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The map label for this gene is pflD [H]

Identifier: 83592239

GI number: 83592239

Start: 1078241

End: 1080781

Strand: Direct

Name: pflD [H]

Synonym: Rru_A0903

Alternate gene names: 83592239

Gene position: 1078241-1080781 (Clockwise)

Preceding gene: 83592238

Following gene: 83592240

Centisome position: 24.77

GC content: 62.06

Gene sequence:

>2541_bases
ATGATCGAAAAAGGTTTCTCCAAGCCGACCGATCGGGTTATGCGGCTGAAGAACGAAATTCTCAACGCCAAGCCCTATGT
CGAGTCCGAGCGGGCGGTTCTGGTCACGGAAGCCTATAAGGAAACGGAAGGGCTGCCGGCCATCCTGCGCCGCGCCAAGG
CCGCCGAGAAGATCTTCAACAATCTGCCGGTGACCATCCGCAACGACGAGTTGATCGTCGGCGCCATCACCAAGAACCCG
CGTTCCACCGAGATCTGCCCGGAATTCTCCTACGACTGGGTCGAAAAAGAATTCGACACCATGGCCACCCGTCTGGCCGA
CCCCTTCCTGATCCCCAAGGAAACCGCCAAGGAACTGCATGACGCGTTCCTGTATTGGCCGGGCAAGACCACCAGCGATC
TCGCCTCTTCCTATATGTCGCAGGAAGCCAAGGATTGCATCGCCTCGGGCGTGTTCACCGTTGGCAACTACTTCTACGGC
GGCGTCGGCCATGTCTGCGTCGACTACGGCAAGGTTCTGAAGATCGGCTTCCGCGGTATCATCACCGAAGTGGTCCAGGC
CATGGAAAAGATGGACCGGATGGACCCGGACTACATCAAGAAGCAGCAGTTCTACAACGCGGTGATCATCGCCTATACGG
CGGCCATCAATTTCGCCCACCGCTATGCGGCGAAGGCCCTGGAACTGGCGCAGAACGAAGCCAACCCGACCCGCAAGGCT
GAGCTGCTTCAGATCGCCCAGAACTGCGCCCGCGTTCCCGAGAACGGCGCCACCACCTTCTATGAGGCCTGCCAGTCCTT
CTGGTTCGTTCAGTGCCTGCTTCAGATCGAGTCCAGCGGCCATTCCATCTCGCCGGGCCGCTTCGACCAGTACATGTATC
CCTTCCTTTGCGCCGACAAGAGCATCGACAAGGGCTTCGCCCAGGAACTCGTCGACTGCATCTGGATCAAGCTCAACGAC
GTCAACAAGACCCGCGACGAAGTGTCGGCCCAGGCCTTCGCCGGTTACGCGGTGTTCCAGAACCTGTGCGTTGGTGGTCA
GACCGAAGGCGGCCTGGATGCGACCAACGAAATCTCCTACATGTGCATGGAGGCCACCGCCCACGTCCGTCTGCCCGCGC
CGTCGTTCTCGATCCGCGTCTGGCAGGGCACGCCCGATGATTTCCTGCACCGCGCCTGTGAAGTGGTCCGCTTGGGCCTT
GGCGTGCCGGCCATGTACAACGACGAAGTCATCGTTCCGGCCCTGCAGAACCGCGGCGTTACCCTGCACGACGCCCGTAA
CTACGGCATCGTCGGCTGCGTGGAGCCCCAGTGCATCCACAAGACCGAAGGCTGGCATGACGCCGCGTTCTTCAACGTCG
CCAAGGTTCTTGAGATCACCCTCAACAACGGCAAGGCCGGCGGCAAGCAGCTTGGTCCGGTGACCGGCGAGTTCACCAGC
TTCCGCAACATGGACGACCTCTACGCGGCCTTCCAGAAGCAGATGGCCTACTTCGTCCATTATCTGGTCGAAGCCGACAA
CTGCGTCGATCTGGCCCATGGCGAGCGTTGCCCGCTGCCCTTCGTCTCGGCCCTGGTCGACGATTGCATCGGTCGCGGCA
AGTCCCTTCAGGAAGGCGGCGCCATCTACAACTTCACCGGTCCCCAGGCCTTCGGCGTCGCCGATACCGGTGACTCGGTC
TATGCCATCCAGAAGAACGTCTTCGAAGACAAGAAGATCACCCTGGCCGAGATGAAGGAAGCGCTCGACGCCAACTTCGG
TCTGCCCGTCGGTGGCTCCGCTCCGTCGGCCGGTGGCGATTTCACCGAGGAGCAGGTGTTCGCCGCGGTCCGCAAGGTCC
TGAGCAGCAACGGCTCGATGGATGTCTCGGCGCTCAAGGGTGAAGTCTACCGCACCCTGTCGGGTCAGGCCGCTCCGGCC
GCCGGCGGCTCGTCGACCAAATACGACGCCATCCGCCGCCTTCTCGATGCCAGCCCGGCCTTCGGCAATGACATCGACGA
CGTCGACATGGTCGCTCGCGAATGCGCCCTGATCTACTGCCGCGAAGTCGAGAAGTACACCAACCCGCGTGGCGGCCAGT
TCCAGGCCGGTATCTACCCGGTGTCGGCGAACGTGCTGTTTGGCAAGGACGTGGCGGCTCTGCCCGATGGCCGTCTGGCC
AAGGCTCCGCTGGCCGATGGCGTCTCCCCGCGTCCCGGTCAGGACGTCAAGGGCCCGACGGCGGCGGCGAATTCGGTGGC
CAAGCTCGACCACTTCATCGCCTCCAACGGTACGCTCTACAACCAGAAGTTCCTGCCGTCGGCCCTGGCCGGGGATGCCG
GACTTCAGAACTTCGCCTCGCTGGTCCGCAGCTACTTCGACCACAAGGGTATGCACGTCCAGTTCAACGTCATCGATCGC
CAGACGCTGCTCGATGCCCAGCTTGAACCGGAAAAGCATAACGACCTGGTCGTTCGCGTCGCCGGCTACAGCGCGCAGTT
CGTCGTCCTCGCCAAGGAAGTGCAAGACGACATCATCAGCCGCACCGAGCAGACCCTCTAG

Upstream 100 bases:

>100_bases
GTTCGGCTTCTGGGCGTGAGGGTTCTGGTCTGACAGGACTTTCCGGTACAGGCCCGGTGGCGAAAAGCGGATCCGTAAAA
ATCCCTATGTGGAGGATGTC

Downstream 100 bases:

>100_bases
CGGCAAACCGGGTTTCGTCCCCCCGGACCAGTCCGGGGGGACGGCCTCCAAGAAGCGGGGTTTTTCGCCAGACGGGGATC
CTTCCCCCGGCGGCGGGCCA

Product: pyruvate formate-lyase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFNNLPVTIRNDELIVGAITKNP
RSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELHDAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYG
GVGHVCVDYGKVLKIGFRGIITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA
ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADKSIDKGFAQELVDCIWIKLND
VNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISYMCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGL
GVPAMYNDEVIVPALQNRGVTLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS
FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGDFTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPA
AGGSSTKYDAIRRLLDASPAFGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA
KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFASLVRSYFDHKGMHVQFNVIDR
QTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIISRTEQTL

Sequences:

>Translated_846_residues
MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFNNLPVTIRNDELIVGAITKNP
RSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELHDAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYG
GVGHVCVDYGKVLKIGFRGIITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA
ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADKSIDKGFAQELVDCIWIKLND
VNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISYMCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGL
GVPAMYNDEVIVPALQNRGVTLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS
FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGDFTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPA
AGGSSTKYDAIRRLLDASPAFGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA
KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFASLVRSYFDHKGMHVQFNVIDR
QTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIISRTEQTL
>Mature_846_residues
MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFNNLPVTIRNDELIVGAITKNP
RSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELHDAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYG
GVGHVCVDYGKVLKIGFRGIITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA
ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADKSIDKGFAQELVDCIWIKLND
VNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISYMCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGL
GVPAMYNDEVIVPALQNRGVTLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS
FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGGAIYNFTGPQAFGVADTGDSV
YAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGDFTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPA
AGGSSTKYDAIRRLLDASPAFGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA
KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFASLVRSYFDHKGMHVQFNVIDR
QTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIISRTEQTL

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=844, Percent_Identity=36.9668246445498, Blast_Score=536, Evalue=1e-153,
Organism=Escherichia coli, GI1787044, Length=858, Percent_Identity=34.032634032634, Blast_Score=485, Evalue=1e-138,
Organism=Escherichia coli, GI1787131, Length=238, Percent_Identity=33.6134453781513, Blast_Score=129, Evalue=1e-30,
Organism=Escherichia coli, GI48994926, Length=238, Percent_Identity=31.9327731092437, Blast_Score=125, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 92730; Mature: 92730

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHC
NLPVTIRNDELIVGAITKNPRSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELH
CCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYGGVGHVCVDYGKVLKIGFRGI
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
SIDKGFAQELVDCIWIKLNDVNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISY
CCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGLGVPAMYNDEVIVPALQNRGV
EEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEHHCCCCE
TLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS
EEEECCCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHH
FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGG
HCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
AIYNFTGPQAFGVADTGDSVYAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGD
CEEECCCCCEECCCCCCCCEEEEHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCC
FTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPAAGGSSTKYDAIRRLLDASPA
CHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
FGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCEEECCCCCCCCCCCCC
KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFAS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECHHHCCHHHCCCCCHHHHHH
LVRSYFDHKGMHVQFNVIDRQTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIIS
HHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHH
RTEQTL
HHHCCC
>Mature Secondary Structure
MIEKGFSKPTDRVMRLKNEILNAKPYVESERAVLVTEAYKETEGLPAILRRAKAAEKIFN
CCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHCCCHHHHHHHHHHHHHHC
NLPVTIRNDELIVGAITKNPRSTEICPEFSYDWVEKEFDTMATRLADPFLIPKETAKELH
CCCEEEECCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
DAFLYWPGKTTSDLASSYMSQEAKDCIASGVFTVGNYFYGGVGHVCVDYGKVLKIGFRGI
HHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
ITEVVQAMEKMDRMDPDYIKKQQFYNAVIIAYTAAINFAHRYAAKALELAQNEANPTRKA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
ELLQIAQNCARVPENGATTFYEACQSFWFVQCLLQIESSGHSISPGRFDQYMYPFLCADK
HHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
SIDKGFAQELVDCIWIKLNDVNKTRDEVSAQAFAGYAVFQNLCVGGQTEGGLDATNEISY
CCCHHHHHHHHHHEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHH
MCMEATAHVRLPAPSFSIRVWQGTPDDFLHRACEVVRLGLGVPAMYNDEVIVPALQNRGV
EEEECCCEEEECCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCEECCCEEEEHHCCCCE
TLHDARNYGIVGCVEPQCIHKTEGWHDAAFFNVAKVLEITLNNGKAGGKQLGPVTGEFTS
EEEECCCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHEEEECCCCCCCCCCCCCCCCHHH
FRNMDDLYAAFQKQMAYFVHYLVEADNCVDLAHGERCPLPFVSALVDDCIGRGKSLQEGG
HCCHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
AIYNFTGPQAFGVADTGDSVYAIQKNVFEDKKITLAEMKEALDANFGLPVGGSAPSAGGD
CEEECCCCCEECCCCCCCCEEEEHHHHCCCCCEEHHHHHHHHCCCCCCCCCCCCCCCCCC
FTEEQVFAAVRKVLSSNGSMDVSALKGEVYRTLSGQAAPAAGGSSTKYDAIRRLLDASPA
CHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCC
FGNDIDDVDMVARECALIYCREVEKYTNPRGGQFQAGIYPVSANVLFGKDVAALPDGRLA
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCEEEEECCEEECCCCCCCCCCCCC
KAPLADGVSPRPGQDVKGPTAAANSVAKLDHFIASNGTLYNQKFLPSALAGDAGLQNFAS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEECHHHCCHHHCCCCCHHHHHH
LVRSYFDHKGMHVQFNVIDRQTLLDAQLEPEKHNDLVVRVAGYSAQFVVLAKEVQDDIIS
HHHHHHCCCCCEEEEEEECCHHHHHCCCCCCCCCCEEEEEECCCEEEEEHHHHHHHHHHH
RTEQTL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]