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The map label for this gene is mdeA [H]

Identifier: 83593410

GI number: 83593410

Start: 2401827

End: 2403665

Strand: Direct

Name: mdeA [H]

Synonym: Rru_A2075

Alternate gene names: 83593410

Gene position: 2401827-2403665 (Clockwise)

Preceding gene: 83593408

Following gene: 83593411

Centisome position: 55.18

GC content: 65.69

Gene sequence:

>1839_bases
ATGACCGACGCCCTTTCCTATCCCGATGCCCTGCTCCGCCGCATTCTTGGCGAAACCCGGACCATCGCCATGGTCGGGGC
CTCGGCCAATTGGAACCGTCCAAGCTTCTTCGTGATGAAGTATCTGCAATCAAGGGGATTTCGGGTGATCCCGGTCAACC
CCGGCCTCGCCGGCCAGCAGATCCTGGGCGAGACGGTCTATGCCCGGTTGGCCGATGTTCCCGCCCCCTTCGAGATGGTC
GACATCTTCCGCAATTCGGACGCCGCCGGGGCGATCCTTGACGAGGCCGTCGCCCTGCAAGCCGACAAGGGCATCCGCAC
CGTCTGGATGCAGTTGTCCGTGCGCAACGACGCCGCGGCCGAACGCGGCGCCGCCGCTGGGCTTGAGGTGATCATGGACC
GCTGCCCCAAGATCGAATTCGGCCGCCTGGGCGGCGAGTTATCCTGGCAAGGGGTGAATTCGGGGATCATTTCCAGCAAG
GTGCGCCGGGCGCCCGGCCGCGAAACCGCCCAAGACCGCCGCCAATCGCCGCCGCCAGGGCAAGATCCCGCCGACAAGCC
GGCGCCCTTCGGCTTTGAAACCCGCGCCGTTCACGCCGGCGCCGCCCCCGATCCAACCACCGGCGCCCGCGCCACGCCGA
TCTATCAAACCACCTCTTACGTGTTCGAAGACACCGATCAGGCGGCCGCGTTGTTCAACCTGCATACCTTCGGCTATCTC
TATTCGCGACTGACCAATCCGACGGTCAGCGTGTTGGAGGAACGCATCGCCAGCCTGGAAGGCGGACGGGCGGCGGTCTG
TTGCGCCTCGGGCCATGCGGCGCAGTTCCTGACTTTTTTCACCCTGCTTGAACCGGGCGATCATTTCGTCGCCTCGCGCG
CCCTCTATGGCGGATCGCTGACCCAGTTCGGCCAGTCGTTCCGCAAGCTGGGCTGGGAGTGCAGCTTCGTTGATCCCACC
GATATCGACGCCTTCCGCGCCGCCATTGGTCCGCGCACCAAGGCGATCTTCCTGGAACTGCTGGCCAATCCGGGGGGGGT
GATCGTCGATGTCGAACAGGTCGCCCGCGTCGCCCAGCAGGCGGGCATTCCGCTGATCGTCGACAACACGCTGGCCACGC
CCTATCTGTGCCGCCCCTTCGACTGGGGCGCCGATCTGGTGGTTCATTCGACCACCAAGTTCCTGTCGGGACACGGCAAT
GCGGTTGGCGGCGCGGTCGTCGAAAGCGGCCGTTTCAACTGGTCGGCCAGCGATAAATTCCCCGGGCTGTCGCAGCCCGA
ACCGGCCTATCACGGAATGACCTTCCACGAGACCTTTGGCGATTTCGCCTTCACCACCAAGGCCCGCGCCGTCGCCCTGC
GCGATTTCGGTCCGGCGATGGCGCCGCAAAACGCCTTTCTGACCCTCACCGGCATCGAGACCCTGCCGCTGCGCATGGAT
CGCCACGTGGAAAACGCCAAAAAAGTCGCGGCCTTCCTCGCCGCCCATCCCAAGGTGTCGTGGGTCAGCTATGCCGGACT
GCCCGACAGCCCCTTCCACGGGCTTGCCGGCAAATATCTGCCCAAGGGCCCCGGAGCGGTGTTCACCTTCGGGGTCGTCG
GCGGCTTCGAAGCCGGCAAGAAGGTGGTCGAGGGAGTGCGGCTGTTCAGCCATCTCGCCAATGTCGGCGATACCCGCTCG
CTGATCCTCCACCCCGCCAGCACCACCCATCGCCAGCTTTCCGACGACCAACGCCAAGCCGCCGGGGCCGGCGACGAGGT
GATCCGCCTGTCGGTGGGCATCGAGACCGCCGCCGATCTGATCGCCGACCTTGACCAGGCCCTCGCCCTGATCGCGTGA

Upstream 100 bases:

>100_bases
CGCGTCGTCGCCATCGCTATCCTTCCCCCTGGGGCCCTTAGAACAGTAAACTCCAGCGGGTTTGTCTACAATTCCCTTCG
CCGTTTGATAAGGATCGCCC

Downstream 100 bases:

>100_bases
CTCCTCAGCCATGAGGGGGAAGGGGAAAGTCTTCTCGTTCCCCTTCCCCCTCATGGTATACCAGAAAAGCACTCGTCTTT
GTTTTATCTTTTTAAATCAA

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 612; Mature: 611

Protein sequence:

>612_residues
MTDALSYPDALLRRILGETRTIAMVGASANWNRPSFFVMKYLQSRGFRVIPVNPGLAGQQILGETVYARLADVPAPFEMV
DIFRNSDAAGAILDEAVALQADKGIRTVWMQLSVRNDAAAERGAAAGLEVIMDRCPKIEFGRLGGELSWQGVNSGIISSK
VRRAPGRETAQDRRQSPPPGQDPADKPAPFGFETRAVHAGAAPDPTTGARATPIYQTTSYVFEDTDQAAALFNLHTFGYL
YSRLTNPTVSVLEERIASLEGGRAAVCCASGHAAQFLTFFTLLEPGDHFVASRALYGGSLTQFGQSFRKLGWECSFVDPT
DIDAFRAAIGPRTKAIFLELLANPGGVIVDVEQVARVAQQAGIPLIVDNTLATPYLCRPFDWGADLVVHSTTKFLSGHGN
AVGGAVVESGRFNWSASDKFPGLSQPEPAYHGMTFHETFGDFAFTTKARAVALRDFGPAMAPQNAFLTLTGIETLPLRMD
RHVENAKKVAAFLAAHPKVSWVSYAGLPDSPFHGLAGKYLPKGPGAVFTFGVVGGFEAGKKVVEGVRLFSHLANVGDTRS
LILHPASTTHRQLSDDQRQAAGAGDEVIRLSVGIETAADLIADLDQALALIA

Sequences:

>Translated_612_residues
MTDALSYPDALLRRILGETRTIAMVGASANWNRPSFFVMKYLQSRGFRVIPVNPGLAGQQILGETVYARLADVPAPFEMV
DIFRNSDAAGAILDEAVALQADKGIRTVWMQLSVRNDAAAERGAAAGLEVIMDRCPKIEFGRLGGELSWQGVNSGIISSK
VRRAPGRETAQDRRQSPPPGQDPADKPAPFGFETRAVHAGAAPDPTTGARATPIYQTTSYVFEDTDQAAALFNLHTFGYL
YSRLTNPTVSVLEERIASLEGGRAAVCCASGHAAQFLTFFTLLEPGDHFVASRALYGGSLTQFGQSFRKLGWECSFVDPT
DIDAFRAAIGPRTKAIFLELLANPGGVIVDVEQVARVAQQAGIPLIVDNTLATPYLCRPFDWGADLVVHSTTKFLSGHGN
AVGGAVVESGRFNWSASDKFPGLSQPEPAYHGMTFHETFGDFAFTTKARAVALRDFGPAMAPQNAFLTLTGIETLPLRMD
RHVENAKKVAAFLAAHPKVSWVSYAGLPDSPFHGLAGKYLPKGPGAVFTFGVVGGFEAGKKVVEGVRLFSHLANVGDTRS
LILHPASTTHRQLSDDQRQAAGAGDEVIRLSVGIETAADLIADLDQALALIA
>Mature_611_residues
TDALSYPDALLRRILGETRTIAMVGASANWNRPSFFVMKYLQSRGFRVIPVNPGLAGQQILGETVYARLADVPAPFEMVD
IFRNSDAAGAILDEAVALQADKGIRTVWMQLSVRNDAAAERGAAAGLEVIMDRCPKIEFGRLGGELSWQGVNSGIISSKV
RRAPGRETAQDRRQSPPPGQDPADKPAPFGFETRAVHAGAAPDPTTGARATPIYQTTSYVFEDTDQAAALFNLHTFGYLY
SRLTNPTVSVLEERIASLEGGRAAVCCASGHAAQFLTFFTLLEPGDHFVASRALYGGSLTQFGQSFRKLGWECSFVDPTD
IDAFRAAIGPRTKAIFLELLANPGGVIVDVEQVARVAQQAGIPLIVDNTLATPYLCRPFDWGADLVVHSTTKFLSGHGNA
VGGAVVESGRFNWSASDKFPGLSQPEPAYHGMTFHETFGDFAFTTKARAVALRDFGPAMAPQNAFLTLTGIETLPLRMDR
HVENAKKVAAFLAAHPKVSWVSYAGLPDSPFHGLAGKYLPKGPGAVFTFGVVGGFEAGKKVVEGVRLFSHLANVGDTRSL
ILHPASTTHRQLSDDQRQAAGAGDEVIRLSVGIETAADLIADLDQALALIA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=419, Percent_Identity=32.6968973747017, Blast_Score=189, Evalue=5e-48,
Organism=Homo sapiens, GI299473759, Length=419, Percent_Identity=29.8329355608592, Blast_Score=159, Evalue=1e-38,
Organism=Homo sapiens, GI25453487, Length=418, Percent_Identity=29.1866028708134, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI1790375, Length=413, Percent_Identity=31.9612590799031, Blast_Score=194, Evalue=2e-50,
Organism=Escherichia coli, GI87081810, Length=130, Percent_Identity=53.8461538461538, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1789383, Length=428, Percent_Identity=28.2710280373832, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71980708, Length=442, Percent_Identity=31.6742081447964, Blast_Score=199, Evalue=3e-51,
Organism=Caenorhabditis elegans, GI17560056, Length=423, Percent_Identity=33.3333333333333, Blast_Score=188, Evalue=8e-48,
Organism=Caenorhabditis elegans, GI17538055, Length=422, Percent_Identity=32.4644549763033, Blast_Score=181, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6323333, Length=437, Percent_Identity=45.0800915331808, Blast_Score=343, Evalue=4e-95,
Organism=Saccharomyces cerevisiae, GI6319307, Length=423, Percent_Identity=31.2056737588652, Blast_Score=174, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321254, Length=445, Percent_Identity=25.6179775280899, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6321170, Length=373, Percent_Identity=26.8096514745308, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321904, Length=433, Percent_Identity=26.0969976905312, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI19922554, Length=424, Percent_Identity=31.1320754716981, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 65542; Mature: 65411

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00868 CYS_MET_METAB_PP ; PS00196 COPPER_BLUE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDALSYPDALLRRILGETRTIAMVGASANWNRPSFFVMKYLQSRGFRVIPVNPGLAGQQ
CCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHH
ILGETVYARLADVPAPFEMVDIFRNSDAAGAILDEAVALQADKGIRTVWMQLSVRNDAAA
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHH
ERGAAAGLEVIMDRCPKIEFGRLGGELSWQGVNSGIISSKVRRAPGRETAQDRRQSPPPG
HHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHCCCCCCCHHHHCCCCCCC
QDPADKPAPFGFETRAVHAGAAPDPTTGARATPIYQTTSYVFEDTDQAAALFNLHTFGYL
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHH
YSRLTNPTVSVLEERIASLEGGRAAVCCASGHAAQFLTFFTLLEPGDHFVASRALYGGSL
HHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCH
TQFGQSFRKLGWECSFVDPTDIDAFRAAIGPRTKAIFLELLANPGGVIVDVEQVARVAQQ
HHHHHHHHHHCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
AGIPLIVDNTLATPYLCRPFDWGADLVVHSTTKFLSGHGNAVGGAVVESGRFNWSASDKF
CCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCCCCCCHHEECCCCCCCCCCCC
PGLSQPEPAYHGMTFHETFGDFAFTTKARAVALRDFGPAMAPQNAFLTLTGIETLPLRMD
CCCCCCCCCCCCCCHHHHHCCHHEECHHHEEEEHHCCCCCCCCCCEEEEECCCCCCHHHH
RHVENAKKVAAFLAAHPKVSWVSYAGLPDSPFHGLAGKYLPKGPGAVFTFGVVGGFEAGK
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHH
KVVEGVRLFSHLANVGDTRSLILHPASTTHRQLSDDQRQAAGAGDEVIRLSVGIETAADL
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECHHHHHHH
IADLDQALALIA
HHHHHHHHHHCC
>Mature Secondary Structure 
TDALSYPDALLRRILGETRTIAMVGASANWNRPSFFVMKYLQSRGFRVIPVNPGLAGQQ
CCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEECCCCCCHHH
ILGETVYARLADVPAPFEMVDIFRNSDAAGAILDEAVALQADKGIRTVWMQLSVRNDAAA
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHHHHHHHHHHCCCCCEEEEEEEEECCCHHH
ERGAAAGLEVIMDRCPKIEFGRLGGELSWQGVNSGIISSKVRRAPGRETAQDRRQSPPPG
HHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCCCHHHHHHHCCCCCCCHHHHCCCCCCC
QDPADKPAPFGFETRAVHAGAAPDPTTGARATPIYQTTSYVFEDTDQAAALFNLHTFGYL
CCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCCEECHHHHCCCCCHHHHHHHHHHHHHH
YSRLTNPTVSVLEERIASLEGGRAAVCCASGHAAQFLTFFTLLEPGDHFVASRALYGGSL
HHHHCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHCCCCH
TQFGQSFRKLGWECSFVDPTDIDAFRAAIGPRTKAIFLELLANPGGVIVDVEQVARVAQQ
HHHHHHHHHHCCEEECCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCEEEEHHHHHHHHHH
AGIPLIVDNTLATPYLCRPFDWGADLVVHSTTKFLSGHGNAVGGAVVESGRFNWSASDKF
CCCCEEEECCCCCCCEECCCCCCCCEEEECHHHHHCCCCCCCCCHHEECCCCCCCCCCCC
PGLSQPEPAYHGMTFHETFGDFAFTTKARAVALRDFGPAMAPQNAFLTLTGIETLPLRMD
CCCCCCCCCCCCCCHHHHHCCHHEECHHHEEEEHHCCCCCCCCCCEEEEECCCCCCHHHH
RHVENAKKVAAFLAAHPKVSWVSYAGLPDSPFHGLAGKYLPKGPGAVFTFGVVGGFEAGK
HHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHH
KVVEGVRLFSHLANVGDTRSLILHPASTTHRQLSDDQRQAAGAGDEVIRLSVGIETAADL
HHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHCCCCCCEEEEEECHHHHHHH
IADLDQALALIA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]