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The map label for this gene is leuD

Identifier: 83592526

GI number: 83592526

Start: 1407685

End: 1408308

Strand: Direct

Name: leuD

Synonym: Rru_A1190

Alternate gene names: 83592526

Gene position: 1407685-1408308 (Clockwise)

Preceding gene: 83592525

Following gene: 83592527

Centisome position: 32.34

GC content: 60.58

Gene sequence:

>624_bases
ATGGAAAAATTCACCGTGCTGACCGGGATCGCGGCGCCTTTGCCGATGATCAATATCGATACCGACATGATCATTCCCAA
GCAGTTCCTGAAGACCATCAAGCGGACGGGCCTGGGCAAGAACCTGTTTGACGAGATGCGGTTTCTGGAAGACGGCGCCG
AGAACCCGGATTTCATTCTGAACAAGGCCGCTTGGCGCAAGGCGACCATCCTGGTGACCGGGGCCAATTTCGGCTGCGGC
AGCTCGCGCGAACATGCCCCCTGGGCCCTGCTCGATTTCGGCATCCGCTGCGTCATCGCCCCCAGCTTCGCCGATATCTT
TTTCAACAACTGCTTCAAGAACGGCATCCTGCCCATCGCCCTGCCGCAGGTCGAGATCGACAAGCTGCTCGACGACGCCG
GGCGCGGCTCCAACGCCACGCTGACGGTCGATCTGGAAAATCAGGTGATCCGCGGCCCCGATGGCGGCCAGATCGCCTTC
GAGATCGACGCCTTCCGCAAGCATTGTCTGCTCAACGGTCTGGACGATATCGGCCTGACCTTGCAGAAGGGCGACAAGAT
CGACGATTTCGAGCGGACGCGGGCGATGAGTTCGCCCTGGCTGCCGATCGCCAAAGCCGTCTGA

Upstream 100 bases:

>100_bases
AATCCCTGGCCCCCTCGCTGGGGTGACGGCTCCCCCCTGTCACGGGGGGGATCGGCCAGGGCGGCGGGCTTTGATGCGAG
ATGAGATGAGGGTTTGAGTG

Downstream 100 bases:

>100_bases
TCCGTTCAAGGGGCGCGCCGCCGGGTGCGCCCTTTTTCATACCGTCTCCGCTCTTGTGACGAACGATTTTTCCAAGGACA
TTTTCCATGCCCGTTTCCAA

Product: isopropylmalate isomerase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MEKFTVLTGIAAPLPMINIDTDMIIPKQFLKTIKRTGLGKNLFDEMRFLEDGAENPDFILNKAAWRKATILVTGANFGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIALPQVEIDKLLDDAGRGSNATLTVDLENQVIRGPDGGQIAF
EIDAFRKHCLLNGLDDIGLTLQKGDKIDDFERTRAMSSPWLPIAKAV

Sequences:

>Translated_207_residues
MEKFTVLTGIAAPLPMINIDTDMIIPKQFLKTIKRTGLGKNLFDEMRFLEDGAENPDFILNKAAWRKATILVTGANFGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIALPQVEIDKLLDDAGRGSNATLTVDLENQVIRGPDGGQIAF
EIDAFRKHCLLNGLDDIGLTLQKGDKIDDFERTRAMSSPWLPIAKAV
>Mature_207_residues
MEKFTVLTGIAAPLPMINIDTDMIIPKQFLKTIKRTGLGKNLFDEMRFLEDGAENPDFILNKAAWRKATILVTGANFGCG
SSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIALPQVEIDKLLDDAGRGSNATLTVDLENQVIRGPDGGQIAF
EIDAFRKHCLLNGLDDIGLTLQKGDKIDDFERTRAMSSPWLPIAKAV

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786258, Length=192, Percent_Identity=51.0416666666667, Blast_Score=201, Evalue=4e-53,
Organism=Saccharomyces cerevisiae, GI6321429, Length=208, Percent_Identity=58.6538461538462, Blast_Score=223, Evalue=1e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEUD_RHORT (Q2RV54)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426278.1
- ProteinModelPortal:   Q2RV54
- SMR:   Q2RV54
- STRING:   Q2RV54
- GeneID:   3834700
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1190
- NMPDR:   fig|1085.1.peg.3176
- eggNOG:   COG0066
- HOGENOM:   HBG304838
- OMA:   DEISITM
- PhylomeDB:   Q2RV54
- ProtClustDB:   PRK01641
- BioCyc:   RRUB269796:RRU_A1190-MONOMER
- HAMAP:   MF_01031
- InterPro:   IPR004431
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.20.19.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- TIGRFAMs:   TIGR00171

Pfam domain/function: PF00694 Aconitase_C; SSF52016 Aconitase/3IPM_dehydase_swvl

EC number: =4.2.1.33

Molecular weight: Translated: 22808; Mature: 22808

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFTVLTGIAAPLPMINIDTDMIIPKQFLKTIKRTGLGKNLFDEMRFLEDGAENPDFIL
CCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEE
NKAAWRKATILVTGANFGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIA
ECCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEE
LPQVEIDKLLDDAGRGSNATLTVDLENQVIRGPDGGQIAFEIDAFRKHCLLNGLDDIGLT
ECCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LQKGDKIDDFERTRAMSSPWLPIAKAV
EECCCCCCHHHHHHHCCCCCCCHHCCC
>Mature Secondary Structure
MEKFTVLTGIAAPLPMINIDTDMIIPKQFLKTIKRTGLGKNLFDEMRFLEDGAENPDFIL
CCCEEEEECCCCCCCEEECCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCEE
NKAAWRKATILVTGANFGCGSSREHAPWALLDFGIRCVIAPSFADIFFNNCFKNGILPIA
ECCCCCEEEEEEECCCCCCCCCCCCCCCHHHCCCEEEEECCHHHHHHHHHHHHCCCCEEE
LPQVEIDKLLDDAGRGSNATLTVDLENQVIRGPDGGQIAFEIDAFRKHCLLNGLDDIGLT
ECCHHHHHHHHHCCCCCCCEEEEEECCCEEECCCCCEEEEEHHHHHHHHHHCCCCCCCEE
LQKGDKIDDFERTRAMSSPWLPIAKAV
EECCCCCCHHHHHHHCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA