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The map label for this gene is fmt [H]

Identifier: 83594680

GI number: 83594680

Start: 3864838

End: 3865767

Strand: Direct

Name: fmt [H]

Synonym: Rru_A3351

Alternate gene names: 83594680

Gene position: 3864838-3865767 (Clockwise)

Preceding gene: 83594679

Following gene: 83594681

Centisome position: 88.79

GC content: 70.22

Gene sequence:

>930_bases
GTGACCCTTTCGGTGGTTTTCATGGGGACCCCGGCGTTTTCGGTCCCCATTCTTCACGCCTTGCATAACAACCCCGACCT
GACGCTGCGCGCCGTCTATTGCCAGCCGCCGCGCGCCGCCGGACGGGGCAAGAAGCCAAGGCCGACGCCGGTTCACGCCG
CAGCCGAGGCCTTGGGGATCCCCGTTTTCACCCCCGCCCGCTTGCGCGATGCCGCCGACCAGCAAGCCTTCGCCGAACTG
GCCGCCGATGTGGCGGTGGTCGCGGCCTATGGGCTGATCCTGCCCAAGGCGGTGCTCGACGCCCCGCGCCTGGGCTGCGT
CAATGTCCATGCCTCGCTGCTGCCGCGCTGGCGGGGCGCAGCACCCATCCACCGGGCGATCATGGCCGGCGACCGGGAAA
CCGGGGTGACCCTGATGCAGATGGACGAGGGGCTTGATACCGGGGCGATGCTGCGCATCGGCCGCGTCGCCATCACCGAG
CAGACCACCACCGCCAGCCTGCACGACACCTTGTCCGCCCTGGGGGCCGAGATGATCGGCCCGGCCCTGCGTGATCTGGC
GGCCGGAACCTTGAGCGGACAAGCCCAGCCGACCGAGGGCGTCACCTATGCCGCCAAGATCGACAAGGCCGAGGCCCGGC
TGGAGTGGCGAACCTCCGCCGCCGTCCTTGATCGCCAGATCCGCGCCCTGTCGCCCTTTCCCGGCGCGTGGTTCGAGCGC
GACGGCGAACGGATCAAGGTTCTGATGAGCCGGGTGGAGGAGGGCGTCCCCGCCGCCCCCCCCGGACAACTGCTTGACAA
CCAGCTGACGGTAGCCTGCGGCGAAGGGGCGGTCCGCCTGCTGTGCCTGCAACGCCCGGGGCGCGGCCCTTTGGCCGCCG
ACGACTTCCTGCGGGGCTATGCCTTTCCCGCCGGCCTATCGCTGACCTAG

Upstream 100 bases:

>100_bases
ACCATCTCGATGGTATTTTGTTCGTCGATTACCTGTCGTCGCTCAAACGCAATATGATGCTGCGCAAATTGCGGAAAATG
AAGCGGACGGGAGCCGAGGC

Downstream 100 bases:

>100_bases
GCGATGGTTCGCTATCGCTTGACCGTCGAATACGACGGGCGCGGCTATTGCGGCTGGCAGCGCCAGGACAACGGCCCAAC
CATTCAGCAATCCCTGGAAG

Product: methionyl-tRNA formyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 309; Mature: 308

Protein sequence:

>309_residues
MTLSVVFMGTPAFSVPILHALHNNPDLTLRAVYCQPPRAAGRGKKPRPTPVHAAAEALGIPVFTPARLRDAADQQAFAEL
AADVAVVAAYGLILPKAVLDAPRLGCVNVHASLLPRWRGAAPIHRAIMAGDRETGVTLMQMDEGLDTGAMLRIGRVAITE
QTTTASLHDTLSALGAEMIGPALRDLAAGTLSGQAQPTEGVTYAAKIDKAEARLEWRTSAAVLDRQIRALSPFPGAWFER
DGERIKVLMSRVEEGVPAAPPGQLLDNQLTVACGEGAVRLLCLQRPGRGPLAADDFLRGYAFPAGLSLT

Sequences:

>Translated_309_residues
MTLSVVFMGTPAFSVPILHALHNNPDLTLRAVYCQPPRAAGRGKKPRPTPVHAAAEALGIPVFTPARLRDAADQQAFAEL
AADVAVVAAYGLILPKAVLDAPRLGCVNVHASLLPRWRGAAPIHRAIMAGDRETGVTLMQMDEGLDTGAMLRIGRVAITE
QTTTASLHDTLSALGAEMIGPALRDLAAGTLSGQAQPTEGVTYAAKIDKAEARLEWRTSAAVLDRQIRALSPFPGAWFER
DGERIKVLMSRVEEGVPAAPPGQLLDNQLTVACGEGAVRLLCLQRPGRGPLAADDFLRGYAFPAGLSLT
>Mature_308_residues
TLSVVFMGTPAFSVPILHALHNNPDLTLRAVYCQPPRAAGRGKKPRPTPVHAAAEALGIPVFTPARLRDAADQQAFAELA
ADVAVVAAYGLILPKAVLDAPRLGCVNVHASLLPRWRGAAPIHRAIMAGDRETGVTLMQMDEGLDTGAMLRIGRVAITEQ
TTTASLHDTLSALGAEMIGPALRDLAAGTLSGQAQPTEGVTYAAKIDKAEARLEWRTSAAVLDRQIRALSPFPGAWFERD
GERIKVLMSRVEEGVPAAPPGQLLDNQLTVACGEGAVRLLCLQRPGRGPLAADDFLRGYAFPAGLSLT

Specific function: Modifies the free amino group of the aminoacyl moiety of methionyl-tRNA(fMet). The formyl group appears to play a dual role in the initiator identity of N-formylmethionyl-tRNA by:(I) promoting its recognition by IF2 and (II) impairing its binding to EFTu-

COG id: COG0223

COG function: function code J; Methionyl-tRNA formyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fmt family [H]

Homologues:

Organism=Homo sapiens, GI164663775, Length=319, Percent_Identity=31.0344827586207, Blast_Score=116, Evalue=2e-26,
Organism=Homo sapiens, GI238814322, Length=249, Percent_Identity=30.1204819277108, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI21614513, Length=215, Percent_Identity=31.1627906976744, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI1789683, Length=304, Percent_Identity=49.3421052631579, Blast_Score=273, Evalue=1e-74,
Organism=Escherichia coli, GI1788589, Length=228, Percent_Identity=30.7017543859649, Blast_Score=108, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI133930964, Length=287, Percent_Identity=25.4355400696864, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319458, Length=277, Percent_Identity=31.4079422382672, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI45550868, Length=304, Percent_Identity=30.2631578947368, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI28571984, Length=226, Percent_Identity=32.7433628318584, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI24585660, Length=250, Percent_Identity=26, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005794
- InterPro:   IPR005793
- InterPro:   IPR002376
- InterPro:   IPR011034
- InterPro:   IPR001555
- InterPro:   IPR015518 [H]

Pfam domain/function: PF02911 Formyl_trans_C; PF00551 Formyl_trans_N [H]

EC number: =2.1.2.9 [H]

Molecular weight: Translated: 32682; Mature: 32551

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLSVVFMGTPAFSVPILHALHNNPDLTLRAVYCQPPRAAGRGKKPRPTPVHAAAEALGI
CEEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
PVFTPARLRDAADQQAFAELAADVAVVAAYGLILPKAVLDAPRLGCVNVHASLLPRWRGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCC
APIHRAIMAGDRETGVTLMQMDEGLDTGAMLRIGRVAITEQTTTASLHDTLSALGAEMIG
CHHHHHHHCCCCCCCEEEEEECCCCCCCHHEEECEEEEECCCCHHHHHHHHHHHHHHHHH
PALRDLAAGTLSGQAQPTEGVTYAAKIDKAEARLEWRTSAAVLDRQIRALSPFPGAWFER
HHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
DGERIKVLMSRVEEGVPAAPPGQLLDNQLTVACGEGAVRLLCLQRPGRGPLAADDFLRGY
CCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
AFPAGLSLT
CCCCCCCCC
>Mature Secondary Structure 
TLSVVFMGTPAFSVPILHALHNNPDLTLRAVYCQPPRAAGRGKKPRPTPVHAAAEALGI
EEEEEEECCCCHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHCC
PVFTPARLRDAADQQAFAELAADVAVVAAYGLILPKAVLDAPRLGCVNVHASLLPRWRGA
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHCCCCCCC
APIHRAIMAGDRETGVTLMQMDEGLDTGAMLRIGRVAITEQTTTASLHDTLSALGAEMIG
CHHHHHHHCCCCCCCEEEEEECCCCCCCHHEEECEEEEECCCCHHHHHHHHHHHHHHHHH
PALRDLAAGTLSGQAQPTEGVTYAAKIDKAEARLEWRTSAAVLDRQIRALSPFPGAWFER
HHHHHHHHHCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHC
DGERIKVLMSRVEEGVPAAPPGQLLDNQLTVACGEGAVRLLCLQRPGRGPLAADDFLRGY
CCHHHHHHHHHHHHCCCCCCCHHHHCCCEEEEECCCCEEEEEEECCCCCCCCHHHHHHHH
AFPAGLSLT
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA