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The map label for this gene is prfA

Identifier: 83592089

GI number: 83592089

Start: 879011

End: 880075

Strand: Direct

Name: prfA

Synonym: Rru_A0750

Alternate gene names: 83592089

Gene position: 879011-880075 (Clockwise)

Preceding gene: 83592088

Following gene: 83592090

Centisome position: 20.19

GC content: 68.08

Gene sequence:

>1065_bases
ATGAGTTTGGAAGAGAACCTGAGCCGCGTGGTCGCCCGGCACGATGAATTGCGCGCCCTGCTGTCGACCAGCGCCGGCGG
CGGCGAGGGCTTCGTGCGCATGTCGCGCGAATTGGCCGATCTCGCCCCGGTGGTCGAGGCGATCGGCCGGCTTGATGACG
CCAAGGCCGAGTTGGCCGGGGCGCGGGCCCTGCTCGACGATCCCGACATGCGCGATCTCGCCCGCGAGGAGGTGCTGCGG
CTGGAAGCCGAGATCCCCGCCCTTGAACACGGGGTGAAGCTTCTGCTGCTGCCCCGCGACGAGGCCGACGACCGTGGCGT
GATCCTTGAGGTGCGCGCCGGAACCGGCGGCGACGAGGCGGCGCTGTTCGCCGGAGATCTGTTGCGCATGTACGAACGCT
ATGCCCAGGAACGCGGCTGGCGGTTCGAGGTGATGGAGGCCAGCGATACGGATATCGGCGGCATCAAGGAAGCCAGCGCG
GCGATTTCCGGTCGCGGGGTTTTCGCTCGGCTCAAGTTCGAATCCGGGGTTCATCGGGTGCAGCGGGTGCCGGTGACCGA
AGGCGGCGGACGCATCCACACCTCGGCGGCGACGGTGGCGGTGCTGCCCGAGGCCGAGGAGGTCGATGTCGAGATCGAGG
AAAAGGACCTGCGCATCGATACCTATCGCTCGCAGGGCGCCGGCGGCCAGCACGTCAACACCACCGATAGCGCCGTGCGC
ATCACCCATCTGCCCAGCGGCGTCGTCGCCCAGTGCCAGGATGAAAAATCCCAGCACAAGAACAAGGCCAAGGCGATGCG
CATGCTGCGCACCAAGCTTTATGACCACGCCCGGCAGACGGCGGATGACGCCCGGGCCGAGGCGCGGCGCGTTCAGGTGG
GCTCGGGCGATCGCTCCGAACGCATCCGCACCTATAACTTCCCGCAGGGCCGGGTGACCGACCACCGCATCGGCCTGACC
CTGCACCGCTTGCCCGAGGTTCTGGCCGGAACCGCCCTTGACGAGGTGATCGAGCCGCTGATCGCCGAGGATCAGGCGAC
GCGGATGGCGGAGATCGGCGGGTGA

Upstream 100 bases:

>100_bases
TCATGACGGTGCGGGCGGAGATGCTGCTGCGGCGGCTGTTTGACCTGCCCACGAGCATCGCCGAAACGGGCGCAACCGAC
CGGGAGGATGGGTCGAAGGG

Downstream 100 bases:

>100_bases
GCGACGGCGACGGCGGCGGGGAGCAAGGGGGCGGCGGTCCGGCCGAGGCGGAGGAGTCCGCCCTGGTATTGGGCCGGTTG
CTCGACCGTGGCGCCTGGCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1

Number of amino acids: Translated: 354; Mature: 353

Protein sequence:

>354_residues
MSLEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAGARALLDDPDMRDLAREEVLR
LEAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGDEAALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASA
AISGRGVFARLKFESGVHRVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSAVR
ITHLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDRSERIRTYNFPQGRVTDHRIGLT
LHRLPEVLAGTALDEVIEPLIAEDQATRMAEIGG

Sequences:

>Translated_354_residues
MSLEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAGARALLDDPDMRDLAREEVLR
LEAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGDEAALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASA
AISGRGVFARLKFESGVHRVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSAVR
ITHLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDRSERIRTYNFPQGRVTDHRIGLT
LHRLPEVLAGTALDEVIEPLIAEDQATRMAEIGG
>Mature_353_residues
SLEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAGARALLDDPDMRDLAREEVLRL
EAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGDEAALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASAA
ISGRGVFARLKFESGVHRVQRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSAVRI
THLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDRSERIRTYNFPQGRVTDHRIGLTL
HRLPEVLAGTALDEVIEPLIAEDQATRMAEIGG

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=337, Percent_Identity=48.0712166172107, Blast_Score=308, Evalue=4e-84,
Organism=Homo sapiens, GI34577120, Length=357, Percent_Identity=40.6162464985994, Blast_Score=264, Evalue=9e-71,
Organism=Homo sapiens, GI166795305, Length=242, Percent_Identity=45.8677685950413, Blast_Score=209, Evalue=4e-54,
Organism=Escherichia coli, GI1787462, Length=346, Percent_Identity=56.3583815028902, Blast_Score=357, Evalue=1e-100,
Organism=Escherichia coli, GI2367172, Length=311, Percent_Identity=38.5852090032154, Blast_Score=206, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17542784, Length=298, Percent_Identity=37.9194630872483, Blast_Score=207, Evalue=9e-54,
Organism=Saccharomyces cerevisiae, GI6321295, Length=334, Percent_Identity=42.814371257485, Blast_Score=249, Evalue=4e-67,
Organism=Drosophila melanogaster, GI19921226, Length=345, Percent_Identity=42.6086956521739, Blast_Score=248, Evalue=4e-66,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): RF1_RHORT (Q2RWE1)

Other databases:

- EMBL:   CP000230
- RefSeq:   YP_425841.1
- HSSP:   P0A7I0
- ProteinModelPortal:   Q2RWE1
- SMR:   Q2RWE1
- STRING:   Q2RWE1
- GeneID:   3834159
- GenomeReviews:   CP000230_GR
- KEGG:   rru:Rru_A0750
- NMPDR:   fig|1085.1.peg.1595
- eggNOG:   COG0216
- HOGENOM:   HBG629764
- OMA:   SEQGGYK
- PhylomeDB:   Q2RWE1
- BioCyc:   RRUB269796:RRU_A0750-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00093
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373
- SMART:   SM00937
- TIGRFAMs:   TIGR00019

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 38736; Mature: 38605

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAG
CCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ARALLDDPDMRDLAREEVLRLEAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGDEA
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCHH
ALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASAAISGRGVFARLKFESGVHRV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHH
QRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSAVR
HCCCCCCCCCEEEECCEEEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCEEE
ITHLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDRSE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
RIRTYNFPQGRVTDHRIGLTLHRLPEVLAGTALDEVIEPLIAEDQATRMAEIGG
CCEEECCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure 
SLEENLSRVVARHDELRALLSTSAGGGEGFVRMSRELADLAPVVEAIGRLDDAKAELAG
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
ARALLDDPDMRDLAREEVLRLEAEIPALEHGVKLLLLPRDEADDRGVILEVRAGTGGDEA
HHHHCCCCCHHHHHHHHHHHHHHCCCHHHCCCEEEEEECCCCCCCCEEEEEECCCCCCHH
ALFAGDLLRMYERYAQERGWRFEVMEASDTDIGGIKEASAAISGRGVFARLKFESGVHRV
HHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHCCCCEEEEEEHHHHHHHH
QRVPVTEGGGRIHTSAATVAVLPEAEEVDVEIEEKDLRIDTYRSQGAGGQHVNTTDSAVR
HCCCCCCCCCEEEECCEEEEECCCCCCCCEEEECCCCEEEECCCCCCCCCCCCCCCCEEE
ITHLPSGVVAQCQDEKSQHKNKAKAMRMLRTKLYDHARQTADDARAEARRVQVGSGDRSE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCC
RIRTYNFPQGRVTDHRIGLTLHRLPEVLAGTALDEVIEPLIAEDQATRMAEIGG
CCEEECCCCCCCCCHHHCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA