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The map label for this gene is gudD [H]

Identifier: 83719461

GI number: 83719461

Start: 219483

End: 220856

Strand: Direct

Name: gudD [H]

Synonym: BTH_I0189

Alternate gene names: 83719461

Gene position: 219483-220856 (Clockwise)

Preceding gene: 83720349

Following gene: 83719740

Centisome position: 5.76

GC content: 69.43

Gene sequence:

>1374_bases
ATGAACGCTTTGATGGAGACACTTGTAATGCGAACCGAATTGAGCCGTACGACGACGCCCGTCGTGTCCGACCTGCGCGT
CGTGCCCGTGGCCGGACGCGACAGCATGCTGCTGAACCTGAGCGGCGCGCACGGGCCCTTCTTCACGCGCAACATCGTGC
TGATCCGCGACAGCGCGGGCAATACCGGCGTCGGCGAAGTGCCGGGCGGCGAAGGCATCCGGCGCACGCTCGAGGATGCG
CGCGCGCTCGTCGTCGGCCAGCCGCTCGGCCGCTGGCAGGATGTGCTGAACGCGGTGCGCGCCCGCTTCGCCGATCGCGA
TGCGGGCGGCCGCGGCTTGCAGACCTTCGACCTGCGCATCACGATTCACGCGGTGACGGCGCTCGAGGCGGCGCTGCTCG
ACCTGCTCGGCCAGCACCTCGGCGTGCCCGTCGCCGCGCTGCTCGGCGAAGGCCAGCAGCGCAGCCGCGTGCCGATGCTC
GGCTATCTGTTCTACATCGGCGATCGCAAGCGCACCGATCTCGACTACCGCAGCGAGCCGCAGGCCGACGACGCGTGGTT
CCGTCTGCGCAACGAAGTGGCGCTCACGCCCGATGCGATCGTGCGCCTTGCCGAGGCCGCGCACGCGCGCTACGGATTTC
AGGATTTCAAGCTGAAGGGCGGCGTGCTGTCGGGCGATGACGAAATCGCCGCGGTGACCGCGCTCGCGAAGCGCTTCCCG
AACGCGCGGATCACGCTCGATCCGAACGGCGCATGGTCGCTGCAGGAGGCGATTCGCCTCTGCCGCGGCAAGGGCGACGT
GCTCGCGTACGCGGAAGATCCGTGCGGCGCGGAGAACGGCTACTCGGGCCGCGAGATCATGGCCGAGTTTCGCCGCGCGA
CGGGCCTGCCGACGGCGACCAACATGATCGCGACCGACTGGCGCCAGCTCGGCCACGCGCTCCGGCTCGGCTCGGTCGAC
ATTCCGCTCGCCGATCCGCATTTCTGGACGATGCGCGGCTCGGTGCGCGTCGCGCAACTGTGCGACGAATGGGGGCTCAC
GTGGGGCTCGCATTCGAACAATCACTTCGACATTTCGCTCGCGATGTTCACGCACGTCGCGGCCGCCGCGCCCGGCAACA
TCACCGCGATCGACACGCACTGGATCTGGCAGGACGGCCAGCGCCTGACGCGCGAGCCGCTGCGCATCGAGGACGGCCAC
GTCGCGGTGCCGCAGCGTGGCGGGCTCGGCGTCGAGATCGACGAGGACGCGCTCGCCGGCGCACACGAGCTGTATCTGGA
GCACGGCCTCGGCAGCCGCGACGATGCGGTCGCGATGCGGTACCTGGTGCCGGACTGGACGTTCGACAACAAGCGCCCCT
GTCTCGTGCGCTGA

Upstream 100 bases:

>100_bases
GCGACGACGCGCGGGCGATGCAATATCGGGTGCCAGGCTGGGCCTACCACCCGAAACGGCCGAGCTTCGGCAGCGCTGCG
CGCGCAGCCGCGCGCGGCGC

Downstream 100 bases:

>100_bases
ACGAAGCCGACTCTTCATGGAGCGCTCGATGCGGGAGCCCACATCGCCCGACGGCACGCGCACGGAGCGCGCGCTGACGA
TTCGCGTGCACGAAAGCGAC

Product: glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNALMETLVMRTELSRTTTPVVSDLRVVPVAGRDSMLLNLSGAHGPFFTRNIVLIRDSAGNTGVGEVPGGEGIRRTLEDA
RALVVGQPLGRWQDVLNAVRARFADRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRSRVPML
GYLFYIGDRKRTDLDYRSEPQADDAWFRLRNEVALTPDAIVRLAEAAHARYGFQDFKLKGGVLSGDDEIAAVTALAKRFP
NARITLDPNGAWSLQEAIRLCRGKGDVLAYAEDPCGAENGYSGREIMAEFRRATGLPTATNMIATDWRQLGHALRLGSVD
IPLADPHFWTMRGSVRVAQLCDEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPLRIEDGH
VAVPQRGGLGVEIDEDALAGAHELYLEHGLGSRDDAVAMRYLVPDWTFDNKRPCLVR

Sequences:

>Translated_457_residues
MNALMETLVMRTELSRTTTPVVSDLRVVPVAGRDSMLLNLSGAHGPFFTRNIVLIRDSAGNTGVGEVPGGEGIRRTLEDA
RALVVGQPLGRWQDVLNAVRARFADRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRSRVPML
GYLFYIGDRKRTDLDYRSEPQADDAWFRLRNEVALTPDAIVRLAEAAHARYGFQDFKLKGGVLSGDDEIAAVTALAKRFP
NARITLDPNGAWSLQEAIRLCRGKGDVLAYAEDPCGAENGYSGREIMAEFRRATGLPTATNMIATDWRQLGHALRLGSVD
IPLADPHFWTMRGSVRVAQLCDEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPLRIEDGH
VAVPQRGGLGVEIDEDALAGAHELYLEHGLGSRDDAVAMRYLVPDWTFDNKRPCLVR
>Mature_457_residues
MNALMETLVMRTELSRTTTPVVSDLRVVPVAGRDSMLLNLSGAHGPFFTRNIVLIRDSAGNTGVGEVPGGEGIRRTLEDA
RALVVGQPLGRWQDVLNAVRARFADRDAGGRGLQTFDLRITIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRSRVPML
GYLFYIGDRKRTDLDYRSEPQADDAWFRLRNEVALTPDAIVRLAEAAHARYGFQDFKLKGGVLSGDDEIAAVTALAKRFP
NARITLDPNGAWSLQEAIRLCRGKGDVLAYAEDPCGAENGYSGREIMAEFRRATGLPTATNMIATDWRQLGHALRLGSVD
IPLADPHFWTMRGSVRVAQLCDEWGLTWGSHSNNHFDISLAMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPLRIEDGH
VAVPQRGGLGVEIDEDALAGAHELYLEHGLGSRDDAVAMRYLVPDWTFDNKRPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=444, Percent_Identity=72.972972972973, Blast_Score=691, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=447, Percent_Identity=62.1923937360179, Blast_Score=548, Evalue=1e-157,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 50134; Mature: 50134

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALMETLVMRTELSRTTTPVVSDLRVVPVAGRDSMLLNLSGAHGPFFTRNIVLIRDSAG
CHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCEEEEECCCCCCEEEEEEEEEEECCC
NTGVGEVPGGEGIRRTLEDARALVVGQPLGRWQDVLNAVRARFADRDAGGRGLQTFDLRI
CCCCCCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
TIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRSRVPMLGYLFYIGDRKRTDLDYRSEP
EEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCC
QADDAWFRLRNEVALTPDAIVRLAEAAHARYGFQDFKLKGGVLSGDDEIAAVTALAKRFP
CCCHHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHHCC
NARITLDPNGAWSLQEAIRLCRGKGDVLAYAEDPCGAENGYSGREIMAEFRRATGLPTAT
CCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
NMIATDWRQLGHALRLGSVDIPLADPHFWTMRGSVRVAQLCDEWGLTWGSHSNNHFDISL
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEH
AMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPLRIEDGHVAVPQRGGLGVEIDEDALAG
HHHHHHHHCCCCCEEEEEEEEEECCCHHHCCCCCEECCCEEECCCCCCCCEEECHHHHCC
AHELYLEHGLGSRDDAVAMRYLVPDWTFDNKRPCLVR
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MNALMETLVMRTELSRTTTPVVSDLRVVPVAGRDSMLLNLSGAHGPFFTRNIVLIRDSAG
CHHHHHHHHHHHHHHHCCCCHHHCCEEEEECCCCCEEEEECCCCCCEEEEEEEEEEECCC
NTGVGEVPGGEGIRRTLEDARALVVGQPLGRWQDVLNAVRARFADRDAGGRGLQTFDLRI
CCCCCCCCCCHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEE
TIHAVTALEAALLDLLGQHLGVPVAALLGEGQQRSRVPMLGYLFYIGDRKRTDLDYRSEP
EEEHHHHHHHHHHHHHHHHHCCCHHHHHCCCCHHCCCCEEEEEEEECCCCCCCCCCCCCC
QADDAWFRLRNEVALTPDAIVRLAEAAHARYGFQDFKLKGGVLSGDDEIAAVTALAKRFP
CCCHHHHHHCCCEEECHHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHHCC
NARITLDPNGAWSLQEAIRLCRGKGDVLAYAEDPCGAENGYSGREIMAEFRRATGLPTAT
CCEEEECCCCCCCHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCHH
NMIATDWRQLGHALRLGSVDIPLADPHFWTMRGSVRVAQLCDEWGLTWGSHSNNHFDISL
HHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCEEEEEH
AMFTHVAAAAPGNITAIDTHWIWQDGQRLTREPLRIEDGHVAVPQRGGLGVEIDEDALAG
HHHHHHHHCCCCCEEEEEEEEEECCCHHHCCCCCEECCCEEECCCCCCCCEEECHHHHCC
AHELYLEHGLGSRDDAVAMRYLVPDWTFDNKRPCLVR
HHHHHHHHCCCCCCCCEEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]