity=31.5068493150685, Blast_Score=306, Evalue=3e-83,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=60.5633802816901, Blast_Score=659, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=504, Percent_Identity=25.3968253968254, Blast_Score=115, Evalue=9e-27,
Organism=Escherichia coli, GI1789168, Length=490, Percent_Identity=25.5102040816327, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789987, Length=482, Percent_Identity=24.6887966804979, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787789, Length=499, Percent_Identity=24.8496993987976, Blast_Score=93, Evalue=5e-20,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=41.8, Blast_Score=400, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI71986140, Length=515, Percent_Identity=24.2718446601942, Blast_Score=175, Evalue=4e-44,
Organism=Caenorhabditis elegans, GI71986147, Length=255, Percent_Identity=30.5882352941176, Blast_Score=126, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6321755, Length=491, Percent_Identity=37.8818737270876, Blast_Score=309, Evalue=7e-85,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=41.980198019802, Blast_Score=398, Evalue=1e-111,
Organism=Drosophila melanogaster, GI24655555, Length=478, Percent_Identity=41.4225941422594, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655560, Length=478, Percent_Identity=41.4225941422594, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655570, Length=478, Percent_Identity=41.4225941422594, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655574, Length=478, Percent_Identity=41.4225941422594, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24655565, Length=478, Percent_Identity=41.4225941422594, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=42.4719101123596, Blast_Score=366, Evalue=1e-101,
Organism=Drosophila melanogaster, GI19922058, Length=532, Percent_Identity=32.7067669172932, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24652871, Length=499, Percent_Identity=33.6673346693387, Blast_Score=265, Evalue=5e-71,
Organism=Drosophila melanogaster, GI24656367, Length=513, Percent_Identity=30.2144249512671, Blast_Score=238, Evalue=9e-63,
Organism=Drosophila melanogaster, GI21357867, Length=525, Percent_Identity=29.3333333333333, Blast_Score=235, Evalue=7e-62,
Organism=Drosophila melanogaster, GI24656372, Length=367, Percent_Identity=29.700272479564, Blast_Score=171, Evalue=8e-43,
Organism=Drosophila melanogaster, GI45552909, Length=308, Percent_Identity=28.2467532467532, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 54710; Mature: 54710

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPRPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRVGLNGTSIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHEEEEECCCCCHHHHHHHHHCCC
SEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKLAFGTVDSWLVWNFTKHELHV
HHHHHHHCCCCHHHHHHHHEEEEEHHCCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
EECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCEEE
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESQNNLVTTIAWQVDGKVNYA
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCEEEEE
LEGSIFIAGAVVQWLRDGLGIIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGIRIGELRVDGGASANNLLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPRVTETTALGAAYLAGLAIGYWKNVDELHSQWQLERRFAPSMQREQVSS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
CLCGWQRAVRAAKAWADDTQ
HHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPRPGWVEHDPQEIWSTQAGVA
CCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCCCCCHH
AEAVTRVGLNGTSIAAIGITNQRETTIVWDRETGHPIYNAIVWQDRRTADFCDQLKAQGL
HHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCCHHEEEEECCCCCHHHHHHHHHCCC
SEKVRAKTGLPIDSYFSATKIRWILDNVEGAREKARQGKLAFGTVDSWLVWNFTKHELHV
HHHHHHHCCCCHHHHHHHHEEEEEHHCCHHHHHHHHCCCEEEECCCCEEEEEEECCEEEE
TDVTNASRTMLFNIHTLDWDDELLDALEIPRSMLPQVRASSEIYGPTKTTVFASKIPLAG
EECCCCCEEEEEEEEECCCCHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEECCCCEEE
IAGDQQAALFGQMCTSSGMVKNTYGTGCFLMMNTGETPIESQNNLVTTIAWQVDGKVNYA
CCCCCHHHHHHHHHHCCCCEEECCCCEEEEEEECCCCCCCCCCCEEEEEEEEECCEEEEE
LEGSIFIAGAVVQWLRDGLGIIKSASEIEALAAGVPHTDGVYLVPAFAGLGAPHWNARAR
ECCCEEEHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCC
GSLFGVTRGTTSAHLARAALDSIAYQSLDVLKAMEADSGIRIGELRVDGGASANNLLMQF
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHHHHH
QADLLGVDAVRPRVTETTALGAAYLAGLAIGYWKNVDELHSQWQLERRFAPSMQREQVSS
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHH
CLCGWQRAVRAAKAWADDTQ
HHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA