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The map label for this gene is gcvH

Identifier: 83594380

GI number: 83594380

Start: 3513648

End: 3514028

Strand: Reverse

Name: gcvH

Synonym: Rru_A3050

Alternate gene names: 83594380

Gene position: 3514028-3513648 (Counterclockwise)

Preceding gene: 83594381

Following gene: 83594379

Centisome position: 80.73

GC content: 62.73

Gene sequence:

>381_bases
ATGAGCAAACTCTATTTCACCGAAGATCACGAATGGGTCAGCGTCGATGACGATGGCATCGGCACCATCGGCATCACCGA
TTACGCCCAGAAGCAGCTTGGCGACGTGGTTTTCGTCGAACTGCCCGAAATCGGCCGCGAGATCGAGCGCGGCGGCGACG
CCGCCGTCGTCGAATCGGTCAAGGCGGCCAGCGAGGTCTATTCGCCGGTCAGCGGCGAGGTGGTCGAAGCCAATGACAGC
CTGCCCGAGGCGCCGGGACAGGTGAACACCGACGCCCTGGGCGACGGCTGGTTCTTCAAGGTGCGGCTGAGCGAGCCGGC
CGAGCTTGACGAGCTGATGGATCAAGAAGCCTACGACGCCTTCGTCGGCGAGCTCGATTAA

Upstream 100 bases:

>100_bases
CCATCGCTATCACAAGGCATAGCGTCCGGCGCGACGCGTCCCACTTATTCCTTTTGTCATCGCTTTTGTCGTCAACCGTT
ACCAGGCGAGGGTTCTGGTC

Downstream 100 bases:

>100_bases
CCAAGCGGCGCCGCCACCGGCCCGTCCGGGCATTTTTTCGAGGCTGAAGGACTCCCATGCGTTACCTCCCCCATTCCGAG
GCCGACCGCGCCGCCATGCT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSKLYFTEDHEWVSVDDDGIGTIGITDYAQKQLGDVVFVELPEIGREIERGGDAAVVESVKAASEVYSPVSGEVVEANDS
LPEAPGQVNTDALGDGWFFKVRLSEPAELDELMDQEAYDAFVGELD

Sequences:

>Translated_126_residues
MSKLYFTEDHEWVSVDDDGIGTIGITDYAQKQLGDVVFVELPEIGREIERGGDAAVVESVKAASEVYSPVSGEVVEANDS
LPEAPGQVNTDALGDGWFFKVRLSEPAELDELMDQEAYDAFVGELD
>Mature_125_residues
SKLYFTEDHEWVSVDDDGIGTIGITDYAQKQLGDVVFVELPEIGREIERGGDAAVVESVKAASEVYSPVSGEVVEANDSL
PEAPGQVNTDALGDGWFFKVRLSEPAELDELMDQEAYDAFVGELD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=126, Percent_Identity=50.7936507936508, Blast_Score=140, Evalue=2e-34,
Organism=Homo sapiens, GI49574537, Length=126, Percent_Identity=50.7936507936508, Blast_Score=140, Evalue=4e-34,
Organism=Escherichia coli, GI1789271, Length=121, Percent_Identity=47.9338842975207, Blast_Score=129, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17551294, Length=119, Percent_Identity=47.8991596638655, Blast_Score=128, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=47.4137931034483, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=46.1538461538462, Blast_Score=111, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17865652, Length=118, Percent_Identity=48.3050847457627, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_RHORT (Q2RPV0)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428132.1
- ProteinModelPortal:   Q2RPV0
- SMR:   Q2RPV0
- STRING:   Q2RPV0
- GeneID:   3836496
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A3050
- NMPDR:   fig|1085.1.peg.691
- eggNOG:   COG0509
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- PhylomeDB:   Q2RPV0
- ProtClustDB:   PRK01202
- BioCyc:   RRUB269796:RRU_A3050-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13702; Mature: 13571

Theoretical pI: Translated: 3.60; Mature: 3.60

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKLYFTEDHEWVSVDDDGIGTIGITDYAQKQLGDVVFVELPEIGREIERGGDAAVVESV
CCCCEECCCCCEEEECCCCCEEECCCHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHHH
KAASEVYSPVSGEVVEANDSLPEAPGQVNTDALGDGWFFKVRLSEPAELDELMDQEAYDA
HHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
FVGELD
HHCCCC
>Mature Secondary Structure 
SKLYFTEDHEWVSVDDDGIGTIGITDYAQKQLGDVVFVELPEIGREIERGGDAAVVESV
CCCEECCCCCEEEECCCCCEEECCCHHHHHHHCCEEEEECCHHHHHHHCCCCHHHHHHH
KAASEVYSPVSGEVVEANDSLPEAPGQVNTDALGDGWFFKVRLSEPAELDELMDQEAYDA
HHHHHHCCCCCCCEEECCCCCCCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHH
FVGELD
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA