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The map label for this gene is eno

Identifier: 83593220

GI number: 83593220

Start: 2183612

End: 2184889

Strand: Reverse

Name: eno

Synonym: Rru_A1885

Alternate gene names: 83593220

Gene position: 2184889-2183612 (Counterclockwise)

Preceding gene: 83593221

Following gene: 83593217

Centisome position: 50.19

GC content: 64.55

Gene sequence:

>1278_bases
GTGGCCGAGATTATCGATATCCACGCCCGCGAGATTCTGGACTCCCGCGGCAATCCCACCGTCGAAGTCGATGTGCTGCT
CGATAGCGGCGCCTTCGGACGGGCTGCCGTTCCGTCGGGTGCCTCGACCGGCGCGCATGAGGCCGTCGAACTGCGCGATG
GCGACAAGACGCGTTACGCGGGCAAGGGGGTTCTCAAGGCCGTCGAGGCCGTCAACGGCGAGTTGTTCAGCGCCCTTTCG
GGTCTGGACGCCACCGACCAGTTGCTGATCGATCAGGCGATGATTGATCTCGATGGTACCCCCAACAAGGCCCGCCTGGG
CGCCAACGCCATCCTCGGCGTCTCGCTGGCCTGCGCCAAGGCCGCCGCCGAGGAAGCCGAGCTGCCGCTCTATCGCTATA
TCGGCGGCGCGCGCTCGCACATCCTGCCGGTGCCGATGATGAACATCATCAACGGTGGCCAGCACGCCGATAACCCCATC
GACGTCCAGGAATTCATGATCATGCCCGTTTCGGCGCCGACGGTGGCCGACGCGGTGCGCATGGGCGCCGAAGTCTTCCA
TGCCTTGAAGAAGAAGCTGAAGGACGCCGGCCACAACACCAATGTCGGCGACGAGGGCGGCTTCGCCCCCAATCTCGCCA
GCGCCGACGAGGCCCTGGCCTTCATCGTCAAGGCGATCGAGGCCGCCGGCTACAAGGCCGGAGAGGACATCGTTCTCGCC
CTCGACGCCGCCTCGAGCGAATTCTACAAGGACGGCAAATACGTGCTGGCCGGCGAAGGCAAGACGCTCGATGCCGAAGG
CATGGTCAAGTACTACGCCGAGCTGTGCAAGCGCTACCCGATCCTGTCGATCGAGGACGGCTGCGCCGAGGACGATTGGG
CGGGCTGGTCGCTGCTGACCGCCGAGCTTGGCGCCAAGGTTCAGCTGGTCGGCGACGATCTGTTCGTGACCAACCCGCTG
CGTCTGGCCGAAGGCATCCGCAAGGGCGTGGCCAATTCCATCCTGGTCAAGGTCAACCAGATCGGCACCCTGTCGGAAAC
CCTGGAAGCCGTCGAGATGGCCCATAAGGCCGGCTATACCTCGGTGCTGTCGCATCGCTCGGGCGAGACCGAGGATTCCA
CCATCGCCGATATCGCCGTGGCCACCAACTGCGGCCAGATCAAGACCGGATCGCTGTCGCGCTCCGATCGTCTGGCGAAA
TACAACCAGTTGATCCGCATCGAAGAAGAACTGGGCCCCGTGGCCGTCTACGCCGGCGCCAGCATCCTGCGCGGTTAA

Upstream 100 bases:

>100_bases
TCGCGCTTGATGCCATGCCCGCGCTGATCGCGGTCTTGCGCGATCTTGATGCCTTTGCCAAAAAATACCCTGTAACCCTC
TGATATCAGGAGAGACCGTC

Downstream 100 bases:

>100_bases
TCGGTCTTCGGCCGTTGTTGGCCTGATTGGACAAAAAACGGGCCGCCGTTCCCTTGGGATGGCGGCCTTTTTTATCCGTC
GACGGCCGGCGGCAAGGGGA

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MAEIIDIHAREILDSRGNPTVEVDVLLDSGAFGRAAVPSGASTGAHEAVELRDGDKTRYAGKGVLKAVEAVNGELFSALS
GLDATDQLLIDQAMIDLDGTPNKARLGANAILGVSLACAKAAAEEAELPLYRYIGGARSHILPVPMMNIINGGQHADNPI
DVQEFMIMPVSAPTVADAVRMGAEVFHALKKKLKDAGHNTNVGDEGGFAPNLASADEALAFIVKAIEAAGYKAGEDIVLA
LDAASSEFYKDGKYVLAGEGKTLDAEGMVKYYAELCKRYPILSIEDGCAEDDWAGWSLLTAELGAKVQLVGDDLFVTNPL
RLAEGIRKGVANSILVKVNQIGTLSETLEAVEMAHKAGYTSVLSHRSGETEDSTIADIAVATNCGQIKTGSLSRSDRLAK
YNQLIRIEEELGPVAVYAGASILRG

Sequences:

>Translated_425_residues
MAEIIDIHAREILDSRGNPTVEVDVLLDSGAFGRAAVPSGASTGAHEAVELRDGDKTRYAGKGVLKAVEAVNGELFSALS
GLDATDQLLIDQAMIDLDGTPNKARLGANAILGVSLACAKAAAEEAELPLYRYIGGARSHILPVPMMNIINGGQHADNPI
DVQEFMIMPVSAPTVADAVRMGAEVFHALKKKLKDAGHNTNVGDEGGFAPNLASADEALAFIVKAIEAAGYKAGEDIVLA
LDAASSEFYKDGKYVLAGEGKTLDAEGMVKYYAELCKRYPILSIEDGCAEDDWAGWSLLTAELGAKVQLVGDDLFVTNPL
RLAEGIRKGVANSILVKVNQIGTLSETLEAVEMAHKAGYTSVLSHRSGETEDSTIADIAVATNCGQIKTGSLSRSDRLAK
YNQLIRIEEELGPVAVYAGASILRG
>Mature_424_residues
AEIIDIHAREILDSRGNPTVEVDVLLDSGAFGRAAVPSGASTGAHEAVELRDGDKTRYAGKGVLKAVEAVNGELFSALSG
LDATDQLLIDQAMIDLDGTPNKARLGANAILGVSLACAKAAAEEAELPLYRYIGGARSHILPVPMMNIINGGQHADNPID
VQEFMIMPVSAPTVADAVRMGAEVFHALKKKLKDAGHNTNVGDEGGFAPNLASADEALAFIVKAIEAAGYKAGEDIVLAL
DAASSEFYKDGKYVLAGEGKTLDAEGMVKYYAELCKRYPILSIEDGCAEDDWAGWSLLTAELGAKVQLVGDDLFVTNPLR
LAEGIRKGVANSILVKVNQIGTLSETLEAVEMAHKAGYTSVLSHRSGETEDSTIADIAVATNCGQIKTGSLSRSDRLAKY
NQLIRIEEELGPVAVYAGASILRG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=428, Percent_Identity=54.6728971962617, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI301897477, Length=425, Percent_Identity=53.8823529411765, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI301897469, Length=425, Percent_Identity=53.8823529411765, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI4503571, Length=431, Percent_Identity=52.4361948955916, Blast_Score=425, Evalue=1e-119,
Organism=Homo sapiens, GI301897479, Length=423, Percent_Identity=48.936170212766, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI169201331, Length=337, Percent_Identity=26.7062314540059, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI169201757, Length=337, Percent_Identity=26.7062314540059, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI239744207, Length=337, Percent_Identity=26.7062314540059, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=64.1860465116279, Blast_Score=530, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI71995829, Length=432, Percent_Identity=53.4722222222222, Blast_Score=423, Evalue=1e-119,
Organism=Caenorhabditis elegans, GI17536383, Length=435, Percent_Identity=53.1034482758621, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=49.7354497354497, Blast_Score=186, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6321693, Length=434, Percent_Identity=51.6129032258064, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323985, Length=431, Percent_Identity=51.9721577726218, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324974, Length=431, Percent_Identity=51.7401392111369, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6324969, Length=431, Percent_Identity=51.7401392111369, Blast_Score=409, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6321968, Length=434, Percent_Identity=50.9216589861751, Blast_Score=390, Evalue=1e-109,
Organism=Drosophila melanogaster, GI24580918, Length=434, Percent_Identity=52.5345622119816, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580916, Length=434, Percent_Identity=52.5345622119816, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580920, Length=434, Percent_Identity=52.5345622119816, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI24580914, Length=434, Percent_Identity=52.5345622119816, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI281360527, Length=434, Percent_Identity=52.5345622119816, Blast_Score=403, Evalue=1e-113,
Organism=Drosophila melanogaster, GI17137654, Length=434, Percent_Identity=52.5345622119816, Blast_Score=403, Evalue=1e-113,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_RHORT (Q2RT60)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426972.1
- HSSP:   P0A6P9
- ProteinModelPortal:   Q2RT60
- SMR:   Q2RT60
- STRING:   Q2RT60
- GeneID:   3835309
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1885
- NMPDR:   fig|1085.1.peg.2458
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- PhylomeDB:   Q2RT60
- ProtClustDB:   PRK00077
- BioCyc:   RRUB269796:RRU_A1885-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 44766; Mature: 44635

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 205-205 ACT_SITE 337-337 BINDING 155-155 BINDING 164-164 BINDING 285-285 BINDING 312-312 BINDING 337-337 BINDING 388-388

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIIDIHAREILDSRGNPTVEVDVLLDSGAFGRAAVPSGASTGAHEAVELRDGDKTRYA
CCCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVLKAVEAVNGELFSALSGLDATDQLLIDQAMIDLDGTPNKARLGANAILGVSLACAK
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAELPLYRYIGGARSHILPVPMMNIINGGQHADNPIDVQEFMIMPVSAPTVADAVR
HHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
MGAEVFHALKKKLKDAGHNTNVGDEGGFAPNLASADEALAFIVKAIEAAGYKAGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDAASSEFYKDGKYVLAGEGKTLDAEGMVKYYAELCKRYPILSIEDGCAEDDWAGWSLLT
EECCCHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHEE
AELGAKVQLVGDDLFVTNPLRLAEGIRKGVANSILVKVNQIGTLSETLEAVEMAHKAGYT
HHHCCEEEEECCCEEEECHHHHHHHHHHHCHHEEEEEECCCCCHHHHHHHHHHHHHCCHH
SVLSHRSGETEDSTIADIAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEELGPVAVYAGA
HHHHCCCCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHEEEHHHCCCEEEECCH
SILRG
HHHCC
>Mature Secondary Structure 
AEIIDIHAREILDSRGNPTVEVDVLLDSGAFGRAAVPSGASTGAHEAVELRDGDKTRYA
CCCHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCHHH
GKGVLKAVEAVNGELFSALSGLDATDQLLIDQAMIDLDGTPNKARLGANAILGVSLACAK
HHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHH
AAAEEAELPLYRYIGGARSHILPVPMMNIINGGQHADNPIDVQEFMIMPVSAPTVADAVR
HHHHHCCCCHHHHHCCCCCCCCCCCHHHHHCCCCCCCCCCCHHHEEEECCCCCHHHHHHH
MGAEVFHALKKKLKDAGHNTNVGDEGGFAPNLASADEALAFIVKAIEAAGYKAGEDIVLA
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEE
LDAASSEFYKDGKYVLAGEGKTLDAEGMVKYYAELCKRYPILSIEDGCAEDDWAGWSLLT
EECCCHHHHCCCCEEEECCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCHHHEE
AELGAKVQLVGDDLFVTNPLRLAEGIRKGVANSILVKVNQIGTLSETLEAVEMAHKAGYT
HHHCCEEEEECCCEEEECHHHHHHHHHHHCHHEEEEEECCCCCHHHHHHHHHHHHHCCHH
SVLSHRSGETEDSTIADIAVATNCGQIKTGSLSRSDRLAKYNQLIRIEEELGPVAVYAGA
HHHHCCCCCCCCCHHEEEEEECCCCCCCCCCCCCHHHHHHHHHHEEEHHHCCCEEEECCH
SILRG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA