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The map label for this gene is yiaO [H]

Identifier: 83593574

GI number: 83593574

Start: 2599547

End: 2600575

Strand: Reverse

Name: yiaO [H]

Synonym: Rru_A2239

Alternate gene names: 83593574

Gene position: 2600575-2599547 (Counterclockwise)

Preceding gene: 83593575

Following gene: 83593573

Centisome position: 59.74

GC content: 64.14

Gene sequence:

>1029_bases
ATGAGAAAGCTATTCACCCTGGTGGCGGCGGCGCTGGCCTTCGCCGGCCTGTCGTCGTCGCCCGACGCCTCGGCGGCCGA
GTACAAGGCCGAATACAAGCTGTCCACCGTTCTTGGCAAACCGTTCCCCTGGGGGATCGGTGGCGAACGCTGGGCCGAAC
TGGTCAAGGAGAAGACCGAGGGCCGGATCGTCATGAAGATGTATCCGGGATCGTCCTTGCTGAACGGCGATCAGACCAAG
GAATTCACCGCCCTGCGCCAGGGGGTGATCGATATGGCGGTGGGATCGACGATCAACTGGTCGCCCCAGGTCAAGGAACT
CAACCTCTTCTCCATGCCCTTCCTGCTGCCCGATCACGCCGGCCTTGACGCCCTGACCAAGGGCGGCGTCGGCCAGCGGT
TGTTCGATCTTTTGGCGACCAAGGACGTGGTGCCGCTGGCCTGGGGCGAGAACGGCTTTCGCGAGCTTTCCAATTCCCGC
CATGCCATTCGCACCCCGGCCGATATGAAGGGGCTGAAGATCCGCGTCGTCGGCTCGCCGCTTTTTCTCGATACCTTCAG
CGCGCTCGGCGCTAATCCCACCCAGATGAGCTGGGCCGATGCCCAGCCTTCGCTGTCGACGGGGGCGGTGGACGGCCAGG
AAAACCCGCTGTCGGTTTTCATGACCGCCAAACTGCCGACCTTGGGCCAGAAGCACCTGACCCTGTGGGGCTATGTCGCC
GATCCGCTGATCTTCGTCGTCAACAAGGAGGTTTGGGAGTCGTGGAGCGAGGAGGATCGCGCCGCCGTGCGCGCCGCCGC
CCTTCAAGCCGCGGGCGAAGAGGTGGCCCTGGCTCGCAAGGGCATCACCGCCGAGGATGACGCCCTGCTCAAGGACATCA
CCGCCCAAGGGGTGAGCATCACCGCACTTTCTGCGGCCGAAAAGGCCGCCTTCCGCGATGCGACCCGCGCCGTTTATGAC
AAATGGAGCAAGCAGATCGGCGCCGATCTGGTGAAAGAGGCCGAAACGGCCATCGCCGCCGGTCGTTGA

Upstream 100 bases:

>100_bases
GGGTCAAAAGATTTAAAAATTTCTCAATGACAGGGGTGGACGCAGCTTTGTACCATCCCGCCTTCGGAAAACAGACCAAA
AACCACCGAAGGGACAGTCC

Downstream 100 bases:

>100_bases
CCCTTCCGGGGGCGCCAGGCGCGCTAGGCGCCCCCCTGTCCTTGCCAGCAAGAAGACCGCCATGTCCTCCCCCTCGACCG
AATCCGCCGCCAAAGATGAG

Product: TRAP dicarboxylate transporter subunit DctP

Products: NA

Alternate protein names: 2,3-DKG-binding protein; Extracytoplasmic solute receptor protein yiaO [H]

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MRKLFTLVAAALAFAGLSSSPDASAAEYKAEYKLSTVLGKPFPWGIGGERWAELVKEKTEGRIVMKMYPGSSLLNGDQTK
EFTALRQGVIDMAVGSTINWSPQVKELNLFSMPFLLPDHAGLDALTKGGVGQRLFDLLATKDVVPLAWGENGFRELSNSR
HAIRTPADMKGLKIRVVGSPLFLDTFSALGANPTQMSWADAQPSLSTGAVDGQENPLSVFMTAKLPTLGQKHLTLWGYVA
DPLIFVVNKEVWESWSEEDRAAVRAAALQAAGEEVALARKGITAEDDALLKDITAQGVSITALSAAEKAAFRDATRAVYD
KWSKQIGADLVKEAETAIAAGR

Sequences:

>Translated_342_residues
MRKLFTLVAAALAFAGLSSSPDASAAEYKAEYKLSTVLGKPFPWGIGGERWAELVKEKTEGRIVMKMYPGSSLLNGDQTK
EFTALRQGVIDMAVGSTINWSPQVKELNLFSMPFLLPDHAGLDALTKGGVGQRLFDLLATKDVVPLAWGENGFRELSNSR
HAIRTPADMKGLKIRVVGSPLFLDTFSALGANPTQMSWADAQPSLSTGAVDGQENPLSVFMTAKLPTLGQKHLTLWGYVA
DPLIFVVNKEVWESWSEEDRAAVRAAALQAAGEEVALARKGITAEDDALLKDITAQGVSITALSAAEKAAFRDATRAVYD
KWSKQIGADLVKEAETAIAAGR
>Mature_342_residues
MRKLFTLVAAALAFAGLSSSPDASAAEYKAEYKLSTVLGKPFPWGIGGERWAELVKEKTEGRIVMKMYPGSSLLNGDQTK
EFTALRQGVIDMAVGSTINWSPQVKELNLFSMPFLLPDHAGLDALTKGGVGQRLFDLLATKDVVPLAWGENGFRELSNSR
HAIRTPADMKGLKIRVVGSPLFLDTFSALGANPTQMSWADAQPSLSTGAVDGQENPLSVFMTAKLPTLGQKHLTLWGYVA
DPLIFVVNKEVWESWSEEDRAAVRAAALQAAGEEVALARKGITAEDDALLKDITAQGVSITALSAAEKAAFRDATRAVYD
KWSKQIGADLVKEAETAIAAGR

Specific function: Part of the tripartite ATP-independent periplasmic (TRAP) transport system yiaMNO involved in the uptake of 2,3- diketo-L-gulonate. This protein specifically binds 2,3-diketo-L- gulonate. Is not able to bind either L-ascorbate or dehydroascorbate [H]

COG id: COG1638

COG function: function code G; TRAP-type C4-dicarboxylate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 7 family [H]

Homologues:

Organism=Escherichia coli, GI1790004, Length=328, Percent_Identity=32.0121951219512, Blast_Score=154, Evalue=7e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018389
- InterPro:   IPR004682 [H]

Pfam domain/function: PF03480 SBP_bac_7 [H]

EC number: NA

Molecular weight: Translated: 36767; Mature: 36767

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKLFTLVAAALAFAGLSSSPDASAAEYKAEYKLSTVLGKPFPWGIGGERWAELVKEKTE
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
GRIVMKMYPGSSLLNGDQTKEFTALRQGVIDMAVGSTINWSPQVKELNLFSMPFLLPDHA
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCC
GLDALTKGGVGQRLFDLLATKDVVPLAWGENGFRELSNSRHAIRTPADMKGLKIRVVGSP
CCHHHHCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEECCCCCCCCEEEEEECCC
LFLDTFSALGANPTQMSWADAQPSLSTGAVDGQENPLSVFMTAKLPTLGQKHLTLWGYVA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEHH
DPLIFVVNKEVWESWSEEDRAAVRAAALQAAGEEVALARKGITAEDDALLKDITAQGVSI
CCEEEEECHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCEEE
TALSAAEKAAFRDATRAVYDKWSKQIGADLVKEAETAIAAGR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRKLFTLVAAALAFAGLSSSPDASAAEYKAEYKLSTVLGKPFPWGIGGERWAELVKEKTE
CHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCC
GRIVMKMYPGSSLLNGDQTKEFTALRQGVIDMAVGSTINWSPQVKELNLFSMPFLLPDHA
CEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCC
GLDALTKGGVGQRLFDLLATKDVVPLAWGENGFRELSNSRHAIRTPADMKGLKIRVVGSP
CCHHHHCCCHHHHHHHHHHHCCCEEEECCCHHHHHHHCCCCEECCCCCCCCEEEEEECCC
LFLDTFSALGANPTQMSWADAQPSLSTGAVDGQENPLSVFMTAKLPTLGQKHLTLWGYVA
HHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEHH
DPLIFVVNKEVWESWSEEDRAAVRAAALQAAGEEVALARKGITAEDDALLKDITAQGVSI
CCEEEEECHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCCHHHHHHHHHCCCEEE
TALSAAEKAAFRDATRAVYDKWSKQIGADLVKEAETAIAAGR
EEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503 [H]