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The map label for this gene is leuB

Identifier: 83592527

GI number: 83592527

Start: 1408395

End: 1409507

Strand: Direct

Name: leuB

Synonym: Rru_A1191

Alternate gene names: 83592527

Gene position: 1408395-1409507 (Clockwise)

Preceding gene: 83592526

Following gene: 83592531

Centisome position: 32.36

GC content: 66.67

Gene sequence:

>1113_bases
ATGCCCGTTTCCAAGAAGATCCTGTTCCTTGCCGGCGACGGCATCGGCCCCGAAGTCATGGCCCAGGTGCGCCGGGTCAT
GGACTGGCTGAACCAAACCGGCCGCACCGCCTTTGAAGTCGAAGACGCCTTGGTTGGCGGCGCCGCCTATGACGCCCAGG
GCACGCCCCTGCATGACGACACCGTCGCCCTGGCCCTGGCCGCCGACGCCGTGCTGCTGGGCGCCGTCGGCGGGCCGAAA
TGGGATGCCTCCCTGCCTTTCGAGCTTAAGCCCGAGCGTGGGCTTTTGCGCCTGCGCAAGGACCTGGAGCTGTTTGCCAA
TCTGCGTCCGGCGATGGTCTTCGACGCCCTGGCCGAGGCTTCGACCCTGAAGACCGAGCTGGTCTCGGGCCTTGACGTGA
TGATCGTGCGCGAGCTGACCGGCGGCGTTTATTTCGGCCAGCCGCGCGGCATCAGCGCCCTGCCCAACGGCGAGCGCCGC
GGCGTCAACACCCAGGTCTATGACACCCACGAGATCGTGCGCGTCGCCAAGGTGGCCTTCGATCTGGCGGGCAAGCGCGG
CAAGCGGCTGTGCAGCGTCGAAAAGGCCAATGTCATGGAAAGCGGCCTGCTGTGGCGCGAGGAGGTGACCAAGCTGGGCG
CCGACTACCCCGAGATCGCCCTGTCGCATATGTACGCCGATAACTGCGCCATGCAGTTGGTCCGCCAGCCCAAGCAGTTC
GATGTCATCGTCACCGACAACCTGTTTGGCGATATCCTGTCCGATTGCGCCGCCATGCTCACCGGGTCGCTGGGCATGCT
GCCCTCGGCCAGCCTGGGCGCCGCCGACGCGGCGGGCAACCGTCGGGCGATGTACGAACCGGTCCATGGCTCGGCCCCCG
ATATCGCCGGCCAGGACAAGGCCAATCCGCTGGCGACCATCCTCAGCTTCTCGATGATGCTGCGCTACAGCTTCGATCTG
GCCGAAGAGGCCGATCTGGTCGACAACGCCGTGCGGGGCGTTCTGGCCAAGGGCCTGCGCACCGGCGACATCTTCCAGCC
CGGCAAGACCCTGGTCGGCACCACCGGCATGGGCGACGCCCTGCTGGCCGAGATGGCCCAATTGGCCAACTAA

Upstream 100 bases:

>100_bases
CCAAAGCCGTCTGATCCGTTCAAGGGGCGCGCCGCCGGGTGCGCCCTTTTTCATACCGTCTCCGCTCTTGTGACGAACGA
TTTTTCCAAGGACATTTTCC

Downstream 100 bases:

>100_bases
TCCAGGGCTTTCCGCTCCGAAAACACACAGGCCCCCGGGGATCGCTCCGGGGGCCTGTGGTGTTTCGGAAGCAAATCCCG
AGCACTCTGGATCAGAGTGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH

Number of amino acids: Translated: 370; Mature: 369

Protein sequence:

>370_residues
MPVSKKILFLAGDGIGPEVMAQVRRVMDWLNQTGRTAFEVEDALVGGAAYDAQGTPLHDDTVALALAADAVLLGAVGGPK
WDASLPFELKPERGLLRLRKDLELFANLRPAMVFDALAEASTLKTELVSGLDVMIVRELTGGVYFGQPRGISALPNGERR
GVNTQVYDTHEIVRVAKVAFDLAGKRGKRLCSVEKANVMESGLLWREEVTKLGADYPEIALSHMYADNCAMQLVRQPKQF
DVIVTDNLFGDILSDCAAMLTGSLGMLPSASLGAADAAGNRRAMYEPVHGSAPDIAGQDKANPLATILSFSMMLRYSFDL
AEEADLVDNAVRGVLAKGLRTGDIFQPGKTLVGTTGMGDALLAEMAQLAN

Sequences:

>Translated_370_residues
MPVSKKILFLAGDGIGPEVMAQVRRVMDWLNQTGRTAFEVEDALVGGAAYDAQGTPLHDDTVALALAADAVLLGAVGGPK
WDASLPFELKPERGLLRLRKDLELFANLRPAMVFDALAEASTLKTELVSGLDVMIVRELTGGVYFGQPRGISALPNGERR
GVNTQVYDTHEIVRVAKVAFDLAGKRGKRLCSVEKANVMESGLLWREEVTKLGADYPEIALSHMYADNCAMQLVRQPKQF
DVIVTDNLFGDILSDCAAMLTGSLGMLPSASLGAADAAGNRRAMYEPVHGSAPDIAGQDKANPLATILSFSMMLRYSFDL
AEEADLVDNAVRGVLAKGLRTGDIFQPGKTLVGTTGMGDALLAEMAQLAN
>Mature_369_residues
PVSKKILFLAGDGIGPEVMAQVRRVMDWLNQTGRTAFEVEDALVGGAAYDAQGTPLHDDTVALALAADAVLLGAVGGPKW
DASLPFELKPERGLLRLRKDLELFANLRPAMVFDALAEASTLKTELVSGLDVMIVRELTGGVYFGQPRGISALPNGERRG
VNTQVYDTHEIVRVAKVAFDLAGKRGKRLCSVEKANVMESGLLWREEVTKLGADYPEIALSHMYADNCAMQLVRQPKQFD
VIVTDNLFGDILSDCAAMLTGSLGMLPSASLGAADAAGNRRAMYEPVHGSAPDIAGQDKANPLATILSFSMMLRYSFDLA
EEADLVDNAVRGVLAKGLRTGDIFQPGKTLVGTTGMGDALLAEMAQLAN

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5031777, Length=320, Percent_Identity=31.25, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI28178821, Length=339, Percent_Identity=31.858407079646, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI28178816, Length=339, Percent_Identity=31.858407079646, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI4758582, Length=350, Percent_Identity=29.4285714285714, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28178838, Length=315, Percent_Identity=29.8412698412698, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI28178819, Length=165, Percent_Identity=40, Blast_Score=101, Evalue=1e-21,
Organism=Escherichia coli, GI87081683, Length=369, Percent_Identity=46.8834688346883, Blast_Score=322, Evalue=2e-89,
Organism=Escherichia coli, GI1788101, Length=352, Percent_Identity=32.1022727272727, Blast_Score=158, Evalue=6e-40,
Organism=Escherichia coli, GI1787381, Length=312, Percent_Identity=28.2051282051282, Blast_Score=91, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71986051, Length=368, Percent_Identity=33.1521739130435, Blast_Score=155, Evalue=3e-38,
Organism=Caenorhabditis elegans, GI17550882, Length=345, Percent_Identity=31.8840579710145, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17505779, Length=341, Percent_Identity=29.3255131964809, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI25144293, Length=346, Percent_Identity=29.7687861271676, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=374, Percent_Identity=40.1069518716578, Blast_Score=268, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=324, Percent_Identity=35.4938271604938, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=33.1412103746398, Blast_Score=138, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6324291, Length=345, Percent_Identity=30.4347826086957, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24643270, Length=349, Percent_Identity=34.3839541547278, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24643268, Length=349, Percent_Identity=34.3839541547278, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24661184, Length=352, Percent_Identity=31.8181818181818, Blast_Score=115, Evalue=6e-26,
Organism=Drosophila melanogaster, GI281362242, Length=350, Percent_Identity=29.1428571428571, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI24648872, Length=350, Percent_Identity=29.1428571428571, Blast_Score=107, Evalue=9e-24,
Organism=Drosophila melanogaster, GI161078633, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24650122, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078635, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078637, Length=347, Percent_Identity=27.0893371757925, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078639, Length=345, Percent_Identity=27.2463768115942, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI20130355, Length=348, Percent_Identity=25.2873563218391, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU3_RHORT (Q2RV53)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426279.1
- HSSP:   Q9WZ26
- ProteinModelPortal:   Q2RV53
- SMR:   Q2RV53
- STRING:   Q2RV53
- GeneID:   3834701
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1191
- NMPDR:   fig|1085.1.peg.3175
- eggNOG:   COG0473
- HOGENOM:   HBG518924
- OMA:   AMQLVKW
- PhylomeDB:   Q2RV53
- ProtClustDB:   PRK00772
- BioCyc:   RRUB269796:RRU_A1191-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01033
- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429
- Gene3D:   G3DSA:3.40.718.10
- PANTHER:   PTHR11835
- PANTHER:   PTHR11835:SF13
- TIGRFAMs:   TIGR00169

Pfam domain/function: PF00180 Iso_dh

EC number: =1.1.1.85

Molecular weight: Translated: 39537; Mature: 39406

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00470 IDH_IMDH

Important sites: BINDING 99-99 BINDING 109-109 BINDING 137-137 BINDING 227-227

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVSKKILFLAGDGIGPEVMAQVRRVMDWLNQTGRTAFEVEDALVGGAAYDAQGTPLHDD
CCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEECCCCCCCCCH
TVALALAADAVLLGAVGGPKWDASLPFELKPERGLLRLRKDLELFANLRPAMVFDALAEA
HHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
STLKTELVSGLDVMIVRELTGGVYFGQPRGISALPNGERRGVNTQVYDTHEIVRVAKVAF
HHHHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
DLAGKRGKRLCSVEKANVMESGLLWREEVTKLGADYPEIALSHMYADNCAMQLVRQPKQF
HHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
DVIVTDNLFGDILSDCAAMLTGSLGMLPSASLGAADAAGNRRAMYEPVHGSAPDIAGQDK
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
ANPLATILSFSMMLRYSFDLAEEADLVDNAVRGVLAKGLRTGDIFQPGKTLVGTTGMGDA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHH
LLAEMAQLAN
HHHHHHHHCC
>Mature Secondary Structure 
PVSKKILFLAGDGIGPEVMAQVRRVMDWLNQTGRTAFEVEDALVGGAAYDAQGTPLHDD
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEEHHHHHHCCCEECCCCCCCCCH
TVALALAADAVLLGAVGGPKWDASLPFELKPERGLLRLRKDLELFANLRPAMVFDALAEA
HHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHH
STLKTELVSGLDVMIVRELTGGVYFGQPRGISALPNGERRGVNTQVYDTHEIVRVAKVAF
HHHHHHHHCCCHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHH
DLAGKRGKRLCSVEKANVMESGLLWREEVTKLGADYPEIALSHMYADNCAMQLVRQPKQF
HHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCE
DVIVTDNLFGDILSDCAAMLTGSLGMLPSASLGAADAAGNRRAMYEPVHGSAPDIAGQDK
EEEEECHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCC
ANPLATILSFSMMLRYSFDLAEEADLVDNAVRGVLAKGLRTGDIFQPGKTLVGTTGMGDA
CCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCHH
LLAEMAQLAN
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA