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The map label for this gene is ntrC [H]

Identifier: 83593013

GI number: 83593013

Start: 1967836

End: 1969281

Strand: Direct

Name: ntrC [H]

Synonym: Rru_A1678

Alternate gene names: 83593013

Gene position: 1967836-1969281 (Clockwise)

Preceding gene: 83593012

Following gene: 83593014

Centisome position: 45.21

GC content: 64.52

Gene sequence:

>1446_bases
ATGTCGACGATTCTGGTGGCCGATGACGATCGCGGAATCCGCACGGTTCTCAGTCAGGCTCTGACCCGGGCCGGCCACGA
GGTGCGCAGCACCGGTACGGCCTCGACCCTGTGGAGCTGGGTCACCGATGGTCAGGGCGATCTGGTGATCACCGATGTCA
TCATGCCCGACGAGAACGGCCTCGACCTGATTCCGCGGATCAAGAAGATCCGCCCCGACCTGCGCATCGTCGTCATGAGC
GCCCAGAACACCTTGCTGACGGCGGTCAAGGCCACCGAGCGCGGCGCCTTTGAATATCTGCCCAAGCCCTTCGATTTGAC
CGAGGTGGTCAATGTCGTGCGCCGGGCCTTGTCGCTGCCCAGGGGCAATCCGACCGAGGGCGACCATGCTAGCGAGGAAG
ACGAGCGCCTGCCGCTGATCGGCCGCTCGCCGGCGATGCAGGAGATCTACCGCACCCTGGCCCGGCTGATGAACACCGAT
CTGACGGTGATGATCAACGGCGAATCGGGAACGGGCAAGGAACTGGTCGCCCGCGCCCTGCATGATTACGGCAAGCGCCG
CAACGGCTCGTTCGTCGCCGTCAACATGGGGGCGATCCCGCGCGAACTGATCGAAAGCGAATTGTTCGGCCACGAGAAGG
GCGCCTTCACCGGCGCCCATGCCCGCTCGGCCGGACGCTTCGAACAGGCCGATGGCGGCACCTTGTTCCTAGACGAGATC
GGCGACATGCCGCTGGAAGCGCAAACCCGGTTGCTGCGCGTGCTTCAGGAAAGCGAATACACCACCGTTGGCGGCCGCCA
GACCCTGCGGGCCAATGTGCGCATCATCGCCGCCACCCACCGCGACCTGCGCCAGCTTATTCATCAAGGATTGTTCCGCG
AGGATTTGTTCTATCGGCTCAACGTCGTGCCGCTGCGCCTGCCGCCTTTGCGCGAGCGCGTCGAGGACATCCCCGAACTG
ACCCGCCATTTCCTGCATGCGGTGGCCGAGCATGACGGCCTGCCGCCCAAGGTGCTGGAGGCCCCGGCGATGGAACGGCT
GAAAAAACACCGCTGGTCGGGCAATGTCCGCGAACTGGAAAATCTGGTGCGCCGTCTGGCCGCGCTCTATTCCCAGGAAG
TCATCGGCGTCGAGGTCATCGAGGCCGAATTGTCGTCTTCGGCGCCGCCGATCCTTGACGAGGGCGATGAATCGGCCGAG
GGGCTGTCGACCAAGGTCGAACGCCATCTTCGCCATTATTTCGAAGCCCATGGCGAGGCCCTGCCGCCGGCCGGCCTGCA
CGAGCGGATCAGCCGCGAGGTCGAGCGGCCGCTGATTTCCCTGTGTCTGGAGGCGACGCGCGGCAATCAGATCAAGGCCG
CCCAATTGCTCGGCCTCAACCGCAATACCCTGCGCAAGAAGATCCGGGATCTCGATATCCAGGTCGTGCGCGGCGTCAAG
GGCTAG

Upstream 100 bases:

>100_bases
GACGCCCAAGATCCCCGCCGACGCCGGCCAGCTCCGCGTCGGGCGCCCCCTTTGACATGTCTTCGGGCATGGGTTGAAAT
GAGTAAAGGAACCTTTTGAG

Downstream 100 bases:

>100_bases
GCCGCCTTGTCCCCCACCAGCCAGCGGCGCGGCGGCCGTCCCGCCCTCAACCGCACCATCTTGCGCCTGGGTCTGTGGGC
GCGGCGGGTCAAGCTGTCGC

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MSTILVADDDRGIRTVLSQALTRAGHEVRSTGTASTLWSWVTDGQGDLVITDVIMPDENGLDLIPRIKKIRPDLRIVVMS
AQNTLLTAVKATERGAFEYLPKPFDLTEVVNVVRRALSLPRGNPTEGDHASEEDERLPLIGRSPAMQEIYRTLARLMNTD
LTVMINGESGTGKELVARALHDYGKRRNGSFVAVNMGAIPRELIESELFGHEKGAFTGAHARSAGRFEQADGGTLFLDEI
GDMPLEAQTRLLRVLQESEYTTVGGRQTLRANVRIIAATHRDLRQLIHQGLFREDLFYRLNVVPLRLPPLRERVEDIPEL
TRHFLHAVAEHDGLPPKVLEAPAMERLKKHRWSGNVRELENLVRRLAALYSQEVIGVEVIEAELSSSAPPILDEGDESAE
GLSTKVERHLRHYFEAHGEALPPAGLHERISREVERPLISLCLEATRGNQIKAAQLLGLNRNTLRKKIRDLDIQVVRGVK
G

Sequences:

>Translated_481_residues
MSTILVADDDRGIRTVLSQALTRAGHEVRSTGTASTLWSWVTDGQGDLVITDVIMPDENGLDLIPRIKKIRPDLRIVVMS
AQNTLLTAVKATERGAFEYLPKPFDLTEVVNVVRRALSLPRGNPTEGDHASEEDERLPLIGRSPAMQEIYRTLARLMNTD
LTVMINGESGTGKELVARALHDYGKRRNGSFVAVNMGAIPRELIESELFGHEKGAFTGAHARSAGRFEQADGGTLFLDEI
GDMPLEAQTRLLRVLQESEYTTVGGRQTLRANVRIIAATHRDLRQLIHQGLFREDLFYRLNVVPLRLPPLRERVEDIPEL
TRHFLHAVAEHDGLPPKVLEAPAMERLKKHRWSGNVRELENLVRRLAALYSQEVIGVEVIEAELSSSAPPILDEGDESAE
GLSTKVERHLRHYFEAHGEALPPAGLHERISREVERPLISLCLEATRGNQIKAAQLLGLNRNTLRKKIRDLDIQVVRGVK
G
>Mature_480_residues
STILVADDDRGIRTVLSQALTRAGHEVRSTGTASTLWSWVTDGQGDLVITDVIMPDENGLDLIPRIKKIRPDLRIVVMSA
QNTLLTAVKATERGAFEYLPKPFDLTEVVNVVRRALSLPRGNPTEGDHASEEDERLPLIGRSPAMQEIYRTLARLMNTDL
TVMINGESGTGKELVARALHDYGKRRNGSFVAVNMGAIPRELIESELFGHEKGAFTGAHARSAGRFEQADGGTLFLDEIG
DMPLEAQTRLLRVLQESEYTTVGGRQTLRANVRIIAATHRDLRQLIHQGLFREDLFYRLNVVPLRLPPLRERVEDIPELT
RHFLHAVAEHDGLPPKVLEAPAMERLKKHRWSGNVRELENLVRRLAALYSQEVIGVEVIEAELSSSAPPILDEGDESAEG
LSTKVERHLRHYFEAHGEALPPAGLHERISREVERPLISLCLEATRGNQIKAAQLLGLNRNTLRKKIRDLDIQVVRGVKG

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=482, Percent_Identity=44.8132780082988, Blast_Score=379, Evalue=1e-106,
Organism=Escherichia coli, GI1788550, Length=477, Percent_Identity=38.5744234800839, Blast_Score=314, Evalue=9e-87,
Organism=Escherichia coli, GI1790437, Length=465, Percent_Identity=38.9247311827957, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1788905, Length=463, Percent_Identity=38.0129589632829, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI87082117, Length=231, Percent_Identity=53.6796536796537, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1789087, Length=338, Percent_Identity=42.3076923076923, Blast_Score=241, Evalue=8e-65,
Organism=Escherichia coli, GI1789233, Length=342, Percent_Identity=42.6900584795322, Blast_Score=238, Evalue=9e-64,
Organism=Escherichia coli, GI87082152, Length=242, Percent_Identity=47.5206611570248, Blast_Score=216, Evalue=4e-57,
Organism=Escherichia coli, GI1786524, Length=255, Percent_Identity=47.843137254902, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI87081872, Length=339, Percent_Identity=38.3480825958702, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI1787583, Length=227, Percent_Identity=39.647577092511, Blast_Score=175, Evalue=6e-45,
Organism=Escherichia coli, GI1789828, Length=287, Percent_Identity=32.7526132404181, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI87081858, Length=316, Percent_Identity=28.4810126582279, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1789809, Length=114, Percent_Identity=31.5789473684211, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 53655; Mature: 53524

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTILVADDDRGIRTVLSQALTRAGHEVRSTGTASTLWSWVTDGQGDLVITDVIMPDENG
CCEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCC
LDLIPRIKKIRPDLRIVVMSAQNTLLTAVKATERGAFEYLPKPFDLTEVVNVVRRALSLP
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHCCC
RGNPTEGDHASEEDERLPLIGRSPAMQEIYRTLARLMNTDLTVMINGESGTGKELVARAL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
HDYGKRRNGSFVAVNMGAIPRELIESELFGHEKGAFTGAHARSAGRFEQADGGTLFLDEI
HHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEHHHH
GDMPLEAQTRLLRVLQESEYTTVGGRQTLRANVRIIAATHRDLRQLIHQGLFREDLFYRL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECEEEEEECHHHHHHHHHHHHHHHHHHHEE
NVVPLRLPPLRERVEDIPELTRHFLHAVAEHDGLPPKVLEAPAMERLKKHRWSGNVRELE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCHHHHH
NLVRRLAALYSQEVIGVEVIEAELSSSAPPILDEGDESAEGLSTKVERHLRHYFEAHGEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LPPAGLHERISREVERPLISLCLEATRGNQIKAAQLLGLNRNTLRKKIRDLDIQVVRGVK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCHHHHCCCC
G
C
>Mature Secondary Structure 
STILVADDDRGIRTVLSQALTRAGHEVRSTGTASTLWSWVTDGQGDLVITDVIMPDENG
CEEEEECCCCCHHHHHHHHHHHCCHHHHCCCCHHHHHHHHCCCCCCEEEEEEEECCCCC
LDLIPRIKKIRPDLRIVVMSAQNTLLTAVKATERGAFEYLPKPFDLTEVVNVVRRALSLP
CCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHCCCHHHCCCCCCHHHHHHHHHHHHCCC
RGNPTEGDHASEEDERLPLIGRSPAMQEIYRTLARLMNTDLTVMINGESGTGKELVARAL
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHH
HDYGKRRNGSFVAVNMGAIPRELIESELFGHEKGAFTGAHARSAGRFEQADGGTLFLDEI
HHHHCCCCCCEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCEEEHHHH
GDMPLEAQTRLLRVLQESEYTTVGGRQTLRANVRIIAATHRDLRQLIHQGLFREDLFYRL
CCCCHHHHHHHHHHHHHCCCCCCCCCCEEEECEEEEEECHHHHHHHHHHHHHHHHHHHEE
NVVPLRLPPLRERVEDIPELTRHFLHAVAEHDGLPPKVLEAPAMERLKKHRWSGNVRELE
EEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHCCCCCHHHHH
NLVRRLAALYSQEVIGVEVIEAELSSSAPPILDEGDESAEGLSTKVERHLRHYFEAHGEA
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCC
LPPAGLHERISREVERPLISLCLEATRGNQIKAAQLLGLNRNTLRKKIRDLDIQVVRGVK
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCHHHHHHHHHHCCHHHHCCCC
G
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7553451 [H]