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The map label for this gene is thrS

Identifier: 83594816

GI number: 83594816

Start: 4012361

End: 4014292

Strand: Direct

Name: thrS

Synonym: Rru_A3487

Alternate gene names: 83594816

Gene position: 4012361-4014292 (Clockwise)

Preceding gene: 83594815

Following gene: 83594817

Centisome position: 92.18

GC content: 64.18

Gene sequence:

>1932_bases
ATGGTCGCCATTTCCCTGCCCGACGGCAGCGTTCGCAGCTATGAAGGCCCGGTGACGGGTCGCCAGATCGCCGAGGACAT
TGGTCCCGGCTTGGCCAAGGCAGCGCTGGCGGTCACTATCGACGGGACTATGAAGGATCTGAGCGTTCCCATCACTACGG
ACGCCAGCGTCAGCCTGATCACGGCGCGCGACGCCGCCGCCCTGGAGCTGCTGCGCCACGACGCCGCCCATGTGATGGCC
CAGGCGGTGCAAGAATTGTATCCCGGCACCCAGGTGACCATCGGTCCGGCGATCGAGAACGGCTTTTATTACGACTTCGC
CCGGGCCGAGCCGTTCACGCCCGAGGATCTGGAGCGCATCGAAGAGCGCATGCGCGAGATCGTCGGCCGCGATTTGCCGA
TCGTCCGCGAGGTCTGGGAGCGCGACGAGGCCATCGCCTATTTCACCAAGCTTGGTGAAACCTATAAGGCCGAGCTGATC
ACCGGCCTGCCGGCGAACGAGGATGTCAGCGTCTATCGCCAGGGCGACTGGCTTGACCTGTGCCGCGGCCCCCATCTGCC
GTCGACGGGCAAGCTCGGCACCGCCTTCAAGCTGATGAAGCTGGCCGGCGCCTATTGGCGGGGCGATTCGCGCAATCCGA
TGCTTCAGCGCATCTATGGCACCGCTTGGCCCGATCGCAAGCAGCTTGACGCCTATCTGACCATGCTGGCCGAGGCCGAA
AAGCGCGACCACCGCCGCGTCGGCCAGGAAATGGACCTGTTCCACCTTCAGGAAGAGGCCCAGGGCGCGGTCTTCTGGCA
TCCCAAGGGCTGGACGGTCTATCGCACCTTGCAAAACTATATGCGCCGTCGCCTGGATCTGGCCGGCTACGTCGAGGTCA
ACACCCCGATGCTGGTCGACCGCGCCCTTTGGGAAAAATCCGGGCATTGGGAGAAATTCCGCCAGAACATGTTCATCAGC
GAGGCCGAGGACAAGCTGCTGGCGATGAAGCCGATGAACTGCCCCTGCCATATCCAGATCTTCAAGCAGGGCATCAAAAG
CTATCGCGACCTGCCGCTGCGCATGGCCGAATTCGGCGCCTGTCACCGCAACGAGGCCTCGGGCGCCCTGCATGGCCTGA
TGCGGGTGCGCGCCTTCGTTCAAGATGACGCCCATATCTTCTGCACCGAAGATCAGATCGTTTCGGAAACCCAGGCCTTC
TGCGATCTTCTGAAAAGCGTCTACCGGGACCTGGGCTTCACCGAGATCAAGGTGAAGTTCTCCGACCGCCCCGAGGTTCG
CGCCGGCAGCGACGAGACCTGGGACAAGGCCGAAGCCGCCCTGCGCGAAGCCACCGACGCCGCCGGCCTGGAGTGGACCC
TCAACCCGGGCGAGGGCGCCTTCTATGGTCCCAAGCTGGAATTCGTCCTGCGCGACGCCATCGGCCGCGACTGGCAGTGC
GGCACCTTGCAGGTCGATTTCGTGCTTCCCGAACGCCTGGGCGCCGATTATGTCGGCGAGGATGGCGACAAGCATCGCCC
GGTCATGCTCCACCGCGCCATCCTTGGCACCTTCGAGCGCTTCATGGGCATCCTGATCGAGAACTGCGCCGGCCGTTTCC
CCTTCTGGCTGGCGCCGGTGCAGGTCGTGGTCGCGACCATCACCTCGGATGCCGACGATTACGCCCGCACGGTGCTCGCC
GCCCTCAAGGCCAAGGGCTTGCGCGCGGTCGCCGATCTGCGCAATGAAAAAATCAACTACAAGGTGCGCGAGCATTCGCT
GGCCAAGGTGCCGGTCATGCTGGTGGTCGGACGCAAGGAGGCCGAAACCGGCGGCGTGGCCTTGCGTCGCCTGGGCGGTC
AGAGCCAGGAAGTCCTTGCGCTTGATGAAGCCATTGCTACACTTGCCAACGAGTCGGTTGCTCCCGATCTGCGCGCCGCC
CCCCAGGGGTAA

Upstream 100 bases:

>100_bases
TAGGGGGCGATTTTGACCGAAAATCCCTAACACTTTTCGTTATCCCGCTCTAGAGTGCCCAATTCGATTTTCCCTGTTTC
CGACAAAGGACCGTAAGACG

Downstream 100 bases:

>100_bases
GGCGCAAAGGCCGGAGACGGACTCGACCAGGGTGCCGCTGGACGGCCCGGGCCCCTTCGGGAGATCTCTCCCGGGGGCCG
GAGCCGCCGCGGTCATCCCT

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 643; Mature: 643

Protein sequence:

>643_residues
MVAISLPDGSVRSYEGPVTGRQIAEDIGPGLAKAALAVTIDGTMKDLSVPITTDASVSLITARDAAALELLRHDAAHVMA
QAVQELYPGTQVTIGPAIENGFYYDFARAEPFTPEDLERIEERMREIVGRDLPIVREVWERDEAIAYFTKLGETYKAELI
TGLPANEDVSVYRQGDWLDLCRGPHLPSTGKLGTAFKLMKLAGAYWRGDSRNPMLQRIYGTAWPDRKQLDAYLTMLAEAE
KRDHRRVGQEMDLFHLQEEAQGAVFWHPKGWTVYRTLQNYMRRRLDLAGYVEVNTPMLVDRALWEKSGHWEKFRQNMFIS
EAEDKLLAMKPMNCPCHIQIFKQGIKSYRDLPLRMAEFGACHRNEASGALHGLMRVRAFVQDDAHIFCTEDQIVSETQAF
CDLLKSVYRDLGFTEIKVKFSDRPEVRAGSDETWDKAEAALREATDAAGLEWTLNPGEGAFYGPKLEFVLRDAIGRDWQC
GTLQVDFVLPERLGADYVGEDGDKHRPVMLHRAILGTFERFMGILIENCAGRFPFWLAPVQVVVATITSDADDYARTVLA
ALKAKGLRAVADLRNEKINYKVREHSLAKVPVMLVVGRKEAETGGVALRRLGGQSQEVLALDEAIATLANESVAPDLRAA
PQG

Sequences:

>Translated_643_residues
MVAISLPDGSVRSYEGPVTGRQIAEDIGPGLAKAALAVTIDGTMKDLSVPITTDASVSLITARDAAALELLRHDAAHVMA
QAVQELYPGTQVTIGPAIENGFYYDFARAEPFTPEDLERIEERMREIVGRDLPIVREVWERDEAIAYFTKLGETYKAELI
TGLPANEDVSVYRQGDWLDLCRGPHLPSTGKLGTAFKLMKLAGAYWRGDSRNPMLQRIYGTAWPDRKQLDAYLTMLAEAE
KRDHRRVGQEMDLFHLQEEAQGAVFWHPKGWTVYRTLQNYMRRRLDLAGYVEVNTPMLVDRALWEKSGHWEKFRQNMFIS
EAEDKLLAMKPMNCPCHIQIFKQGIKSYRDLPLRMAEFGACHRNEASGALHGLMRVRAFVQDDAHIFCTEDQIVSETQAF
CDLLKSVYRDLGFTEIKVKFSDRPEVRAGSDETWDKAEAALREATDAAGLEWTLNPGEGAFYGPKLEFVLRDAIGRDWQC
GTLQVDFVLPERLGADYVGEDGDKHRPVMLHRAILGTFERFMGILIENCAGRFPFWLAPVQVVVATITSDADDYARTVLA
ALKAKGLRAVADLRNEKINYKVREHSLAKVPVMLVVGRKEAETGGVALRRLGGQSQEVLALDEAIATLANESVAPDLRAA
PQG
>Mature_643_residues
MVAISLPDGSVRSYEGPVTGRQIAEDIGPGLAKAALAVTIDGTMKDLSVPITTDASVSLITARDAAALELLRHDAAHVMA
QAVQELYPGTQVTIGPAIENGFYYDFARAEPFTPEDLERIEERMREIVGRDLPIVREVWERDEAIAYFTKLGETYKAELI
TGLPANEDVSVYRQGDWLDLCRGPHLPSTGKLGTAFKLMKLAGAYWRGDSRNPMLQRIYGTAWPDRKQLDAYLTMLAEAE
KRDHRRVGQEMDLFHLQEEAQGAVFWHPKGWTVYRTLQNYMRRRLDLAGYVEVNTPMLVDRALWEKSGHWEKFRQNMFIS
EAEDKLLAMKPMNCPCHIQIFKQGIKSYRDLPLRMAEFGACHRNEASGALHGLMRVRAFVQDDAHIFCTEDQIVSETQAF
CDLLKSVYRDLGFTEIKVKFSDRPEVRAGSDETWDKAEAALREATDAAGLEWTLNPGEGAFYGPKLEFVLRDAIGRDWQC
GTLQVDFVLPERLGADYVGEDGDKHRPVMLHRAILGTFERFMGILIENCAGRFPFWLAPVQVVVATITSDADDYARTVLA
ALKAKGLRAVADLRNEKINYKVREHSLAKVPVMLVVGRKEAETGGVALRRLGGQSQEVLALDEAIATLANESVAPDLRAA
PQG

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38202255, Length=631, Percent_Identity=39.6196513470681, Blast_Score=466, Evalue=1e-131,
Organism=Homo sapiens, GI38570111, Length=634, Percent_Identity=38.1703470031546, Blast_Score=462, Evalue=1e-130,
Organism=Homo sapiens, GI20070344, Length=631, Percent_Identity=37.8763866877972, Blast_Score=419, Evalue=1e-117,
Organism=Escherichia coli, GI1788013, Length=639, Percent_Identity=51.1737089201878, Blast_Score=692, Evalue=0.0,
Organism=Escherichia coli, GI1786392, Length=140, Percent_Identity=28.5714285714286, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI71984184, Length=642, Percent_Identity=40.3426791277259, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71984192, Length=642, Percent_Identity=40.3426791277259, Blast_Score=487, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322112, Length=659, Percent_Identity=38.6949924127466, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322654, Length=409, Percent_Identity=36.4303178484108, Blast_Score=280, Evalue=5e-76,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=39.3650793650794, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=39.3650793650794, Blast_Score=471, Evalue=1e-133,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=39.3650793650794, Blast_Score=471, Evalue=1e-133,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_RHORT (Q2RNL4)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428568.1
- ProteinModelPortal:   Q2RNL4
- SMR:   Q2RNL4
- STRING:   Q2RNL4
- GeneID:   3836940
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3487
- NMPDR:   fig|1085.1.peg.2010
- eggNOG:   COG0441
- HOGENOM:   HBG352811
- OMA:   FHPKGMV
- PhylomeDB:   Q2RNL4
- ProtClustDB:   PRK00413
- BioCyc:   RRUB269796:RRU_A3487-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 71930; Mature: 71930

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVAISLPDGSVRSYEGPVTGRQIAEDIGPGLAKAALAVTIDGTMKDLSVPITTDASVSLI
CEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEEEEECCCHHHCCCEEECCCCEEEE
TARDAAALELLRHDAAHVMAQAVQELYPGTQVTIGPAIENGFYYDFARAEPFTPEDLERI
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHH
EERMREIVGRDLPIVREVWERDEAIAYFTKLGETYKAELITGLPANEDVSVYRQGDWLDL
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHEECCCCCCCCEEEECCCHHHH
CRGPHLPSTGKLGTAFKLMKLAGAYWRGDSRNPMLQRIYGTAWPDRKQLDAYLTMLAEAE
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRRVGQEMDLFHLQEEAQGAVFWHPKGWTVYRTLQNYMRRRLDLAGYVEVNTPMLVD
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RALWEKSGHWEKFRQNMFISEAEDKLLAMKPMNCPCHIQIFKQGIKSYRDLPLRMAEFGA
HHHHHCCCCHHHHHHCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEASGALHGLMRVRAFVQDDAHIFCTEDQIVSETQAFCDLLKSVYRDLGFTEIKVKF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SDRPEVRAGSDETWDKAEAALREATDAAGLEWTLNPGEGAFYGPKLEFVLRDAIGRDWQC
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCC
GTLQVDFVLPERLGADYVGEDGDKHRPVMLHRAILGTFERFMGILIENCAGRFPFWLAPV
CEEEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QVVVATITSDADDYARTVLAALKAKGLRAVADLRNEKINYKVREHSLAKVPVMLVVGRKE
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCC
AETGGVALRRLGGQSQEVLALDEAIATLANESVAPDLRAAPQG
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MVAISLPDGSVRSYEGPVTGRQIAEDIGPGLAKAALAVTIDGTMKDLSVPITTDASVSLI
CEEEECCCCCCCCCCCCCCHHHHHHHHCCCHHHEEEEEEECCCHHHCCCEEECCCCEEEE
TARDAAALELLRHDAAHVMAQAVQELYPGTQVTIGPAIENGFYYDFARAEPFTPEDLERI
ECCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEECCCCCCCHHHHHHH
EERMREIVGRDLPIVREVWERDEAIAYFTKLGETYKAELITGLPANEDVSVYRQGDWLDL
HHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHCCHHHHHHEECCCCCCCCEEEECCCHHHH
CRGPHLPSTGKLGTAFKLMKLAGAYWRGDSRNPMLQRIYGTAWPDRKQLDAYLTMLAEAE
HCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHH
KRDHRRVGQEMDLFHLQEEAQGAVFWHPKGWTVYRTLQNYMRRRLDLAGYVEVNTPMLVD
HHHHHHHCCHHHHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHCCCCEEEEECCCHHHH
RALWEKSGHWEKFRQNMFISEAEDKLLAMKPMNCPCHIQIFKQGIKSYRDLPLRMAEFGA
HHHHHCCCCHHHHHHCCCHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEASGALHGLMRVRAFVQDDAHIFCTEDQIVSETQAFCDLLKSVYRDLGFTEIKVKF
CCCCCHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
SDRPEVRAGSDETWDKAEAALREATDAAGLEWTLNPGEGAFYGPKLEFVLRDAIGRDWQC
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCEECCHHHHHHHHHCCCCCCC
GTLQVDFVLPERLGADYVGEDGDKHRPVMLHRAILGTFERFMGILIENCAGRFPFWLAPV
CEEEEEEEEHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
QVVVATITSDADDYARTVLAALKAKGLRAVADLRNEKINYKVREHSLAKVPVMLVVGRKE
HHHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEECCCCCCCCCEEEEECCCC
AETGGVALRRLGGQSQEVLALDEAIATLANESVAPDLRAAPQG
CCCCHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA