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The map label for this gene is mdeA [H]

Identifier: 83592113

GI number: 83592113

Start: 924918

End: 926234

Strand: Reverse

Name: mdeA [H]

Synonym: Rru_A0774

Alternate gene names: 83592113

Gene position: 926234-924918 (Counterclockwise)

Preceding gene: 83592125

Following gene: 83592112

Centisome position: 21.28

GC content: 66.97

Gene sequence:

>1317_bases
ATGACCGTTGATGTCGCCCCGCCGAAGGCCGCCCAATACCATCAGGACACCCTGGCCCTTCATGGCGGGTCGTATCGGGC
CGACCCAACCACCGGGGCGGTGGCGGTGCCGATCTATCAAACGACCTCTTATCAGGTTCACGACACCGCCCACGCGGCGC
GGTTGGCCGGGCTGGAGGAGATCGGCTTCGCCTATACCCGTATCGGCAATCCAACCCAGGACGCCTTTGAACAAAGGCTG
GCCGCCCTGGAAGGCGGGGTGGCGGCCCTGGCCCTGGCCTCGGGTCAAGCGGCCTCGGCGATCGCTGTGCTGACCCTGGC
CCAGGCCGGCGATAATATCGTCACCTCGACCGATCTTTACGGCGGCACCTGGAATTTGTTCGCCAATACCCTGCGCACGC
TGGGGATCGAGGCGCGCTTCGTCGATCCGGCCGATCCCGAGGCCTTCCGCCGCGCCACCGACGCGCGGACCCGCCTCTAC
TATGGCGAGACCCTGCCCAATCCGAAGCTGACGGTTTTCCCGATCGCCGAGGTCGCCGCCATCGGCCGCTCGCTTGGCGT
GCCGCTGATCATCGATAATACGGCGACGCCGCTGATCGCCCGGCCCTTCGACCATGGGGCGGCGGTGGTCGTCTATTCCA
CCACCAAATACATCGGCGGCCATGGCACCACCATCGGCGGGGCGATCATCGATGGCGGCACCTTCCCCTGGGCCGACCAC
GCCGAGCGCCATGCCCTGCTGACCGGCCCCGAGCCCAGCTATCACAATGCCAGCTGGACCGATCTGGCCGCCCCCTTCGG
CCCGCTCGCCTATATCCTGCGCGCCCGCGCCGGGGTATTGCGCGATCTTGGCGCCAGCATCGCCCCGGTCAGCGCCTTCA
ACCTGATCCAGGGGCTGGAAACCCTGCCGCTGCGTCTGGCCCGCCATAACGCCAACGCCATCAAGGTCGCCGATTATCTG
GCCAGCCATCCCAAGGTCAGCTCGGTAACCTTTCCCGGGTTGCAAAGCGGCGAGGCCCGGCGGCGGGCCGATGCCTATCT
CAAGGGCGGCTATGGCGGGCTGATCGGCTTTGAGTTGGCCGGTGGCGAGGAAGCCGGGCGGCGGTTCATCGAAGCCCTGC
GGCTGTTCTACCACGTCTTCAATATCGGCGACGCCCGCTCGCTGGCGATCCATCCGGCGACCACCACCCATTCCCAGCTC
TCGCCCGAAGACCAGCGCGCCACCGGCGTCACCCCGGGCTTCGTGCGGCTGTCGATCGGCATCGAGCATCCCGACGACAT
CATCGGCGACATCGCCCAAGCCTTGGATCTGGTTTGA

Upstream 100 bases:

>100_bases
TATAACTAAACAAAAATTCTATTTTTTCTACATGGAAATCCTGGCGAAAACCTCCTCCCATAGGGTGATGTTCTGCCCAC
CCGTCACCGGAGGATTGACC

Downstream 100 bases:

>100_bases
TTTTCTTTCCGCTTTAAGGAGGCGTCCCTGGTGACAAAGATCGAAAAGCCGCTCCAGCCGGTCGAAATCCGCGAACGACG
GCTGGGCTCGATCACCGGCT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 438; Mature: 437

Protein sequence:

>438_residues
MTVDVAPPKAAQYHQDTLALHGGSYRADPTTGAVAVPIYQTTSYQVHDTAHAARLAGLEEIGFAYTRIGNPTQDAFEQRL
AALEGGVAALALASGQAASAIAVLTLAQAGDNIVTSTDLYGGTWNLFANTLRTLGIEARFVDPADPEAFRRATDARTRLY
YGETLPNPKLTVFPIAEVAAIGRSLGVPLIIDNTATPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGAIIDGGTFPWADH
AERHALLTGPEPSYHNASWTDLAAPFGPLAYILRARAGVLRDLGASIAPVSAFNLIQGLETLPLRLARHNANAIKVADYL
ASHPKVSSVTFPGLQSGEARRRADAYLKGGYGGLIGFELAGGEEAGRRFIEALRLFYHVFNIGDARSLAIHPATTTHSQL
SPEDQRATGVTPGFVRLSIGIEHPDDIIGDIAQALDLV

Sequences:

>Translated_438_residues
MTVDVAPPKAAQYHQDTLALHGGSYRADPTTGAVAVPIYQTTSYQVHDTAHAARLAGLEEIGFAYTRIGNPTQDAFEQRL
AALEGGVAALALASGQAASAIAVLTLAQAGDNIVTSTDLYGGTWNLFANTLRTLGIEARFVDPADPEAFRRATDARTRLY
YGETLPNPKLTVFPIAEVAAIGRSLGVPLIIDNTATPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGAIIDGGTFPWADH
AERHALLTGPEPSYHNASWTDLAAPFGPLAYILRARAGVLRDLGASIAPVSAFNLIQGLETLPLRLARHNANAIKVADYL
ASHPKVSSVTFPGLQSGEARRRADAYLKGGYGGLIGFELAGGEEAGRRFIEALRLFYHVFNIGDARSLAIHPATTTHSQL
SPEDQRATGVTPGFVRLSIGIEHPDDIIGDIAQALDLV
>Mature_437_residues
TVDVAPPKAAQYHQDTLALHGGSYRADPTTGAVAVPIYQTTSYQVHDTAHAARLAGLEEIGFAYTRIGNPTQDAFEQRLA
ALEGGVAALALASGQAASAIAVLTLAQAGDNIVTSTDLYGGTWNLFANTLRTLGIEARFVDPADPEAFRRATDARTRLYY
GETLPNPKLTVFPIAEVAAIGRSLGVPLIIDNTATPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGAIIDGGTFPWADHA
ERHALLTGPEPSYHNASWTDLAAPFGPLAYILRARAGVLRDLGASIAPVSAFNLIQGLETLPLRLARHNANAIKVADYLA
SHPKVSSVTFPGLQSGEARRRADAYLKGGYGGLIGFELAGGEEAGRRFIEALRLFYHVFNIGDARSLAIHPATTTHSQLS
PEDQRATGVTPGFVRLSIGIEHPDDIIGDIAQALDLV

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=409, Percent_Identity=30.8068459657702, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI25453487, Length=424, Percent_Identity=27.5943396226415, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI299473759, Length=426, Percent_Identity=27.4647887323944, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1790375, Length=419, Percent_Identity=29.3556085918854, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789383, Length=413, Percent_Identity=24.9394673123487, Blast_Score=102, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI71980708, Length=424, Percent_Identity=30.4245283018868, Blast_Score=178, Evalue=6e-45,
Organism=Caenorhabditis elegans, GI17560056, Length=428, Percent_Identity=31.7757009345794, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI17538055, Length=428, Percent_Identity=31.3084112149533, Blast_Score=177, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6323333, Length=438, Percent_Identity=43.37899543379, Blast_Score=319, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6319307, Length=430, Percent_Identity=31.6279069767442, Blast_Score=161, Evalue=1e-40,
Organism=Saccharomyces cerevisiae, GI6321170, Length=360, Percent_Identity=24.7222222222222, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19922554, Length=426, Percent_Identity=31.6901408450704, Blast_Score=194, Evalue=1e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46313; Mature: 46181

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.2 %Met     (Translated Protein)
0.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVDVAPPKAAQYHQDTLALHGGSYRADPTTGAVAVPIYQTTSYQVHDTAHAARLAGLEE
CEECCCCCCCHHHCCCEEEEECCCEECCCCCCEEEEEEEECCCEEEECHHHHHHHCCHHH
IGFAYTRIGNPTQDAFEQRLAALEGGVAALALASGQAASAIAVLTLAQAGDNIVTSTDLY
HCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEEEHHHCCCCEEEECCCC
GGTWNLFANTLRTLGIEARFVDPADPEAFRRATDARTRLYYGETLPNPKLTVFPIAEVAA
CCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHH
IGRSLGVPLIIDNTATPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGAIIDGGTFPWADH
HHHHCCCCEEECCCCCCEEECCCCCCCEEEEEEEEEEECCCCCEECCEEECCCCCCCCCC
AERHALLTGPEPSYHNASWTDLAAPFGPLAYILRARAGVLRDLGASIAPVSAFNLIQGLE
CCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TLPLRLARHNANAIKVADYLASHPKVSSVTFPGLQSGEARRRADAYLKGGYGGLIGFELA
HHHHHHHHCCCCEEEHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHEECCCCCEEEEEEC
GGEEAGRRFIEALRLFYHVFNIGDARSLAIHPATTTHSQLSPEDQRATGVTPGFVRLSIG
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCCEEEEEEE
IEHPDDIIGDIAQALDLV
CCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVDVAPPKAAQYHQDTLALHGGSYRADPTTGAVAVPIYQTTSYQVHDTAHAARLAGLEE
EECCCCCCCHHHCCCEEEEECCCEECCCCCCEEEEEEEECCCEEEECHHHHHHHCCHHH
IGFAYTRIGNPTQDAFEQRLAALEGGVAALALASGQAASAIAVLTLAQAGDNIVTSTDLY
HCCHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCHHHEEEEEHHHCCCCEEEECCCC
GGTWNLFANTLRTLGIEARFVDPADPEAFRRATDARTRLYYGETLPNPKLTVFPIAEVAA
CCHHHHHHHHHHHHCCEEEECCCCCHHHHHHHHHHHHEEEECCCCCCCCEEEEEHHHHHH
IGRSLGVPLIIDNTATPLIARPFDHGAAVVVYSTTKYIGGHGTTIGGAIIDGGTFPWADH
HHHHCCCCEEECCCCCCEEECCCCCCCEEEEEEEEEEECCCCCEECCEEECCCCCCCCCC
AERHALLTGPEPSYHNASWTDLAAPFGPLAYILRARAGVLRDLGASIAPVSAFNLIQGLE
CCCCEEEECCCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
TLPLRLARHNANAIKVADYLASHPKVSSVTFPGLQSGEARRRADAYLKGGYGGLIGFELA
HHHHHHHHCCCCEEEHHHHHHCCCCCCEEECCCCCCCCHHHHHHHHEECCCCCEEEEEEC
GGEEAGRRFIEALRLFYHVFNIGDARSLAIHPATTTHSQLSPEDQRATGVTPGFVRLSIG
CCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCHHCCCCCCCEEEEEEE
IEHPDDIIGDIAQALDLV
CCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]