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The map label for this gene is eutB [H]

Identifier: 83592368

GI number: 83592368

Start: 1221288

End: 1222679

Strand: Reverse

Name: eutB [H]

Synonym: Rru_A1032

Alternate gene names: 83592368

Gene position: 1222679-1221288 (Counterclockwise)

Preceding gene: 83592369

Following gene: 83592367

Centisome position: 28.09

GC content: 67.1

Gene sequence:

>1392_bases
ATGGGATTGTATCGGGCGACGGTGGGGGGAACACGCTACGACTTCGCCGATTTGCGAACGGTGATGGCCTGCGCCTCGCC
GCGCCGCTCGGGCGACGAACTGGCCGGACTGGCCGCCGAGAGCGACGCCCAGCGCATGGCGGCCCGCCTCGTTCTCGCCG
ATCTGCCCCTGCGCGCCTTCCTTGACACGCCGCTTGTTCCCTATGAGAGCGACGAAGTCACCCGGCTGATCATCGATACC
CACGATGGCGCCGCCTTCGCCCCGGTCGCCAGCCTGACCGTTGGCGGCTTTCGCGACTGGCTGCTGTCCTATGCCGCCGA
TAGCGCCGCCCTTGCCGCCCTCGCCCCCGGACTGACCCCGGAAATGGTCGCCGCCGTCAGCAAGCTGATGCGCAACGCCG
ATCTGATCGCCGTGGCGGCGAAATGCCAGGTGATCACCGGCTTTCGCACGACGCTGGGCTTGCCCGGCCGTCTGGCCAGC
CGCCTTCAGCCCAACCACCCCACCGATGATCCCGCCGGTATCGCCGCCTCGACCCTTGACGGTCTGCTGTTTGGCATGGG
CGACGCGGTGATCGGCATCAATCCGGCGACCGATAACGTCGGCGCCTGCGTCACCTTGCTTGAGATGCTGGACGCCGTGC
GCCAGCGCTTCGACATCCCCAGCCAATCTTGCGTGCTGACCCATGTCACCAACAGCATCGAGGCGATCAACCGCGGCGCC
CCCCTCGACCTTGTCTTCCAGTCGGTCGCCGGCACCGAGGCGGCCAATGCCGGCTTTGGCATCAGCTTGTCGCTGCTGGG
CGAAGCCCGCGAGGCGGCCTTGTCGCTGCGGCGCGGCACGGTGGGCGACAACGTCATGTATTTCGAGACCGGCCAGGGCG
CGGCGCTGTCGGCCGACGCCCACCACGGCGTCGATCAGCAAACCGTCGAGGTCCGCGCCTATGCGGTCTGCCGGGCCTTC
AAGCCGCTGATCGTCAATACGGTCGTCGGCTTTATCGGCCCGGAATATCTGTATGATTCCAAGCAGATCATCCGCGCCGG
CCTGGAAGATCACTGCTGCGGCAAGCTGCTTGGCCTGCCGATGGGGGTGGATGTCTGCTACACCAACCACGCCGAGGCCG
ATCAGGACGATATGGATACCCTGATGACCCTGCTTGGCGTCGCCGGGGTCACCTTCCTGATCGGCGTGCCCGGCGCCGAT
GACGTGATGCTCAATTATCAGAGCCTGTCCTATCACGACATCCTTGGCCTGCGTCATCTGCTTGACCGCCGCCCCGCCCC
CGAATTCGCCGACTGGCTCGCGCGCATGGGCATGAGCGACGCCGGCGGACGCCTGCCGCCGCTCGATGCCAGCGCCCCGG
CCCTGCGTCGCCTTCTGGCTTCGGGAGGCTGA

Upstream 100 bases:

>100_bases
ATGTCAAGTCGCTCTGGTCCGACCCGGACCAGAATAAGCGCGGTCGCACCGTCTGGCCCCAGGCTTGGGACGGTCCGCCA
AGGCAGGGGGGACGGGAACG

Downstream 100 bases:

>100_bases
GACGGCCATGACCCTTCCCCCCGTCGTCGATCCCTTCGCCCGCTTCCGCGCCGCCACCCGCGCCCGGGTCGGCCTGGGGC
GCAGCGGTGACGCCCTGCCG

Product: ethanolamine ammonia-lyase heavy chain

Products: NA

Alternate protein names: Ethanolamine ammonia-lyase large subunit [H]

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MGLYRATVGGTRYDFADLRTVMACASPRRSGDELAGLAAESDAQRMAARLVLADLPLRAFLDTPLVPYESDEVTRLIIDT
HDGAAFAPVASLTVGGFRDWLLSYAADSAALAALAPGLTPEMVAAVSKLMRNADLIAVAAKCQVITGFRTTLGLPGRLAS
RLQPNHPTDDPAGIAASTLDGLLFGMGDAVIGINPATDNVGACVTLLEMLDAVRQRFDIPSQSCVLTHVTNSIEAINRGA
PLDLVFQSVAGTEAANAGFGISLSLLGEAREAALSLRRGTVGDNVMYFETGQGAALSADAHHGVDQQTVEVRAYAVCRAF
KPLIVNTVVGFIGPEYLYDSKQIIRAGLEDHCCGKLLGLPMGVDVCYTNHAEADQDDMDTLMTLLGVAGVTFLIGVPGAD
DVMLNYQSLSYHDILGLRHLLDRRPAPEFADWLARMGMSDAGGRLPPLDASAPALRRLLASGG

Sequences:

>Translated_463_residues
MGLYRATVGGTRYDFADLRTVMACASPRRSGDELAGLAAESDAQRMAARLVLADLPLRAFLDTPLVPYESDEVTRLIIDT
HDGAAFAPVASLTVGGFRDWLLSYAADSAALAALAPGLTPEMVAAVSKLMRNADLIAVAAKCQVITGFRTTLGLPGRLAS
RLQPNHPTDDPAGIAASTLDGLLFGMGDAVIGINPATDNVGACVTLLEMLDAVRQRFDIPSQSCVLTHVTNSIEAINRGA
PLDLVFQSVAGTEAANAGFGISLSLLGEAREAALSLRRGTVGDNVMYFETGQGAALSADAHHGVDQQTVEVRAYAVCRAF
KPLIVNTVVGFIGPEYLYDSKQIIRAGLEDHCCGKLLGLPMGVDVCYTNHAEADQDDMDTLMTLLGVAGVTFLIGVPGAD
DVMLNYQSLSYHDILGLRHLLDRRPAPEFADWLARMGMSDAGGRLPPLDASAPALRRLLASGG
>Mature_462_residues
GLYRATVGGTRYDFADLRTVMACASPRRSGDELAGLAAESDAQRMAARLVLADLPLRAFLDTPLVPYESDEVTRLIIDTH
DGAAFAPVASLTVGGFRDWLLSYAADSAALAALAPGLTPEMVAAVSKLMRNADLIAVAAKCQVITGFRTTLGLPGRLASR
LQPNHPTDDPAGIAASTLDGLLFGMGDAVIGINPATDNVGACVTLLEMLDAVRQRFDIPSQSCVLTHVTNSIEAINRGAP
LDLVFQSVAGTEAANAGFGISLSLLGEAREAALSLRRGTVGDNVMYFETGQGAALSADAHHGVDQQTVEVRAYAVCRAFK
PLIVNTVVGFIGPEYLYDSKQIIRAGLEDHCCGKLLGLPMGVDVCYTNHAEADQDDMDTLMTLLGVAGVTFLIGVPGADD
VMLNYQSLSYHDILGLRHLLDRRPAPEFADWLARMGMSDAGGRLPPLDASAPALRRLLASGG

Specific function: Ethanolamine utilization. [C]

COG id: COG4303

COG function: function code E; Ethanolamine ammonia-lyase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082106, Length=441, Percent_Identity=49.2063492063492, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010628 [H]

Pfam domain/function: PF06751 EutB [H]

EC number: =4.3.1.7 [H]

Molecular weight: Translated: 48823; Mature: 48692

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLYRATVGGTRYDFADLRTVMACASPRRSGDELAGLAAESDAQRMAARLVLADLPLRAF
CCCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHH
LDTPLVPYESDEVTRLIIDTHDGAAFAPVASLTVGGFRDWLLSYAADSAALAALAPGLTP
HCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCH
EMVAAVSKLMRNADLIAVAAKCQVITGFRTTLGLPGRLASRLQPNHPTDDPAGIAASTLD
HHHHHHHHHHHCCCEEEEEEHHEEEECHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHH
GLLFGMGDAVIGINPATDNVGACVTLLEMLDAVRQRFDIPSQSCVLTHVTNSIEAINRGA
HHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCC
PLDLVFQSVAGTEAANAGFGISLSLLGEAREAALSLRRGTVGDNVMYFETGQGAALSADA
CHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCC
HHGVDQQTVEVRAYAVCRAFKPLIVNTVVGFIGPEYLYDSKQIIRAGLEDHCCGKLLGLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHCCCCHHHHHHHHCCC
MGVDVCYTNHAEADQDDMDTLMTLLGVAGVTFLIGVPGADDVMLNYQSLSYHDILGLRHL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHH
LDRRPAPEFADWLARMGMSDAGGRLPPLDASAPALRRLLASGG
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC
>Mature Secondary Structure 
GLYRATVGGTRYDFADLRTVMACASPRRSGDELAGLAAESDAQRMAARLVLADLPLRAF
CCEEECCCCCCCCHHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHH
LDTPLVPYESDEVTRLIIDTHDGAAFAPVASLTVGGFRDWLLSYAADSAALAALAPGLTP
HCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCH
EMVAAVSKLMRNADLIAVAAKCQVITGFRTTLGLPGRLASRLQPNHPTDDPAGIAASTLD
HHHHHHHHHHHCCCEEEEEEHHEEEECHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHH
GLLFGMGDAVIGINPATDNVGACVTLLEMLDAVRQRFDIPSQSCVLTHVTNSIEAINRGA
HHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCCC
PLDLVFQSVAGTEAANAGFGISLSLLGEAREAALSLRRGTVGDNVMYFETGQGAALSADA
CHHHHHHHHCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCEECCCC
HHGVDQQTVEVRAYAVCRAFKPLIVNTVVGFIGPEYLYDSKQIIRAGLEDHCCGKLLGLP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHCCHHHHHCCCCHHHHHHHHCCC
MGVDVCYTNHAEADQDDMDTLMTLLGVAGVTFLIGVPGADDVMLNYQSLSYHDILGLRHL
CCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEECCCCHHHHHHHHHH
LDRRPAPEFADWLARMGMSDAGGRLPPLDASAPALRRLLASGG
HHCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]