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The map label for this gene is glyS

Identifier: 83594287

GI number: 83594287

Start: 3403455

End: 3405620

Strand: Direct

Name: glyS

Synonym: Rru_A2955

Alternate gene names: 83594287

Gene position: 3403455-3405620 (Clockwise)

Preceding gene: 83594286

Following gene: 83594288

Centisome position: 78.19

GC content: 67.87

Gene sequence:

>2166_bases
ATGGCCGAGTTGCTTCTGGAACTGTTGTCGGAAGAAATCCCCGCCCGCATGCAGGTGCGCGCCATCGAGGATCTGACCAA
GCTGGTCGGCACCGGTCTGGCCGAGGCCGGGCTGAGCCATGGCGGGCTGCGCGGCTTCGTCACCCCGCGCCGCCTGACCC
TGGTCGTCGACGGCCTGCCGGTCGCCCAGCCCGATGTCCACGAGGAGCGCCGCGGCCCCAAGGCCGACGCCCCCGACAAG
GCGATCGCCGGGTTCCTCGGCTCGCTGGGACTGACCCGCGACCAGTTGGAAACCCGCGAAACGCCCAAGGGCCCCTTCCT
GTTCGCCGTCATCGAGCGCAAGGGCCGGCCCACCGCCGAGGTGCTGCCCGAGATCATCTATCGCGCCCTCACCGCCCTGT
CCTGGCCGAAGTCGATGCGCTGGGCGCGCGGCCAGTTCACCTGGGTCCGCCCGCTCCATTCCATCCTGGCCGTCTTCGAG
GGCGCCCCTTTGCGCGGCGCCATCGGCCTTGGCGTCTCGCTCGAGGGTCTGCCGGTCGGCTTCATCGCCGATCCGGCCGC
CGCCCCCGAAGGCACGCCGTTGCTGGCGTTCGGAGCGACGACGCGCGGCCACCGCTTCCTGGCGCCCGAGCCCTTCCCCG
TGCGCGATTTCGCCGATTACGCCGCCCGCCTGCGCCAGGCCCATGTGCTGATCGACCGCGAGGAGCGCAAGCGGGTGATC
GCCGAGCGCGCCGCCGCCCTGGCCGCCGGCGAGGGCCTGCGGGTCAAGGACGATCCGGCCCTGCTCGAGGAAATCACCGG
GCTGGTCGAATGGCCGGTGCCCTTGATGGGCCATATCGACAAGGCCTTCATGGATGTGCCGGCCGAGGTGCTGACCACCT
CGATGCGCAGCCACCAGAAGTATTTCTCGGTCGAAAACGCCGATGGCACCCTGAACGCCCGCTTCGTCGTCGTGTCCAAC
ATGGATGCCACCGACAGCTCGGCCCGCGTCGTCGCCGGCAACGAGCGCGTGCTGCGCGCCCGGCTGTCGGACGCCAAGTT
CTTCTGGGACCAAGACCGCAAGGCGCCGCTGGCCGGCAAGGTCGAAGCCCTGGGCGCGCGCGTCTTCCACGCCAAACTGG
GCAGCGATCTTGATAAGGTCACCCGGGTGCGCGCCGGCGCGGCTTGGCTGGCCGCCCGGGTTCCCGGGGCCGACGCTAGC
TTGGTCGATCGCGCCGCCTTGCTGGCCAAGGCCGATCTGACCACCGGCATGGTCGGCGAATTCCCCGAACTCCAGGGCCT
GATGGGCCGCTATTACGCCCTGCACGACGGCGAGCCGCCGGCGGTGGCCCAGGCCATCCGGGACCACTACTCGCCCGTCG
GCCCCGGCGACGCCTGCCCGACCGCCGCCACCAGCGTTTGCGTCGCCCTGGCCGACAAGATCGACACCCTGGCCGGCTTC
TGGCTGATCGATGAAAAGCCCACCGGATCGAAGGATCCCTTCGCGCTGCGTCGCGCCGCCCTCGGCGTGATTCGCCTGAT
CGTGGAAAATAGTCTGCGCCTGCCCTTGCGAGAGGTGCTCGGCTCGGCAATCTCCACATATGGAGACCGGATGCTCGCCC
AATGCGGGAAAGCTGACGGAACATCCTCCGATGTGATAGAACACGATTTGATCGCTTTTATCGCTGAGCGTTTGAAGGTT
CAGCAGCGCGAAAAAGGGGTCCGTCACGATCTGATCGAGGCGGTCTTCTCGCAGGGTGGGGAGGACGATTTGGTCCGCCT
GCTCGCGCGCGTTTCGGCCCTGGCCGATTTCCTGGCCTCGGAAGAAGGGACCAATCTGCTCAGCGCCTATCGGCGGGCGA
CGAACATCGTGCGGATCGAAGAGAAAAAAGACGGAAAGCCGGTTTCGGGCGATCCCGACAACGCTCTTTTCGAACAGGAC
GAGGAACGCGCCCTGTCGCTAGCCCTTGATACTTTGCTCGGCGAGGTTTCCCCCTCCCTCGCCGCCGAGGATCACGCGGC
CGCCATGCGGGCTCTCGCCCGCCTGCGCGGTCCCGTCGACGCCTTTTTCGATCGGGTGACGGTGAACGCCGATACGCCCG
CTCTTCGCAACAATCGCCTGCGCCTGCTCGCGCGCATCGGCACCGCCATGGCCACCCTGGCGGACTTCTCAAAAATCGAG
GGATGA

Upstream 100 bases:

>100_bases
ATATCGGCCGGGTGCGGGCCCTGGCCAAGGGCTGTTGCGAGGGCTGGCTGCGGGCGCGCGGCCACCTGCCTCCGCTGACC
GGAACGGAGGGCTGAGCATC

Downstream 100 bases:

>100_bases
TCATGTCCAAGTGGGTTTACAGCTTCGGCAACGGCAAGGCCGAGGGCCGCGCCGATATGAAGAACCTCCTGGGCGGCAAG
GGTGCCAACCTGGCGGAAAT

Product: glycine--tRNA ligase

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 721; Mature: 720

Protein sequence:

>721_residues
MAELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLPVAQPDVHEERRGPKADAPDK
AIAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAEVLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFE
GAPLRGAIGLGVSLEGLPVGFIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVI
AERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQKYFSVENADGTLNARFVVVSN
MDATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGKVEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADAS
LVDRAALLAKADLTTGMVGEFPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGF
WLIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADGTSSDVIEHDLIAFIAERLKV
QQREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLASEEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQD
EERALSLALDTLLGEVSPSLAAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIE
G

Sequences:

>Translated_721_residues
MAELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLPVAQPDVHEERRGPKADAPDK
AIAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAEVLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFE
GAPLRGAIGLGVSLEGLPVGFIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVI
AERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQKYFSVENADGTLNARFVVVSN
MDATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGKVEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADAS
LVDRAALLAKADLTTGMVGEFPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGF
WLIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADGTSSDVIEHDLIAFIAERLKV
QQREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLASEEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQD
EERALSLALDTLLGEVSPSLAAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIE
G
>Mature_720_residues
AELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLPVAQPDVHEERRGPKADAPDKA
IAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAEVLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFEG
APLRGAIGLGVSLEGLPVGFIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVIA
ERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQKYFSVENADGTLNARFVVVSNM
DATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGKVEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADASL
VDRAALLAKADLTTGMVGEFPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGFW
LIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADGTSSDVIEHDLIAFIAERLKVQ
QREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLASEEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQDE
ERALSLALDTLLGEVSPSLAAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIEG

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=725, Percent_Identity=35.5862068965517, Blast_Score=390, Evalue=1e-109,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_RHORT (Q2RQ43)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_428039.1
- ProteinModelPortal:   Q2RQ43
- STRING:   Q2RQ43
- GeneID:   3836400
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A2955
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- PhylomeDB:   Q2RQ43
- ProtClustDB:   PRK01233
- BioCyc:   RRUB269796:RRU_A2955-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- SMART:   SM00836
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 77876; Mature: 77745

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLP
CHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEECCCC
VAQPDVHEERRGPKADAPDKAIAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAE
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCCHHH
VLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFEGAPLRGAIGLGVSLEGLPVG
HHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEECCCCEE
FIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVI
EECCCCCCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
AERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQK
HHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
YFSVENADGTLNARFVVVSNMDATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGK
HEEECCCCCCEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCHHHHCCCCCCCCCCH
VEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADASLVDRAALLAKADLTTGMVGE
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
FPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGF
CHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCE
WLIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TSSDVIEHDLIAFIAERLKVQQREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLAS
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
EEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQDEERALSLALDTLLGEVSPSL
CCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCH
AAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIE
HHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCC
G
C
>Mature Secondary Structure 
AELLLELLSEEIPARMQVRAIEDLTKLVGTGLAEAGLSHGGLRGFVTPRRLTLVVDGLP
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCEEEEEECCCC
VAQPDVHEERRGPKADAPDKAIAGFLGSLGLTRDQLETRETPKGPFLFAVIERKGRPTAE
CCCCCCHHHHCCCCCCCCHHHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCCHHH
VLPEIIYRALTALSWPKSMRWARGQFTWVRPLHSILAVFEGAPLRGAIGLGVSLEGLPVG
HHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCEEECCEEECCCCEE
FIADPAAAPEGTPLLAFGATTRGHRFLAPEPFPVRDFADYAARLRQAHVLIDREERKRVI
EECCCCCCCCCCEEEEECCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHH
AERAAALAAGEGLRVKDDPALLEEITGLVEWPVPLMGHIDKAFMDVPAEVLTTSMRSHQK
HHHHHHHHCCCCCEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHHHHHHHHHHH
YFSVENADGTLNARFVVVSNMDATDSSARVVAGNERVLRARLSDAKFFWDQDRKAPLAGK
HEEECCCCCCEEEEEEEEECCCCCCCCCEEEECCHHHHHHHHHCCHHHHCCCCCCCCCCH
VEALGARVFHAKLGSDLDKVTRVRAGAAWLAARVPGADASLVDRAALLAKADLTTGMVGE
HHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCC
FPELQGLMGRYYALHDGEPPAVAQAIRDHYSPVGPGDACPTAATSVCVALADKIDTLAGF
CHHHHHHHHHHEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCE
WLIDEKPTGSKDPFALRRAALGVIRLIVENSLRLPLREVLGSAISTYGDRMLAQCGKADG
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
TSSDVIEHDLIAFIAERLKVQQREKGVRHDLIEAVFSQGGEDDLVRLLARVSALADFLAS
CCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
EEGTNLLSAYRRATNIVRIEEKKDGKPVSGDPDNALFEQDEERALSLALDTLLGEVSPSL
CCCHHHHHHHHHHHCEEEEECCCCCCCCCCCCCCCHHCCHHHHHHHHHHHHHHHHCCCCH
AAEDHAAAMRALARLRGPVDAFFDRVTVNADTPALRNNRLRLLARIGTAMATLADFSKIE
HHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCCCHHCCHHHHHHHHHHHHHHHHHHHHCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA