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The map label for this gene is gcvA [H]

Identifier: 83594697

GI number: 83594697

Start: 3884763

End: 3885686

Strand: Reverse

Name: gcvA [H]

Synonym: Rru_A3368

Alternate gene names: 83594697

Gene position: 3885686-3884763 (Counterclockwise)

Preceding gene: 83594700

Following gene: 83594693

Centisome position: 89.27

GC content: 67.32

Gene sequence:

>924_bases
ATGCCCCGGGATCTGCCGCCGCTCAACGCCTTGCGCGCCTTCACCGCGGCGGCCAAACGGCTGTCGTTCACCCAGGCGGC
CGAGGATCTGGGGGTCACCCAGGCCGCCGTCAGCCATCAGATCCGCGGGTTGGAAGATTGGCTGGGCGTGGCGCTTTTCC
GCCGCCGCAACAAGCAGGTGGTGATGACCGAGGCCGGTCAGGTCTATGCCCTGGCCATCGGTCAGGCCCTTGATGAAATC
GCCGAGGCGACGGCGGCGCTGGTGCGCAATGATCCGCGTGCCGGCATGCTGACGATCTCGACCATGCCGAGCTTCGCCAT
CAAATGGCTGGTCCAGCGCCTGGGCGGCTTCATCAACGCCAATCCCGATATCGAGGTCCGGCTGCACACCACCCGCGAGT
TGGTCAATTTCGCCACCGAGGGCATCGATGTCGCCATCCGCATGACCCGCGAGGTCGGCGACGATCTCCATGCCGATCTG
TTGCTGGAAGAGGAACTGTTTCCGGTGTGCAGCCCGGCGCTGATGCGCGGCATCCGGCCGCTGCGCCAGCCCTCCGACCT
CAGCCAGCACCTGTTGCTCCAGGACGTGGGGATTTCCTGGGTCTCCTGGCTCGACCCCGCCGGAGCCCTCGACGTGCGGC
CGGCCCGGGGCCCGGGCTATCTCGATTCGGCCCTGGCCATCCAGGCCTGCATCGAAAGCCAGGGGGTGATGCTGGGGCGC
ACGGTGATGGTTGAAGACGACCTCAAGGCCGGGCGGCTGGTCGAACCCTTCACCCTGCGCGTGCCCAGCCCGATGAAATA
CTGGCTGGTCTGCCCCCCCGGCCACCTCGCCCGCCCCGGTGTGCGGGCCTTCCGCGAATGGATCCTGGCCGAAGCCAGCA
AATGGACCAAGGCCCGTTCCAGCGCCCACGGCGTGGAAGATTAA

Upstream 100 bases:

>100_bases
GACCATTTGTTGACCGGATACTGCGCTTTTCCCCTTGACTCGACAAACGAGTAATTCTCCCATAGTGCATGAGAAAAAGT
AATCTGAAGGAAGGTGGGCC

Downstream 100 bases:

>100_bases
AACTCCACCCCCGGCTGGGGGCCGATGCCGGCGCGGAAGGGGTGTTTGATCATCTTCATCTCGGTCACCAGATCGGCGGC
CTCGATCAGCGCCTCGGGAG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MPRDLPPLNALRAFTAAAKRLSFTQAAEDLGVTQAAVSHQIRGLEDWLGVALFRRRNKQVVMTEAGQVYALAIGQALDEI
AEATAALVRNDPRAGMLTISTMPSFAIKWLVQRLGGFINANPDIEVRLHTTRELVNFATEGIDVAIRMTREVGDDLHADL
LLEEELFPVCSPALMRGIRPLRQPSDLSQHLLLQDVGISWVSWLDPAGALDVRPARGPGYLDSALAIQACIESQGVMLGR
TVMVEDDLKAGRLVEPFTLRVPSPMKYWLVCPPGHLARPGVRAFREWILAEASKWTKARSSAHGVED

Sequences:

>Translated_307_residues
MPRDLPPLNALRAFTAAAKRLSFTQAAEDLGVTQAAVSHQIRGLEDWLGVALFRRRNKQVVMTEAGQVYALAIGQALDEI
AEATAALVRNDPRAGMLTISTMPSFAIKWLVQRLGGFINANPDIEVRLHTTRELVNFATEGIDVAIRMTREVGDDLHADL
LLEEELFPVCSPALMRGIRPLRQPSDLSQHLLLQDVGISWVSWLDPAGALDVRPARGPGYLDSALAIQACIESQGVMLGR
TVMVEDDLKAGRLVEPFTLRVPSPMKYWLVCPPGHLARPGVRAFREWILAEASKWTKARSSAHGVED
>Mature_306_residues
PRDLPPLNALRAFTAAAKRLSFTQAAEDLGVTQAAVSHQIRGLEDWLGVALFRRRNKQVVMTEAGQVYALAIGQALDEIA
EATAALVRNDPRAGMLTISTMPSFAIKWLVQRLGGFINANPDIEVRLHTTRELVNFATEGIDVAIRMTREVGDDLHADLL
LEEELFPVCSPALMRGIRPLRQPSDLSQHLLLQDVGISWVSWLDPAGALDVRPARGPGYLDSALAIQACIESQGVMLGRT
VMVEDDLKAGRLVEPFTLRVPSPMKYWLVCPPGHLARPGVRAFREWILAEASKWTKARSSAHGVED

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=294, Percent_Identity=44.8979591836735, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1786448, Length=290, Percent_Identity=32.7586206896552, Blast_Score=140, Evalue=1e-34,
Organism=Escherichia coli, GI1788706, Length=290, Percent_Identity=32.0689655172414, Blast_Score=134, Evalue=5e-33,
Organism=Escherichia coli, GI1786401, Length=292, Percent_Identity=27.0547945205479, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI87081978, Length=270, Percent_Identity=30, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=26.0135135135135, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787128, Length=288, Percent_Identity=27.7777777777778, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787589, Length=150, Percent_Identity=32.6666666666667, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI157672245, Length=157, Percent_Identity=33.1210191082803, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1788887, Length=143, Percent_Identity=35.6643356643357, Blast_Score=64, Evalue=9e-12,
Organism=Escherichia coli, GI1789440, Length=242, Percent_Identity=23.9669421487603, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33754; Mature: 33623

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRDLPPLNALRAFTAAAKRLSFTQAAEDLGVTQAAVSHQIRGLEDWLGVALFRRRNKQV
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
VMTEAGQVYALAIGQALDEIAEATAALVRNDPRAGMLTISTMPSFAIKWLVQRLGGFINA
EEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCC
NPDIEVRLHTTRELVNFATEGIDVAIRMTREVGDDLHADLLLEEELFPVCSPALMRGIRP
CCCEEEEEEHHHHHHHHHHCCHHEEEEEHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCHH
LRQPSDLSQHLLLQDVGISWVSWLDPAGALDVRPARGPGYLDSALAIQACIESQGVMLGR
CCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECE
TVMVEDDLKAGRLVEPFTLRVPSPMKYWLVCPPGHLARPGVRAFREWILAEASKWTKARS
EEEEECCCCCCCEECCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SAHGVED
CCCCCCC
>Mature Secondary Structure 
PRDLPPLNALRAFTAAAKRLSFTQAAEDLGVTQAAVSHQIRGLEDWLGVALFRRRNKQV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCHHHHHHHHHHHHCCCEE
VMTEAGQVYALAIGQALDEIAEATAALVRNDPRAGMLTISTMPSFAIKWLVQRLGGFINA
EEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCCC
NPDIEVRLHTTRELVNFATEGIDVAIRMTREVGDDLHADLLLEEELFPVCSPALMRGIRP
CCCEEEEEEHHHHHHHHHHCCHHEEEEEHHHHCCCHHHHHHHHHHHHHCCCHHHHHCCHH
LRQPSDLSQHLLLQDVGISWVSWLDPAGALDVRPARGPGYLDSALAIQACIESQGVMLGR
CCCCCHHHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECE
TVMVEDDLKAGRLVEPFTLRVPSPMKYWLVCPPGHLARPGVRAFREWILAEASKWTKARS
EEEEECCCCCCCEECCEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
SAHGVED
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]