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The map label for this gene is wcaJ [H]

Identifier: 83594432

GI number: 83594432

Start: 3570541

End: 3571992

Strand: Direct

Name: wcaJ [H]

Synonym: Rru_A3102

Alternate gene names: 83594432

Gene position: 3570541-3571992 (Clockwise)

Preceding gene: 83594431

Following gene: 83594433

Centisome position: 82.03

GC content: 58.06

Gene sequence:

>1452_bases
ATGATAAAAATTCTCGGTCACCACATATCCACACTATCCATAACCCTAGCCCTGTATGAGACGCTCATATTCATGTGCGT
TCTCTATTCCTTTATTTTCATAATGAGCTTCTTTTTTAACATACCTGTTCCAGAGAAGCTTTCTATGGTCATCCTTTTGG
CTCTGACCGCCACTATAGCCGCCGGATCCCTTGGCCTATACAATCAAAGGCTCTTTGGAGACTTTAAAGAATTCATCTGG
CGCATTGCTTTAACATTACCTCTTATCCTTCTGGCTGTATTGTTTGTTTCATTCGCCTACGGCGAGATAACAGCCACGGA
TCAATCGCCGTTTTATCTGGCCGCCGCCTTTGGCATTCCCGCCGCCCTGCTCCTGGTCGTTCTCGGCCGGGGAATCTGCT
TCCGCATTCCGGGGATCAAGGCGTTTCGTCGGCGGATCCTGGTCTTGGGCACGGGCGAAATGGCCGATCGGCTGGCGGCG
CTGGAAACCTCGCAACCCTATCGTCATTACGAGATCATCGGCTTTGTCGCCCTGGGCGACGACCCCGCACCCCTTGGCGA
AAAATGGCGGGTCTTCGACCGCAATTGCATCGAAACCCGCCACGATCTTCTGAAGATCTGCGCCGACCACAACATCAACG
AGGTGATCTTCGCCGCCAGCGAACGCCGCCGCGCCAATTCCAGCACCTTCGGCCTGCCGGTCTGGGATCTTTTGGAATGC
CGGCTGATGGGCATTCATGTCGCCGAATACGCCTCCTTCTGGGAGCGCGAGACCGGACGCATCGATCTTGATCACCTGCG
GCCGGGTTGGCTGATCTTTTCGGAAGGCTTTCGCAACAGCAGCCGGCGGATGATCGCCAAGCGCTTCTTCGACGTGATCA
CCGCCTTGTTGCTGGTCATCCTCAGCCTGCCGGTGCTGGTGGCGGCGGCCATCGCCGTGAAGCTGAGCAGCCCGGGACCG
GTCTTCTACCGCCAGGAACGGGTCGGGGTGAACGGCAAGCCCTTCAACCTGATCAAGTTCCGCAGCATGCCGATCGACGC
CGAAAAGAACGGCCCGGTCTGGGGCACCGTCAAGGATAAGCGGGCGACCGGCGTCGGCTCCTTGCTGCGCCGCACGCGAA
TCGACGAGATCCCCCAGGTTTTCAACGTGCTGCGCGGCGACATGAGCTTCATCGGCCCCCGCCCCGAACGCCCCGTCTTC
GTCGAAGAGCTCAGCCGGGAACTCGACTTCTATGGCGAGCGTCACGCGGTGCGCCCGGGGATCAGCGGCTGGGCCCAGAT
CAACTATCCCTATGGCGCCTCGGTCGCCGACGCCAAACAGAAACTGTCTTACGACCTTTACTACATCAAGAACGGCTCGC
TGTTCCTCGACATCATCATCTTGTTCCAAACCTTCCGCGTCGTTCTATGGCCGGAGGGATCGAAGGAGAAAGCCGGGGCG
GTCGTCCGCTAA

Upstream 100 bases:

>100_bases
TCCTGATACCTCTCGAGAATCGAATCGATACCTTTCAGCCATTGCCATACGGCGAGCGCCCCCGCAAACGCGATATGGAA
GATTAGAGAAAGGTATATCC

Downstream 100 bases:

>100_bases
GGAAAGGGGCGCTTGATGGTGATGGTCGTCGGATATTGCCTGGCCTGCGCCGCGTTCCTGGTGCTGATCGTCCAGGCGGT
CAGCGGGCGCAGGCGGACCG

Product: sugar transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MIKILGHHISTLSITLALYETLIFMCVLYSFIFIMSFFFNIPVPEKLSMVILLALTATIAAGSLGLYNQRLFGDFKEFIW
RIALTLPLILLAVLFVSFAYGEITATDQSPFYLAAAFGIPAALLLVVLGRGICFRIPGIKAFRRRILVLGTGEMADRLAA
LETSQPYRHYEIIGFVALGDDPAPLGEKWRVFDRNCIETRHDLLKICADHNINEVIFAASERRRANSSTFGLPVWDLLEC
RLMGIHVAEYASFWERETGRIDLDHLRPGWLIFSEGFRNSSRRMIAKRFFDVITALLLVILSLPVLVAAAIAVKLSSPGP
VFYRQERVGVNGKPFNLIKFRSMPIDAEKNGPVWGTVKDKRATGVGSLLRRTRIDEIPQVFNVLRGDMSFIGPRPERPVF
VEELSRELDFYGERHAVRPGISGWAQINYPYGASVADAKQKLSYDLYYIKNGSLFLDIIILFQTFRVVLWPEGSKEKAGA
VVR

Sequences:

>Translated_483_residues
MIKILGHHISTLSITLALYETLIFMCVLYSFIFIMSFFFNIPVPEKLSMVILLALTATIAAGSLGLYNQRLFGDFKEFIW
RIALTLPLILLAVLFVSFAYGEITATDQSPFYLAAAFGIPAALLLVVLGRGICFRIPGIKAFRRRILVLGTGEMADRLAA
LETSQPYRHYEIIGFVALGDDPAPLGEKWRVFDRNCIETRHDLLKICADHNINEVIFAASERRRANSSTFGLPVWDLLEC
RLMGIHVAEYASFWERETGRIDLDHLRPGWLIFSEGFRNSSRRMIAKRFFDVITALLLVILSLPVLVAAAIAVKLSSPGP
VFYRQERVGVNGKPFNLIKFRSMPIDAEKNGPVWGTVKDKRATGVGSLLRRTRIDEIPQVFNVLRGDMSFIGPRPERPVF
VEELSRELDFYGERHAVRPGISGWAQINYPYGASVADAKQKLSYDLYYIKNGSLFLDIIILFQTFRVVLWPEGSKEKAGA
VVR
>Mature_483_residues
MIKILGHHISTLSITLALYETLIFMCVLYSFIFIMSFFFNIPVPEKLSMVILLALTATIAAGSLGLYNQRLFGDFKEFIW
RIALTLPLILLAVLFVSFAYGEITATDQSPFYLAAAFGIPAALLLVVLGRGICFRIPGIKAFRRRILVLGTGEMADRLAA
LETSQPYRHYEIIGFVALGDDPAPLGEKWRVFDRNCIETRHDLLKICADHNINEVIFAASERRRANSSTFGLPVWDLLEC
RLMGIHVAEYASFWERETGRIDLDHLRPGWLIFSEGFRNSSRRMIAKRFFDVITALLLVILSLPVLVAAAIAVKLSSPGP
VFYRQERVGVNGKPFNLIKFRSMPIDAEKNGPVWGTVKDKRATGVGSLLRRTRIDEIPQVFNVLRGDMSFIGPRPERPVF
VEELSRELDFYGERHAVRPGISGWAQINYPYGASVADAKQKLSYDLYYIKNGSLFLDIIILFQTFRVVLWPEGSKEKAGA
VVR

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG2148

COG function: function code M; Sugar transferases involved in lipopolysaccharide synthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial sugar transferase family [H]

Homologues:

Organism=Escherichia coli, GI1788360, Length=180, Percent_Identity=45, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003362
- InterPro:   IPR017475
- InterPro:   IPR017473 [H]

Pfam domain/function: PF02397 Bac_transf [H]

EC number: NA

Molecular weight: Translated: 54463; Mature: 54463

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKILGHHISTLSITLALYETLIFMCVLYSFIFIMSFFFNIPVPEKLSMVILLALTATIA
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGSLGLYNQRLFGDFKEFIWRIALTLPLILLAVLFVSFAYGEITATDQSPFYLAAAFGIP
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHHHHH
AALLLVVLGRGICFRIPGIKAFRRRILVLGTGEMADRLAALETSQPYRHYEIIGFVALGD
HHHHHHHHCCCCEEECCCHHHHHCEEEEEECCHHHHHHHHHCCCCCCCEEEEEEEEEECC
DPAPLGEKWRVFDRNCIETRHDLLKICADHNINEVIFAASERRRANSSTFGLPVWDLLEC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCCHHHHHHH
RLMGIHVAEYASFWERETGRIDLDHLRPGWLIFSEGFRNSSRRMIAKRFFDVITALLLVI
HHHHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LSLPVLVAAAIAVKLSSPGPVFYRQERVGVNGKPFNLIKFRSMPIDAEKNGPVWGTVKDK
HHHHHHHHHHHHHEECCCCCEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCC
RATGVGSLLRRTRIDEIPQVFNVLRGDMSFIGPRPERPVFVEELSRELDFYGERHAVRPG
HHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
ISGWAQINYPYGASVADAKQKLSYDLYYIKNGSLFLDIIILFQTFRVVLWPEGSKEKAGA
CCCCEEEECCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCC
VVR
CCC
>Mature Secondary Structure
MIKILGHHISTLSITLALYETLIFMCVLYSFIFIMSFFFNIPVPEKLSMVILLALTATIA
CEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
AGSLGLYNQRLFGDFKEFIWRIALTLPLILLAVLFVSFAYGEITATDQSPFYLAAAFGIP
HCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCEEEEEHHHHH
AALLLVVLGRGICFRIPGIKAFRRRILVLGTGEMADRLAALETSQPYRHYEIIGFVALGD
HHHHHHHHCCCCEEECCCHHHHHCEEEEEECCHHHHHHHHHCCCCCCCEEEEEEEEEECC
DPAPLGEKWRVFDRNCIETRHDLLKICADHNINEVIFAASERRRANSSTFGLPVWDLLEC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECHHHHCCCCCCCCCCHHHHHHH
RLMGIHVAEYASFWERETGRIDLDHLRPGWLIFSEGFRNSSRRMIAKRFFDVITALLLVI
HHHHHHHHHHHHHHHCCCCCEEHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LSLPVLVAAAIAVKLSSPGPVFYRQERVGVNGKPFNLIKFRSMPIDAEKNGPVWGTVKDK
HHHHHHHHHHHHHEECCCCCEEEEHHHCCCCCCCCEEEEEECCCCCCCCCCCEEEEECCC
RATGVGSLLRRTRIDEIPQVFNVLRGDMSFIGPRPERPVFVEELSRELDFYGERHAVRPG
HHCCHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
ISGWAQINYPYGASVADAKQKLSYDLYYIKNGSLFLDIIILFQTFRVVLWPEGSKEKAGA
CCCCEEEECCCCCCHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHEEEECCCCCCCCCCC
VVR
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8759852; 9097040; 9278503 [H]