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The map label for this gene is ada [H]

Identifier: 83594686

GI number: 83594686

Start: 3869691

End: 3871151

Strand: Direct

Name: ada [H]

Synonym: Rru_A3357

Alternate gene names: 83594686

Gene position: 3869691-3871151 (Clockwise)

Preceding gene: 83594682

Following gene: 83594687

Centisome position: 88.9

GC content: 74.47

Gene sequence:

>1461_bases
ATGACCCAACACGATCCGAACCCGCCCAGCGATCCGTCGCTTTGCCACCGCGCCGTCGCCGCCCGCGACAAGCGCTTCGA
CGGCCGCTTCTTCACCTGCGTGCGCACCACCGGCATCTTCTGCCGGCCGATCTGCCCGGCGCGCACCCCGGCCATGGAGA
ACTGCCTGTTCGTCGCCACCGCCGCCCAGGCCCTGGCCGCCGGCTTCCGCCCCTGCCTGCGCTGTCGCCCCGAAGCCGCG
CCCGGCCTTGGCGGCTGGCTGGGAACGGCGGCCAGCGTCAACCGCGCCCTGGCGCTGATCGGCGAGGGCGCCCTTGACGA
GGGCAGCGTCGCCGCCCTGGCCGAGCGCCTGGGGCTGGGCGAGCGCCATCTGCGCCGCCTGTTCCTTCATCACCTGGGGA
TCTCGCCCGGGGCGGTGGCCCAGGCGCAACGACTGCTGTTTGCCAAGACGCTGATCAGCGATACGGCGCTGCCGATGACC
GAGATCGCCCTGGCGGCGGGCTATGGCAGCCTGCGCCGCTTCAATCACGCCCTGCGCACCGCCTATGGCTGCCCACCCAC
CGCCCTGCGCCGGGCCAAAGGCGCCGCGATCGTCGGCGAGGGCGGGCCGGTGCTGCGTCTGGCCGCCCGCCAACCCTTCG
ATTGGCCGGGGCTTTTGGCCTTCTTCCGCCAGCGCGCCGTTCCCGGGTTGGAGCGCGTCGAGGGCGACACCTATGTCCGC
GCCATCGCCATCGGCGCGGCGCGCGGCGTCGTCACCATTCGCGGGTCGGCCGAGGGGCTGGTCGTCACCCCCTCCCTCGA
CCGGCCCGAGGGGCTGGCGGCGCTGGTCGCCCGCCTGCGCCGGGTGTTCGATCTCGATGCCGATATCGGCGCGATCGGCG
CCCATCTTGGGGCCGACCCGCTGCTGGCCCCCCTGGTCGCCGCCCGTCCCGGCCTGCGGGTGCCCGGGGCCTGGGACGGG
TTCGAACTGGCGGTGCGGGCGATCCTCGGCCAGCAGGTCAGCGTCGCCGCCGCCACCACCCTGGCCGGCCGGCTGGTCGG
CGCGTTTGGCGAGCCCTTGACCAACGCCCCGCCGGCCGGGCCGTCGCGGCTGTTCCCCACGGCGGCGCGGCTGGCCGAGG
CCGATTTGGGCGGCCTGGGGCTGACCACCGCCCGGGCCAAGGCGATCAGCGGGCTGGCCCGGGCGGTGGTCGAGACCCCG
GGCCTTCTTGATCCCGGTCCCGATCTTGACTCCGCCGTCGCCCGGCTTTGTCGGCTGCCCGGCATCGGTCCCTGGACCGC
CCAGTATATCGCCCTGCGCGCCCTGGGCGAGGCCGACGCCTTGCCGGTCGGCGATATCGGAGTGCTGCGCGCCCTGGCCG
AAGACGGGGTGCGCCCCACCCCGGCCGCCCTGCTGGCCCGCGCCGAAGACTGGCGTCCCTGGCGGTCCTATGCCGTTTTA
CACCTGTGGAGCATGCCCTGA

Upstream 100 bases:

>100_bases
CCTTCCATAATTTTCCAGGGACTTTCCCTTTAGGGAAACCCGCCGCGGTCACGTCCGAAAACGGCGAGACGGCGCCTTCC
GGGTGTTCCACACTGGCGCC

Downstream 100 bases:

>100_bases
TGACACCGCTGTTTATCGATCGCCCCGACAGCCCGCTCGGCCGCTTGCTGGTGGTCGCCGACGACCAGAGCCTGCGTGGC
GTGGATTTCGCCGATAACGA

Product: transcriptional regulator Ada / DNA-3-methyladenine glycosylase II / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MTQHDPNPPSDPSLCHRAVAARDKRFDGRFFTCVRTTGIFCRPICPARTPAMENCLFVATAAQALAAGFRPCLRCRPEAA
PGLGGWLGTAASVNRALALIGEGALDEGSVAALAERLGLGERHLRRLFLHHLGISPGAVAQAQRLLFAKTLISDTALPMT
EIALAAGYGSLRRFNHALRTAYGCPPTALRRAKGAAIVGEGGPVLRLAARQPFDWPGLLAFFRQRAVPGLERVEGDTYVR
AIAIGAARGVVTIRGSAEGLVVTPSLDRPEGLAALVARLRRVFDLDADIGAIGAHLGADPLLAPLVAARPGLRVPGAWDG
FELAVRAILGQQVSVAAATTLAGRLVGAFGEPLTNAPPAGPSRLFPTAARLAEADLGGLGLTTARAKAISGLARAVVETP
GLLDPGPDLDSAVARLCRLPGIGPWTAQYIALRALGEADALPVGDIGVLRALAEDGVRPTPAALLARAEDWRPWRSYAVL
HLWSMP

Sequences:

>Translated_486_residues
MTQHDPNPPSDPSLCHRAVAARDKRFDGRFFTCVRTTGIFCRPICPARTPAMENCLFVATAAQALAAGFRPCLRCRPEAA
PGLGGWLGTAASVNRALALIGEGALDEGSVAALAERLGLGERHLRRLFLHHLGISPGAVAQAQRLLFAKTLISDTALPMT
EIALAAGYGSLRRFNHALRTAYGCPPTALRRAKGAAIVGEGGPVLRLAARQPFDWPGLLAFFRQRAVPGLERVEGDTYVR
AIAIGAARGVVTIRGSAEGLVVTPSLDRPEGLAALVARLRRVFDLDADIGAIGAHLGADPLLAPLVAARPGLRVPGAWDG
FELAVRAILGQQVSVAAATTLAGRLVGAFGEPLTNAPPAGPSRLFPTAARLAEADLGGLGLTTARAKAISGLARAVVETP
GLLDPGPDLDSAVARLCRLPGIGPWTAQYIALRALGEADALPVGDIGVLRALAEDGVRPTPAALLARAEDWRPWRSYAVL
HLWSMP
>Mature_485_residues
TQHDPNPPSDPSLCHRAVAARDKRFDGRFFTCVRTTGIFCRPICPARTPAMENCLFVATAAQALAAGFRPCLRCRPEAAP
GLGGWLGTAASVNRALALIGEGALDEGSVAALAERLGLGERHLRRLFLHHLGISPGAVAQAQRLLFAKTLISDTALPMTE
IALAAGYGSLRRFNHALRTAYGCPPTALRRAKGAAIVGEGGPVLRLAARQPFDWPGLLAFFRQRAVPGLERVEGDTYVRA
IAIGAARGVVTIRGSAEGLVVTPSLDRPEGLAALVARLRRVFDLDADIGAIGAHLGADPLLAPLVAARPGLRVPGAWDGF
ELAVRAILGQQVSVAAATTLAGRLVGAFGEPLTNAPPAGPSRLFPTAARLAEADLGGLGLTTARAKAISGLARAVVETPG
LLDPGPDLDSAVARLCRLPGIGPWTAQYIALRALGEADALPVGDIGVLRALAEDGVRPTPAALLARAEDWRPWRSYAVLH
LWSMP

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG0122

COG function: function code L; 3-methyladenine DNA glycosylase/8-oxoguanine DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=283, Percent_Identity=40.2826855123675, Blast_Score=156, Evalue=3e-39,
Organism=Escherichia coli, GI1788542, Length=128, Percent_Identity=44.53125, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 50841; Mature: 50710

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQHDPNPPSDPSLCHRAVAARDKRFDGRFFTCVRTTGIFCRPICPARTPAMENCLFVAT
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHH
AAQALAAGFRPCLRCRPEAAPGLGGWLGTAASVNRALALIGEGALDEGSVAALAERLGLG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
ERHLRRLFLHHLGISPGAVAQAQRLLFAKTLISDTALPMTEIALAAGYGSLRRFNHALRT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHH
AYGCPPTALRRAKGAAIVGEGGPVLRLAARQPFDWPGLLAFFRQRAVPGLERVEGDTYVR
HCCCCHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCEEE
AIAIGAARGVVTIRGSAEGLVVTPSLDRPEGLAALVARLRRVFDLDADIGAIGAHLGADP
HEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCH
LLAPLVAARPGLRVPGAWDGFELAVRAILGQQVSVAAATTLAGRLVGAFGEPLTNAPPAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PSRLFPTAARLAEADLGGLGLTTARAKAISGLARAVVETPGLLDPGPDLDSAVARLCRLP
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
GIGPWTAQYIALRALGEADALPVGDIGVLRALAEDGVRPTPAALLARAEDWRPWRSYAVL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHEEEE
HLWSMP
EEECCC
>Mature Secondary Structure 
TQHDPNPPSDPSLCHRAVAARDKRFDGRFFTCVRTTGIFCRPICPARTPAMENCLFVAT
CCCCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCEEEECCCCCCCCHHHHHHHHHH
AAQALAAGFRPCLRCRPEAAPGLGGWLGTAASVNRALALIGEGALDEGSVAALAERLGLG
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCC
ERHLRRLFLHHLGISPGAVAQAQRLLFAKTLISDTALPMTEIALAAGYGSLRRFNHALRT
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCHHHHHHHHHHHHH
AYGCPPTALRRAKGAAIVGEGGPVLRLAARQPFDWPGLLAFFRQRAVPGLERVEGDTYVR
HCCCCHHHHHHCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHCCCCHHHHCCCCEEE
AIAIGAARGVVTIRGSAEGLVVTPSLDRPEGLAALVARLRRVFDLDADIGAIGAHLGADP
HEEEECCCCEEEEECCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCH
LLAPLVAARPGLRVPGAWDGFELAVRAILGQQVSVAAATTLAGRLVGAFGEPLTNAPPAG
HHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PSRLFPTAARLAEADLGGLGLTTARAKAISGLARAVVETPGLLDPGPDLDSAVARLCRLP
CCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCC
GIGPWTAQYIALRALGEADALPVGDIGVLRALAEDGVRPTPAALLARAEDWRPWRSYAVL
CCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCCCCHHHHEEEE
HLWSMP
EEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]