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The map label for this gene is glpF [H]

Identifier: 83719155

GI number: 83719155

Start: 681551

End: 682267

Strand: Reverse

Name: glpF [H]

Synonym: BTH_I0598

Alternate gene names: 83719155

Gene position: 682267-681551 (Counterclockwise)

Preceding gene: 83719921

Following gene: 83721343

Centisome position: 17.91

GC content: 67.92

Gene sequence:

>717_bases
ATGTCACCATACATCGCGGAGTTCATCGGCACGGCCCTCCTCGTGCTGCTCGGCAACGGCGCCGTCGCGAACGTCCTGCT
CGCGAAGACCAAAGGCAAAGGCGCGGACCTCATCGTCATCGTGATGGGTTGGGCGATGGCGGTATTCGTCGCGGTCTACG
TGACCGCATCGTTCTCCGGCGCGCACCTGAATCCGATCGTCACGATCAGCCTCGCGCTCGCCGGCAAGTTCGCCTGGGCG
AAGGTGGGCGGCTACATCGCGGCGCAGATGCTGGGCGGCATGGCGGGCGCGCTCCTCGTGTGGCTTGCATATCGCCAGCA
CTTCGCGAAGGAAACCGATCCCGACCTGAAGCTCGCCGTGTTCTGCACGGCGCCCGCGATCCGCAGCGTCACGCACAACG
TGCTGACGGAGGCGATCTGCACGTTCGTGCTGATCCTCGGCGTGCTGTATCTCGCGTCGCCGCAAGTCGGTCTCGGCGCG
CTCGACGCGCTGCCCGTCGGCCTGCTCGTGCTCGGCATCGGCATCTCGCTCGGCGGCCCGACGGGCTACGCGATGAGCCC
CGCGCGCGACCTGTCGCCGCGCATCATGCACGCGCTGCTGCCGATTCCCGGCAAGCGCGACAGCGACTGGCGCTACGCAT
GGGTGCCGGTGCTCGGGCCGCTCGTCGGCGGCGTGCTCGCGGCGAACCTGTACCTGTATCTGCATACGGCGCACTGA

Upstream 100 bases:

>100_bases
GCATCCGTATCGATCTCATTTCAACGACACCATTCACAACAATCAGGCGGGCCGCGCGACGCAAGCCGCGAGCCCGCCAT
ACCGGTAGAGAGACAACATC

Downstream 100 bases:

>100_bases
ACACCGCGCGTCGAATCGCCGGCCGCACGCGGGCGGCGCTCTTCCCCCCCCTCTGCCGGCAGCGCACGAGGGGGGGTCGT
TTTCGGCGCGCGTGCCGGCA

Product: glycerol uptake facilitator protein

Products: glycerol [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 238; Mature: 237

Protein sequence:

>238_residues
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWA
KVGGYIAAQMLGGMAGALLVWLAYRQHFAKETDPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTAH

Sequences:

>Translated_238_residues
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWA
KVGGYIAAQMLGGMAGALLVWLAYRQHFAKETDPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGA
LDALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTAH
>Mature_237_residues
SPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSGAHLNPIVTISLALAGKFAWAK
VGGYIAAQMLGGMAGALLVWLAYRQHFAKETDPDLKLAVFCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGAL
DALPVGLLVLGIGISLGGPTGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTAH

Specific function: Glycerol enters the cell via the glycerol diffusion facilitator protein. This membrane protein facilitates the movement of glycerol across the cytoplasmic membrane [H]

COG id: COG0580

COG function: function code G; Glycerol uptake facilitator and related permeases (Major Intrinsic Protein Family)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MIP/aquaporin (TC 1.A.8) family [H]

Homologues:

Organism=Homo sapiens, GI157266307, Length=248, Percent_Identity=33.4677419354839, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI4826645, Length=245, Percent_Identity=32.6530612244898, Blast_Score=106, Evalue=2e-23,
Organism=Homo sapiens, GI22538420, Length=248, Percent_Identity=31.8548387096774, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI4502187, Length=241, Percent_Identity=33.195020746888, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI4502181, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=9e-13,
Organism=Homo sapiens, GI4755125, Length=235, Percent_Identity=27.2340425531915, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4502179, Length=235, Percent_Identity=30.2127659574468, Blast_Score=69, Evalue=3e-12,
Organism=Escherichia coli, GI1790362, Length=252, Percent_Identity=35.3174603174603, Blast_Score=102, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI71994009, Length=243, Percent_Identity=31.2757201646091, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI17544068, Length=248, Percent_Identity=31.4516129032258, Blast_Score=97, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17531431, Length=243, Percent_Identity=27.5720164609054, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17531429, Length=243, Percent_Identity=27.5720164609054, Blast_Score=86, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17558372, Length=238, Percent_Identity=29.8319327731092, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71992966, Length=238, Percent_Identity=27.3109243697479, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321054, Length=211, Percent_Identity=32.7014218009479, Blast_Score=94, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6322985, Length=279, Percent_Identity=26.8817204301075, Blast_Score=79, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24762342, Length=236, Percent_Identity=26.6949152542373, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI19922828, Length=231, Percent_Identity=30.3030303030303, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24652747, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI45551084, Length=232, Percent_Identity=26.2931034482759, Blast_Score=64, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012269
- InterPro:   IPR000425
- InterPro:   IPR022357 [H]

Pfam domain/function: PF00230 MIP [H]

EC number: NA

Molecular weight: Translated: 24873; Mature: 24742

Theoretical pI: Translated: 9.29; Mature: 9.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWAKVGGYIAAQMLGGMAGALLVWLAYRQHFAKETDPDLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTAH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEEEEECC
>Mature Secondary Structure 
SPYIAEFIGTALLVLLGNGAVANVLLAKTKGKGADLIVIVMGWAMAVFVAVYVTASFSG
CCHHHHHHHHHHHHHHCCCHHHHHEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHCCCC
AHLNPIVTISLALAGKFAWAKVGGYIAAQMLGGMAGALLVWLAYRQHFAKETDPDLKLAV
CCCCHHEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEE
FCTAPAIRSVTHNVLTEAICTFVLILGVLYLASPQVGLGALDALPVGLLVLGIGISLGGP
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCC
TGYAMSPARDLSPRIMHALLPIPGKRDSDWRYAWVPVLGPLVGGVLAANLYLYLHTAH
CCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: glycerol [Periplasm] [C]

Specific reaction: glycerol [Periplasm] = glycerol [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]