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The map label for this gene is hsdBR [H]

Identifier: 83593822

GI number: 83593822

Start: 2890986

End: 2892236

Strand: Reverse

Name: hsdBR [H]

Synonym: Rru_A2487

Alternate gene names: 83593822

Gene position: 2892236-2890986 (Counterclockwise)

Preceding gene: 83593823

Following gene: 83593815

Centisome position: 66.45

GC content: 69.14

Gene sequence:

>1251_bases
ATGGATCTGTTCTCGCGGCTCGAGGGCGCCAAGGAACCCAAGGAGACTAAGGAGACCAAGGAGGCCAAGGAACCCAAGGA
ACCCAAGGAACCCAAGGAACCCAAGGAACCCAAGGAACCCAAGGAGACCAAGGAGGCCAAGGAACCTCCGGCGTTCGCTC
CTGAAACCCCGGCCGCGCCGCTACCGCCCTATGCGTCGCGTGATCTGATCGCTGCGCGCCTGCCGCTGATCTTTCCCGCC
GGTACGCCCAACCGCACCTATTGCGTGCGCGATCTGGCCGCCAGCACCGTGTTCGCCGCGCTCTATATCGGCGCGGTCGA
GGGACGCGGGTGTTTCCTGGGGCCGGTCCATGTCTACCGGATGACCAATGAGCAGGCGCTGTTGGCGGACGAGGCCGATC
GCCTCGCCTATGGTCTGGCGGTGCTGCGCAAGGGCGGCGGGATCAGCGGCAGCCGCTGGTATGCCGATAACACCCGCGAG
CCGATCCGCGATGAAACCCTGCGCGACGGGCTGGTGGCGATCGGCGCGGTGATCCGCCGGGGCGATCTGCCGACCACCTC
GGGCCAGCCGCGCTATGCCCTGAAGGCTGCCTTCGCCGCCCTGTTCGATCCGGCGCTTGACGGCCCGGCCCTGGACGCCG
CCATCGCCGCCTTCCAGGCCGCGCACCTCAGCCGTAGCGCCCTGGCCCGCGTGGCGATCATGCGCGCTGGCGCCACCGCC
GGGGCCGAGGGGGTGCTGGTGCGCTTTCCCAGCGGCGAGACCCGCCAGCTGGCGCCCGGCCCCAGTTCGATCATCGCCCA
GGCGGTGGTCGAGGTTTTCGCCCCGCGCTTTCTTGCGGCGCCGGCGGTGCTGTGGCTCAGCGAAAGCGGCAACAAGGTGG
TGCTGCGCGACGACGCCCTGGCCCATGCCATCGGCCTGCGCATCGAAGCCGATAAAACCCTGCCCGACCTGATCCTGGCC
GACCTTGGCCCCAAGGATCCGCTGCTGGTTTTCGTCGAGGTCGTCGCCACCGATGGCGCGCTCAGCCCCCAGCGCCAGCG
GGCCATTCACGCCCTGACCGAAGCCGCCGGTTTCTCGCCCGCCCAGGTGCTGTTCCTGACCGCCTATCAGGACCGCCAGT
CGCCGGGCTTTCGCAAGACCGTCGGCCAATTGGGCTGGGGCGGCTTCGTTTGGTTCGCGTCCGAGCCCGAGCGGCTCTTG
CGCCTGCACGACAGCGCCGGCGATCCCCGCACCCTGGCCGAGCTGCTCTAA

Upstream 100 bases:

>100_bases
CCGGCGATCTGGGGCCGCTGACGGCCCTGGTGGCCGCCGGCGCCCCGCGCGATGACATCGAAGCCGAATGCGCCCGGCTC
TATGGGAAGGGGCTTTGACC

Downstream 100 bases:

>100_bases
TCGCTGACCAGCCCGGCCAGACCGATGAAGGCCGGATAGGGGTGGGTGACCAAGCGAATGGGGACGACATCAAGGTAATC
GCGGAAGCGGCCCTTTTCGT

Product: BsuBIPstI restriction endonuclease-like

Products: NA

Alternate protein names: R.BsuBI; Endonuclease BsuBI; Type II restriction enzyme BsuBI [H]

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MDLFSRLEGAKEPKETKETKEAKEPKEPKEPKEPKEPKEPKETKEAKEPPAFAPETPAAPLPPYASRDLIAARLPLIFPA
GTPNRTYCVRDLAASTVFAALYIGAVEGRGCFLGPVHVYRMTNEQALLADEADRLAYGLAVLRKGGGISGSRWYADNTRE
PIRDETLRDGLVAIGAVIRRGDLPTTSGQPRYALKAAFAALFDPALDGPALDAAIAAFQAAHLSRSALARVAIMRAGATA
GAEGVLVRFPSGETRQLAPGPSSIIAQAVVEVFAPRFLAAPAVLWLSESGNKVVLRDDALAHAIGLRIEADKTLPDLILA
DLGPKDPLLVFVEVVATDGALSPQRQRAIHALTEAAGFSPAQVLFLTAYQDRQSPGFRKTVGQLGWGGFVWFASEPERLL
RLHDSAGDPRTLAELL

Sequences:

>Translated_416_residues
MDLFSRLEGAKEPKETKETKEAKEPKEPKEPKEPKEPKEPKETKEAKEPPAFAPETPAAPLPPYASRDLIAARLPLIFPA
GTPNRTYCVRDLAASTVFAALYIGAVEGRGCFLGPVHVYRMTNEQALLADEADRLAYGLAVLRKGGGISGSRWYADNTRE
PIRDETLRDGLVAIGAVIRRGDLPTTSGQPRYALKAAFAALFDPALDGPALDAAIAAFQAAHLSRSALARVAIMRAGATA
GAEGVLVRFPSGETRQLAPGPSSIIAQAVVEVFAPRFLAAPAVLWLSESGNKVVLRDDALAHAIGLRIEADKTLPDLILA
DLGPKDPLLVFVEVVATDGALSPQRQRAIHALTEAAGFSPAQVLFLTAYQDRQSPGFRKTVGQLGWGGFVWFASEPERLL
RLHDSAGDPRTLAELL
>Mature_416_residues
MDLFSRLEGAKEPKETKETKEAKEPKEPKEPKEPKEPKEPKETKEAKEPPAFAPETPAAPLPPYASRDLIAARLPLIFPA
GTPNRTYCVRDLAASTVFAALYIGAVEGRGCFLGPVHVYRMTNEQALLADEADRLAYGLAVLRKGGGISGSRWYADNTRE
PIRDETLRDGLVAIGAVIRRGDLPTTSGQPRYALKAAFAALFDPALDGPALDAAIAAFQAAHLSRSALARVAIMRAGATA
GAEGVLVRFPSGETRQLAPGPSSIIAQAVVEVFAPRFLAAPAVLWLSESGNKVVLRDDALAHAIGLRIEADKTLPDLILA
DLGPKDPLLVFVEVVATDGALSPQRQRAIHALTEAAGFSPAQVLFLTAYQDRQSPGFRKTVGQLGWGGFVWFASEPERLL
RLHDSAGDPRTLAELL

Specific function: Recognizes the double-stranded sequence CTGCAG and cleaves after A-5 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009528 [H]

Pfam domain/function: PF06616 BsuBI_PstI_RE [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 44586; Mature: 44586

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLFSRLEGAKEPKETKETKEAKEPKEPKEPKEPKEPKEPKETKEAKEPPAFAPETPAAP
CCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
LPPYASRDLIAARLPLIFPAGTPNRTYCVRDLAASTVFAALYIGAVEGRGCFLGPVHVYR
CCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEE
MTNEQALLADEADRLAYGLAVLRKGGGISGSRWYADNTREPIRDETLRDGLVAIGAVIRR
ECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHC
GDLPTTSGQPRYALKAAFAALFDPALDGPALDAAIAAFQAAHLSRSALARVAIMRAGATA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAEGVLVRFPSGETRQLAPGPSSIIAQAVVEVFAPRFLAAPAVLWLSESGNKVVLRDDAL
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCHH
AHAIGLRIEADKTLPDLILADLGPKDPLLVFVEVVATDGALSPQRQRAIHALTEAAGFSP
HHHHCCEEECCCCCHHHHHHCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCC
AQVLFLTAYQDRQSPGFRKTVGQLGWGGFVWFASEPERLLRLHDSAGDPRTLAELL
CEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHC
>Mature Secondary Structure
MDLFSRLEGAKEPKETKETKEAKEPKEPKEPKEPKEPKEPKETKEAKEPPAFAPETPAAP
CCHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCC
LPPYASRDLIAARLPLIFPAGTPNRTYCVRDLAASTVFAALYIGAVEGRGCFLGPVHVYR
CCCCCCCCHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEE
MTNEQALLADEADRLAYGLAVLRKGGGISGSRWYADNTREPIRDETLRDGLVAIGAVIRR
ECCCCEEHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHC
GDLPTTSGQPRYALKAAFAALFDPALDGPALDAAIAAFQAAHLSRSALARVAIMRAGATA
CCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
GAEGVLVRFPSGETRQLAPGPSSIIAQAVVEVFAPRFLAAPAVLWLSESGNKVVLRDDAL
CCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCEEEEECCHH
AHAIGLRIEADKTLPDLILADLGPKDPLLVFVEVVATDGALSPQRQRAIHALTEAAGFSP
HHHHCCEEECCCCCHHHHHHCCCCCCCCEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCCC
AQVLFLTAYQDRQSPGFRKTVGQLGWGGFVWFASEPERLLRLHDSAGDPRTLAELL
CEEEEEEEECCCCCCCHHHHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1480472 [H]