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The map label for this gene is puuB [H]

Identifier: 83594206

GI number: 83594206

Start: 3310175

End: 3311482

Strand: Direct

Name: puuB [H]

Synonym: Rru_A2874

Alternate gene names: 83594206

Gene position: 3310175-3311482 (Clockwise)

Preceding gene: 83594202

Following gene: 83594208

Centisome position: 76.05

GC content: 70.49

Gene sequence:

>1308_bases
TTGTCTGGTTCGTCGCCTACCCCCGCTTCCCCCTCTTCCACGCCTCCCTCGTTATGGGCGGCGACCGCCCCAAGCGCCCC
GCCAACCCGGACCCTGGCCGGTGAGACCCGCGCCGACCTGGCGGTGATCGGCGCCGGCGTCACCGGTCTGTCCACGGCGC
TTCACGCCGCCGAGGCCGGGCTGACGGTGACGGTGGTCGAGGCCGAGGAAGCCGGCTTCGGCGGATCGGGGCGCAACAAC
GGTCAGGTGATCCCGACCCTGGCCAAGCCCGATCCCAGCGATCTGGTGGCCAAATGGGGCGAGGCCGGTGAACGCCTTGC
CCGCATGGTCGGCGAATCGGCCGCCCTGGTTTTCGATCTGATCGACCGCCACGCCATCGCCTGCGAGGCGGTTCAGCACG
GCTGGCTGCAGCCGGCCCACCGCCCCTCGCGCCTGACGGTCAGCCAGAAGCGCCACCGGGAATGGACGGCGCGTGGCCTG
GACTGCCGTCTGGTCGATCGCGCCGAGACCGCGGCGCTGACCGGCAGCGACTATTGGGCCGGCGGCCTGCTCTGCCCGAC
CGGGGGGCACGTCACCCCCCTGGCCCTGACCCGGGGATTGGCGCGGGCCGCCCAGAAGGCCGGGGCCACCGTCTATACGG
CCTCGCCGGTCACCGGCATCGACCGCCGGGGCAGCGACTGGCGGGTGGCCACCCCGGGTGGCGCCGTCACCGCCCGCGCC
GTGGTCATCGCCACCAGCGCCTATGGTCAAGGGGTGTGGCCCACCCTGGAACGCTCCATCGTGCCGGTGCGCAATTTCCA
GATGAGCACCCAGCCCCTGCCGCCCGAGGTTTTGGCCAAGGTCATCCCCGCCGATGTCGCCATCTCCGATACCCATGGCG
ATCTTTATTTCTTTCGCAAGACCGCCGACGGCCGGCTGGTCAGCGGCTGCACCCTGACCTCGCGCACCAGCGATCCGACC
GAGGCCAAGGCCCGGGTGATCGCCCGCATCCTCGAGGTCTTCCCCCAGGCCGGCCGCCAGACCATCGATTACTGCTGGCA
TGGTCAGCTTGATTTCACCCCGGATTTCCATCCGCGCTTCTACAGCCTGGAACCGGGCATTTTCGCCGCCATCGGCTATA
ACGGCCGGGGTCTGGCGCTGGGCGTCGCCGTTGGTCGCGAACTGGCCCGGGCGGCGGCGGGAACGCCGTTTTCCCAGCTT
GCCCTGCCCGATGGCGGCAAACCCCGCCCCCTGCCCTTCCACGGCCTGATCACCGGCGTCGCCCCGCTGATGATGCATTG
GTGGCGCCATAAGGACGGCCGCGACTAA

Upstream 100 bases:

>100_bases
CGCCGCTGGACGGAAACGCAAGCCCTTGCTAGCGTGCCGGGCGCGATATCCGAGGCTGCCTTGTAGATAGAAACCCTCTT
ATACAACAAAGGGGCGTAAA

Downstream 100 bases:

>100_bases
GGCCGCGCCGCCTCGGCGGCGGCGTTTCCTGGTTTCGCAACGCCAGCCAAATCGTTCACTCCCCGCATTGCCGGGGAACC
TGAACGGATGGCTGGCGTTT

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSGSSPTPASPSSTPPSLWAATAPSAPPTRTLAGETRADLAVIGAGVTGLSTALHAAEAGLTVTVVEAEEAGFGGSGRNN
GQVIPTLAKPDPSDLVAKWGEAGERLARMVGESAALVFDLIDRHAIACEAVQHGWLQPAHRPSRLTVSQKRHREWTARGL
DCRLVDRAETAALTGSDYWAGGLLCPTGGHVTPLALTRGLARAAQKAGATVYTASPVTGIDRRGSDWRVATPGGAVTARA
VVIATSAYGQGVWPTLERSIVPVRNFQMSTQPLPPEVLAKVIPADVAISDTHGDLYFFRKTADGRLVSGCTLTSRTSDPT
EAKARVIARILEVFPQAGRQTIDYCWHGQLDFTPDFHPRFYSLEPGIFAAIGYNGRGLALGVAVGRELARAAAGTPFSQL
ALPDGGKPRPLPFHGLITGVAPLMMHWWRHKDGRD

Sequences:

>Translated_435_residues
MSGSSPTPASPSSTPPSLWAATAPSAPPTRTLAGETRADLAVIGAGVTGLSTALHAAEAGLTVTVVEAEEAGFGGSGRNN
GQVIPTLAKPDPSDLVAKWGEAGERLARMVGESAALVFDLIDRHAIACEAVQHGWLQPAHRPSRLTVSQKRHREWTARGL
DCRLVDRAETAALTGSDYWAGGLLCPTGGHVTPLALTRGLARAAQKAGATVYTASPVTGIDRRGSDWRVATPGGAVTARA
VVIATSAYGQGVWPTLERSIVPVRNFQMSTQPLPPEVLAKVIPADVAISDTHGDLYFFRKTADGRLVSGCTLTSRTSDPT
EAKARVIARILEVFPQAGRQTIDYCWHGQLDFTPDFHPRFYSLEPGIFAAIGYNGRGLALGVAVGRELARAAAGTPFSQL
ALPDGGKPRPLPFHGLITGVAPLMMHWWRHKDGRD
>Mature_434_residues
SGSSPTPASPSSTPPSLWAATAPSAPPTRTLAGETRADLAVIGAGVTGLSTALHAAEAGLTVTVVEAEEAGFGGSGRNNG
QVIPTLAKPDPSDLVAKWGEAGERLARMVGESAALVFDLIDRHAIACEAVQHGWLQPAHRPSRLTVSQKRHREWTARGLD
CRLVDRAETAALTGSDYWAGGLLCPTGGHVTPLALTRGLARAAQKAGATVYTASPVTGIDRRGSDWRVATPGGAVTARAV
VIATSAYGQGVWPTLERSIVPVRNFQMSTQPLPPEVLAKVIPADVAISDTHGDLYFFRKTADGRLVSGCTLTSRTSDPTE
AKARVIARILEVFPQAGRQTIDYCWHGQLDFTPDFHPRFYSLEPGIFAAIGYNGRGLALGVAVGRELARAAAGTPFSQLA
LPDGGKPRPLPFHGLITGVAPLMMHWWRHKDGRD

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=407, Percent_Identity=32.9238329238329, Blast_Score=187, Evalue=8e-49,
Organism=Escherichia coli, GI87082084, Length=270, Percent_Identity=25.1851851851852, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 45912; Mature: 45781

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSSPTPASPSSTPPSLWAATAPSAPPTRTLAGETRADLAVIGAGVTGLSTALHAAEAG
CCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
LTVTVVEAEEAGFGGSGRNNGQVIPTLAKPDPSDLVAKWGEAGERLARMVGESAALVFDL
CEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IDRHAIACEAVQHGWLQPAHRPSRLTVSQKRHREWTARGLDCRLVDRAETAALTGSDYWA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCC
GGLLCPTGGHVTPLALTRGLARAAQKAGATVYTASPVTGIDRRGSDWRVATPGGAVTARA
CCEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCEEEE
VVIATSAYGQGVWPTLERSIVPVRNFQMSTQPLPPEVLAKVIPADVAISDTHGDLYFFRK
EEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEE
TADGRLVSGCTLTSRTSDPTEAKARVIARILEVFPQAGRQTIDYCWHGQLDFTPDFHPRF
CCCCEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHEECCCCCCCCCCCCCE
YSLEPGIFAAIGYNGRGLALGVAVGRELARAAAGTPFSQLALPDGGKPRPLPFHGLITGV
EEECCCEEEEECCCCCCEEEHHHHHHHHHHHHCCCCHHHEECCCCCCCCCCCHHHHHHHH
APLMMHWWRHKDGRD
HHHHHHHHHCCCCCC
>Mature Secondary Structure 
SGSSPTPASPSSTPPSLWAATAPSAPPTRTLAGETRADLAVIGAGVTGLSTALHAAEAG
CCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCC
LTVTVVEAEEAGFGGSGRNNGQVIPTLAKPDPSDLVAKWGEAGERLARMVGESAALVFDL
CEEEEEEECCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
IDRHAIACEAVQHGWLQPAHRPSRLTVSQKRHREWTARGLDCRLVDRAETAALTGSDYWA
HHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCEEECCCCCCC
GGLLCPTGGHVTPLALTRGLARAAQKAGATVYTASPVTGIDRRGSDWRVATPGGAVTARA
CCEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCEEECCCCCCEEEE
VVIATSAYGQGVWPTLERSIVPVRNFQMSTQPLPPEVLAKVIPADVAISDTHGDLYFFRK
EEEEECCCCCCCCHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCEEEECCCCCEEEEEE
TADGRLVSGCTLTSRTSDPTEAKARVIARILEVFPQAGRQTIDYCWHGQLDFTPDFHPRF
CCCCEEEECEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCEEHHEECCCCCCCCCCCCCE
YSLEPGIFAAIGYNGRGLALGVAVGRELARAAAGTPFSQLALPDGGKPRPLPFHGLITGV
EEECCCEEEEECCCCCCEEEHHHHHHHHHHHHCCCCHHHEECCCCCCCCCCCHHHHHHHH
APLMMHWWRHKDGRD
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]