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The map label for this gene is metH [H]

Identifier: 83719356

GI number: 83719356

Start: 396816

End: 399533

Strand: Reverse

Name: metH [H]

Synonym: BTH_I0357

Alternate gene names: 83719356

Gene position: 399533-396816 (Counterclockwise)

Preceding gene: 83720125

Following gene: 83721308

Centisome position: 10.49

GC content: 65.23

Gene sequence:

>2718_bases
ATGACCGACCATACGATGCGCCTAGCCGGCCTCGAGCCGTTCAACGTCACGTCCGGGACGCTCTTCATCAACGTCGGCGA
ACGCACCAACGTCACCGGCTCGAAGGCGTTCGCGCGGATGATCCTCAACGGCCAGTTCGACGAGGCGCTCGCCGTCGCGC
GCCAGCAGGTCGAGAACGGCGCGCAGGTGATCGACATCAACATGGACGAGGCGATGCTCGATTCGAAGGCGGCGATGGTG
CGCTTCCTGAATCTGATCGCGTCGGAGCCGGACATCGCGCGCGTGCCGATCATGATCGACTCGTCGAAGTGGGACGTGAT
CGAAGCGGGCCTGAAGTGCGTGCAGGGCAAGGCGATCGTCAACTCGATCTCGCTGAAGGAAGGCGAGGAGGCGTTCGTGC
ATCACGCGAAGCTGATCCGCCGCTACGGCGCGGCGAGCGTCGTGATGGCGTTCGACGAGCAGGGCCAGGCCGACACGTTC
GCCCGCAAGACTGAAATCTGCAAGCGCTCGTACGACGTGCTCGTGAACCAATGCGGCTTCCCGCCCGAGGACATCATCTT
CGACCCGAACATCTTCGCGGTCGCCACCGGCATCGAGGAGCACAACAACTACGCGGTCGACTTCATCGAGGCGACCCGCT
GGATCAAGCAGAACCTGCCGTACGCGAAGGTGAGCGGCGGCGTGTCGAACGTGTCGTTCTCGTTTCGCGGCAACGATCCC
GTGCGCGAGGCGATCCACACCGTGTTCCTCTATCACGCGATCCAGGCGGGGATGGACATGGGCATCGTCAACGCGGGCCA
GCTCGGCGTGTACGCGGAACTCGACGCCGAGCTGCGCGAGCGCGTCGAGGACGTGATCCTGAACCGCCGCGCCGATTCGA
CCGACCGCCTGCTGGAGATCGCCGACAAGTTCAAGACGGGCGCCGCGAAGAAGGAAGAGAACCTCGAGTGGCGCAACCAG
CCGGTCGAGAAGCGGCTCGCGCACGCGCTCGTGCACGGGATCACGAACTTTATCGTCGAGGATACGGAAGAAGCGCGCGC
GAAGATCGCCGCGGCCGGCGGCCGGCCGATCAACGTGATCGAAGGCCCGCTGATGGACGGAATGAACGTCGTCGGCGACC
TGTTCGGCCAGGGCAAGATGTTCCTGCCGCAAGTCGTGAAATCGGCGCGCGTGATGAAGCAGGCGGTCGCGCACCTGATC
CCGTTCATCGAGGAAGAAAAGCGCCTGCTCGCGGAGGCGGGCGGCGACGTGCGCGCGAAGGGCAAGATCGTGATCGCGAC
CGTGAAGGGCGACGTGCACGACATCGGCAAGAACATCGTGTCGGTCGTGCTCCAGTGCAACAACTTCGAAGTGGTCAACA
TGGGCGTGATGGTGCCGTGCAACGAGATCCTCGCGAAGGCGAAGGTCGAGGGCGCGGACATCGTCGGGCTGTCGGGCCTC
ATCACGCCGAGCCTCGAGGAAATGGCCTACGTCGCCTCCGAGATGCAGCGCGACGACTACTTCCGCGTGAAGAAGATTCC
GCTCTTGATCGGCGGCGCGACCACCTCGCGCGTGCACACCGCCGTGAAGATCGCGCCGAACTACGAAGGGCCCGTCGTTT
ATGTTCCGGACGCGTCGCGCTCGGTGTCGGTCGCCTCGAGCCTGCTGTCCGACGAAGGCGCGGCCAAGTACCTCGACGAG
CTGAAATCCGATTACGAGCGCATCCGCACGCAGCACGCCAACAAGAAGGCGCTGCCGCTCGTCACGCTCGACGAAGCGCG
CGCGAACAAGACCAAGGTCGACTGGGCGAGCTACCAGCCGGTGAAGCCGAAGTTCATCGGCCGCCGCGTGTTCAAGAACT
ACGATCTGAACGAGCTCGCGAACTACATCGATTGGGGCCCGTTCTTCCAGACCTGGGATCTCGCGGGTCCGTACCCGGCG
ATCCTGAACGACGAGATCGTCGGCGAATCGGCGCGCCGCGTGTTCTCCGACGCGAAATCGATGCTCGCACGCCTGATCCA
GGGCCGCTGGCTGACCGCGAACGGCGTGATCGCGCTGCTGCCGGCGAACACGGTCGGCGACGACGACATCGAGATCTACA
CCGACGACACGCGCTCGGAAGTGCTGCTCACGTGGCGCAACCTGCGCCAGCAGAGCGTGCGTCCGGTGGTGGACGGCGTG
ATGCGGCCGAACCGGTCGCTCGCCGACTTCATCGCGCCGAAGGATTCGGGCGTCGCCGACTATATCGGGATGTTCGCGGT
GACGGCCGGGATCGGCGTCGACGCGAAGGAAAAGCAGTTCCTCGCCGATCACGACGATTACAGCGCGATCATGCTGAAGG
CGCTCGCCGACCGGCTCGCGGAAGCGTTCGCCGAGGCGATGCACGCGCGCGTGCGCCGCGAGCTGTGGGGCTATGCGGCG
GGCGAGCAGCTCGACAACGACGCGCTGATCGCCGAGAAATACGCGGGCATCCGCCCGGCGCCCGGCTACCCGGCATGCCC
GGACCACCTCGTGAAGCGCGCAATGTTCGAAGCGCTTCATACAGACGAGATCGGGATGAGCATCACCGATTCGCTCGCGA
TGCTGCCGGCCGCGAGCGTCTCCGGCTTCTATCTCGCGCATCCGGACAGCACGTACTTCTCGGTCGGCAAGATCAGCCAG
GACCAGCTCGAGGATTTCGCGCGCCGGATGGCGCTGTCGAAGGCGGACGCCGAGCGCGCGCTCGCGCCGCTGCTCTGA

Upstream 100 bases:

>100_bases
GCTCGCCGAGGTCAAGCCGCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGACGCGCGCCGCCCTCCCCACTCCAAC
CCGAATACGCACGCACCGCC

Downstream 100 bases:

>100_bases
CGGACAGTGCGCGCGCGGCGGCCGTGCGACGGCATGCGCCGGGGCCGGCGGCGGGGCTCGCCCGGAGCCGGAGCCGGAAC
CGCCGATGGCGACGACGGCC

Product: 5-methyltetrahydrofolate--homocysteine methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 905; Mature: 904

Protein sequence:

>905_residues
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEAFVHHAKLIRRYGAASVVMAFDEQGQADTF
ARKTEICKRSYDVLVNQCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRADSTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE
LKSDYERIRTQHANKKALPLVTLDEARANKTKVDWASYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRSEVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAA
GEQLDNDALIAEKYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSITDSLAMLPAASVSGFYLAHPDSTYFSVGKISQ
DQLEDFARRMALSKADAERALAPLL

Sequences:

>Translated_905_residues
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMV
RFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEAFVHHAKLIRRYGAASVVMAFDEQGQADTF
ARKTEICKRSYDVLVNQCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRADSTDRLLEIADKFKTGAAKKEENLEWRNQ
PVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLI
PFIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGL
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDE
LKSDYERIRTQHANKKALPLVTLDEARANKTKVDWASYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPA
ILNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRSEVLLTWRNLRQQSVRPVVDGV
MRPNRSLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAA
GEQLDNDALIAEKYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSITDSLAMLPAASVSGFYLAHPDSTYFSVGKISQ
DQLEDFARRMALSKADAERALAPLL
>Mature_904_residues
TDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENGAQVIDINMDEAMLDSKAAMVR
FLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIVNSISLKEGEEAFVHHAKLIRRYGAASVVMAFDEQGQADTFA
RKTEICKRSYDVLVNQCGFPPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDPV
REAIHTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRADSTDRLLEIADKFKTGAAKKEENLEWRNQP
VEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVIEGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIP
FIEEEKRLLAEAGGDVRAKGKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGLI
TPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASRSVSVASSLLSDEGAAKYLDEL
KSDYERIRTQHANKKALPLVTLDEARANKTKVDWASYQPVKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAI
LNDEIVGESARRVFSDAKSMLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRSEVLLTWRNLRQQSVRPVVDGVM
RPNRSLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLAEAFAEAMHARVRRELWGYAAG
EQLDNDALIAEKYAGIRPAPGYPACPDHLVKRAMFEALHTDEIGMSITDSLAMLPAASVSGFYLAHPDSTYFSVGKISQD
QLEDFARRMALSKADAERALAPLL

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=918, Percent_Identity=54.5751633986928, Blast_Score=976, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=904, Percent_Identity=62.8318584070796, Blast_Score=1113, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=912, Percent_Identity=54.2763157894737, Blast_Score=946, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 99526; Mature: 99395

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEEAFVHHAKLIRRYGAASVVMAFDEQGQADTFARKTEICKRSYDVLVNQCGF
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRADSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEE
EGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEECCCCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGAAKYLDELKSDYERIRTQHANKKALPLVTLDEARANKTKVDWASYQP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRSEVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLA
HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYAAGEQLDNDALIAEKYAGIRPAPGYPACPDHLVKRAMFEALH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
TDEIGMSITDSLAMLPAASVSGFYLAHPDSTYFSVGKISQDQLEDFARRMALSKADAERA
HCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LAPLL
HCCCC
>Mature Secondary Structure 
TDHTMRLAGLEPFNVTSGTLFINVGERTNVTGSKAFARMILNGQFDEALAVARQQVENG
CCCCEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHCC
AQVIDINMDEAMLDSKAAMVRFLNLIASEPDIARVPIMIDSSKWDVIEAGLKCVQGKAIV
CEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHCCHHHH
NSISLKEGEEAFVHHAKLIRRYGAASVVMAFDEQGQADTFARKTEICKRSYDVLVNQCGF
HHCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCC
PPEDIIFDPNIFAVATGIEEHNNYAVDFIEATRWIKQNLPYAKVSGGVSNVSFSFRGNDP
CCHHEEECCCCEEEEECCHHCCCEEEHHHHHHHHHHHCCCCEEECCCCCEEEEEECCCCH
VREAIHTVFLYHAIQAGMDMGIVNAGQLGVYAELDAELRERVEDVILNRRADSTDRLLEI
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHCCCCCCHHHHHHH
ADKFKTGAAKKEENLEWRNQPVEKRLAHALVHGITNFIVEDTEEARAKIAAAGGRPINVI
HHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCEEEE
EGPLMDGMNVVGDLFGQGKMFLPQVVKSARVMKQAVAHLIPFIEEEKRLLAEAGGDVRAK
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEC
GKIVIATVKGDVHDIGKNIVSVVLQCNNFEVVNMGVMVPCNEILAKAKVEGADIVGLSGL
CEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEEEEECCHHHHHHHHHCCCCCEECCCCC
ITPSLEEMAYVASEMQRDDYFRVKKIPLLIGGATTSRVHTAVKIAPNYEGPVVYVPDASR
CCCCHHHHHHHHHHHCCCCCEEEEECEEEEECCCHHHEEEEEEECCCCCCCEEECCCCCC
SVSVASSLLSDEGAAKYLDELKSDYERIRTQHANKKALPLVTLDEARANKTKVDWASYQP
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCC
VKPKFIGRRVFKNYDLNELANYIDWGPFFQTWDLAGPYPAILNDEIVGESARRVFSDAKS
CCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHH
MLARLIQGRWLTANGVIALLPANTVGDDDIEIYTDDTRSEVLLTWRNLRQQSVRPVVDGV
HHHHHHCCCEEECCCEEEEEECCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
MRPNRSLADFIAPKDSGVADYIGMFAVTAGIGVDAKEKQFLADHDDYSAIMLKALADRLA
HCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
EAFAEAMHARVRRELWGYAAGEQLDNDALIAEKYAGIRPAPGYPACPDHLVKRAMFEALH
HHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
TDEIGMSITDSLAMLPAASVSGFYLAHPDSTYFSVGKISQDQLEDFARRMALSKADAERA
HCCCCCHHHHHHHHCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
LAPLL
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]