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The map label for this gene is metH [H]

Identifier: 83720125

GI number: 83720125

Start: 399579

End: 400655

Strand: Reverse

Name: metH [H]

Synonym: BTH_I0358

Alternate gene names: 83720125

Gene position: 400655-399579 (Counterclockwise)

Preceding gene: 83718697

Following gene: 83719356

Centisome position: 10.52

GC content: 68.71

Gene sequence:

>1077_bases
ATGTCCGAGCCCAAGCCGATCGCGCCTTCCGCCCCCTCCGCCCTCGCCGCGTCGTACACGCGCGGCGCGGCGCTGCCGCG
GCTGCTCCAGCAGCGCATCCTGATCCTCGACGGCGCGATGGGCACGATGATCCAGCGCTACAAGCTCGACGAGGCCGCGT
ATCGCAGCGAGCGCTTCAAGGATTTTCCGCGCGACGTGAAGGGCAACAACGAGCTCTTGTCGATCACGCAGCCGCAGATC
ATCCGCGAGATCCACGACCAGTACTTCGCCGCCGGCGCCGACATCGTCGAGACGAACACGTTCGGCGCGACGGCCGTCGC
GCAGGCCGACTACGGAATGGAAGACCTTGCCGTCGAGATGAACGTCGCGTCGGCCACGCTCGCGCGCGAATCGGCCGCCA
AATACGCGACGCCCGACAAGCCGCGCTTCGTCGCGGGCGCGATCGGGCCGACGCCGAAGACGGCGAGCATCTCGCCCGAC
GTGAACGACCCGGGCGCGCGCAACGTCACGTTCGACGAGCTGCGCGACGCGTACTACACGCAGGCGAAGGCGCTCCTCGA
CGGCGGCGTCGATCTGTTCCTCGTCGAGACGATCTTCGACACGCTGAACGCGAAGGCCGCGCTCTTCGCGCTCGACCAGT
TGTTCGAGGACACCGGCGAACGGCTGCCGATCATGATCTCGGGCACCGTCACCGATGCGTCCGGGCGCATCCTGTCCGGC
CAGACGGTCGAGGCGTTCTGGAATTCGCTGCGCCACGCGAGGCCGCTCACGTTCGGCCTGAACTGCGCGCTCGGCGCGGC
GCTGATGCGCCCGTACATCGCCGAGCTCGCGAAGCTGTGCGACACCTACGTGTCGTGCTATCCGAACGCCGGCCTGCCGA
ACCCGATGAGCGACACCGGCTTCGACGAGACGCCCGACGTGACGTCGGGCCTGTTGAAGGAATTCGCGCAGGCGGGCCTC
GTGAACGTCGCGGGCGGCTGCTGCGGCACGACGCCCGAACACATCGCGGCGATCGCGAAGGCGCTCGCCGAGGTCAAGCC
GCGCCGCTGGCCGAGCCAGTACAGCGAAGCCGCCTGA

Upstream 100 bases:

>100_bases
GGCCGCCGCGCGGGGGCTTCGTTTGTCCACGCGCGGCCGCCTCACGTACGACGGAAGCAGCGGCTACAATGACGGCTTTC
CGATTCGCCGGTAATCCGCC

Downstream 100 bases:

>100_bases
CGCGCGCCGCCCTCCCCACTCCAACCCGAATACGCACGCACCGCCATGACCGACCATACGATGCGCCTAGCCGGCCTCGA
GCCGTTCAACGTCACGTCCG

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MSEPKPIAPSAPSALAASYTRGAALPRLLQQRILILDGAMGTMIQRYKLDEAAYRSERFKDFPRDVKGNNELLSITQPQI
IREIHDQYFAAGADIVETNTFGATAVAQADYGMEDLAVEMNVASATLARESAAKYATPDKPRFVAGAIGPTPKTASISPD
VNDPGARNVTFDELRDAYYTQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSG
QTVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGL
VNVAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA

Sequences:

>Translated_358_residues
MSEPKPIAPSAPSALAASYTRGAALPRLLQQRILILDGAMGTMIQRYKLDEAAYRSERFKDFPRDVKGNNELLSITQPQI
IREIHDQYFAAGADIVETNTFGATAVAQADYGMEDLAVEMNVASATLARESAAKYATPDKPRFVAGAIGPTPKTASISPD
VNDPGARNVTFDELRDAYYTQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSG
QTVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGL
VNVAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
>Mature_357_residues
SEPKPIAPSAPSALAASYTRGAALPRLLQQRILILDGAMGTMIQRYKLDEAAYRSERFKDFPRDVKGNNELLSITQPQII
REIHDQYFAAGADIVETNTFGATAVAQADYGMEDLAVEMNVASATLARESAAKYATPDKPRFVAGAIGPTPKTASISPDV
NDPGARNVTFDELRDAYYTQAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSGQ
TVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTGFDETPDVTSGLLKEFAQAGLV
NVAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=326, Percent_Identity=58.8957055214724, Blast_Score=391, Evalue=1e-109,
Organism=Escherichia coli, GI1790450, Length=326, Percent_Identity=58.5889570552147, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI1786456, Length=323, Percent_Identity=25.077399380805, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17535405, Length=330, Percent_Identity=53.6363636363636, Blast_Score=356, Evalue=1e-98,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 38406; Mature: 38275

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEPKPIAPSAPSALAASYTRGAALPRLLQQRILILDGAMGTMIQRYKLDEAAYRSERFK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFPRDVKGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATAVAQADYGMEDLAVEM
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCHHHHEEEH
NVASATLARESAAKYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRDAYYT
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSG
HHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECC
QTVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTG
CHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FDETPDVTSGLLKEFAQAGLVNVAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCC
>Mature Secondary Structure 
SEPKPIAPSAPSALAASYTRGAALPRLLQQRILILDGAMGTMIQRYKLDEAAYRSERFK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFPRDVKGNNELLSITQPQIIREIHDQYFAAGADIVETNTFGATAVAQADYGMEDLAVEM
HCCCCCCCCCCEEECCCHHHHHHHHHHHHHCCCCEEECCCCCCHHHHHCCCCHHHHEEEH
NVASATLARESAAKYATPDKPRFVAGAIGPTPKTASISPDVNDPGARNVTFDELRDAYYT
HHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
QAKALLDGGVDLFLVETIFDTLNAKAALFALDQLFEDTGERLPIMISGTVTDASGRILSG
HHHHHHHCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCEECC
QTVEAFWNSLRHARPLTFGLNCALGAALMRPYIAELAKLCDTYVSCYPNAGLPNPMSDTG
CHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
FDETPDVTSGLLKEFAQAGLVNVAGGCCGTTPEHIAAIAKALAEVKPRRWPSQYSEAA
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]