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The map label for this gene is leuC

Identifier: 83592525

GI number: 83592525

Start: 1406192

End: 1407610

Strand: Direct

Name: leuC

Synonym: Rru_A1189

Alternate gene names: 83592525

Gene position: 1406192-1407610 (Clockwise)

Preceding gene: 83592524

Following gene: 83592526

Centisome position: 32.31

GC content: 66.03

Gene sequence:

>1419_bases
ATGGGTAAGCCCAAAACCCTGTTCAACAAGATCTGGCAAAGCCATCTCGTGGACGTGCAGGATGATGGGACCTGCCTGAT
TTACATCGATCGCCATCTGGTTCACGAAGTGACCAGCCCCCAGGCTTTCGAAGGCCTGCGGATGACCGGACGCAAGGTGC
GCCGGCCCGAGCAGACCCTGGCGGTGGCCGATCATAACGTGCCCACCACCGATCGCTCCAAGGGCATCGCCGACGAGGAA
AGCCGCATCCAGGTCGAGGCTTTGGGCAGCAATACCGCCGAATTCGGCGTGCCCTATCTGGAAATGGACGATATCCGCCA
GGGCATCGTTCATATCGTCGGTCCCGAACAGGGCTTTACCCTGCCGGGGGCGACCATCGTCTGCGGCGATTCCCATACCT
CGACCCATGGCGCCTTCGGCGCTTTGGCCTTCGGGATCGGCACCAGCGAGGTCGAACACGTTCTGGCCACCCAGACGCTG
CTGCAGAAGCCGGCCAAGGACATGCTGGTGCGCATCGACGGGGCCTTGCGCCCGGGCGTGAGCGCCAAGGACATCGTGCT
CGCCGTGATCGGCCGCATCGGCACCGCCGGCGGCACCGGCCATGTGATCGAATTCGCCGGCGAGGCGATCCGCGGCCTGT
CGATGGAAGGCCGGATGACGGTCTGCAATATGACCATCGAAGGCGGCGCCCGCGCCGGGTTGATCGCCCCTGACGAGGTG
ACCTTCGAGTATCTGAAGGGGCGGCCCTTCGCCCCCAAGGGCGCGGCCTGGGAAGCGGCGATTTCCTATTGGAAGACGCT
GAAGACCGACGAGGGCGCGGTCTATGACACGGTTATCGAGATGGATGCCGGCGCGATCGAGCCGCAGGTGACCTGGGGGA
CCAGCCCGGAGAACGTGGTGGCGCTGTCGGGCCGGGTGCCCGATCCCGCCGCCGAGGCCGATCCTGGCCGCCGCGCCGCC
ATTGAGCGCGCCCTGGCCTATATGGATCTCAAGCCCGGCATGCCGATGACCGAGGTCGCCATCGACAAGGTGTTCATCGG
CTCGTGCACCAATGGCCGCATCGAAGACCTGCGCGCCGCCGCCGCCATCGCCAAGGGCCGCAAGGTGGCCGCCACCGTCA
ACGCCATCGTCGTTCCGGGATCGGGTCTGGTCAAGGAACAGGCCGAATCCGAAGGTCTGGACCAGATCTTCCTGGAGGCC
GGCTTTGAATGGCGCGAGCCGGGCTGCTCGATGTGTCTGGCGATGAACGCCGACAAGTTGGCGCCGGGCGAGCGCTGTGC
CTCGACCTCGAACCGCAATTTCGAAGGCCGCCAGGGCAAGGGGGGGCGGACCCATCTGGTCAGTCCGGCCGTCGCTGTCG
CCTCGGCGATCACCGGCAAGCTGACCGACGCCCAATCCCTGGCCCCCTCGCTGGGGTGA

Upstream 100 bases:

>100_bases
TGTCGCCGGGGTTTTCCCGGCGGCAGCCCCGGCGGGGACTGACCGCCTTTCCGTCTTGGATTTATGCGAACAGCCGAGCC
TTTATTTGGAGCGTCGATGC

Downstream 100 bases:

>100_bases
CGGCTCCCCCCTGTCACGGGGGGGATCGGCCAGGGCGGCGGGCTTTGATGCGAGATGAGATGAGGGTTTGAGTGATGGAA
AAATTCACCGTGCTGACCGG

Product: isopropylmalate isomerase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MGKPKTLFNKIWQSHLVDVQDDGTCLIYIDRHLVHEVTSPQAFEGLRMTGRKVRRPEQTLAVADHNVPTTDRSKGIADEE
SRIQVEALGSNTAEFGVPYLEMDDIRQGIVHIVGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
LQKPAKDMLVRIDGALRPGVSAKDIVLAVIGRIGTAGGTGHVIEFAGEAIRGLSMEGRMTVCNMTIEGGARAGLIAPDEV
TFEYLKGRPFAPKGAAWEAAISYWKTLKTDEGAVYDTVIEMDAGAIEPQVTWGTSPENVVALSGRVPDPAAEADPGRRAA
IERALAYMDLKPGMPMTEVAIDKVFIGSCTNGRIEDLRAAAAIAKGRKVAATVNAIVVPGSGLVKEQAESEGLDQIFLEA
GFEWREPGCSMCLAMNADKLAPGERCASTSNRNFEGRQGKGGRTHLVSPAVAVASAITGKLTDAQSLAPSLG

Sequences:

>Translated_472_residues
MGKPKTLFNKIWQSHLVDVQDDGTCLIYIDRHLVHEVTSPQAFEGLRMTGRKVRRPEQTLAVADHNVPTTDRSKGIADEE
SRIQVEALGSNTAEFGVPYLEMDDIRQGIVHIVGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTL
LQKPAKDMLVRIDGALRPGVSAKDIVLAVIGRIGTAGGTGHVIEFAGEAIRGLSMEGRMTVCNMTIEGGARAGLIAPDEV
TFEYLKGRPFAPKGAAWEAAISYWKTLKTDEGAVYDTVIEMDAGAIEPQVTWGTSPENVVALSGRVPDPAAEADPGRRAA
IERALAYMDLKPGMPMTEVAIDKVFIGSCTNGRIEDLRAAAAIAKGRKVAATVNAIVVPGSGLVKEQAESEGLDQIFLEA
GFEWREPGCSMCLAMNADKLAPGERCASTSNRNFEGRQGKGGRTHLVSPAVAVASAITGKLTDAQSLAPSLG
>Mature_471_residues
GKPKTLFNKIWQSHLVDVQDDGTCLIYIDRHLVHEVTSPQAFEGLRMTGRKVRRPEQTLAVADHNVPTTDRSKGIADEES
RIQVEALGSNTAEFGVPYLEMDDIRQGIVHIVGPEQGFTLPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLL
QKPAKDMLVRIDGALRPGVSAKDIVLAVIGRIGTAGGTGHVIEFAGEAIRGLSMEGRMTVCNMTIEGGARAGLIAPDEVT
FEYLKGRPFAPKGAAWEAAISYWKTLKTDEGAVYDTVIEMDAGAIEPQVTWGTSPENVVALSGRVPDPAAEADPGRRAAI
ERALAYMDLKPGMPMTEVAIDKVFIGSCTNGRIEDLRAAAAIAKGRKVAATVNAIVVPGSGLVKEQAESEGLDQIFLEAG
FEWREPGCSMCLAMNADKLAPGERCASTSNRNFEGRQGKGGRTHLVSPAVAVASAITGKLTDAQSLAPSLG

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 1 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=398, Percent_Identity=27.3869346733668, Blast_Score=108, Evalue=9e-24,
Organism=Homo sapiens, GI41352693, Length=383, Percent_Identity=27.154046997389, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI8659555, Length=403, Percent_Identity=26.7990074441687, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786259, Length=463, Percent_Identity=66.9546436285097, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1787531, Length=367, Percent_Identity=27.5204359673025, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI87081781, Length=351, Percent_Identity=26.4957264957265, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI2367097, Length=359, Percent_Identity=27.0194986072423, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI25149337, Length=481, Percent_Identity=27.8586278586279, Blast_Score=128, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI32564738, Length=362, Percent_Identity=30.3867403314917, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI25149342, Length=300, Percent_Identity=30.3333333333333, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568399, Length=401, Percent_Identity=25.4364089775561, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6321429, Length=471, Percent_Identity=62.208067940552, Blast_Score=619, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6320440, Length=463, Percent_Identity=28.0777537796976, Blast_Score=142, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6323335, Length=385, Percent_Identity=28.5714285714286, Blast_Score=133, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6322261, Length=368, Percent_Identity=28.5326086956522, Blast_Score=129, Evalue=1e-30,
Organism=Drosophila melanogaster, GI281365315, Length=479, Percent_Identity=26.9311064718163, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864292, Length=479, Percent_Identity=26.9311064718163, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI161076999, Length=384, Percent_Identity=28.125, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28571643, Length=479, Percent_Identity=26.9311064718163, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17137564, Length=371, Percent_Identity=25.3369272237197, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24645686, Length=369, Percent_Identity=26.2872628726287, Blast_Score=81, Evalue=2e-15,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_RHORT (Q2RV55)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_426277.1
- STRING:   Q2RV55
- GeneID:   3834699
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A1189
- NMPDR:   fig|1085.1.peg.3177
- eggNOG:   COG0065
- HOGENOM:   HBG330745
- OMA:   RPHAPKG
- PhylomeDB:   Q2RV55
- ProtClustDB:   PRK05478
- BioCyc:   RRUB269796:RRU_A1189-MONOMER
- HAMAP:   MF_01026
- InterPro:   IPR004430
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR015936
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR00170

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 49965; Mature: 49834

Theoretical pI: Translated: 5.35; Mature: 5.35

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKPKTLFNKIWQSHLVDVQDDGTCLIYIDRHLVHEVTSPQAFEGLRMTGRKVRRPEQTL
CCCCHHHHHHHHHHHCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCHHHCCCCHHE
AVADHNVPTTDRSKGIADEESRIQVEALGSNTAEFGVPYLEMDDIRQGIVHIVGPEQGFT
EEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCEEHHHHHCCEEEEECCCCCCC
LPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKPAKDMLVRIDGALRPGV
CCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCC
SAKDIVLAVIGRIGTAGGTGHVIEFAGEAIRGLSMEGRMTVCNMTIEGGARAGLIAPDEV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHH
TFEYLKGRPFAPKGAAWEAAISYWKTLKTDEGAVYDTVIEMDAGAIEPQVTWGTSPENVV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEE
ALSGRVPDPAAEADPGRRAAIERALAYMDLKPGMPMTEVAIDKVFIGSCTNGRIEDLRAA
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHH
AAIAKGRKVAATVNAIVVPGSGLVKEQAESEGLDQIFLEAGFEWREPGCSMCLAMNADKL
HHHHCCCEEEEEEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECCCCC
APGERCASTSNRNFEGRQGKGGRTHLVSPAVAVASAITGKLTDAQSLAPSLG
CCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHCCCCC
>Mature Secondary Structure 
GKPKTLFNKIWQSHLVDVQDDGTCLIYIDRHLVHEVTSPQAFEGLRMTGRKVRRPEQTL
CCCHHHHHHHHHHHCCEECCCCEEEEEECHHHHHHCCCCHHHHHHHHCCHHHCCCCHHE
AVADHNVPTTDRSKGIADEESRIQVEALGSNTAEFGVPYLEMDDIRQGIVHIVGPEQGFT
EEECCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHCCCCEEHHHHHCCEEEEECCCCCCC
LPGATIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTLLQKPAKDMLVRIDGALRPGV
CCCCEEEECCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHEEEECCCCCCCC
SAKDIVLAVIGRIGTAGGTGHVIEFAGEAIRGLSMEGRMTVCNMTIEGGARAGLIAPDEV
CHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCCCHH
TFEYLKGRPFAPKGAAWEAAISYWKTLKTDEGAVYDTVIEMDAGAIEPQVTWGTSPENVV
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCEEECCCCCCCEE
ALSGRVPDPAAEADPGRRAAIERALAYMDLKPGMPMTEVAIDKVFIGSCTNGRIEDLRAA
EEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHEEEECCCCCHHHHHHHH
AAIAKGRKVAATVNAIVVPGSGLVKEQAESEGLDQIFLEAGFEWREPGCSMCLAMNADKL
HHHHCCCEEEEEEEEEEECCCCHHHHHHHHCCHHHHHHHCCCCCCCCCCCEEEEECCCCC
APGERCASTSNRNFEGRQGKGGRTHLVSPAVAVASAITGKLTDAQSLAPSLG
CCCHHHCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHCCCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA