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The map label for this gene is def [H]

Identifier: 83594679

GI number: 83594679

Start: 3864323

End: 3864841

Strand: Direct

Name: def [H]

Synonym: Rru_A3350

Alternate gene names: 83594679

Gene position: 3864323-3864841 (Clockwise)

Preceding gene: 83594678

Following gene: 83594680

Centisome position: 88.78

GC content: 60.89

Gene sequence:

>519_bases
ATGGCGTTGCTTCCCATCCTGACGGCGCCTGATCCGCGCCTGACCAAAAAGGCTCTGCCGGTGGAAACGGTGGATGTCTC
CATCCGCAAGCTGATGGACGACATGCTTGAAACCATGTACCTCGCCCCCGGCATCGGCCTTGCCGCCCCCCAGGTCGGGG
TACTCAAGCGGGTGATCGTTCTCGATCCGGCGCGCGAGGACGAGGCGCCGCGCCCCATGCGTCTGGCCAATCCCGAGATC
ATCTGGAGCTCCGAGGATACCAAGCCCTATGAAGAGGGCTGCCTGTCGGTGCCCGAACAATACGATACGGTGGTGCGCCC
CGACCGCGTGCGGGTGCGCTACCTCGATCCCGACAATGAAATCCGCGAGATCGACGCCGATGGTTTGCTGGCCGTCATCT
TGCAGCACGAGATCGACCATCTCGATGGTATTTTGTTCGTCGATTACCTGTCGTCGCTCAAACGCAATATGATGCTGCGC
AAATTGCGGAAAATGAAGCGGACGGGAGCCGAGGCGTGA

Upstream 100 bases:

>100_bases
CCGCGGCTTTGCCGACCATTCTTGACGGATGGGCGCGGCCGGCTTATTTCCGACTCAGTGACAAACTCCCTCCAACGGCA
AGACGGAAAGACCGGGCGCA

Downstream 100 bases:

>100_bases
CCCTTTCGGTGGTTTTCATGGGGACCCCGGCGTTTTCGGTCCCCATTCTTCACGCCTTGCATAACAACCCCGACCTGACG
CTGCGCGCCGTCTATTGCCA

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 172; Mature: 171

Protein sequence:

>172_residues
MALLPILTAPDPRLTKKALPVETVDVSIRKLMDDMLETMYLAPGIGLAAPQVGVLKRVIVLDPAREDEAPRPMRLANPEI
IWSSEDTKPYEEGCLSVPEQYDTVVRPDRVRVRYLDPDNEIREIDADGLLAVILQHEIDHLDGILFVDYLSSLKRNMMLR
KLRKMKRTGAEA

Sequences:

>Translated_172_residues
MALLPILTAPDPRLTKKALPVETVDVSIRKLMDDMLETMYLAPGIGLAAPQVGVLKRVIVLDPAREDEAPRPMRLANPEI
IWSSEDTKPYEEGCLSVPEQYDTVVRPDRVRVRYLDPDNEIREIDADGLLAVILQHEIDHLDGILFVDYLSSLKRNMMLR
KLRKMKRTGAEA
>Mature_171_residues
ALLPILTAPDPRLTKKALPVETVDVSIRKLMDDMLETMYLAPGIGLAAPQVGVLKRVIVLDPAREDEAPRPMRLANPEII
WSSEDTKPYEEGCLSVPEQYDTVVRPDRVRVRYLDPDNEIREIDADGLLAVILQHEIDHLDGILFVDYLSSLKRNMMLRK
LRKMKRTGAEA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=159, Percent_Identity=32.7044025157233, Blast_Score=72, Evalue=2e-13,
Organism=Escherichia coli, GI1789682, Length=172, Percent_Identity=48.8372093023256, Blast_Score=152, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24645728, Length=166, Percent_Identity=31.9277108433735, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19486; Mature: 19355

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILTAPDPRLTKKALPVETVDVSIRKLMDDMLETMYLAPGIGLAAPQVGVLKRVIV
CCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LDPAREDEAPRPMRLANPEIIWSSEDTKPYEEGCLSVPEQYDTVVRPDRVRVRYLDPDNE
CCCCCCCCCCCCEECCCCEEEECCCCCCHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCH
IREIDADGLLAVILQHEIDHLDGILFVDYLSSLKRNMMLRKLRKMKRTGAEA
HHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALLPILTAPDPRLTKKALPVETVDVSIRKLMDDMLETMYLAPGIGLAAPQVGVLKRVIV
CCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
LDPAREDEAPRPMRLANPEIIWSSEDTKPYEEGCLSVPEQYDTVVRPDRVRVRYLDPDNE
CCCCCCCCCCCCEECCCCEEEECCCCCCHHHHHHCCCCHHHHHCCCCCCEEEEEECCCCH
IREIDADGLLAVILQHEIDHLDGILFVDYLSSLKRNMMLRKLRKMKRTGAEA
HHHCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA