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The map label for this gene is truA

Identifier: 83594681

GI number: 83594681

Start: 3865771

End: 3866607

Strand: Direct

Name: truA

Synonym: Rru_A3352

Alternate gene names: 83594681

Gene position: 3865771-3866607 (Clockwise)

Preceding gene: 83594680

Following gene: 83594682

Centisome position: 88.81

GC content: 67.86

Gene sequence:

>837_bases
ATGGTTCGCTATCGCTTGACCGTCGAATACGACGGGCGCGGCTATTGCGGCTGGCAGCGCCAGGACAACGGCCCAACCAT
TCAGCAATCCCTGGAAGAGGCGGCCTTCCGCCTGTGCGGGGTGGCCACCCGGGTTCACGGCTCGGGCCGCACCGATTCGG
GCGTCCATGCCCTGGGGCAGGTCGCCCATCTCGATCTGCCCAGGGCCTATGGCGCGGCGACGGTGATGAAGGCGCTCAAC
GCCCATCTGCGGCCCCAGCCGATCGCCGTGATCGATGCCGCGGAAGTGGCCGAGGACTTCCATGCCCGCTTTTCGGCCGA
GGAACGCTCCTATCGCTATCGCATCCTCAACCGCATCGCCCCGCCGACCCTTGACCAGGGACGGGTGTGGTGGGTGGCGC
GGCCGATGGAGGCGGCGATCATGGACGAGGCCGCCCAGGTTCTGGTCGGACGCCACGACTTCAGCAGCTTCCGCGCCGCC
GAATGTCAGGCCGACAGTCCGGTCAAAACCCTGAGCGAATTGCGCGTCACCCGGGTTGGCGACGAAATCCACGTCTTCGC
CCGCGCCCGCTCCTTCCTTCACCATCAAGTTCGCAATATGGTGGGAACCCTGGCCCTGGTCGGCGATGGCCGGTGGACGG
CCGATCGCCTGCGCGCCGCCCTCGAGGCTTGCGACCGCTCGGCCGCCGGCCCCACGGCGCCGCCCGATGGCCTTTATTTC
CTGCGCGTGCGCTTCCCCGGCGAAACGGCGGCCCCGGCGAAGGCTGGTCCTTTGGAAGCAGCCCCCCTGGGAGAAGCCCC
CCTGAAAGAAGCCACCCTGAAAGAAGACTGGCGATGA

Upstream 100 bases:

>100_bases
CCGCCTGCTGTGCCTGCAACGCCCGGGGCGCGGCCCTTTGGCCGCCGACGACTTCCTGCGGGGCTATGCCTTTCCCGCCG
GCCTATCGCTGACCTAGGCG

Downstream 100 bases:

>100_bases
CCTGGCTGACCCTGACCGGCGCGATCCTGTTCGAACTGGCCGGGACTACGGCGATGAAGCTCTCGGAGGGCTTCACCCGG
CTGCTGCCCTCGGTGGCGCT

Product: pseudouridylate synthase

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 278; Mature: 278

Protein sequence:

>278_residues
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQVAHLDLPRAYGAATVMKALN
AHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIAPPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAA
ECQADSPVKTLSELRVTRVGDEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR

Sequences:

>Translated_278_residues
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQVAHLDLPRAYGAATVMKALN
AHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIAPPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAA
ECQADSPVKTLSELRVTRVGDEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR
>Mature_278_residues
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQVAHLDLPRAYGAATVMKALN
AHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIAPPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAA
ECQADSPVKTLSELRVTRVGDEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=272, Percent_Identity=34.5588235294118, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI199559785, Length=282, Percent_Identity=29.4326241134752, Blast_Score=76, Evalue=4e-14,
Organism=Escherichia coli, GI1788657, Length=255, Percent_Identity=41.1764705882353, Blast_Score=174, Evalue=8e-45,
Organism=Drosophila melanogaster, GI116007752, Length=308, Percent_Identity=27.5974025974026, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_RHORT (Q2RNZ9)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428433.1
- ProteinModelPortal:   Q2RNZ9
- SMR:   Q2RNZ9
- STRING:   Q2RNZ9
- GeneID:   3836804
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3352
- NMPDR:   fig|1085.1.peg.2143
- eggNOG:   COG0101
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- PhylomeDB:   Q2RNZ9
- BioCyc:   RRUB269796:RRU_A3352-MONOMER
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 30619; Mature: 30619

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQ
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VAHLDLPRAYGAATVMKALNAHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIA
HHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAAECQADSPVKTLSELRVTRVG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
DEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
CHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MVRYRLTVEYDGRGYCGWQRQDNGPTIQQSLEEAAFRLCGVATRVHGSGRTDSGVHALGQ
CEEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
VAHLDLPRAYGAATVMKALNAHLRPQPIAVIDAAEVAEDFHARFSAEERSYRYRILNRIA
HHHCCCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHCC
PPTLDQGRVWWVARPMEAAIMDEAAQVLVGRHDFSSFRAAECQADSPVKTLSELRVTRVG
CCCCCCCCEEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHHHHHHHHHHHCC
DEIHVFARARSFLHHQVRNMVGTLALVGDGRWTADRLRAALEACDRSAAGPTAPPDGLYF
CHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEE
LRVRFPGETAAPAKAGPLEAAPLGEAPLKEATLKEDWR
EEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA