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The map label for this gene is ogt [H]

Identifier: 83594687

GI number: 83594687

Start: 3871151

End: 3871759

Strand: Direct

Name: ogt [H]

Synonym: Rru_A3358

Alternate gene names: 83594687

Gene position: 3871151-3871759 (Clockwise)

Preceding gene: 83594686

Following gene: 83594694

Centisome position: 88.93

GC content: 68.31

Gene sequence:

>609_bases
ATGACACCGCTGTTTATCGATCGCCCCGACAGCCCGCTCGGCCGCTTGCTGGTGGTCGCCGACGACCAGAGCCTGCGTGG
CGTGGATTTCGCCGATAACGAAGCCCGTTTGATGCGTTTGCTCGAGGTTGGCGACGGGGCGGCGCTTATCCCCAAGGACG
ATCCTTTGGGGGCGTCCTCGGCTTTGGCGGCCTATTTCGCCGGCGATTTAACCGCCCTTGACGCTTTGCCCGTTCATTAC
GGCGGCACCCCCTTCCAGAACCGGGTGTGGAAGGCCCTGCGCACCATCGCCTTTGGCCAAACGCTGAGCTATGCCGCCCT
GGCCGAGCGCATCGGCGCGCCCGGGGCCTGCCGGGCGGTCGGGCGGGCCAATGGCCTCAATCCGCTGTCGCTGGTTTTAC
CCTGCCACCGGGTAATCGGCGGCTCGGGCGCCCTGACCGGCTATGCCGGCGGCCTTGCCCGCAAGGCCTGGCTGCTCCGC
CACGAGGGGCTGGCGATCGAGGGGTTCAGGCTGGCCCCGGCGCCGCGCTTATCAGATGTAGAAAACTGCCGGCCACCGAT
CCCCGGCGCTGGCCGACCGACCCCAGCGCCGTTCCGCCGGCATCCGTAA

Upstream 100 bases:

>100_bases
AAGACGGGGTGCGCCCCACCCCGGCCGCCCTGCTGGCCCGCGCCGAAGACTGGCGTCCCTGGCGGTCCTATGCCGTTTTA
CACCTGTGGAGCATGCCCTG

Downstream 100 bases:

>100_bases
CGGCGAACAACCGCCCCGCCTCGTCCCCCGCCTCGGCGATGATCGAGGCGTAGTGGAATTCATGGGCGGAAAACCCCTCT
CCCACCGCCCCCGGCCAGCC

Product: methylated-DNA--(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 202; Mature: 201

Protein sequence:

>202_residues
MTPLFIDRPDSPLGRLLVVADDQSLRGVDFADNEARLMRLLEVGDGAALIPKDDPLGASSALAAYFAGDLTALDALPVHY
GGTPFQNRVWKALRTIAFGQTLSYAALAERIGAPGACRAVGRANGLNPLSLVLPCHRVIGGSGALTGYAGGLARKAWLLR
HEGLAIEGFRLAPAPRLSDVENCRPPIPGAGRPTPAPFRRHP

Sequences:

>Translated_202_residues
MTPLFIDRPDSPLGRLLVVADDQSLRGVDFADNEARLMRLLEVGDGAALIPKDDPLGASSALAAYFAGDLTALDALPVHY
GGTPFQNRVWKALRTIAFGQTLSYAALAERIGAPGACRAVGRANGLNPLSLVLPCHRVIGGSGALTGYAGGLARKAWLLR
HEGLAIEGFRLAPAPRLSDVENCRPPIPGAGRPTPAPFRRHP
>Mature_201_residues
TPLFIDRPDSPLGRLLVVADDQSLRGVDFADNEARLMRLLEVGDGAALIPKDDPLGASSALAAYFAGDLTALDALPVHYG
GTPFQNRVWKALRTIAFGQTLSYAALAERIGAPGACRAVGRANGLNPLSLVLPCHRVIGGSGALTGYAGGLARKAWLLRH
EGLAIEGFRLAPAPRLSDVENCRPPIPGAGRPTPAPFRRHP

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=162, Percent_Identity=39.5061728395062, Blast_Score=97, Evalue=7e-21,
Organism=Escherichia coli, GI1787596, Length=167, Percent_Identity=51.497005988024, Blast_Score=171, Evalue=2e-44,
Organism=Escherichia coli, GI1788542, Length=160, Percent_Identity=38.125, Blast_Score=107, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI115533068, Length=104, Percent_Identity=43.2692307692308, Blast_Score=82, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=104, Percent_Identity=43.2692307692308, Blast_Score=82, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=89, Percent_Identity=43.8202247191011, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17137554, Length=97, Percent_Identity=43.298969072165, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 21303; Mature: 21172

Theoretical pI: Translated: 9.27; Mature: 9.27

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPLFIDRPDSPLGRLLVVADDQSLRGVDFADNEARLMRLLEVGDGAALIPKDDPLGASS
CCCCEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCHHH
ALAAYFAGDLTALDALPVHYGGTPFQNRVWKALRTIAFGQTLSYAALAERIGAPGACRAV
HHHHHHHCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHH
GRANGLNPLSLVLPCHRVIGGSGALTGYAGGLARKAWLLRHEGLAIEGFRLAPAPRLSDV
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECEEECCCCCCCCH
ENCRPPIPGAGRPTPAPFRRHP
HHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPLFIDRPDSPLGRLLVVADDQSLRGVDFADNEARLMRLLEVGDGAALIPKDDPLGASS
CCCEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCHHH
ALAAYFAGDLTALDALPVHYGGTPFQNRVWKALRTIAFGQTLSYAALAERIGAPGACRAV
HHHHHHHCCHHHHHCEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHH
GRANGLNPLSLVLPCHRVIGGSGALTGYAGGLARKAWLLRHEGLAIEGFRLAPAPRLSDV
HCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCEEECEEECCCCCCCCH
ENCRPPIPGAGRPTPAPFRRHP
HHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2825131; 9097039; 9278503; 1520330 [H]