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The map label for this gene is adh1 [H]

Identifier: 83592240

GI number: 83592240

Start: 1080895

End: 1082055

Strand: Direct

Name: adh1 [H]

Synonym: Rru_A0904

Alternate gene names: 83592240

Gene position: 1080895-1082055 (Clockwise)

Preceding gene: 83592239

Following gene: 83592241

Centisome position: 24.83

GC content: 64.0

Gene sequence:

>1161_bases
ATGCGGGAAAGACAGGCGGCCATGACCGGACTACAGCGCTTCTTCCTGAAAACCGAAATCGTCTTCGGACCAGGCATGTT
CGACCGCCTGGAGGCCTTTCGCGGGCAGCGGATCGGTATCATCACCGATGCCTTCATGGTTAAATCGGGCATGGTCGACC
GGGTGCGGGCCAAGCTTCCTCCCTGTGAGATCAAGGTCTTCGGCGAGGTGGTGCCCGAACCGCCGCTTCAGAACATCGCC
CAGGGCGCGCGTTTCCTCGCCGATTTCAAACCTGACGCCATTTTGGCTTTGGGCGGCGGATCGGCCATCGACGCCGCCAA
AGCGATCTTGGCCACCTTGCGGGAAATGGAGCCGTCGCGCTCCATCGTGATGATCGCCGTGCCGACGACCAGTGGAACCG
GGTCCGAGGTGACGTCTTACGCGATCATTTCCGAGCCGGAGCGGGGGATCAAACACCCCCTGCGCTCCGAAGAGATCATC
CCCGATATCGCGCTGCTCGACCCCGAACTGGTGATGAGCGTTCCGCCGAAAGTTACGGCCGATACCGGCATGGACGTGAT
CACTCACGCGCTTGAAGCCTATGTGGCGCGTGAAGCCAACGACTTCAGCGACGCCTTCGCCGAAAAGGCGCTAGCCCTTG
CCTTCGCCCATCTGCCCACGGCCTTCGCCAACGGCAATGATGTGGTGGCGCGCGGCGCTTTGCATAACGCGTCGTGCATG
GCCGGCATAGCCTTCAGCGCCGCGGGCCTGGGCCTCAACCACGGTTTGGCCCACGCCATCGGTGGCAGGCTGCACATTCC
CCACGGCAAGATCAACGCCATGCTGTTGCCGCTGGTGATCGAATACAACGCCGGCACTCGGGGCGAGTTCGGCCCCTGTC
TGCCCGCCGCGACCCGCTATGCGGAAGTGGCCCGCCGTCTTGGCTTGGAAGGGCCAAGTGTTCGGGCCGGCGTGAAAAGC
TTGGCGCAGGCCATATCCCGTCTGAACGCGCAATTCGGCATCCCGCCGACCCTGCGCGCCCTGGGTGTCGATCTCGACGA
GGTGGCACGTTACGGCGCGGCGATGGTCCAGGGAACCCTGGCCGACAGCTGCACGGCAAGCAATCCGCGAAAGCCTACCC
CGGACGACGTGGCCGAGCTGATCGGCGCGGTTACCGGTTGA

Upstream 100 bases:

>100_bases
TTCGTCCCCCCGGACCAGTCCGGGGGGACGGCCTCCAAGAAGCGGGGTTTTTCGCCAGACGGGGATCCTTCCCCCGGCGG
CGGGCCAACCTCGCCAAGGA

Downstream 100 bases:

>100_bases
GAGTCTCGCCTTCCCGCCAGCGGGAACCAGCGATCCGACAGTGAACACTTGAGAACAAGACGAGGGAAAAACGATGCAAC
AAGAAGCCTTGGGCATGGTG

Product: iron-containing alcohol dehydrogenase

Products: Acetyl-CoA; H+; Nicotinamide adenine dinucleotide - reduced [C]

Alternate protein names: BDH [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MRERQAAMTGLQRFFLKTEIVFGPGMFDRLEAFRGQRIGIITDAFMVKSGMVDRVRAKLPPCEIKVFGEVVPEPPLQNIA
QGARFLADFKPDAILALGGGSAIDAAKAILATLREMEPSRSIVMIAVPTTSGTGSEVTSYAIISEPERGIKHPLRSEEII
PDIALLDPELVMSVPPKVTADTGMDVITHALEAYVAREANDFSDAFAEKALALAFAHLPTAFANGNDVVARGALHNASCM
AGIAFSAAGLGLNHGLAHAIGGRLHIPHGKINAMLLPLVIEYNAGTRGEFGPCLPAATRYAEVARRLGLEGPSVRAGVKS
LAQAISRLNAQFGIPPTLRALGVDLDEVARYGAAMVQGTLADSCTASNPRKPTPDDVAELIGAVTG

Sequences:

>Translated_386_residues
MRERQAAMTGLQRFFLKTEIVFGPGMFDRLEAFRGQRIGIITDAFMVKSGMVDRVRAKLPPCEIKVFGEVVPEPPLQNIA
QGARFLADFKPDAILALGGGSAIDAAKAILATLREMEPSRSIVMIAVPTTSGTGSEVTSYAIISEPERGIKHPLRSEEII
PDIALLDPELVMSVPPKVTADTGMDVITHALEAYVAREANDFSDAFAEKALALAFAHLPTAFANGNDVVARGALHNASCM
AGIAFSAAGLGLNHGLAHAIGGRLHIPHGKINAMLLPLVIEYNAGTRGEFGPCLPAATRYAEVARRLGLEGPSVRAGVKS
LAQAISRLNAQFGIPPTLRALGVDLDEVARYGAAMVQGTLADSCTASNPRKPTPDDVAELIGAVTG
>Mature_386_residues
MRERQAAMTGLQRFFLKTEIVFGPGMFDRLEAFRGQRIGIITDAFMVKSGMVDRVRAKLPPCEIKVFGEVVPEPPLQNIA
QGARFLADFKPDAILALGGGSAIDAAKAILATLREMEPSRSIVMIAVPTTSGTGSEVTSYAIISEPERGIKHPLRSEEII
PDIALLDPELVMSVPPKVTADTGMDVITHALEAYVAREANDFSDAFAEKALALAFAHLPTAFANGNDVVARGALHNASCM
AGIAFSAAGLGLNHGLAHAIGGRLHIPHGKINAMLLPLVIEYNAGTRGEFGPCLPAATRYAEVARRLGLEGPSVRAGVKS
LAQAISRLNAQFGIPPTLRALGVDLDEVARYGAAMVQGTLADSCTASNPRKPTPDDVAELIGAVTG

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=400, Percent_Identity=28.5, Blast_Score=100, Evalue=3e-21,
Organism=Escherichia coli, GI1787493, Length=379, Percent_Identity=37.467018469657, Blast_Score=232, Evalue=3e-62,
Organism=Escherichia coli, GI87082107, Length=368, Percent_Identity=36.9565217391304, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1789163, Length=390, Percent_Identity=33.5897435897436, Blast_Score=194, Evalue=7e-51,
Organism=Escherichia coli, GI48994951, Length=356, Percent_Identity=33.9887640449438, Blast_Score=180, Evalue=1e-46,
Organism=Escherichia coli, GI1789386, Length=354, Percent_Identity=25.7062146892655, Blast_Score=89, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17537053, Length=343, Percent_Identity=27.9883381924198, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6321181, Length=391, Percent_Identity=31.9693094629156, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI24657991, Length=440, Percent_Identity=29.3181818181818, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1; 1.2.1.10 [C]

Molecular weight: Translated: 40707; Mature: 40707

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00913 ADH_IRON_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERQAAMTGLQRFFLKTEIVFGPGMFDRLEAFRGQRIGIITDAFMVKSGMVDRVRAKLP
CCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHCCC
PCEIKVFGEVVPEPPLQNIAQGARFLADFKPDAILALGGGSAIDAAKAILATLREMEPSR
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
SIVMIAVPTTSGTGSEVTSYAIISEPERGIKHPLRSEEIIPDIALLDPELVMSVPPKVTA
CEEEEEEECCCCCCCHHHEEEEECCCHHHHCCCCCCHHCCCCHHHCCHHHHHCCCCCCCC
DTGMDVITHALEAYVAREANDFSDAFAEKALALAFAHLPTAFANGNDVVARGALHNASCM
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
AGIAFSAAGLGLNHGLAHAIGGRLHIPHGKINAMLLPLVIEYNAGTRGEFGPCLPAATRY
HHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHH
AEVARRLGLEGPSVRAGVKSLAQAISRLNAQFGIPPTLRALGVDLDEVARYGAAMVQGTL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
ADSCTASNPRKPTPDDVAELIGAVTG
HHCCCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure
MRERQAAMTGLQRFFLKTEIVFGPGMFDRLEAFRGQRIGIITDAFMVKSGMVDRVRAKLP
CCHHHHHHHHHHHHHHHHHEEECCCHHHHHHHHCCCEEEEEHHHHHHHHCCHHHHHHCCC
PCEIKVFGEVVPEPPLQNIAQGARFLADFKPDAILALGGGSAIDAAKAILATLREMEPSR
CCEEEEECCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCC
SIVMIAVPTTSGTGSEVTSYAIISEPERGIKHPLRSEEIIPDIALLDPELVMSVPPKVTA
CEEEEEEECCCCCCCHHHEEEEECCCHHHHCCCCCCHHCCCCHHHCCHHHHHCCCCCCCC
DTGMDVITHALEAYVAREANDFSDAFAEKALALAFAHLPTAFANGNDVVARGALHNASCM
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHH
AGIAFSAAGLGLNHGLAHAIGGRLHIPHGKINAMLLPLVIEYNAGTRGEFGPCLPAATRY
HHHHHHHHCCCHHHHHHHHCCCEEECCCCCCCEEEEEEEEEECCCCCCCCCCCCHHHHHH
AEVARRLGLEGPSVRAGVKSLAQAISRLNAQFGIPPTLRALGVDLDEVARYGAAMVQGTL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHH
ADSCTASNPRKPTPDDVAELIGAVTG
HHCCCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: Acetaldehyde; Coenzyme A; Nicotinamide adenine dinucleotide [C]

Specific reaction: Acetaldehyde + Coenzyme A + Nicotinamide adenine dinucleotide --> Acetyl-CoA + H+ + Nicotinamide adenine dinucleotide - reduced [C]

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]