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The map label for this gene is gudD [H]

Identifier: 83720349

GI number: 83720349

Start: 219016

End: 219486

Strand: Direct

Name: gudD [H]

Synonym: BTH_I0188

Alternate gene names: 83720349

Gene position: 219016-219486 (Clockwise)

Preceding gene: 83721570

Following gene: 83719461

Centisome position: 5.75

GC content: 70.49

Gene sequence:

>471_bases
ATGTCGAACATGACGTCGAATGCGATCGAAGCCGCCGCGCAAGCGCACGCGAACACGCACACGCCGAGCCCGAGCGTCGC
GCAACGGTGCGACGAATGGGGGCGCACATGGGGCTCGCATTCGAACCATCACTTCGACATTTCGCTCGCGATGTTCACGC
ACGTCGCGGCCGCCGCGCCCGGCAACATCACCGCGATCGACGCGCACTGGATCTGGCAGGAAGCCGACGAGCGCCTGACG
CGCGAGCCGCTCGCGATCGCGCGCGGGCACGTCGCGGTGCCGGAGGGACCCGGGCTCGGCATCGCGCTCGACATGGACCG
CGTGATGCAGGCCCATGCGCTGTATGAGACGCTCGGTCCGGGCGCGCGCGACGACGCGCGGGCGATGCAATATCGGGTGC
CAGGCTGGGCCTACCACCCGAAACGGCCGAGCTTCGGCAGCGCTGCGCGCGCAGCCGCGCGCGGCGCATGA

Upstream 100 bases:

>100_bases
CCTACGTGGGGCTTCATGCGCTCGTCGCGTGCGTCTGCTACCTGTTCGTGGTCGGCGAGATCAAGCGCGTCGAGCTGAAG
CCGGCATCGTGAAGCGAGGC

Downstream 100 bases:

>100_bases
ACGCTTTGATGGAGACACTTGTAATGCGAACCGAATTGAGCCGTACGACGACGCCCGTCGTGTCCGACCTGCGCGTCGTG
CCCGTGGCCGGACGCGACAG

Product: hypothetical protein

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 156; Mature: 155

Protein sequence:

>156_residues
MSNMTSNAIEAAAQAHANTHTPSPSVAQRCDEWGRTWGSHSNHHFDISLAMFTHVAAAAPGNITAIDAHWIWQEADERLT
REPLAIARGHVAVPEGPGLGIALDMDRVMQAHALYETLGPGARDDARAMQYRVPGWAYHPKRPSFGSAARAAARGA

Sequences:

>Translated_156_residues
MSNMTSNAIEAAAQAHANTHTPSPSVAQRCDEWGRTWGSHSNHHFDISLAMFTHVAAAAPGNITAIDAHWIWQEADERLT
REPLAIARGHVAVPEGPGLGIALDMDRVMQAHALYETLGPGARDDARAMQYRVPGWAYHPKRPSFGSAARAAARGA
>Mature_155_residues
SNMTSNAIEAAAQAHANTHTPSPSVAQRCDEWGRTWGSHSNHHFDISLAMFTHVAAAAPGNITAIDAHWIWQEADERLTR
EPLAIARGHVAVPEGPGLGIALDMDRVMQAHALYETLGPGARDDARAMQYRVPGWAYHPKRPSFGSAARAAARGA

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc). Also acts on L-idarate [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=133, Percent_Identity=64.6616541353383, Blast_Score=184, Evalue=2e-48,
Organism=Escherichia coli, GI1789151, Length=141, Percent_Identity=59.5744680851064, Blast_Score=175, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 16811; Mature: 16680

Theoretical pI: Translated: 7.09; Mature: 7.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNMTSNAIEAAAQAHANTHTPSPSVAQRCDEWGRTWGSHSNHHFDISLAMFTHVAAAAP
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCC
GNITAIDAHWIWQEADERLTREPLAIARGHVAVPEGPGLGIALDMDRVMQAHALYETLGP
CCEEEEEHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCCEEEECHHHHHHHHHHHHHHCC
GARDDARAMQYRVPGWAYHPKRPSFGSAARAAARGA
CCCCCHHHHEEECCCCEECCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SNMTSNAIEAAAQAHANTHTPSPSVAQRCDEWGRTWGSHSNHHFDISLAMFTHVAAAAP
CCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHCCC
GNITAIDAHWIWQEADERLTREPLAIARGHVAVPEGPGLGIALDMDRVMQAHALYETLGP
CCEEEEEHHHHHHHHHHHHHHCCHHHHCCCEECCCCCCCEEEECHHHHHHHHHHHHHHCC
GARDDARAMQYRVPGWAYHPKRPSFGSAARAAARGA
CCCCCHHHHEEECCCCEECCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]