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The map label for this gene is bioF [H]

Identifier: 83718450

GI number: 83718450

Start: 375333

End: 376517

Strand: Reverse

Name: bioF [H]

Synonym: BTH_I0339

Alternate gene names: 83718450

Gene position: 376517-375333 (Counterclockwise)

Preceding gene: 83719742

Following gene: 83721594

Centisome position: 9.88

GC content: 73.92

Gene sequence:

>1185_bases
ATGAATCTGCTCGCCACGCTCGAACGCGGCCTGGCCGACATCGACGCACAGGGGCTGCGCCGCCGCCGCCGCATCGCGGA
CACCGCATGCAGCGCGCACATGAGCGTCGACGGCCGCGAGATCGTCGGCTTCGCGAGCAACGACTACCTCGGGCTCGCCG
CGCATCCGCGCTTGATCGAAGCGTTCGCCGAGGGCGCGCGGCGCTACGGCGCGGGCAGCGGCGGCTCGCATCTGCTCGGC
GGCCACTCGCGCGCGCACGCGGCGCTCGAAGACGAGCTCGCCGCGTTCTCGGGCGGCTTCTCCGACGCGCCGCGCGCGCT
CTACTTCAGCACCGGCTACATGGCGAACCTCGCCGCGCTCACCGCGCTCGCGGGCCGCGGCGCGACGATCTTCTCCGACG
CGCTCAATCACGCGTCGCTGATCGACGGCACGCGGCTGTCGCGCGCGAACGTGCAGGTCTACCCGCACGGCGACGCCGAC
GCGCTCGACGCGCTCCTGCGCGCCTGCGACGCGCCGACGAAGCTGATCGTCTCCGACACCGTGTTCAGCATGGACGGCGA
CGTCGCACCGCTCGCGCGGCTCGTCGCGCTCGCCGAAGCGCACGGCGCATGGCTCGTCGTCGACGATGCGCACGGCTTCG
GCGTGCTCGGCCCTCAAGGCCGCGGCGCACTCGCCGCGCACGGGCTGCGCTCGCCGAACCTCGTCTACGTCGGCACGCTC
GGCAAGGCGGCGGGCGCCGCGGGCGCGTTTATCGTCGCGCACGAGACGGTGATCGAATGGCTCGTGCAGCGCGCGCGCAG
CTACATCTTCACGACGGCCGCGCCGCCGTCCGTCGCGTGCGCGGTGTCCGCGAGCCTCGCGGTGATCGCGAGCGACGAAG
GCGACGCGCGGCGCGCGCATCTCGCCGCGCTGATCGAGCGCACGCGAGCGATCCTGCGCGCGACGCCGTGGCAACCGGTC
GATTCGCACACGGCCGTGCAGCCGCTCGTGATCGGCAGCAACGAAGCGACGCTCGCCGCGATGGCCGCGCTCGACGCGCA
GGGCCTGTGGGTGCCCGCGATCCGCCCGCCGACGGTGCCGGCCGGCACGTCGCGGCTGCGGATCTCGCTGTCGGCCGCGC
ACTCGTTCGCCGATCTCGCGCGCCTCGAAGCGGCGCTCGTCACGCCGATCGAGGCCGCCGCATGA

Upstream 100 bases:

>100_bases
TCTATCTGATGCCGCCTTACATCCTCGCCGACGGCGAGATCGAATGGCTTGCATCGCGCACGCGCGCGACGCTCGACGCA
ACGCTTTCGGAGACTCACGC

Downstream 100 bases:

>100_bases
GCGCGCCGCTCTCGCTGTTCGTGACGGGCACCGACACCGAGATCGGCAAGACCTTCGTATCGACCGCGCTGCTGCACGGC
TTCGCGCGCGCGGGGCTGCG

Product: 8-amino-7-oxononanoate synthase

Products: NA

Alternate protein names: AONS; 7-keto-8-amino-pelargonic acid synthase; 7-KAP synthase; KAPA synthase; 8-amino-7-ketopelargonate synthase; L-alanine--pimeloyl-CoA ligase [H]

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MNLLATLERGLADIDAQGLRRRRRIADTACSAHMSVDGREIVGFASNDYLGLAAHPRLIEAFAEGARRYGAGSGGSHLLG
GHSRAHAALEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGTRLSRANVQVYPHGDAD
ALDALLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAEAHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGAAGAFIVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLAALIERTRAILRATPWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFADLARLEAALVTPIEAAA

Sequences:

>Translated_394_residues
MNLLATLERGLADIDAQGLRRRRRIADTACSAHMSVDGREIVGFASNDYLGLAAHPRLIEAFAEGARRYGAGSGGSHLLG
GHSRAHAALEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGTRLSRANVQVYPHGDAD
ALDALLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAEAHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGAAGAFIVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLAALIERTRAILRATPWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFADLARLEAALVTPIEAAA
>Mature_394_residues
MNLLATLERGLADIDAQGLRRRRRIADTACSAHMSVDGREIVGFASNDYLGLAAHPRLIEAFAEGARRYGAGSGGSHLLG
GHSRAHAALEDELAAFSGGFSDAPRALYFSTGYMANLAALTALAGRGATIFSDALNHASLIDGTRLSRANVQVYPHGDAD
ALDALLRACDAPTKLIVSDTVFSMDGDVAPLARLVALAEAHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
GKAAGAAGAFIVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAHLAALIERTRAILRATPWQPV
DSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVPAGTSRLRISLSAAHSFADLARLEAALVTPIEAAA

Specific function: Catalyzes the decarboxylative condensation of pimeloyl- CoA and L-alanine to produce 8-amino-7-oxononanoate (AON), coenzyme A, and carbon dioxide [H]

COG id: COG0156

COG function: function code H; 7-keto-8-aminopelargonate synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridoxal-phosphate-dependent aminotransferase family. BioF subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657118, Length=392, Percent_Identity=33.1632653061224, Blast_Score=191, Evalue=9e-49,
Organism=Homo sapiens, GI83977440, Length=349, Percent_Identity=36.676217765043, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI83977442, Length=349, Percent_Identity=36.676217765043, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI83977444, Length=349, Percent_Identity=36.676217765043, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI284448556, Length=363, Percent_Identity=33.3333333333333, Blast_Score=185, Evalue=5e-47,
Organism=Homo sapiens, GI4502025, Length=358, Percent_Identity=33.7988826815642, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI40316939, Length=358, Percent_Identity=33.7988826815642, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI4758668, Length=354, Percent_Identity=30.225988700565, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI119220554, Length=356, Percent_Identity=28.3707865168539, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI5454084, Length=271, Percent_Identity=27.3062730627306, Blast_Score=109, Evalue=4e-24,
Organism=Escherichia coli, GI1786993, Length=346, Percent_Identity=46.8208092485549, Blast_Score=281, Evalue=4e-77,
Organism=Escherichia coli, GI1790046, Length=372, Percent_Identity=35.752688172043, Blast_Score=201, Evalue=7e-53,
Organism=Caenorhabditis elegans, GI71994529, Length=394, Percent_Identity=32.994923857868, Blast_Score=188, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI32566772, Length=353, Percent_Identity=27.1954674220963, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17560914, Length=365, Percent_Identity=26.8493150684932, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560912, Length=364, Percent_Identity=26.9230769230769, Blast_Score=117, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71982617, Length=286, Percent_Identity=26.2237762237762, Blast_Score=104, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI71982625, Length=286, Percent_Identity=26.2237762237762, Blast_Score=104, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6320438, Length=408, Percent_Identity=30.6372549019608, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6320267, Length=358, Percent_Identity=28.2122905027933, Blast_Score=126, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6323954, Length=301, Percent_Identity=26.578073089701, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24662918, Length=377, Percent_Identity=33.42175066313, Blast_Score=201, Evalue=9e-52,
Organism=Drosophila melanogaster, GI17137420, Length=364, Percent_Identity=32.6923076923077, Blast_Score=179, Evalue=2e-45,
Organism=Drosophila melanogaster, GI17136286, Length=357, Percent_Identity=26.3305322128852, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24653280, Length=338, Percent_Identity=25.4437869822485, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653276, Length=338, Percent_Identity=25.4437869822485, Blast_Score=108, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24653278, Length=338, Percent_Identity=25.4437869822485, Blast_Score=108, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022834
- InterPro:   IPR004839
- InterPro:   IPR004723
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00155 Aminotran_1_2 [H]

EC number: =2.3.1.47 [H]

Molecular weight: Translated: 40771; Mature: 40771

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLATLERGLADIDAQGLRRRRRIADTACSAHMSVDGREIVGFASNDYLGLAAHPRLIE
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHH
AFAEGARRYGAGSGGSHLLGGHSRAHAALEDELAAFSGGFSDAPRALYFSTGYMANLAAL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGRGATIFSDALNHASLIDGTRLSRANVQVYPHGDADALDALLRACDAPTKLIVSDT
HHHHCCCCHHHHHHHCHHHHCCCCEECCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECE
VFSMDGDVAPLARLVALAEAHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEEEECC
GKAAGAAGAFIVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAH
CCCCCCCCEEEEHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECEEEEEECCCCCHHHHH
LAALIERTRAILRATPWQPVDSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVP
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCCC
AGTSRLRISLSAAHSFADLARLEAALVTPIEAAA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MNLLATLERGLADIDAQGLRRRRRIADTACSAHMSVDGREIVGFASNDYLGLAAHPRLIE
CCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCEEEECCCHHHHH
AFAEGARRYGAGSGGSHLLGGHSRAHAALEDELAAFSGGFSDAPRALYFSTGYMANLAAL
HHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHH
TALAGRGATIFSDALNHASLIDGTRLSRANVQVYPHGDADALDALLRACDAPTKLIVSDT
HHHHCCCCHHHHHHHCHHHHCCCCEECCCEEEEEECCCHHHHHHHHHHCCCCCEEEEECE
VFSMDGDVAPLARLVALAEAHGAWLVVDDAHGFGVLGPQGRGALAAHGLRSPNLVYVGTL
EEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCEEEEECC
GKAAGAAGAFIVAHETVIEWLVQRARSYIFTTAAPPSVACAVSASLAVIASDEGDARRAH
CCCCCCCCEEEEHHHHHHHHHHHHHHHEEEECCCCCCEEEEEECEEEEEECCCCCHHHHH
LAALIERTRAILRATPWQPVDSHTAVQPLVIGSNEATLAAMAALDAQGLWVPAIRPPTVP
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCCC
AGTSRLRISLSAAHSFADLARLEAALVTPIEAAA
CCCCEEEEEEHHHHHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA